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Br J Clin Pharmacol. Jul 1984; 18(1): 9–15.
PMCID: PMC1463577

Nicotine and cotinine concentrations in serum and milk of nursing smokers.


Analysis of 44 milk samples from 23 nursing smokers revealed that there was a linear correlation between nicotine concentrations in serum and in milk (r = 0.70). The nicotine concentrations in milk were considerably higher than the corresponding serum concentrations: milk/serum concentration ratio = 2.92 +/- 1.09; (n = 44). There was also a linear correlation between the cotinine concentrations in serum and in milk (r = 0.89). The cotinine concentrations in milk were lower than the corresponding serum concentrations: milk/serum concentration ratio = 0.78 +/- 0.19; (n = 44). The direct comparison between the half-lives of nicotine in serum and in milk was possible in five nursing smokers. The half-life of nicotine in milk was determined in four additional smoking mothers. The half-life of nicotine in milk t 1/2 = 97 +/- 20 min slightly exceeded the half-life of nicotine in serum t 1/2 = 81 +/- 9 min; the difference between these two values was not statistically significant (P greater than 0.05). Cotinine concentrations remained fairly consistent during a 4 h interval without smoking.

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Selected References

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  • Ansell C, Moore A, Barrie H. Electrolyte pH changes in Human Milk. Pediatr Res. 1977 Dec;11(12):1177–1179. [PubMed]
  • Armitage AK, Dollery CT, George CF, Houseman TH, Lewis PJ, Turner DM. Absorption and metabolism of nicotine from cigarettes. Br Med J. 1975 Nov 8;4(5992):313–316. [PMC free article] [PubMed]
  • Beckett AH, Gorrod JW, Jenner P. A possible relation between pKa 1 and lipid solubility and the amounts excreted in urine of some tobacco alkaloids given to man. J Pharm Pharmacol. 1972 Feb;24(2):115–120. [PubMed]
  • Benowitz NL, Hall SM, Herning RI, Jacob P, 3rd, Jones RT, Osman AL. Smokers of low-yield cigarettes do not consume less nicotine. N Engl J Med. 1983 Jul 21;309(3):139–142. [PubMed]
  • Benowitz NL, Jacob P, 3rd, Jones RT, Rosenberg J. Interindividual variability in the metabolism and cardiovascular effects of nicotine in man. J Pharmacol Exp Ther. 1982 May;221(2):368–372. [PubMed]
  • Ferguson BB, Wilson DJ, Schaffner W. Determination of nicotine concentrations in human milk. Am J Dis Child. 1976 Aug;130(8):837–839. [PubMed]
  • Feyerabend C, Russell MA. Improved gas chromatographic method and micro-extraction technique for the measurement of nicotine in biological fluids. J Pharm Pharmacol. 1979 Feb;31(2):73–76. [PubMed]
  • Feyerabend C, Russell MA. Rapid gas-liquid chromatographic determination of cotinine in biological fluids. Analyst. 1980 Oct;105(1255):998–1001. [PubMed]
  • Hall B. Changing composition of human milk and early development of an appetite control. Lancet. 1975 Apr 5;1(7910):779–781. [PubMed]
  • Hardee GE, Stewart T, Capomacchia AC. Tobacco smoke xenobiotic compound appearance in mothers' milk after involuntary smoke exposures. I. Nicotine and cotinine. Toxicol Lett. 1983 Feb;15(2-3):109–112. [PubMed]
  • Hengen N, Hengen M. Gas-liquid chromatographic determination of nicotine and cotinine in plasma. Clin Chem. 1978 Jan;24(1):50–53. [PubMed]
  • Hill P, Haley NJ, Wynder EL. Cigarette smoking: carboxyhemoglobin, plasma nicotine, cotinine and thiocyanate vs self-reported smoking data and cardiovascular disease. J Chronic Dis. 1983;36(6):439–449. [PubMed]
  • Isaac PF, Rand MJ. Cigarette smoking and plasma levels of nicotine. Nature. 1972 Apr 7;236(5345):308–310. [PubMed]
  • Kuzma JW, Kissinger DG. Patterns of alcohol and cigarette use in pregnancy. Neurobehav Toxicol Teratol. 1981 Summer;3(2):211–221. [PubMed]
  • Kyerematen GA, Damiano MD, Dvorchik BH, Vesell ES. Smoking-induced changes in nicotine disposition: application of a new HPLC assay for nicotine and its metabolites. Clin Pharmacol Ther. 1982 Dec;32(6):769–780. [PubMed]
  • Kyerematen GA, Dvorchik BH, Vesell ES. Influence of different forms of tobacco intake on nicotine elimination in man. Pharmacology. 1983;26(4):205–209. [PubMed]
  • Langone JJ, Van Vunakis H. Quantitation of continine in sera of smokers. Res Commun Chem Pathol Pharmacol. 1975 Jan;10(1):21–28. [PubMed]
  • Oldendorf WH. Lipid solubility and drug penetration of the blood brain barrier. Proc Soc Exp Biol Med. 1974 Dec;147(3):813–815. [PubMed]
  • Perlman HH, Dannenberg AM, Sokoloff N. The excretion of nicotine in breast milk and urine from cigarette smoking: its effect on lactation and the nursing. J Am Med Assoc. 1942 Nov 28;120(13):1003–1009. [PubMed]
  • Rickert WS, Robinson JC. Estimating the hazards of less hazardous cigarettes. II. Study of cigarette yields of nicotine, carbon monoxide, and hydrogen cyanide in relation to levels of cotinine, carboxyhemoglobin, and thiocyanate in smokers. J Toxicol Environ Health. 1981 Mar-Apr;7(3-4):391–403. [PubMed]
  • Rosenberg J, Benowitz NL, Jacob P, Wilson KM. Disposition kinetics and effects of intravenous nicotine. Clin Pharmacol Ther. 1980 Oct;28(4):517–522. [PubMed]
  • Russell MA, Feyerabend C. Cigarette smoking: a dependence on high-nicotine boli. Drug Metab Rev. 1978;8(1):29–57. [PubMed]
  • Russell MA, Jarvis M, Iyer R, Feyerabend C. Relation of nicotine yield of cigarettes to blood nicotine concentrations in smokers. Br Med J. 1980 Apr 5;280(6219):972–976. [PMC free article] [PubMed]
  • Russell MA, Wilson C, Patel UA, Feyerabend C, Cole PV. Plasma nicotine levels after smoking cigarettes with high, medium, and low nicotine yields. Br Med J. 1975 May 24;2(5968):414–416. [PMC free article] [PubMed]
  • Schmeltz I, Hoffmann D, Wynder EL. The influence of tobacco smoke on indoor atmospheres. Prev Med. 1975 Mar;4(1):66–82. [PubMed]
  • Yamamoto I. Nicotinoids as insecticides. Adv Pest Control Res. 1965;6:231–260. [PubMed]

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