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Genetics. 2004 August; 167(4): 1791–1799. doi: 10.1534/genetics.104.026583. | PMCID: PMC1471026 |
Extensive sex-specific nonadditivity of gene expression in Drosophila melanogaster. Greg Gibson, Rebecca Riley-Berger, Larry Harshman, Artyom Kopp, Scott Vacha, Sergey Nuzhdin, and Marta Wayne Department of Genetics, North Carolina State University, Raleigh, North Carolina 27695, USA. Assessment of the degree to which gene expression is additive and heritable has important implications for understanding the maintenance of variation, adaptation, phenotypic divergence, and the mapping of genotype onto phenotype. We used whole-genome transcript profiling using Agilent long-oligonucleotide microarrays representing 12,017 genes to demonstrate that gene transcription is pervasively nonadditive in Drosophila melanogaster. Comparison of adults of two isogenic lines and their reciprocal F1 hybrids revealed 5820 genes as significantly different between at least two of the four genotypes in either males or females or across both sexes. Strikingly, while 25% of all genes differ between the two parents, 33% differ between both F1's and the parents, averaged across sexes. However, only 5% of genes show overdominance, suggesting that heterosis for expression is rare. The Full Text of this article is available as a PDF (181K). These references are in PubMed. This may not be the complete list of references from this article. - Arbeitman Michelle N, Furlong Eileen E M, Imam Farhad, Johnson Eric, Null Brian H, Baker Bruce S, Krasnow Mark A, Scott Matthew P, Davis Ronald W, White Kevin P. Gene expression during the life cycle of Drosophila melanogaster. Science. 2002 Sep 27;297(5590):2270–2275. [PubMed]
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