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Plant Physiol. Jul 2000; 123(3): 795–806.
PMCID: PMC1539260

Positional Cloning in Arabidopsis. Why It Feels Good to Have a Genome Initiative Working for You



Positional (or map-based) cloning techniques are widely used to identify the protein products of genes defined by mutation. In Arabidopsis the information generated by the Genome Initiative is giving this approach a decisive boost. A wealth of sequence polymorphisms and molecular markers is now available and can be exploited for fine mapping with technically simple and robust polymerase chain reaction-based methods. As a result it has become possible to complete positional cloning projects in a short time and with relatively little effort.

Articles from Plant Physiology are provided here courtesy of American Society of Plant Biologists


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