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Plant Cell. 1997 Nov; 9(11): 1963–1971.
PMCID: PMC157050

Aux/IAA proteins repress expression of reporter genes containing natural and highly active synthetic auxin response elements.

Abstract

A highly active synthetic auxin response element (AuxRE), referred to as DR5, was created by performing site-directed mutations in a natural composite AuxRE found in the soybean GH3 promoter. DR5 consisted of tandem direct repeats of 11 bp that included the auxin-responsive TGTCTC element. The DR5 AuxRE showed greater auxin responsiveness than a natural composite AuxRE and the GH3 promoter when assayed by transient expression in carrot protoplasts or in stably transformed Arabidopsis seedlings, and it provides a useful reporter gene for studying auxin-responsive transcription in wild-type plants and mutants. An auxin response transcription factor, ARF1, bound with specificity to the DR5 AuxRE in vitro and interacted with Aux/IAA proteins in a yeast two-hybrid system. Cotransfection experiments with natural and synthetic AuxRE reporter genes and effector genes encoding Aux/IAA proteins showed that overexpression of Aux/IAA proteins in carrot protoplasts resulted in specific repression of TGTCTC AuxRE reporter gene expression.

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Selected References

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  • Abel S, Theologis A. Early genes and auxin action. Plant Physiol. 1996 May;111(1):9–17. [PMC free article] [PubMed]
  • Abel S, Oeller PW, Theologis A. Early auxin-induced genes encode short-lived nuclear proteins. Proc Natl Acad Sci U S A. 1994 Jan 4;91(1):326–330. [PMC free article] [PubMed]
  • Abel S, Nguyen MD, Theologis A. The PS-IAA4/5-like family of early auxin-inducible mRNAs in Arabidopsis thaliana. J Mol Biol. 1995 Aug 25;251(4):533–549. [PubMed]
  • Ainley WM, Walker JC, Nagao RT, Key JL. Sequence and characterization of two auxin-regulated genes from soybean. J Biol Chem. 1988 Aug 5;263(22):10658–10666. [PubMed]
  • Bevan M. Binary Agrobacterium vectors for plant transformation. Nucleic Acids Res. 1984 Nov 26;12(22):8711–8721. [PMC free article] [PubMed]
  • Durfee T, Becherer K, Chen PL, Yeh SH, Yang Y, Kilburn AE, Lee WH, Elledge SJ. The retinoblastoma protein associates with the protein phosphatase type 1 catalytic subunit. Genes Dev. 1993 Apr;7(4):555–569. [PubMed]
  • Hagen G, Martin G, Li Y, Guilfoyle TJ. Auxin-induced expression of the soybean GH3 promoter in transgenic tobacco plants. Plant Mol Biol. 1991 Sep;17(3):567–579. [PubMed]
  • Leyser HM, Lincoln CA, Timpte C, Lammer D, Turner J, Estelle M. Arabidopsis auxin-resistance gene AXR1 encodes a protein related to ubiquitin-activating enzyme E1. Nature. 1993 Jul 8;364(6433):161–164. [PubMed]
  • Liu ZB, Ulmasov T, Shi X, Hagen G, Guilfoyle TJ. Soybean GH3 promoter contains multiple auxin-inducible elements. Plant Cell. 1994 May;6(5):645–657. [PMC free article] [PubMed]
  • McClure BA, Hagen G, Brown CS, Gee MA, Guilfoyle TJ. Transcription, organization, and sequence of an auxin-regulated gene cluster in soybean. Plant Cell. 1989 Feb;1(2):229–239. [PMC free article] [PubMed]
  • Oeller PW, Keller JA, Parks JE, Silbert JE, Theologis A. Structural characterization of the early indoleacetic acid-inducible genes, PS-IAA4/5 and PS-IAA6, of pea (Pisum sativum L.). J Mol Biol. 1993 Oct 20;233(4):789–798. [PubMed]

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