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J Clin Invest. Sep 1, 2006; 116(9): 2423–2433.
Published online Sep 1, 2006. doi:  10.1172/JCI28941
PMCID: PMC1555646

Human CD4+ CD25hi Foxp3+ regulatory T cells are derived by rapid turnover of memory populations in vivo


While memory T cells are maintained by continuous turnover, it is not clear how human regulatory CD4+CD45RO+CD25hi Foxp3+ T lymphocyte populations persist throughout life. We therefore used deuterium labeling of cycling cells in vivo to determine whether these cells could be replenished by proliferation. We found that CD4+CD45RO+Foxp3+CD25hi T lymphocytes were highly proliferative, with a doubling time of 8 days, compared with memory CD4+CD45RO+Foxp3CD25 (24 days) or naive CD4+CD45RA+Foxp3CD25 populations (199 days). However, the regulatory population was susceptible to apoptosis and had critically short telomeres and low telomerase activity. It was therefore unlikely to be self regenerating. These data are consistent with continuous production from another population source. We found extremely close TCR clonal homology between regulatory and memory CD4+ T cells. Furthermore, antigen-related expansions within certain TCR Vβ families were associated with parallel numerical increases of CD4+CD45RO+CD25hiFoxp3+ Tregs with the same Vβ usage. It is therefore unlikely that all human CD4+CD25+Foxp3+ Tregs are generated as a separate functional lineage in the thymus. Instead, our data suggest that a proportion of this regulatory population is generated from rapidly dividing, highly differentiated memory CD4+ T cells; this has considerable implications for the therapeutic manipulation of these cells in vivo.


Both memory and regulatory populations of T cells must be maintained in tandem in order to generate controlled immunity for the lifetime of the organism. Since the thymus involutes early in life, memory T cells have to largely be maintained by lifelong turnover of preexisting populations of specific T cells in adults (1, 2). The corollary of this is that thymic involution during aging will also severely restrict the production of Tregs by this organ. The source of these cells in adult humans and the relative contributions of long-term survival and ongoing turnover to the maintenance of CD4+CD25hiFoxp3+ Treg populations remain unknown.

The naturally occurring CD4+CD25hi Treg subset that expresses the lineage marker Foxp3 represents an important population of suppressive T cells that can prevent reactivity to both self and nonself antigens (35). These cells also downregulate immune responses as pathogen is cleared (35). Early studies demonstrated that in mice, CD4+CD25hi Tregs are generated as a distinct population in the thymus (6). Indeed, in mice, there is substantial overlap of TCR repertoires between thymic and peripheral CD4+Foxp3+ Tregs, suggesting that the thymic regulatory pool makes a significant contribution to the peripheral regulatory cells (7). However, murine CD4+CD25hi Tregs, which are phenotypically and functionally identical to the thymus-derived population can also be generated after antigen-induced proliferation of CD4+ T cells in peripheral tissues (810).

Programmed cell death or apoptosis represents an important control mechanism during the activation and expansion of memory T cells during each reencounter with antigen (1113). Furthermore, the residual replicative capacity of these cells may be limited by telomere erosion (14, 15), which eventually leads to cell cycle arrest and is a mechanism that may limit the proliferative capacity of memory T cells during repeated stimulation with antigen (14, 16). Although CD4+CD25hi Tregs have been shown to divide rapidly in murine systems (1719), humans have a much longer life span but much shorter telomeres than mice (20). This suggests that the lifelong persistence of human CD4+CD25hi Tregs by continuous proliferation may be limited. In addition, if human CD4+CD25+ Tregs are produced throughout life and continue to be produced after thymic involution, important questions arise about their clonal origin, particularly whether it is shared with or independent of memory T cells.

In order to investigate the turnover rates of CD4+CD25+ Tregs in humans, we used novel stable isotope technology to label proliferating cells with deuterium in vivo. To address the origin of these cells, we also investigated the relationship between the clonality of memory and Tregs by heteroduplex analysis. Collectively, our data show that human CD4+CD25hi Tregs do not arise solely from thymic generation but can also be induced by rapid turnover from the memory T cell pool. However, once generated, they are susceptible to apoptosis and have limited replicative potential. This suggests that antigenic persistence will induce continuous generation of antigen-specific Tregs from the responsive population but that, in the absence of antigen, such populations are likely to disappear. This may contribute to maintenance of tolerance toward autologous and dietary antigens in vivo yet allow for subsequent responsiveness to antigens that are encountered intermittently.


CD4+ CD25+ Foxp3+ Tregs are maintained during aging in humans.

If the thymus were the major source of CD4+CD25+Foxp3+ Tregs in adults, the number of such cells would be expected to decline with aging. To address this issue we first investigated whether the number of CD4+CD25hi T cells was altered in older (>70 years) compared with younger (<35 years) subjects. As previous studies have shown that only the CD4+ T cell population with the highest CD25 expression contains Treg activity (21), we used a stringent gating approach as detailed in Methods. We found that CD4+CD25hi cells actually constituted a greater proportion of the CD4+ T cell pool in the older subjects (mean 3.8%; n = 15) than in the younger (mean 2.7%; n = 15) (Figure (Figure1A;1A; P = 0.0119).

Figure 1
CD4+ CD25+ T cells remain functional with age.

In addition, we investigated the phenotypic and functional capacity of such cells in younger and older subjects. The CD4+CD25hi T cell populations in both groups were exclusively CD45RA and expressed lower levels of CD45RB (Supplemental Figure 1; supplemental material available online with this article; doi:10.1172/JCI28941DS1) but higher levels of CTLA-4 (data not shown) than CD4+CD25 T cells and were therefore identical qualitatively. Furthermore, CD4+CD25hi cells in both younger and older subjects showed high levels of Foxp3 expression when compared with the CD4+CD25 or CD4+CD25int populations in the same individuals (Figure (Figure1B).1B). Finally, we compared the functional capacity of CD4+CD25hi T cells from younger and older individuals by stimulating with anti-CD3 and the recall antigens tuberculin purified-protein derivative and tetanus toxoid (TT). When CD4+CD25hi T cells were added in coculture, cells from younger and older subjects showed equivalent suppressive activity on CD4+CD25 T cells (Figure (Figure1C).1C). These observations indicated that both the number and functional integrity of naturally occurring CD4+CD25hiFoxp3+ Tregs are maintained during aging.

Treg turnover in vivo.

Since the number of CD4+CD25hi Tregs was maintained during aging despite thymic involution, we investigated whether these populations were generated by ongoing proliferation, as opposed to long-term survival, in vivo. Methods have been developed recently that enable the turnover of T lymphocytes to be determined by labeling dividing cells with nonradioactive isotopes in vivo (2224). In the presence of deuterium-labeled glucose, all metabolic derivatives of glucose became proportionately labeled; this included deoxynucleosides, whose de novo synthesis pathway incorporates a pentose ring from cellular pentose pools, most of which are derived from glucose (24). Cell populations that divide therefore incorporate deuterium into their DNA in proportion to, first, the number of cell divisions within that population, and second, the level and duration of precursor (glucose) labeling. Nondividing populations remain unlabeled. We used this approach to measure proliferation and disappearance rates of CD4+CD45RO+CD25hi and CD4+CD45RO+CD25 cells in 4 younger (mean age 25 years) and 4 older subjects (mean age 81 years). Each individual received deuterium-labeled glucose for 10 hours, after which blood samples were taken on days 3, 4, 10, and 21; CD4+CD45RO+CD25hi, CD4+CD45RO+CD25, and CD4+CD45RA+ subsets were isolated by cell sorting as described in Methods (Supplemental Figure 2). Kinetics of labeling in vivo were calculated from the enrichment of deuterium in DNA of sorted T cell subsets, with higher enrichment indicating a greater portion of dividing cells (Figure (Figure2A).2A). The magnitude of peak labeling, which reflects the rate of proliferation in vivo, was significantly higher in CD4+CD45RO+CD25hi regulatory cells (7.44% of cells labeled) than in CD4+CD45RO+CD25 memory cells (2.41%) in all 8 individuals tested (P < 0.0005; Table Table1).1). The enrichment of deuterium in CD4+CD45RA+ cells was very low (0.82%; Figure Figure2,2, B and C, and Table Table1),1), consistent with slow turnover of this population. Similar differences between proliferation rates of different subsets were observed when younger and older groups were analyzed separately (Figure (Figure2,2, B and C, respectively).

Figure 2
Analysis of in vivo kinetics of human CD4+ CD25hi T cells.
Table 1
In vivo proliferative kinetics of CD4+ T cells defined according to levels of CD25 expression

When kinetics of proliferation and death were modeled to yield proliferation and disappearance rate constants (p and d*) as previously described (2, 17), significant proliferation was observed in the CD4+CD45RO+CD25hi population (mean 8.31%/day, equivalent to a doubling time [T2] of 8 days), more than in the CD4+CD45RO+CD25 populations (2.43%/day, T2 of 24 days; P = 0.0002; Table Table1).1). The T2 for the CD4+CD45RA+CD25 population was 199 days. When younger and older groups were analyzed separately, the ranges of values for proliferation of CD4+CD45RO+CD25hi cells were very similar (4.54%–9.27% and 6.16%–10.7%/day respectively; P = 0.3). Indeed, there were no significant differences between younger and older subjects in the proliferation rates of any of the cell types analyzed (Table (Table1).1). Significant disappearance rates for labeled cells (d*) were found in the CD4+CD25hi population, 6.58%/day, equivalent to a mean half-life of about 11 days, similar to the measured proliferation rate.

Our results suggest that in both younger and older individuals, human Tregs comprise a dynamic population that is continually replaced by proliferation either of CD4+CD25hi cells themselves, as observed in mice (17, 18), or of CD4+CD25 T cells that proliferate, then rapidly enter the CD25hi pool (within the 3-day interval between labeling and first blood sampling). Once within the CD4+CD25hi pool, these cells are lost rapidly. This explains the relatively constant numbers of CD4+CD25hi T cells throughout life despite such high rates of proliferation.

Constraints on the persistence of CD4+ CD25hi Tregs in vivo.

Thus, labeling data are most consistent with the continuous generation model for the origin of CD4+CD25hi Tregs. However, to further investigate the alternative hypothesis that such cells are long lived in vivo, we investigated markers of apoptosis in vivo. Our previous studies have shown that CD4+CD25+ Tregs express low levels of the antiapoptotic molecule Bcl-2 and are very susceptible to apoptosis in vitro (25). However, in these early experiments, we gated on the total CD4+CD25+ population, which included both nonregulatory CD25int and regulatory CD25hi populations. We have now extended these observations by investigating Bcl-2 expression in CD4+CD45RO+CD25hi, CD4+CD45RO+CD25int, and CD4+CD45RO+CD25 populations, using a more stringent gating strategy (as described in Figure Figure1B).1B). In both younger and older donors, Bcl-2 expression was significantly lower in the CD25hi subsets. The very strong correlation between Bcl-2 expression and death in T cells (2628) suggests that these cells are unlikely to persist because of resistance to apoptosis in vivo (Figure (Figure3A). 3A).

Figure 3
Constraints on CD4+ CD25hi T cell maintenance.

Telomere length provides an indication of both replicative history and replicative potential within a population. Each cell division leads to the loss of 50–100 bp of telomeric DNA due to the inability of DNA polymerase to replicate fully the ends of chromosomes (29). In the absence of the enzyme telomerase, this eventually leads to cell cycle arrest due to telomere erosion and is a mechanism that may limit the proliferative capacity of T cells (14, 16). Using a recently developed 3-color Flow-FISH technique, we compared the relative telomere length in CD4+CD25hi T cells in younger and older subjects and compared them with telomere lengths of total CD4+ and CD4+CD45RO+ populations. CD4+CD25hi T cells had significantly shorter telomeres than the total CD4+ T cell population in the same subjects (P = 0.002 in older and P < 0.0001 in younger; Figure Figure3B).3B). In the younger donors, CD4+CD25hi T cells also had significantly shorter telomeres than the total CD4+CD45RO+ primed/memory CD4 T cell subset (P = 0.02). Using standard curves to equate relative telomere length determined by Southern blot to relative telomere length as determined by flow-FISH (30, 31), we showed that the mean telomere length in CD4+CD25hi cells was 5.7 kb ± 0.8 kb (mean ± SD) and 4 kb ± 0.8 kb in younger and older individuals, respectively (Supplemental Figure 3). Although there appears to be a biphasic distribution of telomere length within the CD4+ population of younger subjects, this was not observed when a larger cohort of younger subjects was analyzed (32).

To determine whether CD4+CD25hi T cells were able to maintain their telomeres without further erosion upon activation, we studied their ability to upregulate telomerase upon stimulation in vitro. CD4+CD45RO+CD25hi and CD4+CD45RO+CD25 T cells were stimulated in vitro with anti-CD3– and anti-CD28–coated beads in the presence of high doses of IL-2 (200 U/ml) for 4 days. Under these conditions, CD4+CD25hi T cells were capable of limited expansion in vitro at a considerably lower level than the CD25 population (data not shown). To ensure a fair comparison, the telomerase activity analysis always included an equivalent number of proliferating CD25hi and CD25 T cells. CD4+CD25hi cells failed to upregulate telomerase compared with CD25 T cells in both younger and older subjects (Figure (Figure3C). 3C).

Previous studies have shown that in the absence of telomerase activity, telomeres shorten by 50–100 bp with every cell division and replicative senescence occurs when telomeres shorten to below 4 kb (14, 16). Combining the telomere and turnover data, since CD4+CD25hi T cells from younger subjects have a mean telomere length of 5.7 kb and a mean doubling time of 9 days (Table (Table1),1), these cells are predicted to reach senescence within 2 years. With telomeres already approaching critical length (4 kb) and a mean doubling time of 8 days, CD4+CD25hi T cells in the older subjects are already approaching senescence. The high rate of proliferation of CD4+CD25hi T cells yet only modest increase in the number of these cells during aging suggests that rapid proliferation is balanced by equally rapid cell loss, and this is evident in the calculated rate of death in the cell turnover studies (Table (Table1). 1).

The deuterium-labeling studies are unable to distinguish between loss of cells from the circulation due to death and that due to migration into tissues or phenotypic conversion. A major role for migration seems unlikely because of the early appearance in blood of recently divided CD4+CD45R0+CD25hi T cells (within 3 days; Figure Figure2)2) and the absence of late reappearance of labeled cells in the blood. Phenotypic conversion also appears unlikely since no contemporaneous increase of labeling in other CD4+ subsets was observed. Furthermore, the suggestion that the rapid loss of labeled cells was primarily due to cell death is more consistent with the observed low Bcl-2 expression (Figure (Figure3A)3A) and high susceptibility of CD4+CD45R0+CD25hi T cells to apoptosis (25).The similar disappearance rate of CD4+CD45R0+CD25 T cells (d* of 8.04%/day) relative to the CD25hi regulatory population (d* of 6.58%/day) is consistent with a similar heightened susceptibility to apoptosis of the subpopulation of actively dividing memory cells (12). Since the rapid proliferation of CD4+CD25hi T cells is balanced by rapid loss, yet CD4+CD25hi T cells are maintained at constant levels throughout life (Figure (Figure1A),1A), we conclude that these cells represent a transient population that is generated continuously in vivo.

Regulatory CD4+ CD25hi and memory CD4+ CD25 T cells are derived from the same clonal precursors.

The observation that CD4+CD25hi Tregs are unlikely to be maintained throughout life by their own continuous proliferation raised questions about the origin of the precursor population for these cells. Since memory and Tregs have to be maintained in parallel throughout life, we investigated whether these cells were derived from the same or different clonal precursor populations. When we compared the TCR repertoires of CD4+CD45RO+CD25hi and CD4+CD45RO+CD25 populations, there was striking similarity in the breadth of Vβ usage between the 2 subsets (Supplemental Figure 4).

We extended these studies by investigating the clonal relationship between CD4+CD45RO+CD25hi and CD4+CD45RO+CD25 T cell populations within each of 26 different Vβ families in the same individuals by heteroduplex analysis (Figure (Figure44 and Table Table2).2). Previous studies have shown that sharing of a clonal band by 2 different cell populations within a Vβ family indicates that the cells that give rise to the band are expanded in vivo and are derived from a precursor with the same antigenic specificity (31, 33). In 7 individuals tested, a mean of 80% of clonal bands present was shared between expanded memory and CD4+ Tregs (Table (Table2),2), showing close clonal homology, which indicated that they were most likely derived from the same precursor populations. Furthermore, when we compared the clonal distribution of CD4+CD45ROCD25hi T cells from 1 individual that were isolated at 2 different time points 7 months apart, we found that, although 33% of the clones were new and 12.5% of the clones had been lost, 60% of the clones persisted (Figure (Figure4C).4C). Thus, the picture that emerges is one of dynamic clonal evolution within the subset over time with lineages of CD4+ Tregs developing that are closely related to those within the CD4+CD45RO+CD25 memory T cell pool.

Figure 4
Regulatory CD4+ CD25hi T cells are closely related to effector CD4+ T cells.
Table 2
Clonal homology between CD4+CD25hi Tregs and CD4+CD25 memory T cells

These results suggest that antigen-driven expansion of CD4+ T cells would induce the production of CD4+CD45RO+Foxp3+CD25hi Tregs. To confirm this, we investigated younger and older individuals with persistent CMV infection, who developed large expansions of CMV-specific CD4+ T cells within certain Vβ families. In the representative individual referred to in Figure Figure5A,5A, CMV-specific CD4+ T cells, identified by IFN-γ secretion after stimulation with CMV lysate (32), were largely restricted to the Vβ2 family (Figure (Figure5A).5A). This CD4+Vβ2+ T cell population showed low Bcl-2 expression (Figure (Figure5B),5B), was largely CD27CD28 (Figure (Figure5C),5C), and had short telomeres (Figure (Figure5D)5D) relative to other less-expanded Vβ families. This is consistent with previous observations that CMV-specific CD4+ T cells in both younger and older subjects were highly differentiated and susceptible to apoptosis (32). This strongly suggested that the CD4+Vβ2+ expansion in this subject was likely to be induced by extensively differentiated CMV-specific CD4+ T cells (15, 32) rather than selective Vβ family usage that may be genetically determined in this individual. We found that 1.5%–2.0% of the total CD4+ T cell population in this subject was CD4+CD45RO+Foxp3+CD25hi, a value in the same range as that of the cohort of individuals previously tested (Figure (Figure1A).1A). The expanded CD4+Vβ2+ T cell population was not only found in the CD4+CD25 and CD4+CD25int cells but was also highly represented in the CD4+CD45RO+CD25hi population and constituted 15% of these cells (Figure (Figure5E).5E). Furthermore, when we examined Foxp3CD4+ and Foxp3+CD4+ T cells from this individual, we found that the expanded Vβ2 population was found in both fractions (Figure (Figure5F).5F). This further indicates that expansions of memory/effector CD4+CD45RO+Foxp3CD25 and CD4+CD45RO+Foxp3+CD25hi Tregs are antigen-driven and related and that they arise in tandem.

Figure 5
Antigen-driven expansion of CD4+ T cells is associated with concomitant expansion within the CD4+ CD45RO+ Foxp3+ CD25hi Treg population. ...


The key observations of this study are, first, that human CD4+CD25hiFoxp3+ Tregs divide rapidly in vivo but have limited capacity for extensive self-renewal, and second, that it is very likely that these cells are continuously recruited from the memory CD4+ T cell pool. These observations are entirely compatible with recent elegant studies in the mouse, in which it has been shown that CD4+CD25hiFoxp3+ T cells can be induced by antigen in the periphery (810). However, the considerably different life span of the mouse and human dictate that the constraints imposed on the maintenance of these cells by continuous proliferation in both species will be very different. These differences have to be taken into consideration when attempts are made to manipulate CD4+CD25hiFoxp3+ Tregs for therapeutic purposes. We find that human CD4+CD25hiFoxp3+ T cells are susceptible to apoptosis through reduced Bcl-2 expression and have limited replicative capacity resulting from poor telomerase inducibility and extensive telomere erosion. These constraints, together with thymic involution, could be expected to result in a reduction in numbers of CD4+CD25hi cells with age. Contrary to this, we have confirmed and extended previous reports that proportions of CD4+CD25hi Tregs are actually increased in older subjects (34).

The concept that there is inherent plasticity in the functional repertoire of CD4+ T cells is highlighted by the ability to induce different Th1 and Th2 cytokine secretion profiles in the presence of exogenous cytokines (35). The results presented here suggest that there are other points in the CD4+ T cell differentiation pathway where exogenous signals can induce these cells to differentiate into regulatory populations. It has been shown that CD4+CD25hiFoxp3+ Tregs can be induced from CD4+CD25Foxp3 T cell populations in the presence of TGF-β in vivo (9, 36, 37). In addition to CD4+CD25hi T cells, other regulatory populations, such as IL-10–producing Tr1 (38) and TGF-β–producing Th3 cells (39) can also be induced from naive and/or memory CD25 T cells under specific conditions. This suggests that active mediators may direct the development of Treg populations in cellular microenvironments. In addition, CD4+ Tregs can also be induced by specific surface receptor triggering, and we and others have shown previously that the induction of anergy in antigen-specific CD4+ T cell populations induces these cells to become suppressive in vitro (40, 41) and in vivo (42). We extended these observations recently by showing that human memory CD4+ T cells isolated from a site of antigenic rechallenge in the skin in vivo (31) could be anergized in vitro and that this induced high levels of Foxp3 expression and suppressive activity in these cells (data not shown). This is also supported by studies that suggest that some human responsive CD4+CD25 T cells undergo a transition into CD4+CD25hi Tregs as a natural event after specific activation in vitro (43). Collectively, these data indicate that the perception of the extent to which the functional CD4+ T cell repertoire is flexible should be extended to include the ability of exogenous cues to induce suppressive activity in these cells. In addition, since CD4+CD25hiFoxp3+ T cells have been found consistently to have a highly differentiated phenotype, it is likely that the regulatory cues only have an effect on CD4+ T cells that have been activated to proliferate extensively.

Two unresolved questions are whether naturally occurring thymic-derived CD4+CD25hi T cells are identical to CD4+CD25hi T cells that are induced from primed/memory CD4+CD25 T cells in the periphery and whether all CD4+Foxp3+CD25hi Tregs that are induced in different ways are exactly the same. Indeed, the relationship between these and other regulatory CD4+ populations, such as those that secrete IL-10 (38, 44), remains to be clarified. However, since CD4+Foxp3+CD25hi Tregs can be induced by cues that may be present in tissue microenvironments, some of the defects in CD4+Foxp3+CD25hi Tregs that have been reported in certain diseases (4548) may be due to aberrant induction and not dysfunction of these cells per se. Furthermore, since the human CD4+CD25hiFoxp3+ T cell population is susceptible to apoptosis, infusion of these cells into individuals with various diseases to modulate disease activity may only elicit short-term benefit as they may be cleared rapidly in vivo. In this context, survival factors, such as IL-2, that have been shown to be essential for the generation of CD4+CD25hiFoxp3+ T cells may be effective in part through their ability to counteract the susceptibility of these cells to apoptosis (4951). In contrast, the proximity of CD4+CD25hiFoxp3+ T cells to replicative senescence may be of therapeutic benefit since their capacity for continuous proliferation will be limited in vivo. This would preclude the development of generalized immunosuppression arising from the potential transfer of suppressive activity to other cells (52).

In summary, we show that rapid peripheral turnover is responsible for the maintenance of the human CD4+CD25hiFoxp3+ T cell population during aging. Our observations suggest a model whereby the context of stimulation of a memory CD4+ T cell population in peripheral tissues leads to the generation of CD4+ Tregs, which are relatively short-lived. This would promote immune resolution yet permit future responses to subsequent antigenic challenge. Such a model links the extent of regulation induced during an immune response to the level of antigenic stimulation. Such linkage may be part of the mechanism whereby peripheral tolerance to persistent antigens is achieved (32). Furthermore, these data highlight the potential of persistent infections with viruses, such as CMV, that drive the continuous lifelong differentiation of specific CD4+ T cells (32) to induce the generation of CD4+CD25hiFoxp3+ T cells. Such processes may contribute to the immune defect that is well documented in older subjects (15, 53).



Healthy younger (<35 years) and older (>70 years) individuals were recruited from volunteers at University College London and St. George’s, University of London. In total, 27 younger (mean age 27, range 21–34) and 31 older volunteers (mean age 78, range 70–90) were recruited into the study. Subjects diagnosed with active disease or using any medication thought to affect immune function were excluded. For the glucose labeling and turnover analysis, 4 younger (mean age 25, range 21–31; 2 males/2 females) and 4 older subjects (mean age 81, range 76–85; 3 males/1 female) were recruited for the study. All provided written informed consent to protocols approved by local ethics committees, and study procedures were performed in accordance with the principles of the World Medical Association Declaration of Helsinki.

Magnetic purification of the T cell subsets from peripheral blood.

PBMCs were isolated by density centrifugation on Ficoll-Paque PLUS (Amersham Biosciences), and CD4+ T cells were isolated by negative selection using the CD4 isolation kit for magnetic separation (Miltenyi Biotec) according to the manufacturer’s instructions. CD4+ T cells were incubated with mouse anti-human CD25 MicroBeads (2 μl/107 cells; Miltenyi Biotec) and separated into CD4+CD25+ and CD4+CD25 T cells on a positive selection column (LS; Miltenyi Biotec). CD4+CD25 fraction was then reincubated with CD25 MicroBeads (20 μl/107 cells) and run over a fresh LS column. This separation resulted in less than 1% CD25+ cells in the CD4+CD25 fraction and greater than 95% purity in the CD25+ T cell subset.

FACS sorting of T cell subsets from peripheral blood.

PBMCs were isolated from peripheral blood as before, and CD4 T cells were purified by magnetic separation as above. Purified CD4+ T cells were stained with a mixture of antibodies consisting of anti-CD4–PerCP (PerCP, peridinin-chlorophyll–protein complex) (BD), anti-CD45RA-FITC (BD Biosciences — Pharmingen), and anti-CD25–PE (Dako) for 30 minutes on ice, washed in PBS/2% BSA, filtered, and sorted on a MoFlo flow cytometer (Dako). Gates were set so that purified CD4+CD25hi cells represented the brightest 2% of total CD4 population; both CD4+CD25hi and CD4+CD25 populations were collected from the CD45RA fraction and were therefore designated as CD45RO+ for clarity (Supplemental Figure 4). Naive CD4+CD45RA+ cells were also collected in some experiments.

Phenotype analysis and telomere measurement.

For phenotypic analysis, PBMCs were stained with a combination of antibodies including anti-CD4–PerCP, anti-CD25–PE, anti-CD45RB–FITC, anti-CD45RA–APC, anti–Bcl-2–FITC, and anti-CTLA4–APC. TCR Vβ repertoire usage was investigated using the IOTest Beta Mark kit according to the manufacturer’s recommendations (Beckman Coulter). Gates were set so that the CD4+CD25 population was based on the isotype control while the CD25hi population was determined relative to the low intensity of CD25 staining found on non-CD4 T cells. The population between these 2 gates was considered CD25int. Cells were analyzed using a FACSCalibur with CellQuest software, version 3.3 (BD).

Foxp3 expression was analyzed by FACS according to the protocol described by Roncador et al. (54). In brief, fresh PBMCs were washed in PBS, fixed in 1 ml of PBS with 1% paraformaldehyde and 0.05% Tween, and kept overnight at 4°C. Cells were treated twice with 0.5 ml of DNase at 100 Kunitz/ml according to manufacturer’s instructions (Sigma-Aldrich). Cells were incubated with mouse anti-human Foxp3 (clone150D/E4, a kind gift from Alison Banham, University of Oxford, Oxford, United Kingdom) for 1 hour at room temperature and washed with FACS buffer (PBS, 3% FCS, 0.5% Tween-20, and 0.05% azide). Foxp3 staining was detected by staining with Alexa Fluor 488 goat anti-mouse IgG (Molecular Probes; Invitrogen). Cell-surface staining was performed using anti-CD4–PerCP (BD) and anti-CD25–PE (Dako) for 20 minutes at room temperature.

For telomere length measurement, we used a 3-color flow-FISH technique (31). PBMCs were stained with anti-CD4–biotin (Beckman Coulter) followed by streptavidin-Cy3 (Cedarlane Laboratories Ltd.) and either anti-CD25–FITC or anti-CD45RO–FITC. Following fixation, cells were hybridized with 0.75 μg/ml of the peptide nucleic acid telomeric (C3TA2)3 probe conjugated to Cy5. Samples were heated for 10 minutes at 82°C, rapidly cooled on ice, and hybridized for 1 hour at room temperature in the dark. Samples were then washed and analyzed immediately by flow cytometry. All staining was performed in triplicate, and telomere lengths for total CD4, CD4+CD45RO+, and CD4+CD25hi subsets were obtained.

Phenotypic analysis of CMV-specific CD4+ cells.

PBMCs from CMV-seropositive donors were stimulated with CMV lysate overnight in the presence of 5 μg/ml brefeldin A. Cells were stained with CD4-PerCP and a range of Vβ antibodies from the IOTest Beta Mark kit, fixed, permeabilized, and stained with IFN-γ APCs. For phenotypic analysis of a particular Vβ, PBMCs were costained with the appropriate Vβ antibody, then either stained with CD27 and CD28, stained with Bcl-2, or hybridized with the telomere probe.

Proliferation and suppression assays.

Purified CD4 T cell subsets (5 × 104/well) were stimulated in triplicate wells with Ag (purified protein derivative or tetanus toxoid) and irradiated (40 Gy) autologous PBMCs as APCs (5 × 104/well) in 96-well round-bottom plates (Nunc). For suppression assays, 5 × 104 CD4+CD25 T cells were stimulated in the absence or presence of CD4+CD25+ T cells at responder/suppressor ratios of 1:0 and 1:1. As a control, an equal number of CD4+CD25 cells were added. Cells were cultured in RPMI-1640 medium (Invitrogen) supplemented with 1% penicillin/streptomycin, 1% l-glutamine, and 10% human AB serum (all Sigma-Aldrich) for 6–7 days. During the last 18 hours of culture, 3H-thymidine was added at 1 μCi/well. For polyclonal stimulation, anti-CD3 (OKT3) was used and was immobilized on flat-bottom 96-well plates at 0.1 or 0.5 μg/ml in the presence of irradiated autologous PBMCs as APCs. Proliferation was measured at 72 hours. Proliferative responses are expressed as mean 3H-thymidine incorporation (cpm) of triplicate wells ± SEM.

Assessment of clonality by heteroduplex analysis.

The clonal composition of purified CD4+RO+CD25 and CD4+RO+CD25hi T cell populations FACS sorted from PBMCs was determined by comparing the CDR3 region of different TCR Vβ families. PCR was performed on Vβs 1–24, including Vβ 5.1 and 5.2 and Vβ 13.1 and 13.2 families, using specific Vβ and common Cβ primers, as described extensively elsewhere (33). Each sample PCR was mixed with Vβ-matched carrier DNA, denatured, and reannealed. The product was run on a 12% polyacrylamide gel, which was then blotted and hybridized with a probe to the external Cβ region of the carrier. The probe was detected using anti-digoxigenin–alkaline phosphatase Fab fragments and CDP-Star substrate (Roche Diagnostics). Identical clones within CD4+ T cell populations were detected as heteroduplex bands with identical migration patterns (33). The presence of heteroduplexes has previously been shown to relate to the presence of an expanded clone as determined by the sequencing of PCR product (33).

Telomerase activity measurement.

Telomerase activity was measured using the telomeric repeat amplification protocol (TRAP, TRAPeze Telomerase Detection Kit; Intergen Company). In brief, telomerase present in a test cell extract extends a template with telomeric repeats and, following PCR amplification, generates a ladder of products with 6-bp increments starting at 50 nucleotides. FACS-sorted CD4+CD25hi and CD4+CD25 were stimulated in vitro with anti-CD3/anti-CD28–coated beads for 4 days. Samples were collected by snap freezing of cells from in vitro cultures. Absolute numbers of CD4+Ki67+ cells in each sample were enumerated using TruCOUNT Tubes (BD) and Ki67 analysis. PCR was performed with samples adjusted to 500 Ki67+ T cells per reaction. The negative control contains the PCR mix without cell extract, and the positive control contains an extract of a telomerase-positive tumor cell line. TSR8 is a telomeric template that is used as a PCR control.

Glucose labeling and turnover analysis.

Subjects received deuterium-labeled glucose (6,6-D2-glucose, 0.6 g/kg; Cambridge Isotope Laboratories Inc.) in half-hourly aliquots over a 10-hour period during which subjects received small, low-energy meals at 2.5-hour intervals as previously described (55). In order to assess constancy of labeling, blood samples were taken approximately every 2 hours during glucose administration, and plasma glucose enrichment was measured following derivatization to the aldononitrile acetate derivative by gas chromatography mass spectrometry (monitoring ions m/z 328 and 330 by SIM, Agilent 5973/6890, HP-225 column; Agilent Technologies). The mean enrichment for the labeling period was calculated form the area under the curve.

For estimation of deuterium enrichment in cellular DNA, 50 ml heparinized blood samples were taken at 3, 4, 10, and 21 days after labeling. Enrichment of deuterium in DNA from purified cell populations was assayed essentially as described previously (56). In brief, cellular DNA was extracted and digested enzymatically to deoxynucleosides. The deoxyadenosine fraction was taken as a representative deoxynucleoside and analyzed as the aldononitrile acetate derivative in triplicate by gas chromatography mass spectrometry as above (except monitoring ions 198 and 200) (56). The minimum quantity of cells that can be analyzed in this way is 2 × 105, equivalent to about 1 mg of DNA; typical retrieval amounts for CD4+CD45RACD25hi T cells, the smallest population that was sorted from 50 mls of blood, were 3 × 105 cells.

Results were expressed as the fraction of labeled cells (F), expressed as equivalents of a 1-day labeling period, present at each sampling point, where F is equal to the ratio of the enrichment of deuterium in DNA (E) to the precursor enrichment (b) (taken as mean plasma glucose corrected for intracellular dilution by a factor of 0.65) (24), so that F = E/b. The magnitude of the peak value for F represents a measure of the cellular proliferation rate.

Data was modeled as previously described (56) (SigmaPlot, version 8.02; SPSS) to estimate the rate of proliferation during labeling, p, and the rate of loss of labeled cells, d*, by nonlinear least squares regression to the following formulas: F(t) = p/d*(1 – e–d*t), where t ≤ τ during the labeling period, and F(t) = p/d*(1 – e–d*τ)e–d*(t – τ), where t > τ during the labeling period, and where t is time and τ is the length of the labeling period. In this model, constancy of pool size has been assumed, but no assumption of equality between p and d* has been made: p represents the average proliferation rate of the whole population whereas d* refers only to labeled cells (i.e., cells which divided during the labeling period). For a kinetically heterogeneous population, even one at steady state, these 2 rates will not be the same, as discussed (2, 56). Proliferation expressed as T2 and disappearance as half-life (t1/2) were calculated as ln 2/p and ln 2/d*, respectively. Statistical comparisons between cell types were made by ANOVA with post hoc Tukey-Kramer test and between paired data from CD25hi and CD25 cell populations by 2-tailed, paired Student’s t test.


Statistical significance was evaluated using a Student’s 2-tailed t test and, where appropriate, a 2-tailed, paired Student’s t test. Differences were considered significant at P < 0.05.


We thank Alison Banham for the generous gift of Foxp3 antibody, Katie Lacy for organizing blood sample collection, Becca Asquith for advice on modeling, and Andrew Worth for expert cell sorting. This work was supported by grants from Biotechnology and Biological Sciences Research Council (to M. Vukmanovic-Stejic), Dermatrust (to A. McQuaid), Research into Ageing (to J.E. Cook and J.E. Masters), and The Henry Smith Charity (to J.M. Fletcher). Y. Zhang received support from the Medical Research Council and the Edward Jenner Institute for Vaccine Research.


Nonstandard abbreviations used: d*, disappearance rate constant; T2, doubling time; p, proliferation rate constant; PerCP, peridinin-chlorophyll–protein complex.

Conflict of interest: The authors have declared that no conflict of interest exists.

Citation for this article: J. Clin. Invest. 116:2423–2433 (2006). doi:10.1172/JCI28941.

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