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Plant Cell. 1997 September; 9(9): 1515–1525. | PMCID: PMC157030 |
Expression of the C4 Me1 Gene from Flaveria bidentis Requires an Interaction between 5[prime] and 3[prime] Sequences. JS Marshall, JD Stubbs, JA Chitty, B Surin, and WC Taylor CSIRO Plant Industry, GPO Box 1600, Canberra 2601, Australia. The efficient functioning of C4 photosynthesis requires the strict compartmentation of a suite of enzymes in either mesophyll or bundle sheath cells. To determine the mechanism controlling bundle sheath cell-specific expression of the NADP-malic enzyme, we made a set of chimeric constructs using the 5[prime] and 3[prime] regions of the Flaveria bidentis Me1 gene fused to the [beta]-glucuronidase gusA reporter gene. The pattern of GUS activity in stably transformed F. bidentis plants was analyzed by histochemical and cell separation techniques. We conclude that the 5[prime] region of Me1 determines bundle sheath specificity, whereas the 3[prime] region contains an apparent enhancer-like element that confers high-level expression in leaves. The interaction of 5[prime] and 3[prime] sequences was dependent on factors that are present in the C4 plant but not found in tobacco. The Full Text of this article is available as a PDF (1.6M). These references are in PubMed. This may not be the complete list of references from this article. - Bansal KC, Bogorad L. Cell type-preferred expression of maize cab-m1: repression in bundle sheath cells and enhancement in mesophyll cells. Proc Natl Acad Sci U S A. 1993 May 1;90(9):4057–4061. [PubMed]
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