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Nucleic Acids Res. Mar 15, 1999; 27(6): 1558–1560.
PMCID: PMC148354

Amplification of cDNA ends based on template-switching effect and step-out PCR.

Abstract

A new method for amplifying cDNA ends is described which requires only first-strand cDNA synthesis and a single PCR to generate a correct product with very low or no background. The method can be successfully applied to total RNA as well as poly A+ RNA. The same first-strand cDNA can be used to amplify flanking sequences of any cDNA species present in the sample.

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