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Clin Exp Immunol. Dec 1990; 82(3): 533–537.
PMCID: PMC1535487

Immunologic abnormality in NZB/W F1 mice. Thymus-independent expansion of B cells responding to interleukin-6.


We have previously reported that B cell abnormality in NZB/W F1 mice developed independently of thymus. Here we examined further whether B cells from NZB/W F1 mice respond to interleukin-6 (IL-6), a factor for terminal differentiation of B cells. When freshly isolated splenic B cells were incubated for 5 days in the presence of human IL-6, an increased production of both IgM and IgG, including anti-DNA antibody, was evident in NZB/W F1 mice; there was no increase in BALB/c mice. A magnitude of augmentation in IgG but not IgM production by IL-6 became more apparent in older NZB/W F1 mice. The increased immunoglobulin production seen with IL-6 was neutralized by treatment with rabbit anti-recombinant human IL-6 antibody. As B cells from athymic NZB/W F1 nude mice also responded to IL-6, it was suggested that B cells in NZB/W F1 mice differentiated into the IL-6-responding state in a thymus-independent manner. This B cell abnormality may be associated with the pathogenesis of autoimmune disease in NZB/W F1 mice.

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Selected References

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  • Christie JF, Gallagher G, Stimson WH. The human bladder carcinoma line T-24 secretes a human B-cell differentiation factor. Immunology. 1987 Mar;60(3):467–469. [PMC free article] [PubMed]
  • Hang L, Slack JH, Amundson C, Izui S, Theofilopoulos AN, Dixon FJ. Induction of murine autoimmune disease by chronic polyclonal B cell activation. J Exp Med. 1983 Mar 1;157(3):874–883. [PMC free article] [PubMed]
  • Herron LR, Coffman RL, Bond MW, Kotzin BL. Increased autoantibody production by NZB/NZW B cells in response to IL-5. J Immunol. 1988 Aug 1;141(3):842–848. [PubMed]
  • Hirano T, Matsuda T, Hosoi K, Okano A, Matsui H, Kishimoto T. Absence of antiviral activity in recombinant B cell stimulatory factor 2 (BSF-2). Immunol Lett. 1988 Jan;17(1):41–45. [PubMed]
  • Hirano T, Taga T, Nakano N, Yasukawa K, Kashiwamura S, Shimizu K, Nakajima K, Pyun KH, Kishimoto T. Purification to homogeneity and characterization of human B-cell differentiation factor (BCDF or BSFp-2). Proc Natl Acad Sci U S A. 1985 Aug;82(16):5490–5494. [PMC free article] [PubMed]
  • Hirano T, Taga T, Yasukawa K, Nakajima K, Nakano N, Takatsuki F, Shimizu M, Murashima A, Tsunasawa S, Sakiyama F, et al. Human B-cell differentiation factor defined by an anti-peptide antibody and its possible role in autoantibody production. Proc Natl Acad Sci U S A. 1987 Jan;84(1):228–231. [PMC free article] [PubMed]
  • Howie JB, Helyer BJ. The immunology and pathology of NZB mice. Adv Immunol. 1968;9:215–266. [PubMed]
  • Izui S, McConahey PJ, Dixon FJ. Increased spontaneous polyclonal activation of B lymphocytes in mice with spontaneous autoimmune disease. J Immunol. 1978 Dec;121(6):2213–2219. [PubMed]
  • Lambert PH, Dixon FJ. Pathogenesis of the glomerulonephritis of NZB/W mice. J Exp Med. 1968 Mar 1;127(3):507–522. [PMC free article] [PubMed]
  • Lehmann KR, Kotzin BL, Portanova JP, Santoro TJ. Interleukin 2 is a proliferative signal for B cells from autoimmune mice. Eur J Immunol. 1986 Sep;16(9):1105–1110. [PubMed]
  • Mihara M, Nakano T, Ohsugi Y. An immunomodulating anti-rheumatic drug, lobenzarit disodium (CCA): inhibition of polyclonal B-cell activation and prevention of autoimmune disease in MRL/Mp-lpr/lpr mice. Clin Immunol Immunopathol. 1987 Dec;45(3):366–374. [PubMed]
  • Mihara M, Ohsugi Y. The biological response modifier OK-432 (a streptococcal preparation) inhibits the development of autoimmune kidney disease in NZB/W F1 hybrid mice: possible involvement of tumor necrosis factor. Int Arch Allergy Appl Immunol. 1989;90(1):37–42. [PubMed]
  • Mihara M, Ohsugi Y, Saito K, Miyai T, Togashi M, Ono S, Murakami S, Dobashi K, Hirayama F, Hamaoka T. Immunologic abnormality in NZB/NZW F1 mice. Thymus-independent occurrence of B cell abnormality and requirement for T cells in the development of autoimmune disease, as evidenced by an analysis of the athymic nude individuals. J Immunol. 1988 Jul 1;141(1):85–90. [PubMed]
  • Ohsugi Y, Gershwin ME. Studies of congenitally immunologic mutant New Zealand mice. III. Growth of B lymphocyte clones in congenitally athymic (nude) and hereditarily asplenic (Dh/+) NZB mice: a primary B cell defect. J Immunol. 1979 Sep;123(3):1260–1265. [PubMed]
  • Prud'homme GJ, Balderas RS, Dixon FJ, Theofilopoulos AN. B cell dependence on and response to accessory signals in murine lupus strains. J Exp Med. 1983 Jun 1;157(6):1815–1827. [PMC free article] [PubMed]
  • McCoy KL, Kendrick L, Chused TM. Tolerance defects in New Zealand Black and New Zealand Black X New Zealand White F1 mice. J Immunol. 1986 Feb 15;136(4):1217–1222. [PubMed]
  • Steward MW, Hay FC. Changes in immunoglobulin class and subclass of anti-DNA antibodies with increasing age in N/ZBW F1 hybrid mice. Clin Exp Immunol. 1976 Nov;26(2):363–370. [PMC free article] [PubMed]
  • Theofilopoulos AN, Balderas RS, Gozes Y, Fidler JM, Liu FT, Ahmed A, Dixon FJ. Surface and functional characteristics of B cells from lupus-prone murine strains. Clin Immunol Immunopathol. 1982 May;23(2):224–244. [PubMed]

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