Logo of brjpharmLink to Publisher's site
Br J Pharmacol. 2002 Jun; 136(3): 452–458.
PMCID: PMC1573364

‘Entourage' effects of N-acyl ethanolamines at human vanilloid receptors. Comparison of effects upon anandamide-induced vanilloid receptor activation and upon anandamide metabolism

Abstract

  1. The abilities of a series of saturated N-acyl ethanolamines and related compounds to affect the ability of anandamide (AEA) to produce a Ca2+ influx into human embryonic kidney cells expressing the human vanilloid receptor (hVR1-HEK293 cells) has been investigated.
  2. The C3:0, C4:0, C6:0 and C10:0 ethanolamides neither affected basal Ca2+-influx, nor the influx in response to a submaximal concentration of AEA (1 μM). In contrast, the C12:0, C17:0, C18:0 ethanolamides and the monounsaturated compound oleoylethanolamide (C18:1) greatly potentiated the response to AEA. Palmitoylethanolamide (C16:0) produced both a response per se and an augmentation of the response to AEA.
  3. Lauroylethanolamide (C12:0) produced a leftward shift in the dose-response curve for AEA. EC50 values for AEA to produce Ca2+ influx into hVR1-HEK293 cells were 1.8, 1.5, 1.1 and 0.22 μM in the presence of 0, 1, 3 and 10 μM lauroylethanolamide, respectively. Lauroylethanolamide did not affect the dose – response curves to capsaicin.
  4. Palmitoylethylamide was synthesized and found to be a mixed-type inhibitor (Ki(slope) 4.1 μM, Ki(intercept) 66 μM) of [3H]-AEA metabolism by rat brain membranes.
  5. The -amide, -ethylamide, -isopropylamide, -butylamide, -cyclohexamide and -trifluoromethyl ketone analogues of palmitoylethanolamide had little or no effect on the Ca2+ influx response to 1 μM AEA.
  6. There was no obvious relation between the abilities of the compounds to enhance the Ca2+ influx response to 1 μM AEA into hVR1-HEK293 cells and to prevent the hydrolysis of AEA by rat brain membranes.
  7. It is concluded that although palmitoylethanolamide has entourage-like effects at VR1 receptors expressed on hVR1-HEK293 cells, other N-acyl ethanolamines have even more dramatic potentiating effects. It is possible that they may play an important role under conditions where their synthesis is increased, such as in severe inflammation.
Keywords: Vanilloid receptors, anandamide, fatty acid amidohydrolase, N-acyl ethanolamines

Introduction

It is now well established that the N-acyl ethanolamine anandamide (arachidonoyl ethanolamide C20:4, AEA) not only acts as an endogenous ligand at cannabinoid receptors (Devane et al., 1992), but is also capable of activating vanilloid receptors (VR) (Zygmunt et al., 1999; Smart et al., 2000). In contrast to the activation produced by hydrogen ions (Caterina et al., 1997; McLatchie & Bevan, 2001), the VR agonist capsaicin is believed to activate VR1 receptors intracellularly (Jung et al., 1999; Welch et al., 2000), and a similar mechanism may exist for AEA, since blockade of AEA facilitated transport into hVR1-HEK cells transfected with VR1 receptors inhibits AEA-induced influx of calcium into the cell (De petrocellis et al., 2001a). Intracellular AEA is metabolized to arachidonic acid by the enzyme fatty acid amide hydrolase (FAAH) (Deutsch & Chin, 1993), and inhibition of the enzyme potentiates the effects of AEA upon VR1 receptors (de petrocellis et al., 2001a; Ross et al., 2001).

Whilst it is clear that AEA activates VR1 receptors, it is not as yet proven beyond doubt that AEA acts as an ‘endovanilloid' in the same way as it acts as an endocannabinoid (see Di marzo et al., 2001). The main obstacle to the acceptance of AEA as an endovanilloid has been its relatively low potency (Zygmunt et al., 1999; Smart et al., 2000), even after FAAH inhibition (Ross et al., 2001). However, there is evidence that the potency of AEA towards VR1 receptors can be regulated (di marzo et al., 2001). Thus, the naturally-occurring N-acyl ethanolamine palmitoylethanolamide (PEA, C16:0) potentiates the effects of AEA and resiniferatoxin at VR1 receptors (de petrocellis et al., 2001b). It is not known, however, whether these effects of PEA are shared by other N-acyl ethanolamines. In consequence, in the present study, the effects of a series of PEA analogues and homologues have been investigated with respect to their ability to potentiate the actions of AEA at VR1 receptors.

Methods

Compounds

Anandamide [ethanolamine-1-3H] ([3H]-AEA; specific activity 60 Ci mmol−1) was obtained from American Radiolabeled Chemicals, Inc. (St. Louis, MO, U.S.A.). The syntheses of propanoylethanolamide (C3:0), butanoylethanolamide (C4:0), hexanoylethanolamide (C6:0), octanoylethanolamide (C8:0), decanoylethanolamide (C10:0), lauroylethanolamide (C12:0), myristoylethanolamide (C14:0), palmitoylethanolamide (C16:0), palmitamide (C16:0), palmitoylbutylamide (C16:0), palmitoylisopropylamide (C16:0), palmitoylcyclohexamide (C16:0) and oleoylethanolamide (C18:1) have been described earlier (Lambert et al., 1999; 2001; Jonsson et al., 2001). Capsaicin and non-radioactive AEA (for the VR1 experiments) was purchased from Tocris (Bristol, U.K.). Heptadecanoylethanolamide (C17:0), stearoylethanolamide (C18:0), palmitoyl trifluoromethyl ketone and non-radioactive AEA (for the FAAH experiments) were purchased from Cayman Chemical Company (Ann Arbor, MI, U.S.A.). Fatty acid free bovine serum albumin was obtained from the Sigma Chemical Co. (St Louis, MO, U.S.A.). Cell culture media were obtained from Gibco/Life Technologies.

Synthesis of palmitoylethylamide (PA-Et)

In a two-neck flask, 4.17 g of ethylamine (92.6 mmoles) were poured into 20 ml of dry methylene chloride. The solution was cooled in an ice bath and magnetically stirred. Palmitoyl chloride (2.74 g, 10 mmoles) was added dropwise. The reaction mixture was stirred for 12 h at room temperature and then washed with 5% sodium bicarbonate solution, HCl 1 M and brine. The organic layer was dried (MgSO4) and the solvent evaporated under reduced pressure to give 2.34 g (83%) of a white solid.

m.p.: 62 – 63°C (uncorrected); TLC (chloroform : methanol 6 : 4 v v−1) Rf=0.86; spectroscopic data: 1H NMR (d-CDCl3) δ (p.p.m.) 0.86 (t, J=7 Hz, 3 H); 1.14 (t, J=7 Hz, 3 H); 1.16 – 1.24 (m, 26 H); 2.1 (t, J=7 Hz, 2 H); 3.2 – 3.33 (m, 2 H); 5.4 (NH); 13C NMR (d-CDCl3) δ (p.p.m.) 14.1; 14.95 (CH3); 22.7; 25.8; 29.26; 29.37; 29.5; 29.63; 29.64; 29.65; 29.66; 29.68; 29.69 ; 29.8; 31.94; 34.3; 36.94 (CH2); 172.97 (C=O); mass spectrometry [M+]=283; IR v (cm−1): 3298 (NH); 1633 (C=O).

Cell culture

hVR1-HEK293 cells were grown as monolayers in minimum essential medium (MEM) supplemented with non-essential amino acids, 10% foetal calf serum, and 0.2 mM L-glutamine, and maintained under 95%/5% O2/CO2 at 37°C. Cells were passaged every 3 – 4 days and the highest passage number used was 27.

Measurement of [Ca2+]i using the FLIPR technology

The method of Smart et al. (2000) was used. hVR1-HEK293 cells were seeded (density 25×103 cells well−1) into black walled clear-base 96-well plates (Costar, U.K.) and cultured overnight in MEM, supplemented as described above. The cells were then incubated at 25°C for 120 min with MEM containing the cytoplasmic calcium indicator, Fluo-3AM (4 μM; Teflabs, Austin, TX, U.S.A.). After washing the plates four times with Tyrode's medium containing 0.1% bovine serum albumin, they were placed into a FLIPR (Molecular Devices, U.K.) to monitor cell fluorescence (λEX=488 nm, λEM=540 nm) before and after the addition of various compounds in the presence or absence of 1 μM AEA. Incubations were routinely run over 3 min, and the responses generally appeared within a few seconds of injection of the anandamide, peaking at about 30 s. Responses were measured as peak fluorescence intensity (FI) minus basal FI, and are expressed as a percentage of the response obtained with either anandamide or capsaicin, as appropriate.

Assay of FAAH activity

The assay used was that of Omeir et al. (1995) adapted to the tritiated substrate (Fowler et al., 1997). Briefly, frozen brains (minus cerebellum) from adult rats that had been stored at −70°C were thawed and homogenized at 4°C in 20 mM HEPES buffer, pH 7.0, with 1 mM MgCl2. The homogenates were centrifuged twice at 36,000×g for 20 min at 4°C. Tissue pellets were resuspended in homogenization buffer and incubated at 37°C for 15 min, after which they were centrifuged at 36,000×g for 20 min at 4°C. Membranes were re-suspended in 50 mM Tris-HCl buffer, pH 7.4, containing 1 mM EDTA and 3 mM MgCl2, and stored at −70°C until used for assay. Upon assay, membranes [12.5 μg protein assay−1], palmitoylethylamide (PA-Et) or ethanol carrier, [3H]-AEA and assay buffer (10 mM Tris-HCl, 1 mM EDTA, 1% (w v−1) fatty acid-free bovine serum albumin, pH 7.6) (final assay volume of 200 μl) were incubated for 10 min at 37°C. Reactions were stopped by placing the tubes in ice and thereafter adding 400 μl of chloroform : methanol (1 : 1 v v−1). Phases were separated by centrifugation in a bench centrifuge after the samples had been thoroughly mixed. Aliquots (200 μl) of the methanol/buffer phase containing the [3H]-ethanolamine product were removed and analysed for radioactivity by liquid scintillation spectroscopy with quench correction. Blanks contained distilled water instead of the membranes.

Calculation of EC50, Ki(slope) and Ki(intercept) values

EC50 values were calculated from the mean values at each AEA or capsaicin concentration using the built-in equation ‘sigmoid dose-response (variable slope)' of the GraphPad Prism computer programme (GraphPad Software Inc., San Diego, CA, U.S.A.). Top and bottom values were set to 100 and 0, respectively. Apparent KM and Vmax values for inhibition of [3H]-AEA metabolism by PA-Et were calculated from the mean data using the Direct Linear Plot analysis (Eisenthal & Cornish-Bowden, 1974) and the Enzyme Kinetics v 1.4 software package (Trinity Software, Campton, NH, U.S.A.). These values were used in secondary replots of [PA-Et] vs KMapp/Vmax app and 1/Vmax app to determine Ki(slope) and Ki(intercept) values, respectively.

Results

Effect of N-acylethanolamines upon AEA-induced activation of VR1 receptors in hVR1-HEK293 cells

A series of N-acylethanolamines ranging from 3 carbons (propanoylethanolamide) up to 18 carbons (stearoylethanolamide [C18:0], oleoylethanolamide (OEA, [C18:1]) were investigated (Figure 1). The compounds were tested at a concentration of 10 μM for effects per se, and for their ability to affect the Ca2+-influx response produced by a submaximal concentration of AEA (1 μM). Two series of experiments were undertaken, with AEA, PEA and OEA being tested in both experiments. Very similar values were seen for these compounds in both sets of experiments. For simplicity, the second of these values are shown in the figures.

Figure 1
(a) Effects of a series of N-acyl ethanolamines upon the Ca2+-influx in hVR1-HEK293 cells in the absence and presence of 1 μM AEA. The compounds were used at a concentration of 10 μM. Shown are means±s.e.mean ...

With the exception of PEA, none of the N-acyl ethanolamines produced a Ca2+-response per se in the hVR1-HEK293 cells (Figure 1a). The C3:0, C4:0, C6:0 and C10:0 compounds were also without effect upon the response to AEA. In contrast, the response to AEA was greatly potentiated by the C12:0, C17:0, C18:0 and C18:1 compounds, so that in the case of the C12:0 compound (lauroylethanolamine), the response to 1 μM AEA became maximal (Figure 1a). In Figure 1b, the ability of the compounds to inhibit the FAAH-catalyzed metabolism of AEA is shown. There was no obvious relation between the abilities of the compounds to inhibit AEA metabolism and to potentiate the VR1-mediated Ca2+ influx in the hVR1-HEK293 cells. Thus, for example, the C18:0 and C18:1 compounds potentiate the Ca2+ influx response to AEA to about the same extent, but have greatly differing abilities to inhibit [3H]-AEA metabolism. Conversely, the C12:0 and C14:0 compounds produce rather similar inhibitions of [3H]-AEA metabolism, but differ greatly in their ability to potentiate the Ca2+ influx response to AEA (Figure 1a, b).

Comparison of the effects of lauroylethanolamide (C12:0) upon the Ca2+ influx response to AEA and capsaicin in hVR1-HEK293 cells

Dose-response curves for the stimulation of Ca2+ influx in hVR1-HEK293 cells by AEA and capsaicin are shown in Figure 2. Lauroylethanolamide produced a leftward shift in the AEA dose-response curve, so that the potency of AEA was increased approximately 10 fold at the highest lauroylethanolamide concentration tested (10 μM) (Figure 2a). Thus, the EC50 values for AEA calculated from the curves were 1.8, 1.5, 1.1 and 0.22 μM in the presence of 0, 1, 3 and 10 μM lauroylethanolamide, respectively. In contrast to the effects on AEA, lauroylethanolamide did not affect the dose – response curves to capsaicin, with EC50 values in the range of 10 – 13 nM being found for the four curves (Figure 2b).

Figure 2
Effects of lauroylethanolamide (C12:0) upon the the Ca2+-influx in hVR1-HEK293 cells produced by (a) AEA and (b) capsaicin. Data are means of three experiments. In (a) the s.e.means are shown (when not enclosed by the symbols) for the sake of ...

Effects of PEA analogues upon FAAH activity and the Ca2+ influx response to AEA and capsaicin in hVR1-HEK293 cells

Six analogues to PEA where the ethanolamine groupwas replaced by -amide (PA-NH2), -ethylamide (PA-Et), -isopropylamide (PIA), -butylamide (PBA), -cyclohexamide (PCA) and -trifluoromethyl ketone (PTMK) groups were investigated. Of these, no data is available on the ability of PA-Et to interact with FAAH, so the effects of this compound upon [3H]-AEA by rat brain membranes was determined. PA-Et was found to be a mixed-type inhibitor, with a Ki(slope) value of 4.1 μM and a Ki(intercept) value of 66 μM (Figure 3).

Figure 3
Mode of inhibition of [3H]-AEA metabolism in rat brain membranes by palmitoylethylamide (PA-Et). Means±s.e.mean of three experiments are shown in the main plot. A secondary Lineweaver – Burk replot of the ...

In Figure 4, the effects of the six PEA analogues upon basal and AEA-induced Ca2+ influx in hVR1-HEK293 cells are shown together with data on the abilities of the compounds to affect [3H]-AEA metabolism. The experiments were conducted concomitantly with the experiments shown in Figure 1, so the data for AEA is the same in both figures. None of the compounds produced marked effects either per se, or upon the Ca2+ influx response to 1 μM AEA (Figure 4a), despite a large variation in the abilities of the compounds to prevent [3H]-AEA metabolism (Figure 4b).

Figure 4
(a) Effects of a series of palmitoylethanolamide analogues upon the Ca2+-influx in hVR1-HEK293 cells in the absence and presence of 1 μM AEA. Shown are means±s.e.mean of three experiments. The concentrations of the palmitoylethanolamide ...

Discussion

In the short time since the publication by Zygmunt et al. (1999) that AEA was able to activate VR1 receptors, a number of studies, not the least in this journal, have demonstrated the involvement of these sensory nerve receptors in the pharmacological effects of exogenously applied AEA in nociceptive (Gauldie et al., 2001; Tognetto et al., 2001) and pulmonary (Craib et al., 2001; Smith & McQueen, 2001) function as a consequence of its effects on sensory neurotransmission (Zygmunt et al., 1999; Ralevic et al., 2001; Ross et al., 2001; Mang et al., 2001). However, it is still a matter of debate as to whether AEA acts as an endogenous vanilloid (see di marzo et al., 2001).

In a recent study, it was reported that the N-acylethanolamine palmitoylethanolamide (C16:0) was able to increase the VR1-mediated Ca2+ influx in response to AEA in hVR1-HEK293 cells (de petrocellis et al., 2001b). These authors found that PEA produced a 2 fold decrease in the EC50 value for AEA and was also able to potentiate the responses to the VR1 stimulators capsaicin and resiniferatoxin, although the stimulation of the response to resiniferatoxin was more marked than for capsaicin. At a concentration of 1 μM AEA (the concentration used in the present study), PEA was without effect upon the response to AEA, whereas a potentiation was seen at AEA concentrations <250 nM (de petrocellis et al., 2001b). The apparent potency of AEA is highly dependent upon the assay conditions, in particular upon the concentration of bovine serum albumin (de petrocellis et al., 2001b) (which is required in the FLIPR assay to prevent binding of the AEA to the plastic wells). However, the AEA concentration used here (1 μM) relative to its EC50 value (1.8 μM) corresponds to a concentration of ∼240 nM under the conditions used by de petrocellis et al. (2001b) relative to their EC50 value of 0.44 μM. In addition to its effects on AEA-induced Ca2+-influx, the authors found that PEA produced a 5 fold decrease in the Ki for the inhibition of [3H]-resinaferatoxin binding by AEA (de petrocellis et al., 2001b). The authors suggested that this action of PEA could be described as an ‘entourage' effect, similar to that seen for the ability of the 2-arachidonoyl glycerol homologues 2-palmitoylglycerol and 2-linoleoyl-glycerol to potentiate the cannabinoid receptor-mediated effects of 2-arachidonoyl glycerol without themselves having affinities for cannabinoid receptors (Ben-Shabat et al., 1998).

In the present study, we have investigated a series of palmitoylethanolamide homologues and analogues in order to investigate whether such an entourage effect is unique for PEA, or whether other N-acylethanolamines produce even more dramatic effects. In addition, in view of the finding that PEA acts as a partial agonist at VR1-receptors overexpressed in hVR1-HEK293 cells (Smart et al., 2000), effects of the compounds per se upon VR1-receptors were investigated. PEA was found to produce effects both per se and upon the response to AEA, essentially in agreement with the study of de petrocellis et al. (2001b). These effects are lost when the -ethanolamine group was replaced by -ethylamine (PA-Et), -isopropylamine (PIA), -butylamine (PBA), -cyclohexamine (PCA) and -trifluoromethyl ketone (PTMK) groups, suggesting a degree of specificity in the entourage actions of PEA, although it should be pointed out as a caveat that the modest inhibitory effects of PIA and PCA on the transport of AEA, at least in rodent cell lines (Jonsson et al., 2001), may mask small augmentations of the response to AEA, given that in hVR1-HEK cells AEA uptake inhibitors block the Ca2+-response to this compound (de petrocellis et al., 2001a). The finding that palmitamide (PA-NH2) has at best modest effects in this assay is important. This compound is a good substrate of FAAH (Boger et al., 2000), and its low activity would suggest that the effects of PEA are not a result of its metabolism to the free fatty acid.

PEA, however, was not the best entourage compound at the assay concentration of AEA used, since lauroylethanolamide (C12:0), heptadecanoylethanolamide (C17:0), stearoylethanolamide (C18:0) and oleoylethanolamide (C18:1) produced much more robust potentiations of the effect of AEA without producing Ca2+-influxes in the cells in its absence. The C12:0 compound potentiated the effects of AEA but not of capsaicin, suggesting that in some way it increases the affinity of the VR1 receptor for the endogenous compound but not the exogenous compound. At first sight, this differs from the observations with PEA (de petrocellis et al., 2001b), and might suggest that the C12:0 and C16:0 compounds may exert their effects in different ways. However, the effect of PEA upon the response to capsaicin reported by de petrocellis et al. (2001b) was rather modest.

It has long been known that N-acyl ethanolamines are substrates for amidohydrolase activities that are presumed to be identical to fatty acid amide hydrolase (FAAH, Epps et al., 1982; Schmid et al., 1985, Deutsch & Chin, 1993), and that they will thus compete with AEA for this enzyme (Jonsson et al., 2001). In theory, such a potentiation of the response to AEA, but not capsaicin, could be a result of competition for FAAH, so that more AEA is available for interaction with the VR1 receptor. Certainly, the FAAH inhibitors phenylsulphonyl methyl fluoride and methyl arachidonoyl fluorophosphonate potentiate the Ca2+-response to a low concentration (100 nM) of AEA but not capsaicin (10 nM) in hVR1-HEK cells (de petrocellis et al., 2001a). However, in that study, the FAAH inhibitors were preincubated with the cells for 10 min prior to addition of the agonists, whereas in the present study, they were added concomitantly. Given the very short incubation times used in the FLIPR methodology (the peak response to AEA occurs at about 30 s after administration, Smart et al., 2000), together with the finding by de petrocellis et al. (2001b), that PEA at the concentrations used did not affect [14C]-AEA hydrolysis by hVR1-HEK293 cell membranes, it is unlikely that FAAH inhibition is involved in the actions of the NAEs. Consistent with this conclusion, the present data show no relationship between the ability of the compounds tested to inhibit [3H]-AEA metabolism and their ability to potentiate the VR1-mediated actions of AEA. The best illustrations of this are PTMK, stearoylethanolamide and OEA. PTMK produces an almost complete inhibition of FAAH but does not affect the VR1 response to AEA; Stearoylethanolamide and OEA produce similar robust augmentations of the VR1 response to AEA despite large differences in their abilities to affect AEA metabolism. Of course, it should be mentioned as a caveat that the FAAH data was obtained using rat brain membranes, whereas the HEK cells are of human origin. However, rat and human FAAH have very similar sequences (82% amino acid identity and only one divergent amino acid in the amidase signature sequence) and are able to hydrolyze a series of N-acyl amides with rather similar relative specificities (Cravatt et al., 1996; Giang & Cravatt, 1997). Thus, in agreement with the conclusion of de petrocellis et al. (2001b), FAAH inhibition does not account for the potentiation of AEA effects upon Ca2+-influx in hVR1-HEK293 cells produced by the N-acyl ethanolamines.

In conclusion, the present study has confirmed that PEA has entourage-like effects at VR1 receptors expressed on hVR1-HEK293 cells and demonstrated that other N-acyl ethanolamines have even more dramatic potentiating effects. It is of course hazardous to extrapolate data obtained from transfected cells to the situation in vivo. However, one important property of N-acyl ethanolamines in the body is that their synthesis is dramatically increased under certain conditions of cellular stress, ischaemia and inflammation (see e.g. Epps et al., 1979; Kondo et al., 1998; Berdyshev et al., 2000; Hansen et al., 2001). Thus, for example, 9 h following cadmium chloride-induced inflammation in the rat testis, the levels of the C16:0 and C18:0 N-acyl ethanolamines were increased 39 and 21 fold (Kondo et al., 1998). Although there is no apparent increased sensitivity of peripheral nociceptors to exogenously applied AEA in arthritic rat knee joints (Gauldie et al., 2001), it is nevertheless tempting to speculate that N-acyl ethanolamines may be an important regulator of the actions of AEA upon vanilloid receptors under severe inflammatory conditions.

Acknowledgments

This study was supported by grants from the Swedish Medical Research Foundation (Grant no. 12158), the Swedish Asthma- and Allergy Association's Research Foundation, Konung Gustav V's and Drottning Victorias Foundation, the Swedish Psoriasis Association, and the Research Funds of the Medical Odontological Faculty, Umeå University. The chemical work was financially supported by the Belgian National Fund for Scientific Research and a FSR grant from the Université catholique de Louvain.

Abbreviations

AEAanandamide
FAAHfatty acid amidohydrolase
MEMminimal essential medium
OEAoleoylethanolamide
PA-Etpalmitoylethylamide
PA-NH2palmitamide
PBApalmitoylbutylamide
PCApalmitoylcyclohexamide
PEApalmitoylethanolamide
PIApalmitoylisopropylamide
PTMKpalmitoyl trifluoromethyl ketone
VRvanilloid receptors

References

  • BEN-SHABAT S., FRIDE E., SHESKIN T., TAMIRI T., RHEE M.-H., VOGEL Z., BISOGNO T., DE PETROCELLIS L., DI MARZO V., MECHOULAM R. An entourage effect: inactive endogenous fatty acid glycerol esters enhance 2-arachidonyl-glycerol cannabinoid activity. Eur. J. Pharmacol. 1998;353:23–31. [PubMed]
  • BERDYSHEV E.V., SCHMID P.C., DONG Z., SCHMID H.H.O. Stress-induced generation of N-acylethanolamines in mouse epidermal JB6 P+ cells. Biochem. J. 2000;346:369–374. [PMC free article] [PubMed]
  • BOGER D.L., FECIK R.A., PATTERSON J.E., MIYAUCHI H., PATRICELLI M.P., CRAVATT B.F. Fatty acid amide hydrolase substrate specificity. Bioorg. Med. Chem. Lett. 2000;10:2613–2616. [PubMed]
  • CATERINA M.J., SCHUMACHER M.A., TOMINAGA M., ROSEN T.A., LEVINE J.D., JULIUS D. The capsaicin receptor: a heat-activated ion channel in the pain pathway. Nature. 1997;389:816–824. [PubMed]
  • CRAIB S.J., ELLINGTON H.C., PERTWEE R.G., ROSS R.A. A possible role of lipoxygenase in the activation of vanilloid receptors by anandamide in the guinea-pig bronchus. Br. J. Pharmacol. 2001;134:30–37. [PMC free article] [PubMed]
  • CRAVATT B.F., GIANG D.K., MAYFIELD S.P., BOGER D.L., LERNER R.A., GILULA N.B. Molecular characterization of an enzyme that degrades neuromodulatory fatty-acid amides. Nature. 1996;384:83–87. [PubMed]
  • DE PETROCELLIS L., BISOGNO T., MACCARRONE M., DAVIS J.B., FINAZZI-AGRÓ A., DI MARZO V. The activity of anandamide at vanilloid VR1 receptors requires facilitated transport across the cell membrane and is limited by intracellular metabolism. J. Biol. Chem. 2001a;276:12856–12863. [PubMed]
  • DE PETROCELLIS L., DAVIS J.B., DI MARZO V. Palmitoylethanolamide enhances anandamide stimulation of human vanilloid VR1 receptors. FEBS Letts. 2001b;506:253–256. [PubMed]
  • DEVANE W.A., HANUS L., BREUER A., PERTWEE R.G., STEVENSON L.A., GRIFFIN G., GIBSON D., MANDELBAUM A., ETINGER A., MECHOULAM R. Isolation and structure of a brain constituent that binds to the cannabinoid receptor. Science. 1992;258:1946–1949. [PubMed]
  • DEUTSCH D.G., CHIN S.A. Enzymatic synthesis and degradation of anandamide, a cannabinoid receptor agonist. Biochem. Pharmacol. 1993;46:791–796. [PubMed]
  • DI MARZO V., BISOGNO T., DE PETROCELLIS L. Anandamide: some like it hot. Trends Pharmacol. Sci. 2001;22:346–349. [PubMed]
  • EISENTHAL R., CORNISH-BOWDEN A. The direct linear plot. A new graphical procedure for estimating enzyme kinetic parameters. Biochem. J. 1974;139:715–720. [PMC free article] [PubMed]
  • EPPS D.E., PALMER J.W., SCHMID H.H.O., PFEIFFER D.R. Inhibition of permeability-dependent Ca2+ release from mitochondria by N-acylethanolamines, a class of lipids synthesized in ischemic heart tissue. J. Biol. Chem. 1982;257:1383–1391. [PubMed]
  • EPPS D.E., SCHMID P.C., NATARAJAN V., SCHMID H.H.O. N-Acylethanolamine accumulation in infarcted myocardium. Biochem. Biophys. Res. Commun. 1979;90:628–633. [PubMed]
  • FOWLER C.J., TIGER G., STENSTRÖM A. Ibuprofen inhibits rat brain deamidation of anandamide at pharmacologically relevant concentrations. Mode of inhibition and structure-activity relationship. J. Pharmacol. Exp. Ther. 1997;283:729–734. [PubMed]
  • GIANG D.K., CRAVATT B.F. Molecular characterization of human and mouse fatty acid amide hydrolases. Proc. Natl. Acad. Sci. USA. 1997;94:2238–2242. [PMC free article] [PubMed]
  • GAULDIE S.D., MCQUEEN D.S., PERTWEE R., CHESSELL I.P. Anandamide activates peripheral nociceptors in normal and arthritic knee joints. Br. J. Pharmacol. 2001;132:617–621. [PMC free article] [PubMed]
  • HANSEN H.H., SCHMID P.C., BITTIGAU P., LASTRES-BECKER I., BERRENDERO F., MANZANARES J., IKONOMIDOU C., SCHMID H.H., FERNÁNDEZ-RUIZ J.J., HANSEN H.S. Anandamide, but not 2-arachidonoylglycerol, accumulates during in vivo neurodegeneration. J. Neurochem. 2001;78:1415–1427. [PubMed]
  • JONSSON K.-O., VANDEVOORDE S., LAMBERT D.M., TIGER G., FOWLER C.J. Effects of homologues and analogues of palmitoylethanolamide upon the inactivation of the endocannabinoid anandamide. Br. J. Pharmacol. 2001;133:1263–1275. [PMC free article] [PubMed]
  • JUNG J., HWANG S.W., KWAK J., LEE S.-Y., KANG C.-J., KIM W.B., KIM D., OH U. Capsaicin binds to the intracellular domain of the capsaicin-activated ion channel. J. Neurosci. 1999;19:529–538. [PubMed]
  • KONDO S., SUGIURA T., KODAKA T., KUDO N., WAKU K., TOKUMURA A. Accumulation of various N-acylethanolamines including N-arachidonoylethanolamine (anandamide) in cadmium chloride-administered rat testis. Arch. Biochem. Biophys. 1998;354:303–310. [PubMed]
  • LAMBERT D.M., DIPAOLO F.G., SONVEAUX P., KANYONYO M., GOVAERTS S.J., HERMANS E., BUEB J., DELZENNE N.M., TSCHIRHART E.J. Analogues and homologues of N-palmitoylethanolamide, a putative endogenous CB2 cannabinoid, as potential ligands for the cannabinoid receptors. Biochim. Biophys. Acta. 1999;1440:266–274. [PubMed]
  • LAMBERT D.M., VANDEVOORDE S., DIEPENDAELE G., GOVAERTS S.J., ROBERT A.R. Anticonvulsant activity of N-palmitoylethanolamide, a putative endocannabinoid, in mice. Epilepsia. 2001;42:321–327. [PubMed]
  • MANG C.F., ERBELDING D., KILBINGER H. Differential effects of anandamide on acetylcholine release in the guinea-pig ileum mediated via vanilloid and non-CB1 cannabinoid receptors. Br. J. Pharmacol. 2001;134:161–167. [PMC free article] [PubMed]
  • MCLATCHIE L.M., BEVAN S. The effects of pH on the interaction between capsaicin and the vanilloid receptor in rat dorsal root ganglia neurons. Br. J. Pharmacol. 2001;132:899–908. [PMC free article] [PubMed]
  • OMEIR R.L., CHIN S., HONG Y., AHERN D.G., DEUTSCH D.G. Arachidonoyl ethanolamide-[1,2-14C] as a substrate for anandamide amidase. Life Sci. 1995;56:1999–2005. [PubMed]
  • RALEVIC V., KENDALL D.A., JERMAN J.C., MIDDLEMISS D.N., SMART D. Cannabinoid activation of recombinant and endogenous vanilloid receptors. Eur. J. Pharmacol. 2001;424:211–219. [PubMed]
  • ROSS R.A., GIBSON T.M., BROCKIE H.C., LESLIE M., PASHMI G., CRAIB S.J., DI MARZO V., PERTWEE R.G. Structure-activity relationship for the endogenous cannabinoid, anandamide and certain of its analogues at vanilloid receptors in transfected cells and vas deferens. Br. J. Pharmacol. 2001;132:631–640. [PMC free article] [PubMed]
  • SCHMID P.C., ZUZARTE-AUGUSTIN M.L., SCHMID H.H.O. Properties of rat liver N-acylethanolamine amidohydrolase. J. Biol. Chem. 1985;260:14145–14149. [PubMed]
  • SMART D., GUNTHORPE M.J., JERMAN J.C., NASIR S., GRAY J., MUIR A.I., CHAMBERS J.K., RANDALL A.D., DAVIS J.B. The endogenous lipid anandamide is a full agonist at human vanilloid receptor (hVR1) Br. J. Pharmacol. 2000;129:227–230. [PMC free article] [PubMed]
  • SMITH P.J.W., MCQUEEN D.S. Anandamide induces cardiovascular and respiratory reflexes via vasosensory nerves in the anaesthetized rat. Br. J. Pharmacol. 2001;134:655–663. [PMC free article] [PubMed]
  • TOGNETTO M., AMADESI S., HARRISON S., CREMINON C., TREVISANI M., CARRERAS M., MATERA M., GEPPETTI P., BIANCHI A. Anandamide excites central terminals of dorsal root ganglion neurons via vanilloid receptor-1 activation. J. Neurosci. 2001;21:1104–1109. [PubMed]
  • WELCH J.M., SIMON S.A., REINHART P.H. The activation mechanism of rat vanilloid receptor 1 by capsaicin involves the pore domain and differs from the activation by either acid or heat. Proc. Natl. Acad. Sci. USA. 2000;97:13889–13894. [PMC free article] [PubMed]
  • ZYGMUNT P.M., PETERSSON J., ANDERSSON D.A., CHUANG H-H., SØRGÅRD M., DI MARZO V., JULIUS D., HÖGESTÄTT E.D. Vanilloid receptors on sensory nerves mediate the vasodilator action of anandamide. Nature. 1999;400:452–457. [PubMed]

Articles from British Journal of Pharmacology are provided here courtesy of The British Pharmacological Society
PubReader format: click here to try

Formats:

Save items

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

  • Compound
    Compound
    PubChem Compound links
  • MedGen
    MedGen
    Related information in MedGen
  • PubMed
    PubMed
    PubMed citations for these articles
  • Substance
    Substance
    PubChem Substance links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...