Logo of narLink to Publisher's site
Nucleic Acids Res. 1997 Aug 1; 25(15): 3183–3185.
PMCID: PMC146847

Magnetic bead capture of cDNAs from double-stranded plasmid cDNA libraries.


We have developed a cDNA library screening method which allows the simultaneous screening of >10 ( 12 ) double-stranded plasmid cDNA molecules with minimal a priori sequence knowledge. A biotinylated, gene-specific oligonucleotide probe along with abutting 'blocking' oligos is hybridized to the plasmid cDNA library and the target plasmid retrieved with paramagnetic streptavidin beads and transformed into Escherichia coli. Multiple rounds of enrichment with a target plasmid represented at 0.002-0.0001% resulted in over one-third positive clones. Our method will be useful for isolating even the rarest cDNAs starting from ESTs, isolated exons or homologous sequence information.

Full Text

The Full Text of this article is available as a PDF (66K).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Pippin J, Contractor V, Ambrus JL., Jr An inexpensive method for the rapid identification of specific cDNAs from cDNA libraries. Biotechniques. 1994 Apr;16(4):676–680. [PubMed]
  • Tagle DA, Swaroop M, Lovett M, Collins FS. Magnetic bead capture of expressed sequences encoded within large genomic segments. Nature. 1993 Feb 25;361(6414):751–753. [PubMed]
  • Sandhu GS, Precup JW, Kline BC. Rapid one-step characterization of recombinant vectors by direct analysis of transformed Escherichia coli colonies. Biotechniques. 1989 Jul-Aug;7(7):689–690. [PubMed]
  • Hakvoort TB, Spijkers JA, Vermeulen JL, Lamers WH. Preparation of a differentially expressed, full-length cDNA expression library by RecA-mediated triple-strand formation with subtractively enriched cDNA fragments. Nucleic Acids Res. 1996 Sep 1;24(17):3478–3480. [PMC free article] [PubMed]
  • Kato K. Description of the entire mRNA population by a 3' end cDNA fragment generated by class IIS restriction enzymes. Nucleic Acids Res. 1995 Sep 25;23(18):3685–3690. [PMC free article] [PubMed]
  • Ivanova NB, Belyavsky AV. Identification of differentially expressed genes by restriction endonuclease-based gene expression fingerprinting. Nucleic Acids Res. 1995 Aug 11;23(15):2954–2958. [PMC free article] [PubMed]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...