• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of narLink to Publisher's site
Nucleic Acids Res. Nov 15, 1999; 27(22): e33.
PMCID: PMC148709

Normalization of array hybridization experiments in differential gene expression analysis.

Abstract

For detecting and confirming differentially expressed genes it is necessary to have a trustworthy reference. So called 'housekeeping genes' are frequently used for this purpose as internal standard. However, if the influence of new experimental conditions is to be analyzed it is not safe to assume a priori that the expression of these genes is not affected. Therefore two synthetic poly(A)-RNAs were generated by PCR and in vitro transcription. They were used as external standards for normalization of northern blots and cDNA arrays where non-regulated genes as internal reference were not available.

Full Text

The Full Text of this article is available as a PDF (197K).

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

Formats: