Logo of emborepLink to Publisher's site
EMBO Rep. 2006 Jun; 7(6): 628–634.
Published online 2006 Apr 28. doi:  10.1038/sj.embor.7400686
PMCID: PMC1479594
Scientific Report

Histone modification patterns associated with the human X chromosome


X inactivation is associated with chromosome-wide establishment of inactive chromatin. Although this is classically regarded as facultative heterochromatin that is uniform in nature, the exact distribution of associated epigenetic marks is not well defined. Here we have analysed histone modifications in human somatic cells within two selected regions of the X chromosome. Intergenic, coding and promoter regions are segregated into differentially marked chromatin. H3K27me3 is most prominent in intergenic and silenced coding regions, but is associated with some active coding regions as well. Histone H3/H4 acetylation and H3K4me3 are locally enriched at promoter regions but do not necessarily mark continuing transcription. Remarkably, H3K9me3 is predominant in coding regions of active genes, a phenomenon that is not restricted to the X chromosome. These results argue against the exclusiveness of individual marks to heterochromatin or euchromatin, but rather suggest that composite patterns of interdependent or mutually exclusive modifications together signal the gene expression status.

Keywords: facultative heterochromatin, histone methylation, X inactivation, escape


Facultative heterochromatin of the inactive X chromosome (Xi) is hallmarked by association of the Xi-specific non-coding transcript (XIST; Brown et al, 1991), histone deacetylation (Jeppesen & Turner, 1993), demethylation of histone H3 lysine 4 (H3K4; Boggs et al, 2002; Goto et al, 2002), dimethylation of histone H3 lysine 9 (H3K9me2; Heard et al, 2001), trimethylation of histone 3 lysine 27 (H3K27me3; Plath et al, 2003; Silva et al, 2003), mono-methylation of histone H4 lysine 20 (H4K20me1; Kohlmaier et al, 2004), methylation of promoter-containing CpG islands (Norris et al, 1991) and enrichment of the histone variant macroH2A (Costanzi & Pehrson, 1998). Facultative heterochromatin can be distinguished from constitutive heterochromatin by differential methylation of H3K9, H3K27 and H4K20. Facultative heterochromatin contains H3K27me3, H3K9me2 and H4K20me1, whereas constitutive heterochromatin contains H3K27me1, H3K9me3 and H4K20me3 (Peters et al, 2003; Kohlmaier et al, 2004; Schotta et al, 2004).

About 15% of human X-linked genes escape X inactivation to some extent, whereas another 10% show variable patterns of inactivation between individuals (Carrel & Willard, 2005). Although histone H3/H4 acetylation and H3K4 methylation have been correlated with escape from X inactivation, the exact distribution of these marks as well as H3K9/27 methylation is not well defined. Here we analysed two non-contiguous X-chromosomal regions using chromatin immunoprecipitation (ChIP). Our analyses show segregation of different types of chromatin at intergenic, coding and promoter regions. Intergenic and silenced coding regions have H3K27me3 as the most prominent mark. In contrast, promoter regions have high H3/H4 acetylation and H3K4me3, although their presence does not necessarily mark continuing transcription. Remarkably, we find that coding regions of active genes are enriched for H3K9me3. Analyses of several autosomal genes suggest that these patterns are general rather than X specific.


Gene expression analysis of selected genes

Because monoallelic expression of X-linked genes in XX cells complicates interpretation of ChIP-based histone modification and gene expression analysis, we first turned to hamster somatic hybrid cell lines containing the human Xi or active X chromosome (Xa), and subsequently to human XX and XY lymphoblastoid cell lines. We selected two chromosomal regions located on different arms of the X chromosome but outside the pseudoautosomal region, which is known to contain mostly genes that escape X-chromosome inactivation (XCI). In each case, we selected a single escape gene adjacent to X-inactivated genes, on the basis of expression analysis data described previously (Carrel & Willard, 2005).

Expression analysis using reverse transcription–quantitative PCR showed that within Xp22.22, PIGA (phosphatidylinositol N-acetylglucosaminyltransferase) was subject to XCI, whereas PIR (pirin) escaped XCI (Fig 1A). FIGF (c-Fos-induced growth factor/VEGF-D) and BMX (bone marrow non-receptor tyrosine kinase) were unexpressed in hybrids and lymphoblastoids and several other XX cell lines (Fig 1A; data not shown), which suggests that these genes are subject to cell-type-dependent repression. Within Xq13.1, RPS4X (ribosomal protein S4) escaped XCI, whereas HDAC8 (class I histone deacetylase) was subject to XCI (Fig 1B). CITED1 (CBP/p300-interacting transactivator 1) and PHKA1 (phosphorylase B kinase α) showed differential expression patterns: in the hybrids, CITED1 was subject to XCI, whereas PHKA1 escaped XCI; however, both genes were unexpressed in lymphoblastoid cells. CITED1 and PHKA1 were active in several other XX cell lines (data not shown), and their unexpressed state seemed to be specific for lymphoblastoid cells. This suggests that cell-type-dependent repression mechanisms may be dominant over the established XCI profiles. Although we did not discriminate between monoallelic or biallelic expression in XX lymphoblastoid cells, expression was probably monoallelic for PIGA and HDAC and bi-allelic for PIR and RPS4X, on the basis of their expression measured in hybrid cells. An expected twofold reduction between XX and XY cells was not evident for the XCI genes PIGA and HDAC8. We tentatively designated the selected genes as ‘subject to X inactivation' (HDAC8 and PIGA), ‘escaping from X inactivation' (RPS4X and PIR), ‘unexpressed' (FIGF and BMX) or ‘facultative' (CITED1 and PHKA1).

Figure 1
Complementary DNA expression analysis using reverse transcription–quantitative PCR. (A) Xp22.22. BMX, bone marrow non-receptor tyrosine kinase; FIGF, c-Fos-induced growth factor/VEGF-D; PIGA, phosphatidylinositol N-acetylglucosaminyltransferase; ...

Euchromatic histone modification patterns

To correlate gene activity with histone modification patterns, we carried out ChIP experiments using antibodies against di-acetylated histone H3 (lysines 9 and 14, H3ac), tetra-acetylated histone H4 (lysines 5, 8, 12 and 16, H4ac) and trimethylated histone H3 lysine 4 (H3K4me3). These modifications colocalized and were concentrated at promoter regions of the genes, where they probably constitute an active chromatin conformation (Fig 2). This active code generally extended over a region about 1 kb in length. Intergenic and coding regions were devoid of such active histone marks: signals decreased to background levels at a distance of over 1 kb upstream or downstream of the transcriptional start site. For PIGA, PIR, RPS4X and HDAC8, the presence of active histone marks correlated with their expression status; they were present only at active alleles. The correlation between active histone marks and gene activity was not evident for the facultative genes CITED1 and PHKA1; inactive alleles still contained active histone marks in hybrid Xi cells or lymphoblastoid XY and XX cells (Fig 2B). We conclude that H3/H4 acetylation and H3K4me3 colocalized at active promoter regions, except for the facultative genes CITED1 and PHKA1, which contain these marks independently of their expression status. No obvious differences in active code patterns were observed between hybrid cells and lymphoblastoid cells, and no escape-specific patterns of active histone modifications were evident.

Figure 2
ChIP analysis of histone modification patterns associated with X-linked genes of hamster–human hybrid cells (Xi and Xa) and human lymphoblastoid cells (XX and XY). (A) Xp22.22. (B) Xq13.1. Error bars represent standard deviation between at least ...

Heterochromatic histone modification patterns

To analyse histone modifications associated with heterochromatin, we carried out ChIP experiments for H3K9me2 and H3K9me3 and for H3K27me3. H3K9me2 has been associated with facultative heterochromatin and euchromatin (Peters et al, 2003; Rougeulle et al, 2004), H3K9me3 with constitutive heterochromatin, and H3K27me3 with the inactive X chromosome (Plath et al, 2003; Silva et al, 2003) and Polycomb-silenced genes (Cao et al, 2002; Kirmizis et al, 2004). The overall levels of H3K9me2 were low (Fig 2). Surprisingly, H3K9me3 was most prominent within coding regions of active alleles of PIGA, PIR (Fig 2A), RPS4X and HDAC8 (Fig 2B). The highest levels of H3K9me3 were found within the highly expressed RPS4X gene (up to 50% relative to GAPDH (glyceraldehyde-3-phosphate dehydrogenase); see Fig 1B), suggesting a correlation between the level of H3K9me3 and transcription elongation activity (see also Fig 4B).

Figure 4
Chromatin immunoprecipitation analysis of histone modification patterns associated with autosomal genes of human lymphoblastoid cells (XY). Legend as for Fig 2. Chromosome numbers are indicated. (A) Unexpressed genes (BDNF, brain-derived neuronal factor). ...

H3K27me3 covered intergenic, promoter and coding regions of inactive alleles, but was excluded from both promoter and coding regions of active alleles (Fig 2). However, such a pattern was not present at both the inactive and active CITED1 alleles in the hybrid cells, which showed promoter-restricted exclusion of H3K27me3 but maintenance of H3K27me3 in the coding region (Fig 2B). The distribution of H3K27me3 at the active PHKA1 allele in hybrid Xi cells resembled that of CITED1, although the PHKA1 promoter was not completely devoid of H3K27me3.

Active PIR alleles were enriched for H3K9me3 in their coding regions, although in lymphoblastoid XX cells H3K27me3 was also detected. Although this probably indicates that both PIR alleles are differentially marked, it is not clear whether the H3K27me3-marked allele represents an active or inactive gene, as H3K27me3 was also found within active coding regions (CITED1; Fig 2B).

FIGF was unexpressed in all cell lines tested, but its histone modification pattern was different from other unexpressed genes: FIGF lacked abundant H3K27me3 in hybrid Xi, Xa cells and lymphoblastoid XY cells, and instead its histone modification pattern seemed to be an extension of the pattern observed in the PIR active coding region (H3K9me3). The PIR–FIGF head-to-tail distance is less than 500 bp, and although PIR contains a poly(A) signal and yields polyadenylated messenger RNA (Wendler et al, 1997), the similarity between the PIR and FIGF modification patterns could be related to transcriptional read-through. Bi-cistronic mRNAs containing both PIR and FIGF have been observed (Wendler et al, 1997; Rocchigiani et al, 1998), and a corresponding bicistronic expressed sequence tag is present in the current database release (http://genome.ucsc.edu, May 2004).

In conclusion, whereas inactive alleles were marked by H3K27me3 in promoter and coding regions, H3K27me3 was excluded from these regions in active alleles. Instead, coding regions of active alleles contained H3K9me3 as the most prominent mark. H3K27me3 was maintained within coding regions of the active CITED1 and PHKA1 alleles. Furthermore, all intergenic regions were marked by H3K27me3.

H3K4me3 and H3K27me3 colocalization

Although we detected both the active mark H3K4me3 and the inactive mark H3K27me3 at promoter regions of unexpressed CITED1 and PHKA1 alleles, the detection of these marks could be caused by clonal differences in the cell populations rather than colocalization of these two opposing marks. To distinguish between these possibilities, we performed reChIP experiments in which different modifications were analysed consecutively on the same chromatin sample. Crosslinked chromatin from XY lymphoblastoid cells was subjected to a first-round ChIP, eluted and then subjected to a second-round ChIP. As expected, a first-round ChIP using anti-H3K9me3 or anti-H3K27me3 showed that CITED1 0.0 and PHKA1 0.0 contained H3K27me3 but not H3K9me3, whereas RPS4X +0.5 and PIGA +0.5 lacked both modifications (Fig 3). Next, anti-H3K9me3 and anti-H3K27me3 were used in the first-round ChIP, followed by a second-round ChIP using anti-H3K4me3. Although all of the analysed positions contained high H3K4me3 (Fig 2), this mark could be recovered only at CITED1 0.0 and PHKA1 0.0, and only after a first-round ChIP using anti-H3K27me3. RPS4X +0.5 and PIGA +0.5, which also have high H3K4me3, could not be recovered because these positions were devoid of H3K9me3 and H3K27me3. These results provide strong evidence for the simultaneous presence of H3K4me3 and H3K27me3 at CITED1 0.0 and PHKA1 0.0, and exclude the possibility that clonal differences within the cell population caused the detection of H3K4me3 and H3K27me3 at the same position.

Figure 3
ReChIP analysis of human XY lymphoblastoid cells. Two consecutive ChIP experiments were performed with antibodies against the indicated histone modifications. Error bars indicate standard deviation between ChIP experiments with at least three independent ...

Autosomal-linked histone modifications

Our analysis of two X-linked chromosomal regions showed that H3K9me3 is present within active coding regions, whereas H3K27me3 is an abundant mark within intergenic, promoter and coding regions of inactive genes. To determine whether such patterns are X specific, we analysed several unexpressed or active autosomal loci in XY lymphoblastoid cells. Within unexpressed genes (myoglobin and BDNF (brain-derived neuronal factor)), H3K27me3 was predominant throughout intergenic, coding and promoter regions (Fig 4A). Coding regions of active genes (GAPDH and β-actin) contained H3K9me3 and lacked H3K27me3 (Fig 4B). At these active loci, intergenic regions were devoid of H3K27me3. Thus, apart from a less pronounced H3K27me3 within intergenic regions that flank active loci, essentially similar patterns of modification were observed. Active histone marks colocalized at promoter regions, but seemed to be less localized than X-linked genes.


Our analysis shows that intergenic, coding and promoter regions of two regions of the X chromosome are segregated into differentially marked chromatin: H3K27me3 marks intergenic regions, silent genes, but also some active genes; H3K9me3 is predominant in coding regions of active genes; that is, chromatin that is not generally regarded as heterochromatic. None of these features is specific for (escape from) X inactivation, although widespread intergenic H3K27me3 may be more prominent on the X chromosome. Possibly, X-inactivation-specific features, if present, could involve the association of XIST, MacroH2A or histone modifications not analysed in this study.

The presence of H3K27me3 at unexpressed autosomal genes suggests that this mark may be a more global (facultative) heterochromatic mark rather than an Xi-specific mark as anticipated. The reported interphase Xi-specific immunostaining of H3K27me3 (Plath et al, 2003; Silva et al, 2003; Kohlmaier et al, 2004) could be attributed to the higher density of inactive genes and/or the highly condensed structure of the Xi. Chromatin of the Xi has been reported to be enriched for H3K9me2 (Peters et al, 2003), but within the genes analysed here, the overall levels are low. H3K9me2 has been detected in a ‘hotspot' upstream of mouse XIST (Heard et al, 2001; Rougeulle et al, 2004), and it is possible that local enrichment of H3K9me2 in such hotspots is characteristic for this mark and was therefore not detected in our analysis.

Although H3K9 methylation and binding of HP1 to H3K9 methylated histones have been widely correlated with heterochromatin formation, it was reported recently that H3K9 methylation and HP1γ are enriched in transcribed regions of active mammalian genes (Vakoc et al, 2005). The data presented here support the idea that this may be a general phenomenon and indicate that these marks are not exclusive for heterochromatin.

Transitions between H3K27me3 and H3K9me3 occur at positions containing local enrichment of H3K4me3 and H3/H4ac, suggesting a possible role for these active marks in the partitioning of differentially marked chromatin. Colocalization of H3K4 methylation and H3/H4 acetylation at promoter regions of genes escaping X inactivation has been reported previously (Boggs et al, 2002; Goto et al, 2002) but seems to be a general phenomenon rather than X specific, as it has been described for yeast (Ng et al, 2003; Roh et al, 2004; Pokholok et al, 2005), Drosophila (Schubeler et al, 2004), chicken (Schneider et al, 2004), human and mouse (Liang et al, 2004; Bernstein et al, 2005). The presence of such colocalized modifications around transcription start sites positively correlates with gene activity, but strictly taken, such active code is not exclusive for actively transcribed genes, as similar levels of these modifications also colocalize at the silent CITED1 and PHKA1 genes in lymphoblastoid cells. The maintenance of the active code at these genes may be related to their facultative expression patterns.

Microscopic analysis suggested that human Xi chromatin is segregated into non-overlapping types of heterochromatin, dominated by either H3K9me3 or H3K27me3 (Chadwick & Willard, 2004). Most of the human Xi was stained by anti-H3K9me3, whereas two chromosomal bands (Xp11 and Xq23) were stained by anti-H3K27me3. The chromosomal regions analysed in our study (Xp22.22 and Xq13.1) correspond to H3K9me3-staining chromatin; nevertheless, we find abundant H3K27me3. Because of structural determinants such as chromosome condensation, immunofluorescence microscopic analysis may not be directly comparable with gene-by-gene ChIP analysis.

Although it is clear that differentially marked chromatin can be distinguished at intergenic, coding and promoter regions, it is unknown how these different types of chromatin are established, and whether this differential marking is the cause or the consequence of continuing transcription or subnuclear localization. The results shown here disagree with the concept that heterochromatin and euchromatin can be defined on the basis of individual histone modifications. Instead, composite patterns consisting of interdependent or mutually exclusive histone modifications signal the transcriptional status of chromatin.


Cell culture. Hamster–human hybrid cell lines 578 (Xa, Wg3H hamster background) and 789 (Xi, hamster A3 background) have been described (Wieacker et al, 1984), and were cultured in DMEM medium and 10% FCS. Human lymphoblastoid cells were cultured in RPMI medium and 10% FCS.

Antibodies. The following antibodies were used for ChIPs: H3ac, Upstate (Lake Placid, NY, USA) #06-599; H4ac, Upstate #06-866; H3K4me3, Abcam (Cambridge, UK); H3K9me2, Upstate #07-212; H3K9me3 #4861 (Peters et al, 2003); H3K27me3 #6523 (Peters et al, 2003). Specific details on these antibodies are described in the supplementary information online.

Chromatin immunoprecipitations. A detailed protocol for ChIPs and reChIPs is described in supplementary information online.

Quantitative real-time PCR. Quantitative real-time PCR was performed using iQ SYBR green Supermix and a single-colour detection MyIQ iCycler (Bio-Rad, Hercules, CA, USA). Primers used for quantitative real-time PCR are listed in supplementary Table S2 online. To show that these primers selectively amplify human sequences from hamster–human hybrid cells, amplicon melting curves are provided for amplification of hamster–human hybrid DNA, human lymphoblastoid DNA and parental hamster DNA lacking the human X chromosome.

Supplementary information is available at EMBO reports online (http://www.emboreports.org).

Supplementary Material

Supplementary Material


We thank H. van Bokhoven and H. Brunner for helpful discussions and providing cell lines. A.B.B. is supported by the Dutch Cancer Foundation (KWF) grant KUN 2003-2932. J.H.A.M. is supported by an EMBO long-term fellowship, and research in the laboratory of T.J. is sponsored by the IMP through Boehringer Ingelheim and by grants from the European Union (NoE network ‘The Epigenome' LSHG-CT-2004-503433) and the Austrian GEN-AU initiative, which is financed by funds from the Austrian Federal Ministry for Education, Science and Culture (BMBWK).


  • Bernstein BE et al. (2005) Genomic maps and comparative analysis of histone modifications in human and mouse. Cell 120: 169–181 [PubMed]
  • Boggs BA, Cheung P, Heard E, Spector DL, Chinault AC, Allis CD (2002) Differentially methylated forms of histone H3 show unique association patterns with inactive human X chromosomes. Nat Genet 30: 73–76 [PubMed]
  • Brown CJ, Ballabio A, Rupert JL, Lafreniere RG, Grompe M, Tonlorenzi R, Willard HF (1991) A gene from the region of the human X inactivation centre is expressed exclusively from the inactive X chromosome. Nature 349: 38–44 [PubMed]
  • Cao R, Wang L, Wang H, Xia L, Erdjument-Bromage H, Tempst P, Jones RS, Zhang Y (2002) Role of histone H3 lysine 27 methylation in Polycomb-group silencing. Science 298: 1039–1043 [PubMed]
  • Carrel L, Willard HF (2005) X-inactivation profile reveals extensive variability in X-linked gene expression in females. Nature 434: 400–404 [PubMed]
  • Chadwick BP, Willard HF (2004) Multiple spatially distinct types of facultative heterochromatin on the human inactive X chromosome. Proc Natl Acad Sci USA 101: 17450–17455 [PMC free article] [PubMed]
  • Costanzi C, Pehrson JR (1998) Histone macroH2A1 is concentrated in the inactive X chromosome of female mammals. Nature 393: 599–601 [PubMed]
  • Goto Y, Gomez M, Brockdorff N, Feil R (2002) Differential patterns of histone methylation and acetylation distinguish active and repressed alleles at X-linked genes. Cytogenet Genome Res 99: 66–74 [PubMed]
  • Heard E, Rougeulle C, Arnaud D, Avner P, Allis CD, Spector DL (2001) Methylation of histone H3 at Lys-9 is an early mark on the X chromosome during X inactivation. Cell 107: 727–738 [PubMed]
  • Jeppesen P, Turner BM (1993) The inactive X chromosome in female mammals is distinguished by a lack of histone H4 acetylation, a cytogenetic marker for gene expression. Cell 74: 281–289 [PubMed]
  • Kirmizis A, Bartley SM, Kuzmichev A, Margueron R, Reinberg D, Green R, Farnham PJ (2004) Silencing of human polycomb target genes is associated with methylation of histone H3 Lys 27. Genes Dev 18: 1592–1605 [PMC free article] [PubMed]
  • Kohlmaier A, Savarese F, Lachner M, Martens J, Jenuwein T, Wutz A (2004) A chromosomal memory triggered by XIST regulates histone methylation in X inactivation. PLoS Biol 2: E171. [PMC free article] [PubMed]
  • Liang G et al. (2004) Distinct localization of histone H3 acetylation and H3-K4 methylation to the transcription start sites in the human genome. Proc Natl Acad Sci USA 101: 7357–7362 [PMC free article] [PubMed]
  • Ng HH, Robert F, Young RA, Struhl K (2003) Targeted recruitment of Set1 histone methylase by elongating Pol II provides a localized mark and memory of recent transcriptional activity. Mol Cell 11: 709–719 [PubMed]
  • Norris DP, Brockdorff N, Rastan S (1991) Methylation status of CpG-rich islands on active and inactive mouse X chromosomes. Mamm Genome 1: 78–83 [PubMed]
  • Peters AH et al. (2003) Partitioning and plasticity of repressive histone methylation states in mammalian chromatin. Mol Cell 12: 1577–1589 [PubMed]
  • Plath K, Fang J, Mlynarczyk-Evans SK, Cao R, Worringer KA, Wang H, de la Cruz CC, Otte AP, Panning B, Zhang Y (2003) Role of histone H3 lysine 27 methylation in X inactivation. Science 300: 131–135 [PubMed]
  • Pokholok DK et al. (2005) Genome-wide map of nucleosome acetylation and methylation in yeast. Cell 122: 517–527 [PubMed]
  • Rocchigiani M, Lestingi M, Luddi A, Orlandini M, Franco B, Rossi E, Ballabio A, Zuffardi O, Oliviero S (1998) Human FIGF: cloning, gene structure, and mapping to chromosome Xp22.1 between the PIGA and the GRPR genes. Genomics 47: 207–216 [PubMed]
  • Roh TY, Ngau WC, Cui K, Landsman D, Zhao K (2004) High-resolution genome-wide mapping of histone modifications. Nat Biotechnol 22: 1013–1016 [PubMed]
  • Rougeulle C, Chaumeil J, Sarma K, Allis CD, Reinberg D, Avner P, Heard E (2004) Differential histone H3 Lys-9 and Lys-27 methylation profiles on the X chromosome. Mol Cell Biol 24: 5475–5484 [PMC free article] [PubMed]
  • Schneider R, Bannister AJ, Myers FA, Thorne AW, Crane-Robinson C, Kouzarides T (2004) Histone H3 lysine 4 methylation patterns in higher eukaryotic genes. Nat Cell Biol 6: 73–77 [PubMed]
  • Schotta G, Lachner M, Sarma K, Ebert A, Sengupta R, Reuter G, Reinberg D, Jenuwein T (2004) A silencing pathway to induce H3-K9 and H4-K20 trimethylation at constitutive heterochromatin. Genes Dev 18: 1251–1262 [PMC free article] [PubMed]
  • Schubeler D et al. (2004) The histone modification pattern of active genes revealed through genome-wide chromatin analysis of a higher eukaryote. Genes Dev 18: 1263–1271 [PMC free article] [PubMed]
  • Silva J, Mak W, Zvetkova I, Appanah R, Nesterova TB, Webster Z, Peters AH, Jenuwein T, Otte AP, Brockdorff N (2003) Establishment of histone h3 methylation on the inactive X chromosome requires transient recruitment of Eed–Enx1 polycomb group complexes. Dev Cell 4: 481–495 [PubMed]
  • Vakoc CR, Mandat SA, Olenchock BA, Blobel GA (2005) Histone H3 lysine 9 methylation and HP1gamma are associated with transcription elongation through mammalian chromatin. Mol Cell 19: 381–391 [PubMed]
  • Wendler WM, Kremmer E, Forster R, Winnacker EL (1997) Identification of pirin, a novel highly conserved nuclear protein. J Biol Chem 272: 8482–8489 [PubMed]
  • Wieacker P, Davies KE, Cooke HJ, Pearson PL, Williamson R, Bhattacharya S, Zimmer J, Ropers HH (1984) Toward a complete linkage map of the human X chromosome: regional assignment of 16 cloned single-copy DNA sequences employing a panel of somatic cell hybrids. Am J Hum Genet 36: 265–276 [PMC free article] [PubMed]

Articles from EMBO Reports are provided here courtesy of The European Molecular Biology Organization
PubReader format: click here to try


Save items

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


  • Cited in Books
    Cited in Books
    NCBI Bookshelf books that cite the current articles.
  • MedGen
    Related information in MedGen
  • PubMed
    PubMed citations for these articles
  • Substance
    PubChem chemical substance records that cite the current articles. These references are taken from those provided on submitted PubChem chemical substance records.
  • Taxonomy
    Taxonomy records associated with the current articles through taxonomic information on related molecular database records (Nucleotide, Protein, Gene, SNP, Structure).
  • Taxonomy Tree
    Taxonomy Tree

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...