• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of plntphysLink to Publisher's site
Plant Physiol. Jul 1995; 108(3): 1277–1287.
PMCID: PMC157483

Elicitor-Induced Spruce Stress Lignin (Structural Similarity to Early Developmental Lignins).

Abstract

Suspension cultures of Picea abies (L.) Karst released polymeric material into the culture medium when treated with an elicitor preparation from the spruce needle pathogen Rhizosphaera kalkhoffii. The presence of lignin (about 35%, w/w) was demonstrated by phloroglucinol/HCI reactivity and quantitation with thioglycolic acid. Carbohydrate (about 14%, w/w) and protein (about 32%, w/w) were also detected. Amino acid analysis revealed that hydroxyproline and proline predominated. Thioacidolysis and subsequent Raney nickel desulfurization allowed the analysis of lignin-building units and interunit bonds. Compared with spruce wood lignin, an approximately 20-fold higher relative amount of p-hydroxyphenyl units was determined. A high content of p-hydroxyphenyl units is typical for certain developmental lignins, such as conifer compression wood and middle lamella lignins, as well as all induced cell culture lignins so far analyzed. Cross-linkages of the pinoresinol type ([beta]-[beta]) in the excreted cell culture lignin were markedly increased, whereas [beta]-1 interunit linkages were decreased relative to spruce wood lignin. The amount and nature of cross-linkages were shown to be intermediate between those in wood lignin and in enzymatically prepared lignins. In summary, the elicitor-induced stress lignin was excreted as a lignin-extensin complex that closely resembled early developmental lignins.

Full Text

The Full Text of this article is available as a PDF (1.0M).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Ayers AR, Ebel J, Valent B, Albersheim P. Host-Pathogen Interactions: X. Fractionation and Biological Activity of an Elicitor Isolated from the Mycelial Walls of Phytophthora megasperma var. sojae. Plant Physiol. 1976 May;57(5):760–765. [PMC free article] [PubMed]
  • Bradley DJ, Kjellbom P, Lamb CJ. Elicitor- and wound-induced oxidative cross-linking of a proline-rich plant cell wall protein: a novel, rapid defense response. Cell. 1992 Jul 10;70(1):21–30. [PubMed]
  • Bruce RJ, West CA. Elicitation of lignin biosynthesis and isoperoxidase activity by pectic fragments in suspension cultures of castor bean. Plant Physiol. 1989 Nov;91(3):889–897. [PMC free article] [PubMed]
  • Eberhardt TL, Bernards MA, He L, Davin LB, Wooten JB, Lewis NG. Lignification in cell suspension cultures of Pinus taeda. In situ characterization of a gymnosperm lignin. J Biol Chem. 1993 Oct 5;268(28):21088–21096. [PubMed]
  • Eulitz M, Murphy C, Weiss DT, Solomon A. Serologic and chemical differentiation of human lambda III light chain variable regions. J Immunol. 1991 May 1;146(9):3091–3096. [PubMed]
  • Iiyama K, Lam TBT, Stone BA. Covalent Cross-Links in the Cell Wall. Plant Physiol. 1994 Feb;104(2):315–320. [PMC free article] [PubMed]
  • Langebartels C, Kerner K, Leonardi S, Schraudner M, Trost M, Heller W, Sandermann H. Biochemical plant responses to ozone : I. Differential induction of polyamine and ethylene biosynthesis in tobacco. Plant Physiol. 1991 Mar;95(3):882–889. [PMC free article] [PubMed]
  • Lüderitz T, Schatz G, Grisebach H. Enzymic synthesis of lignin precursors. Purification and properties of 4-coumarate:CoA ligase from cambial sap of spruce (Picea abies L.). Eur J Biochem. 1982 Apr;123(3):583–586. [PubMed]
  • Sandermann H., Jr Higher plant metabolism of xenobiotics: the 'green liver' concept. Pharmacogenetics. 1994 Oct;4(5):225–241. [PubMed]
  • Showalter AM. Structure and function of plant cell wall proteins. Plant Cell. 1993 Jan;5(1):9–23. [PMC free article] [PubMed]

Articles from Plant Physiology are provided here courtesy of American Society of Plant Biologists

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...