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Plant Physiol. Oct 1995; 109(2): 525–531.
PMCID: PMC157616

Polyphenol oxidase in potato. A multigene family that exhibits differential expression patterns.

Abstract

Polyphenol oxidase (PPO) activity in potato (Solanum tuberosum) plants was high in stolons, tubers, roots, and flowers but low in leaves and stems. PPO activity per tuber continued to increase throughout tuber development but was highest on a fresh weight basis in developing tubers. PPO activity was greatest at the tuber exterior, including the skin and cortex tissue 1 to 2 mm beneath the skin. Flowers had high PPO activity throughout development, particularly in the anthers and ovary. Five distinct cDNA clones encoding PPO were isolated from developing tuber RNA. POT32 was the major form expressed in tubers and was found in all parts of the tuber and at all stages of tuber development. It was also expressed in roots but not in photosynthetic tissues. POT33 was expressed in tubers but mainly in the tissue near the skin. POT72 was detected in roots and at low levels in developing tubers. NOR333 was identical with the P2 PPO clone previously isolated from potato leaves (M.D. Hunt, N.T. Eannetta, Y. Haifeng, S.M. Newman, J.C. Steffens [1993] Plant Mol Biol 21: 59-68) and was detected in young leaves and in tissue near the tuber skin but was highly expressed in flowers. The results indicate that PPO is present as a small multigene family in potato and that each gene has a specific temporal and spatial pattern of expression.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Boss PK, Gardner RC, Janssen BJ, Ross GS. An apple polyphenol oxidase cDNA is up-regulated in wounded tissues. Plant Mol Biol. 1995 Jan;27(2):429–433. [PubMed]
  • Cary JW, Lax AR, Flurkey WH. Cloning and characterization of cDNAs coding for Vicia faba polyphenol oxidase. Plant Mol Biol. 1992 Oct;20(2):245–253. [PubMed]
  • Delhaize E, Loneragan JF, Webb J. Development of three copper metalloenzymes in clover leaves. Plant Physiol. 1985 May;78(1):4–7. [PMC free article] [PubMed]
  • Hunt MD, Eannetta NT, Yu H, Newman SM, Steffens JC. cDNA cloning and expression of potato polyphenol oxidase. Plant Mol Biol. 1993 Jan;21(1):59–68. [PubMed]
  • Newman SM, Eannetta NT, Yu H, Prince JP, de Vicente MC, Tanksley SD, Steffens JC. Organisation of the tomato polyphenol oxidase gene family. Plant Mol Biol. 1993 Mar;21(6):1035–1051. [PubMed]
  • Robinson SP, Dry IB. Broad bean leaf polyphenol oxidase is a 60-kilodalton protein susceptible to proteolytic cleavage. Plant Physiol. 1992 May;99(1):317–323. [PMC free article] [PubMed]
  • Sanger F, Nicklen S, Coulson AR. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. [PMC free article] [PubMed]
  • Shahar T, Hennig N, Gutfinger T, Hareven D, Lifschitz E. The tomato 66.3-kD polyphenoloxidase gene: molecular identification and developmental expression. Plant Cell. 1992 Feb;4(2):135–147. [PMC free article] [PubMed]
  • Sommer A, Ne'eman E, Steffens JC, Mayer AM, Harel E. Import, targeting, and processing of a plant polyphenol oxidase. Plant Physiol. 1994 Aug;105(4):1301–1311. [PMC free article] [PubMed]
  • Vámos-Vigyázó L. Polyphenol oxidase and peroxidase in fruits and vegetables. Crit Rev Food Sci Nutr. 1981;15(1):49–127. [PubMed]

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