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Clin Exp Immunol. Jul 1989; 77(1): 31–36.
PMCID: PMC1541934

Heterogeneity of B cell responsiveness to interleukin 4, interleukin 6 and low molecular weight B cell growth factor in discrete stages of B cell activation in patients with systemic lupus erythematosus.

Abstract

To investigate the differential stages of B cell activation in patients with systemic lupus erythematosus (SLE), the effects of recombinant interleukin-4 (rIL-4) interleukin-6 (rIL-6), and low mol. wt BCGF (1-BCGF) on B cell proliferation and differentiation were evaluated. In a co-stimulatory assay with anti-IgM, proliferative response to rIL-4 and 1-BCGF showed no significant differences between SLE patients and healthy controls. In a restimulation assay, after pre-activation with anti-IgM for 3 days, proliferative response to rIL-4 and 1-BCGF was significantly decreased in SLE patients compared with normal healthy controls (P less than 0.01). With regard to rIL-6 induced B cell differentiation, SAC-pre-activated low density, large B cells from both SLE patients and healthy controls differentiated well, whereas high density B cells did not differentiate at all. Of particular interest, low density B cells from active patients directly differentiated into Ig secreting cells in response to rIL-6 without SAC activation. These data indicate that heterogeneity of B cell responsiveness to B cell-tropic interleukins resulted from the discrete stages of B cell activation in SLE.

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Selected References

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  • Budman DR, Merchant EB, Steinberg AD, Doft B, Gershwin ME, Lizzio E, Reeves JP. Increased spontaneous activity of antibody-forming cells in the peripheral blood of patients with active SLE. Arthritis Rheum. 1977 Apr;20(3):829–833. [PubMed]
  • Clutterbuck E, Shields JG, Gordon J, Smith SH, Boyd A, Callard RE, Campbell HD, Young IG, Sanderson CJ. Recombinant human interleukin 5 is an eosinophil differentiation factor but has no activity in standard human B cell growth factor assays. Eur J Immunol. 1987 Dec;17(12):1743–1750. [PubMed]
  • Defrance T, Vanbervliet B, Aubry JP, Takebe Y, Arai N, Miyajima A, Yokota T, Lee F, Arai K, de Vries JE, et al. B cell growth-promoting activity of recombinant human interleukin 4. J Immunol. 1987 Aug 15;139(4):1135–1141. [PubMed]
  • Delfraissy JF, Wallon C, Vazquez A, Dugas B, Dormont J, Galanaud P. B cell hyperactivity in systemic lupus erythematosus: selectively enhanced responsiveness to a high molecular weight B cell growth factor. Eur J Immunol. 1986 Oct;16(10):1251–1256. [PubMed]
  • Delfraissy JF, Segond P, Galanaud P, Wallon C, Massias P, Dormont J. Depressed primary in vitro antibody response in untreated systemic lupus erythematosus. T helper cell defect and lack of defective suppressor cell function. J Clin Invest. 1980 Jul;66(1):141–148. [PMC free article] [PubMed]
  • Falkoff RJ, Zhu LP, Fauci AS. Separate signals for human B cell proliferation and differentiation in response to Staphylococcus aureus: evidence for a two-signal model of B cell activation. J Immunol. 1982 Jul;129(1):97–102. [PubMed]
  • Hirano T, Taga T, Nakano N, Yasukawa K, Kashiwamura S, Shimizu K, Nakajima K, Pyun KH, Kishimoto T. Purification to homogeneity and characterization of human B-cell differentiation factor (BCDF or BSFp-2). Proc Natl Acad Sci U S A. 1985 Aug;82(16):5490–5494. [PMC free article] [PubMed]
  • Hirose T, Hara M, Kitani A, Hirose W, Norioka K, Kawagoe M, Nakamura H. Abnormal production of and response to B-cell growth factor and B-cell differentiation factor in patients with systemic lupus erythematosus. Scand J Immunol. 1985 Feb;21(2):141–150. [PubMed]
  • Jasin HE, Ziff M. Immunoglobulin synthesis by peripheral blood cells in systemic lupus erythematosus. Arthritis Rheum. 1975 May-Jun;18(3):219–228. [PubMed]
  • Lee F, Yokota T, Otsuka T, Meyerson P, Villaret D, Coffman R, Mosmann T, Rennick D, Roehm N, Smith C, et al. Isolation and characterization of a mouse interleukin cDNA clone that expresses B-cell stimulatory factor 1 activities and T-cell- and mast-cell-stimulating activities. Proc Natl Acad Sci U S A. 1986 Apr;83(7):2061–2065. [PMC free article] [PubMed]
  • Martinez-Cordero E, Alcocer-Varela J, Alarcon-Segovia D. Stimulating and differentiation factors for human B lymphocytes in systemic lupus erythematosus. Clin Exp Immunol. 1986 Sep;65(3):598–604. [PMC free article] [PubMed]
  • Muraguchi A, Fauci AS. Proliferative responses of normal human B lymphocytes. Development of an assay system for human B cell growth factor (BCGF). J Immunol. 1982 Sep;129(3):1104–1108. [PubMed]
  • Nakagawa T, Nakagawa N, Goldstein H, Volkman DJ, Fauci AS. Demonstration that human B cells respond differently to interleukin 2 and B cell differentiation factor based on their stages of maturation. J Immunol. 1986 Nov 15;137(10):3175–3182. [PubMed]
  • Pelton BK, Denman AM. Spontaneous production of B cell growth factors by SLE lymphocytes. Clin Exp Immunol. 1987 Jan;67(1):159–166. [PMC free article] [PubMed]
  • Prud'Homme GJ, Park CL, Fieser TM, Kofler R, Dixon FJ, Theofilopoulos AN. Identification of a B cell differentiation factor(s) spontaneously produced by proliferating T cells in murine lupus strains of the lpr/lpr genotype. J Exp Med. 1983 Feb 1;157(2):730–742. [PMC free article] [PubMed]
  • Sakane T, Steinberg AD, Green I. Studies of immune functions of patients with systemic lupus erythematosus. I. Dysfunction of suppressor T-cell activity related to impaired generation of, rather than response to, suppressor cells. Arthritis Rheum. 1978 Jul-Aug;21(6):657–664. [PubMed]
  • Schur PH, Sandson J. Immunologic factors and clinical activity in systemic lupus erythematosus. N Engl J Med. 1968 Mar 7;278(10):533–538. [PubMed]
  • Sharma S, Mehta S, Morgan J, Maizel A. Molecular cloning and expression of a human B-cell growth factor gene in Escherichia coli. Science. 1987 Mar 20;235(4795):1489–1492. [PubMed]
  • Suzuki N, Ueda Y, Sakane T. Differential mechanism for differentiation into immunoglobulin-secreting cells in human resting B lymphocyte subsets isolated on the basis of cell density. J Clin Invest. 1988 Jan;81(1):261–269. [PMC free article] [PubMed]
  • Tan EM, Cohen AS, Fries JF, Masi AT, McShane DJ, Rothfield NF, Schaller JG, Talal N, Winchester RJ. The 1982 revised criteria for the classification of systemic lupus erythematosus. Arthritis Rheum. 1982 Nov;25(11):1271–1277. [PubMed]
  • Yokota T, Otsuka T, Mosmann T, Banchereau J, DeFrance T, Blanchard D, De Vries JE, Lee F, Arai K. Isolation and characterization of a human interleukin cDNA clone, homologous to mouse B-cell stimulatory factor 1, that expresses B-cell- and T-cell-stimulating activities. Proc Natl Acad Sci U S A. 1986 Aug;83(16):5894–5898. [PMC free article] [PubMed]

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