• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of clinexpimmunolLink to Publisher's site
Clin Exp Immunol. Feb 1992; 87(2): 256–260.
PMCID: PMC1554259

Antibodies against C1q in anti-glomerular basement membrane nephritis.

Abstract

The prevalence of antibodies against the collagen-like region of the subcomponent of the first component of complement, C1q, was investigated in 11 patients with anti-glomerular basement membrane (GBM) nephritis. Anti-C1q antibodies (anti-C1qAb) were detected in seven patients. IgG anti-C1qAb were found in four and IgA anti-C1qAb in five patients. During follow up of the patients a relationship was observed between the levels of IgG anti-C1qAb and the levels of anti-GBM antibodies (anti-GBMAb). Gelfiltration experiments indicated that both IgG anti-C1qAb as well as IgG anti-GBMAb were monomeric and that binding also occurred with the F(ab')2 fragments of the antibodies. Although anti-C1qAb and anti-GBMAb are both directed against a collagen-like structure, it was demonstrated by means of inhibition experiments that anti-C1qAb and anti-GBMAb are directed against different antigenic sites. Comparison of patients with anti-GBM nephritis with and without anti-C1qAb revealed that there were no differences in disease activity or disease severity. Therefore, the results of this study suggest that anti-C1qAb do not play a direct pathogenetic role in anti-GBM nephritis.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (823K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • McPhaul JJ, Jr, Dixon FJ. Characterization of human anti-glomerular basement membrane antibodies eluted from glomerulonephritic kidneys. J Clin Invest. 1970 Feb;49(2):308–317. [PMC free article] [PubMed]
  • McIntosh RM, Copack P, Chernack WB, Griswold WR, Weil R, 3rd, Koss MN. The human choroid plexus and autoimmune nephritis. Arch Pathol. 1975 Jan;99(1):48–50. [PubMed]
  • Wieslander J, Barr JF, Butkowski RJ, Edwards SJ, Bygren P, Heinegård D, Hudson BG. Goodpasture antigen of the glomerular basement membrane: localization to noncollagenous regions of type IV collagen. Proc Natl Acad Sci U S A. 1984 Jun;81(12):3838–3842. [PMC free article] [PubMed]
  • Hudson BG, Wieslander J, Wisdom BJ, Jr, Noelken ME. Goodpasture syndrome: molecular architecture and function of basement membrane antigen. Lab Invest. 1989 Sep;61(3):256–269. [PubMed]
  • Tenner AJ, Lesavre PH, Cooper NR. Purification and radiolabeling of human C1q. J Immunol. 1981 Aug;127(2):648–653. [PubMed]
  • Kolb WP, Kolb LM, Podack ER. C1q: isolation from human serum in high yield by affinity chromatography and development of a highly sensitive hemolytic assay. J Immunol. 1979 May;122(5):2103–2111. [PubMed]
  • Siegert CE, Daha MR, van der Voort EA, Breedveld FC. IgG and IgA antibodies to the collagen-like region of C1q in rheumatoid vasculitis. Arthritis Rheum. 1990 Nov;33(11):1646–1654. [PubMed]
  • Westberg NG, Michael AF. Human glomerular basement membrane. Preparation and composition. Biochemistry. 1970 Sep 15;9(19):3837–3846. [PubMed]
  • Fish AJ, Kleppel M, Jeraj K, Michael AF. Enzyme immunoassay of anti-glomerular basement membrane antibodies. J Lab Clin Med. 1985 Jun;105(6):700–705. [PubMed]
  • Strife CF, Leahy AE, West CD. Antibody to a cryptic, solid phase C1Q antigen in membranoproliferative nephritis. Kidney Int. 1989 Mar;35(3):836–842. [PubMed]
  • Uwatoko S, Aotsuka S, Okawa M, Egusa Y, Yokohari R, Aizawa C, Suzuki K. Characterization of C1q-binding IgG complexes in systemic lupus erythematosus. Clin Immunol Immunopathol. 1984 Jan;30(1):104–116. [PubMed]
  • Uwatoko S, Aotsuka S, Okawa M, Egusa Y, Yokohari R, Aizawa C, Suzuki K. C1q solid-phase radioimmunoassay: evidence for detection of antibody directed against the collagen-like region of C1q in sera from patients with systemic lupus erythematosus. Clin Exp Immunol. 1987 Jul;69(1):98–106. [PMC free article] [PubMed]
  • Uwatoko S, Mannik M. Low-molecular weight C1q-binding immunoglobulin G in patients with systemic lupus erythematosus consists of autoantibodies to the collagen-like region of C1q. J Clin Invest. 1988 Sep;82(3):816–824. [PMC free article] [PubMed]
  • Antes U, Heinz HP, Loos M. Evidence for the presence of autoantibodies to the collagen-like portion of C1q in systemic lupus erythematosus. Arthritis Rheum. 1988 Apr;31(4):457–464. [PubMed]
  • Wener MH, Uwatoko S, Mannik M. Antibodies to the collagen-like region of C1q in sera of patients with autoimmune rheumatic diseases. Arthritis Rheum. 1989 May;32(5):544–551. [PubMed]
  • Daha MR, Klar N, Hoekzema R, van Es LA. Enhanced Ig production by human peripheral lymphocytes induced by aggregated C1q. J Immunol. 1990 Feb 15;144(4):1227–1232. [PubMed]
  • Siegert C, Daha M, Westedt ML, van der Voort E, Breedveld F. IgG autoantibodies against C1q are correlated with nephritis, hypocomplementemia, and dsDNA antibodies in systemic lupus erythematosus. J Rheumatol. 1991 Feb;18(2):230–234. [PubMed]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

  • MedGen
    MedGen
    Related information in MedGen
  • PubMed
    PubMed
    PubMed citations for these articles
  • Substance
    Substance
    PubChem Substance links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...