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Clin Exp Immunol. Sep 1976; 25(3): 480–486.
PMCID: PMC1541410

The Crithidia luciliae kinetoplast immunofluorescence test in systemic lupus erythematosus.


An immunofluorescence test for antibodies to native DNA, using the kinetoplast of Crithidia luciliae as substrate, has been assessed in comparison with the Farr precipitation technique, on a total of 395 sera from sixty-three patients with systemic lupus erythematosus, 185 other hospital patients and sixty healthy controls. The immunofluorescence test appears to have great specificity as a diagnostic test for SLE, though lacking the sensitivity of the Farr technique. Like the latter, it is altered by immunosuppressive treatment, and in patients with SLE nephritis on immunosuppression it does not show good correlation with activity of renal disease. Its specificity and simplicity nevertheless make it a valuable clinical test.

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Selected References

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  • Aarden LA, de Groot ER, Feltkamp TE. Immunology of DNA. III. Crithidia luciliae, a simple substrate for the determination of anti-dsDNA with the immunofluorescence technique. Ann N Y Acad Sci. 1975 Jun 30;254:505–515. [PubMed]
  • BONE GJ, STEINERT M. Isotopes incorporated in the nucleic acids of Trypanosoma mega. Nature. 1956 Aug 11;178(4528):308–309. [PubMed]
  • Edmonds JP, Johnson GD, Ansell BM, Holborow EJ. The value of tests for antibodies to DNA in monitoring the clinical course of SLE. A long-term study using the Farr test and the DNA counterimmunoelectrophoretic method. Clin Exp Immunol. 1975 Oct;22(1):9–15. [PMC free article] [PubMed]
  • Jokinen EJ, Julkunen H. DNA haemagglutination test in the diagnosis of systemic lupus erythematosus. Ann Rheum Dis. 1965 Sep;24(5):477–480. [PMC free article] [PubMed]
  • Pincus T, Schur PH, Rose JA, Decker JL, Talal N. Measurement of serum DNA-binding activity in systemic lupus erythematosus. N Engl J Med. 1969 Sep 25;281(13):701–705. [PubMed]
  • ROBBINS WC, HOLMAN HR, DEICHER H, KUNKEL HG. Complement fixation with cell nuclei and DNA in lupus erythematosus. Proc Soc Exp Biol Med. 1957 Dec;96(3):575–579. [PubMed]
  • SELIGMANN M. Mise en évidence dans le sérum de malades atteints de lupus erythémateux disséminé d'une substance déterminant une réaction de précipitation avec l'acide désoxyribonucléique. C R Hebd Seances Acad Sci. 1957 Jul 8;245(2):243–245. [PubMed]
  • Wold RT, Young FE, Tan EM, Farr RS. Deoxyribonucleic acid antibody: a method to detect its primary interaction with deoxyribonucleic acid. Science. 1968 Aug 23;161(3843):806–807. [PubMed]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology


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