• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of plntphysLink to Publisher's site
Plant Physiol. Mar 1996; 110(3): 987–996.
PMCID: PMC157799

Abscisic Acid-Induced Phosphoinositide Turnover in Guard Cell Protoplasts of Vicia faba.

Abstract

Guard cell protoplasts of Vicia faba treated with 10 [mu]M (+)abscisic acid (ABA) in the light exhibited a 20% decrease in diameter within 1.5 h, from 24.1 to 19.6 [mu]m. Within 10 s of administration of ABA, a 90% increase in levels of inositol 1,4,5-trisphosphate was observed, provided that cells were treated with Li+, an inhibitor of inositol phosphatase activity, prior to incubation. Concomitantly, levels of 32P-labeled phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 4-phosphate decreased 20% compared to levels in control cells; levels of label in the membrane lipids phosphatidylcholine, phosphatidylethanolamine, and phosphatidylglycerol did not change significantly in response to ABA treatment. These results show that phosphoinositide turnover is activated in response to ABA in guard cells. We conclude that phosphoinositide signaling is likely to be a step in the biochemical cascade that couples ABA to guard cell shrinking and stomatal closure.

Full Text

The Full Text of this article is available as a PDF (1.4M).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Allan AC, Fricker MD, Ward JL, Beale MH, Trewavas AJ. Two Transduction Pathways Mediate Rapid Effects of Abscisic Acid in Commelina Guard Cells. Plant Cell. 1994 Sep;6(9):1319–1328. [PMC free article] [PubMed]
  • Anderson BE, Ward JM, Schroeder JI. Evidence for an Extracellular Reception Site for Abscisic Acid in Commelina Guard Cells. Plant Physiol. 1994 Apr;104(4):1177–1183. [PMC free article] [PubMed]
  • BARTLETT GR. Phosphorus assay in column chromatography. J Biol Chem. 1959 Mar;234(3):466–468. [PubMed]
  • Berridge MJ. Inositol trisphosphate and calcium signalling. Nature. 1993 Jan 28;361(6410):315–325. [PubMed]
  • Blatt MR, Thiel G, Trentham DR. Reversible inactivation of K+ channels of Vicia stomatal guard cells following the photolysis of caged inositol 1,4,5-trisphosphate. Nature. 1990 Aug 23;346(6286):766–769. [PubMed]
  • Carpenter CL, Cantley LC. Phosphoinositide kinases. Biochemistry. 1990 Dec 25;29(51):11147–11156. [PubMed]
  • Challiss RA, Chilvers ER, Willcocks AL, Nahorski SR. Heterogeneity of [3H]inositol 1,4,5-trisphosphate binding sites in adrenal-cortical membranes. Characterization and validation of a radioreceptor assay. Biochem J. 1990 Jan 15;265(2):421–427. [PMC free article] [PubMed]
  • Coté GG, Crain RC. Artifactual Elevation of the Apparent Levels of Phosphatidic Acid and Phosphatidylinositol 4,5-Bisphosphate during Short-Term Labeling of Plant Tissue with Radioactive Precursor. Plant Physiol. 1992 Oct;100(2):1042–1043. [PMC free article] [PubMed]
  • Coté GG, Depass AL, Quarmby LM, Tate BF, Morse MJ, Satter RL, Crain RC. Separation and Characterization of Inositol Phospholipids from the Pulvini of Samanea saman. Plant Physiol. 1989 Aug;90(4):1422–1428. [PMC free article] [PubMed]
  • Downes CP, Mussat MC, Michell RH. The inositol trisphosphate phosphomonoesterase of the human erythrocyte membrane. Biochem J. 1982 Apr 1;203(1):169–177. [PMC free article] [PubMed]
  • Drobak BK. Plant Phosphoinositides and Intracellular Signaling. Plant Physiol. 1993 Jul;102(3):705–709. [PMC free article] [PubMed]
  • Fairley-Grenot K, Assmann SM. Evidence for G-Protein Regulation of Inward K+ Channel Current in Guard Cells of Fava Bean. Plant Cell. 1991 Sep;3(9):1037–1044. [PMC free article] [PubMed]
  • Fukami K, Takenawa T. Quantitative changes in polyphosphoinositides 1,2-diacylglycerol and inositol 1,4,5-trisphosphate by platelet-derived growth factor and prostaglandin F2 alpha. J Biol Chem. 1989 Sep 5;264(25):14985–14989. [PubMed]
  • Gillaspy GE, Keddie JS, Oda K, Gruissem W. Plant inositol monophosphatase is a lithium-sensitive enzyme encoded by a multigene family. Plant Cell. 1995 Dec;7(12):2175–2185. [PMC free article] [PubMed]
  • Gilroy S, Fricker MD, Read ND, Trewavas AJ. Role of Calcium in Signal Transduction of Commelina Guard Cells. Plant Cell. 1991 Apr;3(4):333–344. [PMC free article] [PubMed]
  • Gilroy S, Read ND, Trewavas AJ. Elevation of cytoplasmic calcium by caged calcium or caged inositol triphosphate initiates stomatal closure. Nature. 1990 Aug 23;346(6286):769–771. [PubMed]
  • Hallcher LM, Sherman WR. The effects of lithium ion and other agents on the activity of myo-inositol-1-phosphatase from bovine brain. J Biol Chem. 1980 Nov 25;255(22):10896–10901. [PubMed]
  • Hartzell LB, Hartzell HC, Quarmby LM. Mechanisms of flagellar excision. I. The role of intracellular acidification. Exp Cell Res. 1993 Sep;208(1):148–153. [PubMed]
  • Harvey BE, Crain RC. Biosynthesis of glycerolipids by hepatoma and liver microsomes. I. Fatty acyl-CoA ligase and acyl-CoA:sn-glycerol-3-phosphate acyltransferase. Biochim Biophys Acta. 1987 Feb 14;917(2):247–257. [PubMed]
  • Hirayama T, Ohto C, Mizoguchi T, Shinozaki K. A gene encoding a phosphatidylinositol-specific phospholipase C is induced by dehydration and salt stress in Arabidopsis thaliana. Proc Natl Acad Sci U S A. 1995 Apr 25;92(9):3903–3907. [PMC free article] [PubMed]
  • Inhorn RC, Majerus PW. Inositol polyphosphate 1-phosphatase from calf brain. Purification and inhibition by Li+, Ca2+, and Mn2+. J Biol Chem. 1987 Nov 25;262(33):15946–15952. [PubMed]
  • Irvine RF. Inositol phosphates and Ca2+ entry: toward a proliferation or a simplification? FASEB J. 1992 Sep;6(12):3085–3091. [PubMed]
  • Kim HY, Cote GG, Crain RC. Inositol 1,4,5-trisphosphate may mediate closure of K+ channels by light and darkness in Samanea saman motor cells. Planta. 1996 Feb;198(2):279–287. [PubMed]
  • Kirk CJ, Hunt PA, Michell RH. Do cells contain discrete pools of inositol lipids that are coupled to receptor activation? Biochem Soc Trans. 1989 Dec;17(6):978–980. [PubMed]
  • Kruse T, Tallman G, Zeiger E. Isolation of Guard Cell Protoplasts from Mechanically Prepared Epidermis of Vicia faba Leaves. Plant Physiol. 1989 Aug;90(4):1382–1386. [PMC free article] [PubMed]
  • Legendre L, Yueh YG, Crain R, Haddock N, Heinstein PF, Low PS. Phospholipase C activation during elicitation of the oxidative burst in cultured plant cells. J Biol Chem. 1993 Nov 25;268(33):24559–24563. [PubMed]
  • Lemtiri-Chlieh F, MacRobbie EA. Role of calcium in the modulation of Vicia guard cell potassium channels by abscisic acid: a patch-clamp study. J Membr Biol. 1994 Jan;137(2):99–107. [PubMed]
  • Martinoia E, Locher R, Vogt E. Inositol Trisphosphate Metabolism in Subcellular Fractions of Barley (Hordeum vulgare L.) Mesophyll Cells. Plant Physiol. 1993 May;102(1):101–105. [PMC free article] [PubMed]
  • McAinsh MR, Brownlee C, Hetherington AM. Visualizing Changes in Cytosolic-Free Ca2+ during the Response of Stomatal Guard Cells to Abscisic Acid. Plant Cell. 1992 Sep;4(9):1113–1122. [PMC free article] [PubMed]
  • McAinsh MR, Webb AAR, Taylor JE, Hetherington AM. Stimulus-Induced Oscillations in Guard Cell Cytosolic Free Calcium. Plant Cell. 1995 Aug;7(8):1207–1219. [PMC free article] [PubMed]
  • Mignery GA, Südhof TC. The ligand binding site and transduction mechanism in the inositol-1,4,5-triphosphate receptor. EMBO J. 1990 Dec;9(12):3893–3898. [PMC free article] [PubMed]
  • Morse MJ, Crain RC, Satter RL. Light-stimulated inositolphospholipid turnover in Samanea saman leaf pulvini. Proc Natl Acad Sci U S A. 1987 Oct;84(20):7075–7078. [PMC free article] [PubMed]
  • Neuhaus G, Bowler C, Kern R, Chua NH. Calcium/calmodulin-dependent and -independent phytochrome signal transduction pathways. Cell. 1993 Jun 4;73(5):937–952. [PubMed]
  • Quarmby LM, Yueh YG, Cheshire JL, Keller LR, Snell WJ, Crain RC. Inositol phospholipid metabolism may trigger flagellar excision in Chlamydomonas reinhardtii. J Cell Biol. 1992 Feb;116(3):737–744. [PMC free article] [PubMed]
  • Rana RS, Hokin LE. Role of phosphoinositides in transmembrane signaling. Physiol Rev. 1990 Jan;70(1):115–164. [PubMed]
  • Schroeder JI, Hagiwara S. Repetitive increases in cytosolic Ca2+ of guard cells by abscisic acid activation of nonselective Ca2+ permeable channels. Proc Natl Acad Sci U S A. 1990 Dec;87(23):9305–9309. [PMC free article] [PubMed]
  • Schwartz A, Wu WH, Tucker EB, Assmann SM. Inhibition of inward K+ channels and stomatal response by abscisic acid: an intracellular locus of phytohormone action. Proc Natl Acad Sci U S A. 1994 Apr 26;91(9):4019–4023. [PMC free article] [PubMed]
  • Shimazaki K, Zeiger E. Cyclic and Noncyclic Photophosphorylation in Isolated Guard Cell Chloroplasts from Vicia faba L. Plant Physiol. 1985 Jun;78(2):211–214. [PMC free article] [PubMed]
  • Van der Kaay J, Draijer R, Van Haastert PJ. Increased conversion of phosphatidylinositol to phosphatidylinositol phosphate in Dictyostelium cells expressing a mutated ras gene. Proc Natl Acad Sci U S A. 1990 Dec;87(23):9197–9201. [PMC free article] [PubMed]
  • Yamamoto YT, Conkling MA, Sussex IM, Irish VF. An Arabidopsis cDNA related to animal phosphoinositide-specific phospholipase C genes. Plant Physiol. 1995 Mar;107(3):1029–1030. [PMC free article] [PubMed]
  • Yueh YG, Crain RC. Deflagellation of Chlamydomonas reinhardtii follows a rapid transitory accumulation of inositol 1,4,5-trisphosphate and requires Ca2+ entry. J Cell Biol. 1993 Nov;123(4):869–875. [PMC free article] [PubMed]

Articles from Plant Physiology are provided here courtesy of American Society of Plant Biologists

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...