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Genetics. Nov 2003; 165(3): 1533–1540.
PMCID: PMC1462840

Partial diploidization of meiosis in autotetraploid Arabidopsis thaliana.

Abstract

Meiosis was analyzed cytogenetically in autotetraploids of Arabidopsis, including both established lines and newly generated autotetraploid plants. Fluorescent in situ hybridization with 5S and 45S rDNA probes was used to identify the different chromosomes at metaphase I of meiosis. Multivalents were observed frequently in all the lines analyzed, but there were significant differences in multivalent frequency not only between the newly generated tetraploids and the established lines but also among the different established lines. The new tetraploids showed high multivalent frequencies, exceeding the theoretical 66.66% predicted by the simple random-end pairing model, in some cases significantly, thus indicating that Arabidopsis autotetraploids have more than two autonomous pairing sites per chromosome, despite their small sizes. The established lines showed fewer multivalents than the new autotetraploids did, but the extent of this reduction was strongly line and chromosome dependent. One line in particular showed a large reduction in multivalents and a concomitant increase in bivalents, while the other lines showed lesser reductions in multivalents. The reduction in multivalents was not uniformly distributed across chromosomes. The smaller chromosomes, especially chromosomes 2 and 4, showed the most marked reductions while the largest chromosome (1) showed virtually no reduction compared to the new tetraploids. It is concluded that the established autotetraploid lines have undergone a partial diploidization of meiosis, but not necessarily genetical diploidization, since their creation. Possible mechanisms for the resulting change in meiotic chromosome behavior are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Callow RS, Gladwell I. A general treatment of chromosome synapsis in even-numbered polyploids. J Theor Biol. 1984 Feb 21;106(4):455–494. [PubMed]
  • Díez M, Santos JL, Cuñado N, Naranjo T. Meiosis in primary trisomics of rye: considerations for models of chromosome pairing. Chromosome Res. 2001;9(1):13–23. [PubMed]
  • Mitchell-Olds Thomas, Clauss Maria J. Plant evolutionary genomics. Curr Opin Plant Biol. 2002 Feb;5(1):74–79. [PubMed]
  • Fransz P, Armstrong S, Alonso-Blanco C, Fischer TC, Torres-Ruiz RA, Jones G. Cytogenetics for the model system Arabidopsis thaliana. Plant J. 1998 Mar;13(6):867–876. [PubMed]
  • Sanchez Moran E, Armstrong SJ, Santos JL, Franklin FC, Jones GH. Chiasma formation in Arabidopsis thaliana accession Wassileskija and in two meiotic mutants. Chromosome Res. 2001;9(2):121–128. [PubMed]
  • Sanchez-Moran E, Armstrong SJ, Santos JL, Franklin FCH, Jones GH. Variation in chiasma frequency among eight accessions of Arabidopsis thaliana. Genetics. 2002 Nov;162(3):1415–1422. [PMC free article] [PubMed]
  • Santos JL, Orellana J, Giraldez R. Pairing Competition between Identical and Homologous Chromosomes in Rye and Grasshoppers. Genetics. 1983 Aug;104(4):677–684. [PMC free article] [PubMed]
  • Santos JL, Cuadrado MC, Díez M, Romero C, Cuñado N, Naranjo T, Martínez M. Further insights on chromosomal pairing of autopolyploids: a triploid and tetraploids of rye. Chromosoma. 1995 Dec;104(4):298–307. [PubMed]
  • Jones GH, Vincent JE. Meiosis in allopolyploid Crepis capillaris. II. Autotetraploids. Genome. 1994 Jun;37(3):497–505. [PubMed]
  • Weiss H, Maluszynska J. Chromosomal rearrangement in autotetraploid plants of Arabidopsis thaliana. Hereditas. 2000;133(3):255–261. [PubMed]
  • Koornneef Maarten, Fransz Paul, de Jong Hans. Cytogenetic tools for Arabidopsis thaliana. Chromosome Res. 2003;11(3):183–194. [PubMed]

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