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J Clin Microbiol. Jun 2003; 41(6): 2348–2357.
PMCID: PMC156557

The Genus Aeromonas: Biochemical Characteristics, Atypical Reactions, and Phenotypic Identification Schemes


A total of 193 strains representing 14 different Aeromonas genomospecies were evaluated for 63 phenotypic properties to create useful tables for the reference identification of mesophilic aeromonads. Only 9 of 62 biochemical tests (14%) yielded uniform results, and the fermentation of certain carbohydrates was found to be linked to specific species. A number of unusual or aberrant properties for the genus Aeromonas were also detected in the collection of 428 strains (193 in the phenotypic study, 235 in a retrospective review). These tests included susceptibility to the vibriostatic agent, fermentation of m-inositol and d-xylose, hydrolysis of urea, and the lack of cytochrome oxidase activity. Fermentation of melibiose was linked to raffinose fermentation in all Aeromonas species except A. jandaei. Keys are provided for clinical laboratories choosing to identify aeromonads to species level based upon initial Møeller decarboxylase and dihydrolase reactions. In addition, several new tests were identified that help to separate members of the A. caviae complex (A. caviae, A. media, and A. eucreonophila).

The genus Aeromonas has undergone a number of taxonomic and nomenclature revisions over the past 15 years. Although originally placed in the family Vibrionaceae (34), which also included the genera Vibrio, Photobacterium, and Plesiomonas, subsequent phylogenetic investigations indicated that the genus Aeromonas is not closely related to vibrios but rather forms a monophyletic unit in the γ-3 subgroup of the class Proteobacteria (25, 31). These conclusions necessitated the removal of Aeromonas from the family Vibrionaceae and transfer to a new family, the Aeromonadaceae (8). Similarly, only five species of Aeromonas were recognized 15 years ago (21), three of which (A. hydrophila, A. sobria, and A. caviae) existed as phenospecies, that is, a named species containing multiple DNA groups, the members of which could not be distinguished from one another by simple biochemical characteristics. Subsequent systematic investigations have resulted in the number of valid published genomospecies rising to 14 (23), and it is anticipated that additional species will be described because rare strains have been identified that do not reside in any established Aeromonas species.

The genus Aeromonas comprises important human pathogens causing primary and secondary septicemia in immunocompromised persons, serious wound infections in healthy individuals and in patients undergoing medicinal leech therapy, and a number of less well described illnesses such as peritonitis, meningitis, and infections of the eye, joints, and bones (18). Gastroenteritis, the most common clinical manifestation, remains controversial (18). While there are a number of well-described cases of Aeromonas-associated gastroenteritis in the literature, it still remains unproven whether most fecal isolates recovered from symptomatic persons are the etiologic agent responsible for the diarrheal syndrome. One theory to explain this contradiction is that only specific subsets of Aeromonas are pathogenic for humans and that biotyping schemes need to be developed to differentiate environmental from clinical strains (23).

One of the major difficulties in the identification of Aeromonas strains to species level concerns the current number of recognized taxa (n = 14) and the lack of clear-cut phenotypic tables useful in distinguishing each of these groups from the others (17). This problem has arisen because taxonomic studies often report only selected biochemical characteristics on newly described species and then compare these results to phenotypic data from previously published studies on genetically related taxa. Although the tests used may be the same in each study, the growth conditions, medium composition, inoculation procedure, and incubation conditions may vary considerably, potentially affecting results (12, 15).

In some instances, commercial systems have been used to generate the data, and it is not always clear how closely microidentification test results parallel results obtained by conventional methodology (2). Furthermore, many of the biochemical schemes currently used in clinical laboratories to identify aeromonads predate the description of newer taxa (1, 5, 7, 22). This fact calls into question whether previously selected biochemical tests used to identify older Aeromonas species are applicable to the identification of those described more recently. Finally, the diversity in testing methodologies has hampered the development of consistent phenotypic properties and typing schemes with which to identify most Aeromonas strains, if necessary, to species level in the clinical laboratory.

In this study we present cumulative biochemical data on almost 200 Aeromonas strains representing each of the 14 recognized species and propose possible schemes for their identification in the clinical laboratory (Table (Table11).

Type and reference cultures of Aeromonas strains used in this investigationa



A total of 193 strains were investigated in this study (Table (Table1);1); approximately 75% were from clinical specimens, with the remainder (≈25%) from animals, fish, or environmental sources. The taxa included in these investigations were A. hydrophila, A bestiarum, A. salmonicida, A. caviae, A. media, A. eucrenophila, A sobria (sensu stricto), A. veronii (two biotypes), A. jandaei, A. schubertii, A. trota, A. encheleia, A. allosaccharophila, and A. popoffii. Included within these 193 strains were the type strains for 13 Aeromonas species and 37 reference strains received from national and international culture collections. The A. salmonicida type strain, ATCC 33658T (subspecies salmonicida), was not included in this study as it represents strains typically recovered from fish (salmonids) that grow at lower temperatures (20°C), produce a brownish pigment, and are nonmotile and indole negative. The A. salmonicida strains included in this study primarily originated from clinical material (feces) and from environmental samples (water). These strains were motile, grew well at 35°C, were indole positive, and did not produce melanin-like pigments; however, they did belong to hybridization group 3 (A. salmonicida) by DNA pairing studies.

Of these 193 strains, 152 (79%) had previously been identified to species level by DNA binding. The remaining 41 strains fit the classic phenotypic definition for their respective taxa. All strains were maintained as working cultures in motility agar deeps at room temperature during the course of these investigations and were periodically transferred to retain viability. In addition to this initial group of 193 strains, we retrospectively reviewed biochemical data on another 235 isolates in our collection.

Biochemical tests.

Aeromonas strains (n = 193) were tested for 63 phenotypic traits. These tests were performed in a conventional format as previously described, and appropriate positive and negative controls were included for each test and with each lot of prepared medium (1, 13, 19). Liquid medium or agar slants were inoculated from overnight tryptone broth cultures grown at 35°C or, in the case of A. popoffii and A. sobria CIP 7433 (sensu stricto), 25°C. Plates (e.g., to assay elastase) were inoculated from overnight growth on heart infusion agar slants. Biochemical or enzymatic tests performed by plate assays included DNase and elastase activities, elaboration of a β-hemolysin, polypectate (pectinase) degradation, and expression of a stapholysin (1, 16).

Several new tests were added, including clearing of tyrosine-containing (0.5%) plates (tyrosinase), detection of alkylsulfatase activity (plate), and Jordan's tartrate (13, 20). Carbohydrate fermentation reactions were performed in extract broth (Acumedia Manufacturers, Inc., Baltimore, Md.) containing 1% (wt/vol) of the desired sugar and 1% (vol/vol) Andrade's indicator. All reagents (sugars and substrates) were obtained from Sigma (St. Louis, Mo.). Gas production from d-glucose fermentation was determined in carbohydrate fermentation broths containing Durham tubes. All tests were incubated at 35°C (polypectate and pigment, 25°C) with the exception of A. popoffii and A. sobria CIP 7433 (sensu stricto), which were incubated at 25°C because preliminary experiments had indicated that much better growth occurred at lower rather than higher temperatures.

Tests were read daily for 7 days with the following exceptions: tests for o-nitrophenyl-β-d-galactopyranoside (ONPG) and susceptibility to O/129 (2,4-diamino-6,7-diisopropylpteridine, 150 μg), ampicillin (10 μg), and cephalothin (30 μg) were read at 1 day; tests for KCN, malonate, gluconate oxidation, pyrazinamidase, and Jordan's tartrate were read at 2 days; tests for dl-lactate and urocanic acid utilization and Voges-Proskauer were read at 3 days; and tests for urea hydrolysis, citrate utilization, ornithine and lysine decarboxylase, and arginine dihydrolase activity were read at 4 days. A subset of these strains (n = 27) were additionally evaluated for the ability to ferment rare or unusual sugars. Carbohydrates tested included β-gentobiose, glucamine, glucose 1-phosphate, glucose 6-phosphate, inulin, lactulose, d-lyxose, maltotriose, palatinose, sedoheptulose, stachyose, d-tagatose, d-turanose, and xylitol. In addition, to determine the relative extent of phenotypic variation in the genus, the biochemical test results of 235 additional Aeromonas strains identified by the Microbial Diseases Laboratory were reviewed.


Biochemical properties.

Most Aeromonas strains produced tan to buff-colored colonies on Trypticase soy agar (Becton-Dickinson, Cockeysville, Md.) when incubated at 25°C for 2 to 5 days. A. encheleia was extremely slow in pigment production, with all four strains yielding tan colonies only after 7 days of incubation. Rare strains, such as A. bestiarum ATCC 14715, A. media ATCC 33907 and ATCC 35950, and a strain of A. eucrenophila, produced brown to dark brown colonies on Trypticase soy agar. This pigmentation was similar to the melanin-like pigment produced by many fish isolates of A. salmonicida (10).

Of the 62 biochemical characteristics evaluated for all 193 strains of Aeromonas, only 9 tests (15%) yielded uniform results. These reactions were the presence of cytochrome oxidase and nitrate reductase, fermentation of d-glucose and trehalose, failure to utilize mucate, and the inability to produce acid from d-arabitol, dulcitol, erythritol, and xylose. The remaining 53 tests that yielded variable results are listed in Table Table2.2. The positive reactions listed in Table Table22 were for 48 h (2 days). This endpoint was chosen because clinical laboratories rarely read biochemical test results on rapid growers for more than 48 h. Although all 193 Aeromonas strains were oxidase positive, several other tests useful in the differentiation of aeromonads from vibrios and plesiomonads gave variable results (Table (Table22).

Biochemical properties of Aeromonas species

Two strains of A. eucrenophila and one strain of A. veronii biogroup veronii produced small zones of growth inhibition in the presence of the vibriostatic agent O/129. One A. caviae strain required 72 h to grow in nutrient broth containing 0% and 3% NaCl. Both A. sobria strains (sensu stricto, fish isolates), one A. bestiarum, and one A. eucrenophila strain failed to grow in nutrient broth supplemented with 3% salt. A strain of A. allosaccharophila was found to ferment m-inositol, a characteristic typically associated with Plesiomonas shigelloides. Three enzymatic activities, production of elastase, polypectate (pectinase) degradation, and hydrolysis of Staphylococcus aureus cell wall components (stapholysin), were associated only with the A. hydrophila complex (A. hydrophila sensu stricto, A. bestiarum, and A. salmonicida). The fermentation (or lack thereof) of some methyl pentose, disaccharides, and alcohol carbohydrates was also linked to specific genomospecies.

Most strains that fermented l-rhamnose belonged to A. bestiarum, A. encheleia, or A. allosaccharophila. Almost half of all A. jandaei strains fermented the disaccharide melibiose. Fermentation of d-sorbitol, previously linked to A. salmonicida (19), was almost exclusively associated with this species, as 85% of strains tested fermented this alcoholic sugar; only one other strain with similar abilities (A. caviae) was identified in this survey. The inability to ferment d-mannitol was primarily restricted to A. schubertii (14) and some strains of A. trota (6).

Fermentation of unusual carbohydrates.

Because many Aeromonas species are difficult to identify with a limited number of biochemical characteristics, we explored whether the fermentation of unusual carbohydrates might be useful as an aid to species identification. Thirteen type strains and 37 reference strains of Aeromonas (Table (Table1)1) representing each of the 14 nomenspecies were used to screen for potentially useful characters. Eight carbohydrates yielded uniformly negative test results: β-gentobiose, glucamine, inulin, d-lyxose, sedoheptulose, stachyose, d-tagatose, and xylitol. All 27 strains fermented maltotriose, although most isolates (>80%) required 96 h to produce acid from this carbohydrate. Palatinose was fermented by five of six strains of the A. hydrophila complex, by both A. popoffii strains tested, and by one A. veronii biogroup veronii isolate. Subsequently all A. popoffii strains were tested and found to be palatinose positive. Turanose fermentation was detected in only 5 of the 27 strains tested (19%) and then only after prolonged incubation (4 to 7 days). Three other sugars (lactulose, glucose 1-phosphate, and glucose 6-phosphate) yielded potentially discriminatory results (see A. caviae complex).

Atypical phenotypic properties.

In addition to the data presented in Table Table2,2, we retrospectively reviewed laboratory data on an additional 235 Aeromonas strains. These strains were identified to genomospecies level as A. hydrophila, A. caviae, A. veronii biogroup sobria, or Aeromonas sp. (could not be placed in a defined taxon based upon biochemical characteristics). Of the 12 characteristics listed in Table Table3,3, five of these phenotypes were not detected in the original survey of 193 strains (Table (Table2).2). These tests include fermentation of d-arabitol and d-xylose, mucate utilization, and failure to produce cytochrome oxidase and acid from trehalose. The most common atypical biochemical characteristics observed included fermentation of l-rhamnose, d-sorbitol, and melibiose and urea hydrolysis. An oxidase-negative strain was recovered from the feces of a 16-day-old male infant with gastroenteritis in 1992. Because of the negative oxidase reaction, it was originally thought to be a possible Chromobacterium violaceum isolate. Subsequent biochemical testing in our laboratory identified this strain as an oxidase-negative A. caviae complex member (confirmed by the Centers for Disease Control).

Atypical phenotypic properties of the genus Aeromonas

Coexpression of raffinose and melibiose fermentation.

Twenty strains were identified that fermented either melibiose (Mel+) or raffinose (Raf+) or both among the 428 strains analyzed (Table (Table2,2, Table Table3).3). With the exception of A. jandaei (n = 7), for which all strains were Mel+ Raf, the remaining 13 strains (63%) were uniformly Mel+ Raf+ (Table (Table4).4). These Mel+ Raf+ strains were isolated over a 17-year period and were recovered from diverse clinical and environmental specimens. Expression of the Mel+ Raf+ phenotype was rapid (24 to 48 h) and was observed in four different genomospecies plus a number of isolates (n = 5) that could not be assigned to a taxon.

Propeties of raffinose- and melibiose-positive Aeromonas strains

Identification schemes for aeromonads.

Because of the increasing number of recognized Aeromonas species and the number of strains with unusual or atypical biochemical reactions, it is becoming increasingly difficult to identify older and newer members of this genus. One approach to identifying aeromonads to species level would be to rely on the results of Møeller decarboxylase and dihydrolase reactions to narrow the list of potential groups to a minimum (Fig. (Fig.1).1). For instance, at present ornithine decarboxylase-positive strains (group 1) could only be either A. veronii biogroup veronii (most common) or A. allosaccharophila (one strain to date), while lysine decarboxylase-negative, ornithine decarboxylase-negative, and arginine dihydrolase-negative strains (group 3) would be restricted to the A. caviae complex (A. caviae, A. media, and A. eucrenophila) or to A. encheleia. However, by far the majority of clinical isolates would fall into either group 2 or 4, requiring additional tests to identify isolates to either the complex or genomospecies level.

FIG. 1.
Use of Møeller decarboxylase and dihydrolase reactions as a screening tool for recognition of potential species giving the indicated reaction. *, only rare strains of these species display this pattern. Abbreviations: LDC, lysine decarboxylase; ...

Strains could be placed into one of these three complexes based upon a limited number of tests, most of which are either included in commercial identification systems or routinely used in laboratories to identify other groups of bacteria (Table (Table5).5). A. popoffii (n = 7), A. encheleia (n = 4), A. allosaccharophila (n = 3), and A. sobria (n = 2) were not included because of the limited number of available strains and unusual growth requirements (25°C) for some species (A. popoffii). For instance, strains that were both esculin negative (Esc) and l-arabinose negative (Ara) would be restricted to the A. sobria (phenospecies) complex, as only one strain outside of this group (an A. eucrenophila isolate) was Esc Ara. Similarly, negative Voges-Proskauer and/or gas from glucose reactions would help separate the A. caviae complex from A. trota strains, which are lysine decarboxylase positive, and A. schubertii strains, which are Ara. However, three strains of A. bestiarum which were lysine decarboxylase negative, Voges-Proskauer negative, and gas-from-glucose negative would be misidentified to complex level with this system.

Biochemical identification of Aeromonas to complex level

A. hydrophila complex.

As previously mentioned, any Aeromonas strain producing the enzyme elastase, pectinase, or stapholysin belongs to the A. hydrophila complex. No strain of A. encheleia or A. popoffii was found to produce these activities. Tests useful in differentiating members of the A. hydrophila complex are listed in Table Table6.6. Of the tests listed, the single most useful test was fermentation of d-sorbitol, which detected 85% of A. salmonicida strains. Other tests useful in separating members of this complex include utilization of dl-lactate and urocanic acid. Fermentation of l-rhamnose was a marker associated with a majority of A. bestiarum strains, but almost one-quarter of all A. hydrophila strains also produced acid from this sugar. Simmon's citrate was a useful test at 48 h, as was lactose fermentation. However, upon prolonged incubation (4 and 7 days, respectively), many A. hydrophila strains became positive, reducing the usefulness of these assays.

Tests useful in the separation of members of the A. hydrophila complex

A. caviae complex.

Tests useful in differentiating members of the A. caviae complex (A. caviae, A. media, and A. eucrenophila) are listed in Table Table7.7. In addition to previously described tests to separate members of this complex (1), several new tests useful in identifying members to genomospecies were found, including dl-lactate and urocanic acid utilization and fermentation of glucose 1-phosphate, glucose 6-phosphate, and lactulose. The latter three tests were identified in a limited survey of all Aeromonas species for the ability to ferment rare or unusual carbohydrates. When these tests were recognized, all 45 isolates of this complex were tested for acid production from glucose 1-phosphate, glucose 6-phosphate, and lactulose. A number of other tests were also useful in separating members of the A. caviae complex but were not as differential as those listed in Table Table7.7. These tests include fermentation of sucrose, glycerol, and phenylpyruvic acid (1). Some tests formerly found to be useful (1), including β-hemolysis and H2S production from cysteine, were less discriminatory in the resolution of species.

Tests useful in the separation of members of the A. caviae complex

Several phenotypic changes within A. caviae strains (sensu stricto) were also noted in this study. The Microbial Diseases Laboratory began seeing urea-hydrolyzing (Ure+) A. caviae strains as early as 1984, and this phenotype peaked between 1988 and 1991. Serotyping of random Ure+ A. caviae strains (courtesy of T. Shimada, National Institutes of Health, Tokyo, Japan) indicated that these isolates fell into several serogroups, including O:61, O:62, and OUK (unknown serogroup). A second noted difference was the frequency of β-hemolytic A. caviae strains. Early investigations found β-hemolysis to be a useful test in separating the A. caviae complex (usually negative) from the hemolytic phenospecies A. hydrophila and A. sobria (2, 16, 26, 30). However, in this study, over half (52%) of all A. caviae strains tested were beta-hemolytic on sheep blood agar within 48 h (Table (Table2).2). Furthermore, a retrospective review of A. caviae strains submitted to our laboratory for identification since 1996 indicated that 89% of these A. caviae isolates are beta-hemolytic.

A. sobria complex.

In 1976, Popoff and Véron (28) identified what later turned out to be the A. sobria complex or phenospecies. The A. sobria complex was defined on the basis of a number of phenotypic traits, which included failure to hydrolyze esculin, failure to ferment (or utilize) l-arabinose and salicin, and failure to grow in KCN broth. With some minor exceptions (e.g., variable growth in KCN broth for some species), this complex is composed of the following nomenspecies: A veronii biogroup sobria (DNA hybridization group 8), A. jandaei, A. schubertii, and A. trota. The major phenotypic features useful in the separation of species within the A. sobria complex are listed in Table Table8.8. Although A. veronii biogroup sobria and A. jandaei can only be distinguished from one another in Table Table88 based upon sucrose fermentation, there are several other tests useful in separating these taxa. Two-thirds of A. jandaei strains are resistant to cephalothin, while all A. veronii biogroup sobria strains were susceptible or partially susceptible to this first-generation cephalosporin. Half of the A. veronii biogroup sobria strains were pyrazinamidase positive, and a third degraded l-tyrosine crystals; neither activity was associated with A. jandaei isolates. Also, most A. jandaei were citrate positive, while only half of A. veronii biogroup sobria strains utilized citrate. As previously mentioned, almost half (47%) of A. jandaei fermented melibiose, while only 1 of 25 A. veronii biovar sobria strains fermented this carbohydrate.

Tests useful in the separation of members of the A. sobria complex

Both A. schubertii and A. trota were easily recognizable by exhibiting different reactions from both A. veronii biovar sobria and A. jandaei in the indole, Voges-Proskauer, and lipase tests and susceptibility to ampicillin. Several sugar reactions were additionally useful, although fermentation of glycerol by A. schubertii strains lost diagnostic significance upon prolonged incubation (>48 h) because many strains showed a delayed fermentation of this carbohydrate.


The results of the present investigation further document the extensive phenotypic diversity within the genus Aeromonas and of the 14 currently recognized Aeromonas species (Table (Table2).2). Of the more than 60 tests evaluated in the present investigation, only four characteristics exhibited uniform reactions for the more than 400 Aeromonas strains evaluated, 193 in the prospective study and 235 in the retrospective review. These four tests were fermentation of d-glucose, production of nitrate reductase, and failure to produce acid from either dulcitol or erythritol. The reasons for the increasing phenetic diversity observed in the genus are numerous. As new taxa are reported, unusual phenotypic properties for the genus are often described, such as in the case of d-mannitol-negative A. schubertii (14) and ampicillin-susceptible A. trota (6). Furthermore, as surveys analyze strains from environmental sources, an increase in phenotypic diversity from the normalized data (profiles) established for clinical strains should be expected (4). Examples of such variation include the isolation of d-xylose-positive Aeromonas strains from the Chesapeake Bay (27) and O/129-sensitive isolates recovered from Japanese tadpoles (33).

For less well-characterized species, the true extent of phenotypic variation remains unknown, although, as more strains of these uncommon genomospecies are identified, greater phenetic diversity is likely to be found. Such diversity can be seen in the recent case report describing the isolation of an A. media strain that is lysine decarboxylase positive from the sputum of a patient with chronic bronchitis (11). The bottom line to this phenotypic diversity is that it will become increasingly difficult to identify Aeromonas isolates to species level without extensive arrays of biochemical tests. Fortunately, approximately 85% of clinical isolates fall into one of three recognizable genomospecies, that is, A. hydrophila (sensu stricto, HG1), A. caviae (sensu stricto, HG4), and A. veronii biotype sobria (sensu stricto, HG8, often incorrectly referred to as A. sobria).

Although increasing phenotypic diversity within the genus is now being recorded, Aeromonas isolates can in most cases be identified to phenospecies or genomospecies with fairly straightforward biochemical schemes and selected biochemical characteristics (Tables (Tables55 to to8).8). Some tests, previously used in a number of identification schemes, now seem to be less useful than previously thought due to the expanding number of species and the need to generate an identification within a reasonable period of time (48 h). These tests include growth in KCN broth, fermentation of salicin, and production of a β-hemolysin. Growth in KCN, a test not commonly used by most clinical laboratories, appears less helpful now, since 60% of A. veronii biogroup sobria and A. jandaei strains grew in this broth. Likewise, fermentation of salicin has lost some of its discriminatory value, as 25% to 70% of members of the A. hydrophila complex fail to produce acid from this aglycone. Finally, the widespread emergence of beta-hemolytic A. caviae strains, one of the three most common species identified in the clinical laboratory, renders this trait of limited value in distinguishing A. caviae from A. hydrophila (sensu stricto) and A. veronii biovar sobria.

A 1996 Canadian study of 35 A. caviae strains of clinical origin found 6 (17%) to be beta-hemolytic on sheep blood agar plates (35). The hemolysin detected in that investigation appears to be unique to A. caviae. It may be that the increased incidence of hemolytic A. caviae strains is due to dissemination of clones bearing this hemolytic determinant or to horizontal transfer of hemolysin genes from other hemolytic Aeromonas species (e.g., A. hydrophila and A. veronii biotype sobria) to A. caviae. Whatever the reason, the incidence of beta-hemolytic A. caviae strains is on the rise and warrants attention, as it limits the use of this marker as an aid to species identification.

An interesting finding was the coexpression of rapid melibiose and raffinose fermentation by some strains of several Aeromonas species, excluding A. jandaei. This was an unexpected observation and suggests that these markers may be closely linked on the bacterial chromosome. Another possibility is that Mel+ Raf+ strains may be harbored on extrachromosomal elements that encode these metabolic activities. This hypothesis seems unlikely since most aeromonads do not routinely carry plasmids (only ≈25%), although it cannot be ruled out at present and will require further investigation. Similar to this observation was the initial presence of Ure+ A. caviae strains in California in the late 1980s and early 1990s. These strains seem to have mostly disappeared by the mid-1990s and were most often associated with diarrheal disease. Because the serotype could not be determined for several strains, it is possible that one or more clones carrying the Ure+ marker emerged during this period and subsequently declined in numbers. The reasons for this possible decline are unknown. However, in Yersinia enterocolitica, urease activity contributes to acid tolerance and may promote bacterial survival prior to infection (24). Thus, urease activity in select Aeromonas strains might provide a similar advantage.

The most important decision facing clinical laboratories is how far to proceed with the identification of Aeromonas species isolated from clinical material. For most moderate- to larger-sized hospitals or medical centers, it seems reasonable that isolates should be identified at least to phenospecies (Table (Table5).5). However, under a number of additional circumstances, medical facilities may want to proceed with definitive identifications. Some cases of gastroenteritis may fall into this category. Patients with hematologic malignancies may be more prone to gastrointestinal tract colonization with aeromonads than persons with other underlying conditions (32). Since the gastrointestinal tract is often the anatomic site from which bacteria disseminate to produce septicemia, and the pathogenic (invasive) potential of Aeromonas species varies, identifying isolates to species may be warranted in order to monitor such persons.

A second situation where species identification of strains may be justified is in reputed cases of chronic disease, such as gastroenteritis (29) or hepatobiliary disease (unpublished observations). In these instances, identifying Aeromonas isolates to species may resolve issues concerning whether a patient has chronic disease or has been reinfected by a different strain. Although no definitive outbreak involving Aeromonas has ever been described, there have been clusters of cases reported at long-term care (3) and day care (9) facilities where identification of strains to species was appropriate. Finally, isolates recovered from systemic infections such as blood should be good candidates for species identification, since it will help define the role of each genomospecies in serious clinical disease, thereby impacting prognosis and treatment. Potential useful tests for the identification of aeromonads by both clinical and reference microbiology laboratories under these circumstances are listed in Table Table99.

Suggested and recommended tests useful in Aeromonas identification schemes


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