Figure 1
Targeted modification of the mouse Gulo gene. (A) The endogenous mouse Gulo locus. The thick horizontal bar indicates the phage clone containing exons 3–7, marked by black boxes. (B) The targeting vector. Arrows P1 and P2 are relative positions of primers used to screen embryonic stem cells for the targeting event. (C) The inactivated Gulo locus. [a] and [b] are probes used for Southern blottings. Sizes and
restriction enzymes of fragments diagnostic for the modification are indicated. B, BamHI; Bg, BglII; E, EcoRI; H, HindIII; N, NotI; S, SacI; X, XbaI. (D) Northern blot analysis of the liver RNA (20 mg) isolated from the livers of a male anda female of the three Gulo genotypes. The filter was hybridized with a Gulo probe (Upper), washed, andrehybridized with a glyceraldehyde-3-phosphate dehydrogenase gene (Gapdh) probe (Lower). The positions of 28S and18S RNA are marked.
restriction enzymes of fragments diagnostic for the modification are indicated. B, BamHI; Bg, BglII; E, EcoRI; H, HindIII; N, NotI; S, SacI; X, XbaI. (D) Northern blot analysis of the liver RNA (20 mg) isolated from the livers of a male anda female of the three Gulo genotypes. The filter was hybridized with a Gulo probe (Upper), washed, andrehybridized with a glyceraldehyde-3-phosphate dehydrogenase gene (Gapdh) probe (Lower). The positions of 28S and18S RNA are marked.