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Nucleic Acids Res. Jun 1, 1998; 26(11): 2829–2830.
PMCID: PMC147619

An alternative PCR assay for quantifying mitochondrial DNA in crude preparations.

Abstract

A method is described for the quantification of mitochondrial DNA present in crude biological preparations. A known copy number of a standard is amplified in the presence of inactivated target DNA so as to determine the overall efficiency of the PCR process in a particular sample. In this way any inhibitory and/or stimulatory substances present in sample preparations can be taken into account. To reduce tube-to-tube variations product DNA quantification is limited to small cycle numbers. Using this method quantitations of DNA amounts in different crude preparations can be compared.

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Selected References

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  • Gilliland G, Perrin S, Blanchard K, Bunn HF. Analysis of cytokine mRNA and DNA: detection and quantitation by competitive polymerase chain reaction. Proc Natl Acad Sci U S A. 1990 Apr;87(7):2725–2729. [PMC free article] [PubMed]
  • Raeymaekers L. A commentary on the practical applications of competitive PCR. Genome Res. 1995 Aug;5(1):91–94. [PubMed]
  • Ahn SJ, Costa J, Emanuel JR. PicoGreen quantitation of DNA: effective evaluation of samples pre- or post-PCR. Nucleic Acids Res. 1996 Jul 1;24(13):2623–2625. [PMC free article] [PubMed]

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