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Journal List > Clin Exp Immunol > v.15(4); Dec 1973

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Clin Exp Immunol. 1973 December; 15(4): 613–622.
PMCID: PMC1553964
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Separation and characterization of cardiac antigen proteins
U. C. Chaturvedi, J. W. Davies, and T. H. Flewett
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Abstract
An attempt has been made to purify and characterize the protein antigens related to autoimmune carditis in the heart extract of albino rats by ammonium sulphate fractionation, molecular sieve gel filtration and polyacrylamide gel electrophoresis. The heart extract of albino rat at pH 7·0 contains at least nine proteins out of which three are antigenic in rabbits and have been termed as CA I, II and III. These antigens can be purified by ammonium sulphate fractional precipitation followed by Sephadex G-100 gel filtration. Their molecular weights were 6·6 (±2) × 104, 2·8 (±0·85) × 104, 4·8 (±1·5) × 104 Daltons. A fourth antigen (CA IV) detected is probably polysaccharide.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
  • Chaturvedi UC, Mehrotra RM. Experimentally produced immunologic cardiac lesions. Indian J Med Res. 1967 Jan;55(1):21–28. [PubMed]
  • Chaturvedi UC, Gupta RK, Mehrotra RM. Antigenicity and immunopathogenicity of the chemical fractions of heart. Indian J Med Res. 1971 Aug;59(8):1237–1244. [PubMed]
  • Chaturvedi UC, Mathur A, Gupta RK, Mehrotra RM. Immunopathological studies in pericardiotomised rats. J Pathol. 1973 Apr;109(4):345–350. [PubMed]
  • DAVIES AM, LAUFER A, GERY I, ROSENMANN E. ORGAN SPECIFICITY OF THE HEART. 3. CIRCULATING ANTIBODIES AND IMMUNOPATHOLOGICAL LESIONS IN EXPERIMENTAL ANIMALS. Arch Pathol. 1964 Oct;78:369–376. [PubMed]
  • Espinosa E, Kaplan MH. Antigenic analysis of human heart tissue. Further characterization of an organ-specific antigen of heart tissue. J Immunol. 1971 Mar;106(3):611–618. [PubMed]
  • KAPLAN MH, CRAIG JM. IMMUNOLOGIC STUDIES OF HEART TISSUE. VI. CARDIAC LESIONS IN RABBITS ASSOCIATED WITH AUTOANTIBODIES TO HEART INDUCED BY IMMUNIZATION WITH HETEROLOGOUS HEART. J Immunol. 1963 May;90:725–733. [PubMed]
  • ROBINSON J, BRIGDEN W. IMMUNOLOGICAL STUDIES IN THE POST-CARDIOTOMY SYNDROME. Br Med J. 1963 Sep 21;2(5359):706–709. [PubMed]
  • Sharma SS, Kumar S, Kunwar KB, Mehrotra RM, Chaturvedi UC, Mitra MK. Anticardiac antibodies in ischaemic heart disease. J Assoc Physicians India. 1970 Jul;18(7):595–601. [PubMed]
  • Weber K, Osborn M. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 1969 Aug 25;244(16):4406–4412. [PubMed]
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