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Plant Cell. 1997 Aug; 9(8): 1369–1380.
PMCID: PMC157004

Chlamydomonas Xanthophyll Cycle Mutants Identified by Video Imaging of Chlorophyll Fluorescence Quenching.


The photosynthetic apparatus in plants is protected against oxidative damage by processes that dissipate excess absorbed light energy as heat within the light-harvesting complexes. This dissipation of excitation energy is measured as nonphotochemical quenching of chlorophyll fluorescence. Nonphotochemical quenching depends primarily on the [delta]pH that is generated by photosynthetic electron transport, and it is also correlated with the amounts of zeaxanthin and antheraxanthin that are formed from violaxanthin by the operation of the xanthophyll cycle. To perform a genetic dissection of nonphotochemical quenching, we have isolated npq mutants of Chlamydomonas by using a digital video-imaging system. In excessive light, the npq1 mutant is unable to convert violaxanthin to antheraxanthin and zeaxanthin; this reaction is catalyzed by violaxanthin de-epoxidase. The npq2 mutant appears to be defective in zeaxanthin epoxidase activity, because it accumulates zeaxanthin and completely lacks antheraxanthin and violaxanthin under all light conditions. Characterization of these mutants demonstrates that a component of nonphotochemical quenching that develops in vivo in Chlamydomonas depends on the accumulation of zeaxanthin and antheraxanthin via the xanthophyll cycle. However, observation of substantial, rapid, [delta]pH-dependent nonphotochemical quenching in the npq1 mutant demonstrates that the formation of zeaxanthin and antheraxanthin via violaxanthin de-epoxidase activity is not required for all [delta]pH-dependent nonphotochemical quenching in this alga. Furthermore, the xanthophyll cycle is not required for survival of Chlamydomonas in excessive light.

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Selected References

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  • Adams WW, Demmig-Adams B, Winter K. Relative contributions of zeaxanthin-related and zeaxanthin-unrelated types of ;high-energy-state' quenching of chlorophyll fluorescence in spinach leaves exposed to various environmental conditions. Plant Physiol. 1990 Feb;92(2):302–309. [PMC free article] [PubMed]
  • Aro EM, Virgin I, Andersson B. Photoinhibition of Photosystem II. Inactivation, protein damage and turnover. Biochim Biophys Acta. 1993 Jul 5;1143(2):113–134. [PubMed]
  • Balachandran S, Osmond CB, Daley PF. Diagnosis of the Earliest Strain-Specific Interactions between Tobacco Mosaic Virus and Chloroplasts of Tobacco Leaves in Vivo by Means of Chlorophyll Fluorescence Imaging. Plant Physiol. 1994 Mar;104(3):1059–1065. [PMC free article] [PubMed]
  • Bennoun P, Levine RP. Detecting mutants that have impaired photosynthesis by their increased level of fluorescence. Plant Physiol. 1967 Sep;42(9):1284–1287. [PMC free article] [PubMed]
  • Cogdell RJ, Frank HA. How carotenoids function in photosynthetic bacteria. Biochim Biophys Acta. 1987;895(2):63–79. [PubMed]
  • Davies JP, Yildiz F, Grossman AR. Mutants of Chlamydomonas with Aberrant Responses to Sulfur Deprivation. Plant Cell. 1994 Jan;6(1):53–63. [PMC free article] [PubMed]
  • Davies JP, Yildiz FH, Grossman A. Sac1, a putative regulator that is critical for survival of Chlamydomonas reinhardtii during sulfur deprivation. EMBO J. 1996 May 1;15(9):2150–2159. [PMC free article] [PubMed]
  • Debuchy R, Purton S, Rochaix JD. The argininosuccinate lyase gene of Chlamydomonas reinhardtii: an important tool for nuclear transformation and for correlating the genetic and molecular maps of the ARG7 locus. EMBO J. 1989 Oct;8(10):2803–2809. [PMC free article] [PubMed]
  • Gilmore AM, Hazlett TL, Govindjee Xanthophyll cycle-dependent quenching of photosystem II chlorophyll a fluorescence: formation of a quenching complex with a short fluorescence lifetime. Proc Natl Acad Sci U S A. 1995 Mar 14;92(6):2273–2277. [PMC free article] [PubMed]
  • Grossman AR, Bhaya D, Apt KE, Kehoe DM. Light-harvesting complexes in oxygenic photosynthesis: diversity, control, and evolution. Annu Rev Genet. 1995;29:231–288. [PubMed]
  • Gumpel NJ, Ralley L, Girard-Bascou J, Wollman FA, Nugent JH, Purton S. Nuclear mutants of Chlamydomonas reinhardtii defective in the biogenesis of the cytochrome b6f complex. Plant Mol Biol. 1995 Dec;29(5):921–932. [PubMed]
  • Horton P, Ruban AV, Walters RG. REGULATION OF LIGHT HARVESTING IN GREEN PLANTS. Annu Rev Plant Physiol Plant Mol Biol. 1996 Jun;47(NaN):655–684. [PubMed]
  • Hurry V, Anderson JM, Chow WS, Osmond CB. Accumulation of Zeaxanthin in Abscisic Acid-Deficient Mutants of Arabidopsis Does Not Affect Chlorophyll Fluorescence Quenching or Sensitivity to Photoinhibition in Vivo. Plant Physiol. 1997 Feb;113(2):639–648. [PMC free article] [PubMed]
  • Jansson S. The light-harvesting chlorophyll a/b-binding proteins. Biochim Biophys Acta. 1994 Feb 8;1184(1):1–19. [PubMed]
  • Lee AI, Thornber JP. Analysis of the pigment stoichiometry of pigment-protein complexes from barley (Hordeum vulgare). The xanthophyll cycle intermediates occur mainly in the light-harvesting complexes of photosystem I and photosystem II. Plant Physiol. 1995 Feb;107(2):565–574. [PMC free article] [PubMed]
  • Maxwell DP, Falk S, Huner NPA. Photosystem II Excitation Pressure and Development of Resistance to Photoinhibition (I. Light-Harvesting Complex II Abundance and Zeaxanthin Content in Chlorella vulgaris). Plant Physiol. 1995 Mar;107(3):687–694. [PMC free article] [PubMed]
  • MEHLER AH. Studies on reactions of illuminated chloroplasts. I. Mechanism of the reduction of oxygen and other Hill reagents. Arch Biochem Biophys. 1951 Aug;33(1):65–77. [PubMed]
  • Meurer J, Meierhoff K, Westhoff P. Isolation of high-chlorophyll-fluorescence mutants of Arabidopsis thaliana and their characterisation by spectroscopy, immunoblotting and northern hybridisation. Planta. 1996;198(3):385–396. [PubMed]
  • Miles CD, Daniel DJ. Chloroplast Reactions of Photosynthetic Mutants in Zea mays. Plant Physiol. 1974 Apr;53(4):589–595. [PMC free article] [PubMed]
  • Pazour GJ, Sineshchekov OA, Witman GB. Mutational analysis of the phototransduction pathway of Chlamydomonas reinhardtii. J Cell Biol. 1995 Oct;131(2):427–440. [PMC free article] [PubMed]
  • Rock CD, Zeevaart JA. The aba mutant of Arabidopsis thaliana is impaired in epoxy-carotenoid biosynthesis. Proc Natl Acad Sci U S A. 1991 Sep 1;88(17):7496–7499. [PMC free article] [PubMed]
  • Ruban AV, Young AJ, Pascal AA, Horton P. The Effects of Illumination on the Xanthophyll Composition of the Photosystem II Light-Harvesting Complexes of Spinach Thylakoid Membranes. Plant Physiol. 1994 Jan;104(1):227–234. [PMC free article] [PubMed]
  • Phillip D, Ruban AV, Horton P, Asato A, Young AJ. Quenching of chlorophyll fluorescence in the major light-harvesting complex of photosystem II: a systematic study of the effect of carotenoid structure. Proc Natl Acad Sci U S A. 1996 Feb 20;93(4):1492–1497. [PMC free article] [PubMed]
  • Ruban AV, Phillip D, Young AJ, Horton P. Carotenoid-dependent oligomerization of the major chlorophyll a/b light harvesting complex of photosystem II of plants. Biochemistry. 1997 Jun 24;36(25):7855–7859. [PubMed]
  • Tam LW, Lefebvre PA. Cloning of flagellar genes in Chlamydomonas reinhardtii by DNA insertional mutagenesis. Genetics. 1993 Oct;135(2):375–384. [PMC free article] [PubMed]
  • Tardy F, Havaux M. Photosynthesis, chlorophyll fluorescence, light-harvesting system and photoinhibition resistance of a zeaxanthin-accumulating mutant of Arabidopsis thaliana. J Photochem Photobiol B. 1996 Jun;34(1):87–94. [PubMed]

Articles from The Plant Cell are provided here courtesy of American Society of Plant Biologists


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