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Clin Exp Immunol. Jun 1995; 100(3): 499–505.
PMCID: PMC1534456

Autoantibody response to the Ro/La particle may predict outcome in neonatal lupus erythematosus.

Abstract

This study was undertaken to determine the role of antibodies against both recombinant Ro (r-Ro) and La (r-La) proteins and polypeptides derived from the recombinant La protein in predicting fetal and neonatal outcome in children at risk to develop neonatal lupus erythematosus (NLE). All sera were obtained in the perinatal period and quantitative ELISA assays were used. We collected 41 maternal sera within 2 months of delivery of a child with NLE (21 with congenital heart disease block (CHB) and 20 with dermatologic NLE) and 19 sera from anti-Ro and/or anti-La antibody-positive mothers with systemic lupus erythematosus (SLE) who delivered a child without NLE. All sera were tested for anti-r-La and anti-r-Ro antibodies by ELISA, and most sera were tested for antibodies directed against La polypeptides by immunoblot. We found significantly higher anti-r-La antibody levels in the sera from mothers of children with NLE compared with sera from mothers of unaffected children (0.67 +/- 0.43 versus 0.14 +/- 0.30; P < 0.0001). There was a statistically significant difference in the mean anti-r-La levels between the sera of mothers of children with CHB compared with dermatologic NLE (0.51 +/- 0.45 versus 0.83 +/- 0.37 respectively; P = 0.0091). When we examined antibodies directed against the recombinant 52-kD Ro protein, there was a statistically significant elevation of titres in the sera of mothers of NLE children (0.77 +/- 0.35) compared with non-NLE mothers (0.29 +/- 0.39; P < 0.0001). There was no difference in the r-Ro levels between mothers of children with dermatologic NLE compared with CHB (0.82 +/- 0.37 versus 0.71 +/- 0.74; P = 0.32). When we examined polypeptides derived from the recombinant La protein, the mean number of polypeptides recognized by sera from mothers of children with NLE was significantly higher than the mean number of polypeptides recognized by sera from mothers of unaffected children (5.1 +/- 0.54 versus 2.3 +/- 0.54 respectively; P < 0.001). More importantly, when we examined the individual polypeptides, we found that only sera from mothers of children with NLE and not from mothers of unaffected children recognized a polypeptide designated DD (30% versus 0%, respectively). These studies indicate that the autoantibody response to the Ro/La particle can differentiate sera from mothers of children with NLE and sera from mothers of unaffected children. Furthermore, there was a difference in the anti-La autoantibody response between mothers of children with CHB and dermatologic NLE.(ABSTRACT TRUNCATED AT 400 WORDS)

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  • Lee LA, Coulter S, Erner S, Chu H. Cardiac immunoglobulin deposition in congenital heart block associated with maternal anti-Ro autoantibodies. Am J Med. 1987 Oct;83(4):793–796. [PubMed]
  • Provost TT, Watson R, Gaither KK, Harley JB. The neonatal lupus erythematosus syndrome. J Rheumatol Suppl. 1987 Jun;14 (Suppl 13):199–205. [PubMed]
  • Lee LA, Norris DA, Weston WL. Neonatal lupus and the pathogenesis of cutaneous lupus. Pediatr Dermatol. 1986 Dec;3(6):491–497. [PubMed]
  • Reed BR, Lee LA, Harmon C, Wolfe R, Wiggins J, Peebles C, Weston WL. Autoantibodies to SS-A/Ro in infants with congenital heart block. J Pediatr. 1983 Dec;103(6):889–891. [PubMed]
  • Watson RM, Lane AT, Barnett NK, Bias WB, Arnett FC, Provost TT. Neonatal lupus erythematosus. A clinical, serological and immunogenetic study with review of the literature. Medicine (Baltimore) 1984 Nov;63(6):362–378. [PubMed]
  • Silverman E, Mamula M, Hardin JA, Laxer R. Importance of the immune response to the Ro/La particle in the development of congenital heart block and neonatal lupus erythematosus. J Rheumatol. 1991 Jan;18(1):120–124. [PubMed]
  • Buyon JP, Ben-Chetrit E, Karp S, Roubey RA, Pompeo L, Reeves WH, Tan EM, Winchester R. Acquired congenital heart block. Pattern of maternal antibody response to biochemically defined antigens of the SSA/Ro-SSB/La system in neonatal lupus. J Clin Invest. 1989 Aug;84(2):627–634. [PMC free article] [PubMed]
  • Buyon JP, Winchester RJ, Slade SG, Arnett F, Copel J, Friedman D, Lockshin MD. Identification of mothers at risk for congenital heart block and other neonatal lupus syndromes in their children. Comparison of enzyme-linked immunosorbent assay and immunoblot for measurement of anti-SS-A/Ro and anti-SS-B/La antibodies. Arthritis Rheum. 1993 Sep;36(9):1263–1273. [PubMed]
  • Meilof JF, Frohn-Mulder IM, Stewart PA, Szatmari A, Hess J, Veldhoven CH, Smeenk RJ, Swaak AJ. Maternal autoantibodies and congenital heart block: no evidence for the existence of a unique heart block-associated anti-Ro/SS-A autoantibody profile. Lupus. 1993 Aug;2(4):239–246. [PubMed]
  • Barakat S, Meyer O, Torterotot F, Youinou P, Briand JP, Kahn MF, Muller S. IgG antibodies from patients with primary Sjögren's syndrome and systemic lupus erythematosus recognize different epitopes in 60-kD SSA/Ro protein. Clin Exp Immunol. 1992 Jul;89(1):38–45. [PMC free article] [PubMed]
  • Ben-Chetrit E, Chan EK, Sullivan KF, Tan EM. A 52-kD protein is a novel component of the SS-A/Ro antigenic particle. J Exp Med. 1988 May 1;167(5):1560–1571. [PMC free article] [PubMed]
  • Bini P, Chu JL, Okolo C, Elkon K. Analysis of autoantibodies to recombinant La (SS-B) peptides in systemic lupus erythematosus and primary Sjogren's syndrome. J Clin Invest. 1990 Feb;85(2):325–333. [PMC free article] [PubMed]
  • McNeilage LJ, Umapathysivam K, Macmillan E, Guidolin A, Whittingham S, Gordon T. Definition of a discontinuous immunodominant epitope at the NH2 terminus of the La/SS-B ribonucleoprotein autoantigen. J Clin Invest. 1992 May;89(5):1652–1656. [PMC free article] [PubMed]
  • St Clair EW, Pisetsky DS, Reich CF, Keene JD. Analysis of autoantibody binding to different regions of the human La antigen expressed in recombinant fusion proteins. J Immunol. 1988 Dec 15;141(12):4173–4180. [PubMed]
  • Sturgess AD, Peterson MG, McNeilage LJ, Whittingham S, Coppel RL. Characteristics and epitope mapping of a cloned human autoantigen La. J Immunol. 1988 May 1;140(9):3212–3218. [PubMed]
  • Lee LA, Bias WB, Arnett FC, Jr, Huff JC, Norris DA, Harmon C, Provost TT, Weston WL. Immunogenetics of the neonatal lupus syndrome. Ann Intern Med. 1983 Nov;99(5):592–596. [PubMed]
  • Hamilton RG, Harley JB, Bias WB, Roebber M, Reichlin M, Hochberg MC, Arnett FC. Two Ro (SS-A) autoantibody responses in systemic lupus erythematosus. Correlation of HLA-DR/DQ specificities with quantitative expression of Ro (SS-A) autoantibody. Arthritis Rheum. 1988 Apr;31(4):496–505. [PubMed]
  • Hartung K, Ehrfeld H, Lakomek HJ, Coldewey R, Lang B, Krapf F, Müller R, Schendel D, Deicher H, Seelig HP. The genetic basis of Ro and La antibody formation in systemic lupus erythematosus. Results of a multicenter study. The SLE Study Group. Rheumatol Int. 1992;11(6):243–249. [PubMed]
  • Arnaiz-Villena A, Vazquez-Rodriguez JJ, Vicario JL, Lavilla P, Pascual D, Moreno F, Martinez-Laso J. Congenital heart block immunogenetics. Evidence of an additional role of HLA class III antigens and independence of Ro autoantibodies. Arthritis Rheum. 1989 Nov;32(11):1421–1426. [PubMed]
  • McCune AB, Weston WL, Lee LA. Maternal and fetal outcome in neonatal lupus erythematosus. Ann Intern Med. 1987 Apr;106(4):518–523. [PubMed]
  • Hardy JD, Solomon S, Banwell GS, Beach R, Wright V, Howard FM. Congenital complete heart block in the newborn associated with maternal systemic lupus erythematosus and other connective tissue disorders. Arch Dis Child. 1979 Jan;54(1):7–13. [PMC free article] [PubMed]
  • Waltuck J, Buyon JP. Autoantibody-associated congenital heart block: outcome in mothers and children. Ann Intern Med. 1994 Apr 1;120(7):544–551. [PubMed]
  • McCredie M, Celermajer J, Sholler G, Kelly D, Chivers T, Wang Y, Schrieber L. A case-control study of congenital heart block: association with maternal antibodies to Ro(SS-A) and La(SS-B). Br J Rheumatol. 1990 Feb;29(1):10–14. [PubMed]
  • Taylor PV, Taylor KF, Norman A, Griffiths S, Scott JS. Prevalence of maternal Ro (SS-A) and La (SS-B) autoantibodies in relation to congenital heart block. Br J Rheumatol. 1988 Apr;27(2):128–132. [PubMed]
  • Reichlin M, Wasicek CA. Clinical and biologic significance of antibodies to Ro/SSA. Hum Pathol. 1983 May;14(5):401–405. [PubMed]
  • Julkunen H, Kurki P, Kaaja R, Heikkilä R, Immonen I, Chan EK, Wallgren E, Friman C. Isolated congenital heart block. Long-term outcome of mothers and characterization of the immune response to SS-A/Ro and to SS-B/La. Arthritis Rheum. 1993 Nov;36(11):1588–1598. [PubMed]
  • Dugan EM, Tunnessen WW, Honig PJ, Watson RM. U1RNP antibody-positive neonatal lupus. A report of two cases with immunogenetic studies. Arch Dermatol. 1992 Nov;128(11):1490–1494. [PubMed]
  • Provost TT, Watson R, Gammon WR, Radowsky M, Harley JB, Reichlin M. The neonatal lupus syndrome associated with U1RNP (nRNP) antibodies. N Engl J Med. 1987 Apr 30;316(18):1135–1138. [PubMed]

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