• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of geneticsGeneticsCurrent IssueInformation for AuthorsEditorial BoardSubscribeSubmit a Manuscript
Genetics. May 2004; 167(1): 203–206.
PMCID: PMC1470872

The Zuker collection: a resource for the analysis of autosomal gene function in Drosophila melanogaster.

Abstract

The majority of genes of multicellular organisms encode proteins with functions that are not required for viability but contribute to important physiological functions such as behavior and reproduction. It is estimated that 75% of the genes of Drosophila melanogaster are nonessential. Here we report on a strategy used to establish a large collection of stocks that is suitable for the recovery of mutations in such genes. From approximately 72,000 F(3) cultures segregating for autosomes heavily treated with ethyl methanesulfonate (EMS), approximately 12,000 lines in which the treated second or third chromosome survived in homozygous condition were selected. The dose of EMS induced an estimated rate of 1.2-1.5 x 10(-3) mutations/gene and predicts five to six nonessential gene mutations per chromosome and seven to nine alleles per locus in the samples of 6000 second chromosomes and 6000 third chromosomes. Due to mosaic mutations induced in the initial exposure to the mutagen, many of the lines are segregating or are now fixed for lethal mutations on the mutagenized chromosome. The features of this collection, known as the Zuker collection, make it a valuable resource for forward and reverse genetic screens for mutations affecting a wide array of biological functions.

Full Text

The Full Text of this article is available as a PDF (62K).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Brizuela BJ, Elfring L, Ballard J, Tamkun JW, Kennison JA. Genetic analysis of the brahma gene of Drosophila melanogaster and polytene chromosome subdivisions 72AB. Genetics. 1994 Jul;137(3):803–813. [PMC free article] [PubMed]
  • Dolph PJ, Ranganathan R, Colley NJ, Hardy RW, Socolich M, Zuker CS. Arrestin function in inactivation of G protein-coupled receptor rhodopsin in vivo. Science. 1993 Jun 25;260(5116):1910–1916. [PubMed]
  • Epler JL. Ethyl methanesulfonate-induced lethals in Drosophila--frequency-dose relations and multiple mosaicism. Genetics. 1966 Jul;54(1):31–36. [PMC free article] [PubMed]
  • Gibbs SM, Becker A, Hardy RW, Truman JW. Soluble guanylate cyclase is required during development for visual system function in Drosophila. J Neurosci. 2001 Oct 1;21(19):7705–7714. [PubMed]
  • Giunta Kelly L, Jang Janet K, Manheim Elizabeth A, Subramanian Gayathri, McKim Kim S. subito encodes a kinesin-like protein required for meiotic spindle pole formation in Drosophila melanogaster. Genetics. 2002 Apr;160(4):1489–1501. [PMC free article] [PubMed]
  • Laurencon Anne, Orme Charisse M, Peters Heather K, Boulton Christina L, Vladar Eszter K, Langley Sasha A, Bakis Emmanuel P, Harris David T, Harris Nathan J, Wayson Sarah M, et al. A large-scale screen for mutagen-sensitive loci in Drosophila. Genetics. 2004 May;167(1):217–231. [PMC free article] [PubMed]
  • Manheim Elizabeth A, McKim Kim S. The Synaptonemal complex component C(2)M regulates meiotic crossing over in Drosophila. Curr Biol. 2003 Feb 18;13(4):276–285. [PubMed]
  • McCallum CM, Comai L, Greene EA, Henikoff S. Targeted screening for induced mutations. Nat Biotechnol. 2000 Apr;18(4):455–457. [PubMed]
  • Miklos GL, Rubin GM. The role of the genome project in determining gene function: insights from model organisms. Cell. 1996 Aug 23;86(4):521–529. [PubMed]
  • Niemeyer BA, Suzuki E, Scott K, Jalink K, Zuker CS. The Drosophila light-activated conductance is composed of the two channels TRP and TRPL. Cell. 1996 May 31;85(5):651–659. [PubMed]
  • Acharya JK, Labarca P, Delgado R, Jalink K, Zuker CS. Synaptic defects and compensatory regulation of inositol metabolism in inositol polyphosphate 1-phosphatase mutants. Neuron. 1998 Jun;20(6):1219–1229. [PubMed]
  • Tsunoda S, Sierralta J, Sun Y, Bodner R, Suzuki E, Becker A, Socolich M, Zuker CS. A multivalent PDZ-domain protein assembles signalling complexes in a G-protein-coupled cascade. Nature. 1997 Jul 17;388(6639):243–249. [PubMed]
  • Bentley A, MacLennan B, Calvo J, Dearolf CR. Targeted recovery of mutations in Drosophila. Genetics. 2000 Nov;156(3):1169–1173. [PMC free article] [PubMed]
  • Wakimoto Barbara T, Lindsley Dan L, Herrera Cheryl. Toward a comprehensive genetic analysis of male fertility in Drosophila melanogaster. Genetics. 2004 May;167(1):207–216. [PMC free article] [PubMed]
  • Zelhof Andrew C, Koundakjian Edmund, Scully Audra L, Hardy Robert W, Pounds Linda. Mutation of the photoreceptor specific homeodomain gene Pph13 results in defects in phototransduction and rhabdomere morphogenesis. Development. 2003 Sep;130(18):4383–4392. [PubMed]

Articles from Genetics are provided here courtesy of Genetics Society of America

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...