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Plant Physiol. Jan 1996; 110(1): 171–178.
PMCID: PMC157706

Cloning of a second Arabidopsis peptide transport gene.

Abstract

Previously, we reported the isolation of a peptide transport gene designated AtPTR2 from Arabidopsis thaliana by functional complementation of a yeast peptide transport mutant. We now report the isolation of a second peptide transport gene (AtPTR2-B) from Arabidopsis using the same approach. Similar to the effects of transferring AtPTR2-A (previously called AtPTR2), transfer of AtPTR2-B to yeast peptide transport mutants restored the ability to grow on di- and tripeptides but not peptides four residues or longer. However, unlike yeast mutants complemented with either the yeast PTR2 gene or the AtPTR2-A gene, transformants expressing AtPTR2-B were only partially sensitive to toxic peptides. Northern analysis showed that AtPTR2-B was constitutively expressed in all plant organs. Studies of the kinetics indicated that AtPTR2-A and AtPTR2-B have Km values of 47 and 14 microM, respectively, with Vmax values of 0.061 and 0.013 nmol mg-1 cell dry weight s-1, respectively, when dileucine was used as a substrate. AtPTR2-B is encoded on a 2.0-kb cDNA corresponding to a 585-amino acid protein (64.4 kD). Hydropathy analysis indicates that the protein is highly hydrophobic and suggests that there are 12 putative transmembrane segments. AtPTR2-B, like AtPTR2-A, shares significant similarity to a number of other proteins involved in transport of peptides into cells.

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Selected References

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  • Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool. J Mol Biol. 1990 Oct 5;215(3):403–410. [PubMed]
  • Andreae WA, Good NE. The Formation of Indoleacetylaspartic Acid in Pea Seedlings. Plant Physiol. 1955 Jul;30(4):380–382. [PMC free article] [PubMed]
  • Basrai MA, Lubkowitz MA, Perry JR, Miller D, Krainer E, Naider F, Becker JM. Cloning of a Candida albicans peptide transport gene. Microbiology. 1995 May;141(Pt 5):1147–1156. [PubMed]
  • Daniel H, Adibi SA. Transport of beta-lactam antibiotics in kidney brush border membrane. Determinants of their affinity for the oligopeptide/H+ symporter. J Clin Invest. 1993 Nov;92(5):2215–2223. [PMC free article] [PubMed]
  • Dantzig AH, Hoskins JA, Tabas LB, Bright S, Shepard RL, Jenkins IL, Duckworth DC, Sportsman JR, Mackensen D, Rosteck PR, Jr, et al. Association of intestinal peptide transport with a protein related to the cadherin superfamily. Science. 1994 Apr 15;264(5157):430–433. [PubMed]
  • Fei YJ, Kanai Y, Nussberger S, Ganapathy V, Leibach FH, Romero MF, Singh SK, Boron WF, Hediger MA. Expression cloning of a mammalian proton-coupled oligopeptide transporter. Nature. 1994 Apr 7;368(6471):563–566. [PubMed]
  • Frommer WB, Hummel S, Rentsch D. Cloning of an Arabidopsis histidine transporting protein related to nitrate and peptide transporters. FEBS Lett. 1994 Jun 27;347(2-3):185–189. [PubMed]
  • Gietz D, St Jean A, Woods RA, Schiestl RH. Improved method for high efficiency transformation of intact yeast cells. Nucleic Acids Res. 1992 Mar 25;20(6):1425–1425. [PMC free article] [PubMed]
  • Hagting A, Kunji ER, Leenhouts KJ, Poolman B, Konings WN. The di- and tripeptide transport protein of Lactococcus lactis. A new type of bacterial peptide transporter. J Biol Chem. 1994 Apr 15;269(15):11391–11399. [PubMed]
  • Island MD, Naider F, Becker JM. Regulation of dipeptide transport in Saccharomyces cerevisiae by micromolar amino acid concentrations. J Bacteriol. 1987 May;169(5):2132–2136. [PMC free article] [PubMed]
  • Kyte J, Doolittle RF. A simple method for displaying the hydropathic character of a protein. J Mol Biol. 1982 May 5;157(1):105–132. [PubMed]
  • Lichliter WD, Naider F, Becker JM. Basis for the design of anticandidal agents from studies of peptide utilization in Canadida albicans. Antimicrob Agents Chemother. 1976 Sep;10(3):483–490. [PMC free article] [PubMed]
  • Minet M, Dufour ME, Lacroute F. Complementation of Saccharomyces cerevisiae auxotrophic mutants by Arabidopsis thaliana cDNAs. Plant J. 1992 May;2(3):417–422. [PubMed]
  • Naider F, Becker JM, Katzir-Katchalski E. Utilization of methionine-containing peptides and their derivatives by a methionine-requiring auxotroph of Saccharomyces cerevisiae. J Biol Chem. 1974 Jan 10;249(1):9–20. [PubMed]
  • Paulsen IT, Skurray RA. The POT family of transport proteins. Trends Biochem Sci. 1994 Oct;19(10):404–404. [PubMed]
  • Payne JW, Smith MW. Peptide transport by micro-organisms. Adv Microb Physiol. 1994;36:1–80. [PubMed]
  • Perry JR, Basrai MA, Steiner HY, Naider F, Becker JM. Isolation and characterization of a Saccharomyces cerevisiae peptide transport gene. Mol Cell Biol. 1994 Jan;14(1):104–115. [PMC free article] [PubMed]
  • Salmenkallio M, Sopanen T. Amino Acid and Peptide uptake in the scutella of germinating grains of barley, wheat, rice, and maize. Plant Physiol. 1989 Apr;89(4):1285–1291. [PMC free article] [PubMed]
  • Sopanen T, Burston D, Matthews DM. Uptake of small peptides by the scutellum of germinating barley. FEBS Lett. 1977 Jul 1;79(1):4–7. [PubMed]
  • Sopanen T, Burston D, Taylor E, Matthews DM. Uptake of glycylglycine by the scutellum of germinating barley grain. Plant Physiol. 1978 Apr;61(4):630–633. [PMC free article] [PubMed]
  • Steiner HY, Naider F, Becker JM. The PTR family: a new group of peptide transporters. Mol Microbiol. 1995 Jun;16(5):825–834. [PubMed]
  • Steiner HY, Song W, Zhang L, Naider F, Becker JM, Stacey G. An Arabidopsis peptide transporter is a member of a new class of membrane transport proteins. Plant Cell. 1994 Sep;6(9):1289–1299. [PMC free article] [PubMed]
  • Tiruppathi C, Ganapathy V, Leibach FH. Kinetic evidence for a common transporter for glycylsarcosine and phenylalanylprolylalanine in renal brush-border membrane vesicles. J Biol Chem. 1990 Sep 5;265(25):14870–14874. [PubMed]
  • Tsay YF, Schroeder JI, Feldmann KA, Crawford NM. The herbicide sensitivity gene CHL1 of Arabidopsis encodes a nitrate-inducible nitrate transporter. Cell. 1993 Mar 12;72(5):705–713. [PubMed]
  • Winter A, Thimann KV. Bound indoleacetic Acid in Avena coleoptiles. Plant Physiol. 1966 Feb;41(2):335–342. [PMC free article] [PubMed]

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