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Plant Physiol. Jan 1996; 110(1): 249–257.
PMCID: PMC157716

Expression of a Late Embryogenesis Abundant Protein Gene, HVA1, from Barley Confers Tolerance to Water Deficit and Salt Stress in Transgenic Rice.


A late embryogenesis abundant (LEA) protein gene, HVA1, from barley (Hordeum vulgare L.) was introduced into rice suspension cells using the Biolistic-mediated transformation method, and a large number of independent transgenic rice (Oryza sativa L.) plants were generated. Expression of the barley HVA1 gene regulated by the rice actin 1 gene promoter led to high-level, constitutive accumulation of the HVA1 protein in both leaves and roots of transgenic rice plants. Second-generation transgenic rice plants showed significantly increased tolerance to water deficit and salinity. Transgenic rice plants maintained higher growth rates than nontransformed control plants under stress conditions. The increased tolerance was also reflected by delayed development of damage symptoms caused by stress and by improved recovery upon the removal of stress conditions. We also found that the extent of increased stress tolerance correlated with the level of the HVA1 protein accumulated in the transgenic rice plants. Using a transgenic approach, this study provides direct evidence supporting the hypothesis that LEA proteins play an important role in the protection of plants under water-or salt-stress conditions. Thus, LEA genes hold considerable potential for use as molecular tools for genetic crop improvement toward stress tolerance.

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Selected References

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  • Blackman SA, Obendorf RL, Leopold AC. Maturation proteins and sugars in desiccation tolerance of developing soybean seeds. Plant Physiol. 1992 Sep;100(1):225–230. [PMC free article] [PubMed]
  • Blackman SA, Wettlaufer SH, Obendorf RL, Leopold AC. Maturation proteins associated with desiccation tolerance in soybean. Plant Physiol. 1991 Jul;96(3):868–874. [PMC free article] [PubMed]
  • Bradford KJ, Chandler PM. Expression of "Dehydrin-Like" Proteins in Embryos and Seedlings of Zizania palustris and Oryza sativa during Dehydration. Plant Physiol. 1992 Jun;99(2):488–494. [PMC free article] [PubMed]
  • Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. [PubMed]
  • Close TJ, Fenton RD, Moonan F. A view of plant dehydrins using antibodies specific to the carboxy terminal peptide. Plant Mol Biol. 1993 Oct;23(2):279–286. [PubMed]
  • Curry J, Walker-Simmons MK. Unusual sequence of group 3 LEA (II) mRNA inducible by dehydration stress in wheat. Plant Mol Biol. 1993 Mar;21(5):907–912. [PubMed]
  • Dure L., 3rd A repeating 11-mer amino acid motif and plant desiccation. Plant J. 1993 Mar;3(3):363–369. [PubMed]
  • Epstein E, Norlyn JD, Rush DW, Kingsbury RW, Kelley DB, Cunningham GA, Wrona AF. Saline culture of crops: a genetic approach. Science. 1980 Oct 24;210(4468):399–404. [PubMed]
  • Holmström KO, Welin B, Mandal A, Kristiansdottir I, Teeri TH, Lamark T, Strøm AR, Palva ET. Production of the Escherichia coli betaine-aldehyde dehydrogenase, an enzyme required for the synthesis of the osmoprotectant glycine betaine, in transgenic plants. Plant J. 1994 Nov;6(5):749–758. [PubMed]
  • Hong B, Barg R, Ho TH. Developmental and organ-specific expression of an ABA- and stress-induced protein in barley. Plant Mol Biol. 1992 Feb;18(4):663–674. [PubMed]
  • Moons A, Bauw G, Prinsen E, Van Montagu M, Van der Straeten D. Molecular and physiological responses to abscisic acid and salts in roots of salt-sensitive and salt-tolerant Indica rice varieties. Plant Physiol. 1995 Jan;107(1):177–186. [PMC free article] [PubMed]
  • Mundy J, Chua NH. Abscisic acid and water-stress induce the expression of a novel rice gene. EMBO J. 1988 Aug;7(8):2279–2286. [PMC free article] [PubMed]
  • Rathinasabapathi B, McCue KF, Gage DA, Hanson AD. Metabolic engineering of glycine betaine synthesis: plant betaine aldehyde dehydrogenases lacking typical transit peptides are targeted to tobacco chloroplasts where they confer betaine aldehyde resistance. Planta. 1994;193(2):155–162. [PubMed]
  • Ried JL, Walker-Simmons MK. Group 3 Late Embryogenesis Abundant Proteins in Desiccation-Tolerant Seedlings of Wheat (Triticum aestivum L.). Plant Physiol. 1993 May;102(1):125–131. [PMC free article] [PubMed]
  • Saneoka H, Nagasaka C, Hahn DT, Yang WJ, Premachandra GS, Joly RJ, Rhodes D. Salt Tolerance of Glycinebetaine-Deficient and -Containing Maize Lines. Plant Physiol. 1995 Feb;107(2):631–638. [PMC free article] [PubMed]
  • Seffens WS, Almoguera C, Wilde HD, Vonder Haar RA, Thomas TL. Molecular analysis of a phylogenetically conserved carrot gene: developmental and environmental regulation. Dev Genet. 1990;11(1):65–76. [PubMed]
  • Skriver K, Mundy J. Gene expression in response to abscisic acid and osmotic stress. Plant Cell. 1990 Jun;2(6):503–512. [PMC free article] [PubMed]
  • Still DW, Kovach DA, Bradford KJ. Development of Desiccation Tolerance during Embryogenesis in Rice (Oryza sativa) and Wild Rice (Zizania palustris) (Dehydrin Expression, Abscisic Acid Content, and Sucrose Accumulation). Plant Physiol. 1994 Feb;104(2):431–438. [PMC free article] [PubMed]
  • Straub PF, Shen Q, Ho TD. Structure and promoter analysis of an ABA- and stress-regulated barley gene, HVA1. Plant Mol Biol. 1994 Oct;26(2):617–630. [PubMed]
  • Tarczynski MC, Jensen RG, Bohnert HJ. Stress protection of transgenic tobacco by production of the osmolyte mannitol. Science. 1993 Jan 22;259(5094):508–510. [PubMed]

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