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Genetics. Sep 2003; 165(1): 277–285.
PMCID: PMC1462731

Haplotype sharing refines the location of an imprinted quantitative trait locus with major effect on muscle mass to a 250-kb chromosome segment containing the porcine IGF2 gene.


We herein describe the fine mapping of an imprinted QTL with major effect on muscle mass that was previously assigned to distal SSC2p in the pig. The proposed approach exploits linkage disequilibrium in combination with QTL genotyping by marker-assisted segregation analysis. By identifying a haplotype shared by all "Q" chromosomes, we map the QTL to an approximately 250-kb chromosome segment containing INS and IGF2 as the only known paternally expressed genes. This considerably reinforces the candidacy of these genes, justifying their detailed analysis.

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  • Georges M, Nielsen D, Mackinnon M, Mishra A, Okimoto R, Pasquino AT, Sargeant LS, Sorensen A, Steele MR, Zhao X, et al. Mapping quantitative trait loci controlling milk production in dairy cattle by exploiting progeny testing. Genetics. 1995 Feb;139(2):907–920. [PMC free article] [PubMed]
  • Grisart Bernard, Coppieters Wouter, Farnir Frédéric, Karim Latifa, Ford Christine, Berzi Paulette, Cambisano Nadine, Mni Myriam, Reid Suzanne, Simon Patricia, et al. Positional candidate cloning of a QTL in dairy cattle: identification of a missense mutation in the bovine DGAT1 gene with major effect on milk yield and composition. Genome Res. 2002 Feb;12(2):222–231. [PubMed]
  • Grobet L, Martin LJ, Poncelet D, Pirottin D, Brouwers B, Riquet J, Schoeberlein A, Dunner S, Ménissier F, Massabanda J, et al. A deletion in the bovine myostatin gene causes the double-muscled phenotype in cattle. Nat Genet. 1997 Sep;17(1):71–74. [PubMed]
  • Jeon JT, Carlborg O, Törnsten A, Giuffra E, Amarger V, Chardon P, Andersson-Eklund L, Andersson K, Hansson I, Lundström K, et al. A paternally expressed QTL affecting skeletal and cardiac muscle mass in pigs maps to the IGF2 locus. Nat Genet. 1999 Feb;21(2):157–158. [PubMed]
  • Mackay TF. Quantitative trait loci in Drosophila. Nat Rev Genet. 2001 Jan;2(1):11–20. [PubMed]
  • Mauricio R. Mapping quantitative trait loci in plants: uses and caveats for evolutionary biology. Nat Rev Genet. 2001 May;2(5):370–381. [PubMed]
  • Meuwissen TH, Goddard ME. Prediction of identity by descent probabilities from marker-haplotypes. Genet Sel Evol. 2001 Nov-Dec;33(6):605–634. [PMC free article] [PubMed]
  • Milan D, Jeon JT, Looft C, Amarger V, Robic A, Thelander M, Rogel-Gaillard C, Paul S, Iannuccelli N, Rask L, et al. A mutation in PRKAG3 associated with excess glycogen content in pig skeletal muscle. Science. 2000 May 19;288(5469):1248–1251. [PubMed]
  • Amarger Valérie, Nguyen Minh, Van Laere Anne-Sophie, Braunschweig Martin, Nezer Carine, Georges Michel, Andersson Leif. Comparative sequence analysis of the INS-IGF2-H19 gene cluster in pigs. Mamm Genome. 2002 Jul;13(7):388–398. [PubMed]
  • Nezer C, Moreau L, Brouwers B, Coppieters W, Detilleux J, Hanset R, Karim L, Kvasz A, Leroy P, Georges M. An imprinted QTL with major effect on muscle mass and fat deposition maps to the IGF2 locus in pigs. Nat Genet. 1999 Feb;21(2):155–156. [PubMed]
  • Ardlie Kristin G, Kruglyak Leonid, Seielstad Mark. Patterns of linkage disequilibrium in the human genome. Nat Rev Genet. 2002 Apr;3(4):299–309. [PubMed]
  • Nezer Carine, Moreau Laurence, Wagenaar Danny, Georges Michel. Results of a whole genome scan targeting QTL for growth and carcass traits in a Piétrain x Large White intercross. Genet Sel Evol. 2002 May-Jun;34(3):371–387. [PMC free article] [PubMed]
  • Blott Sarah, Kim Jong-Joo, Moisio Sirja, Schmidt-Küntzel Anne, Cornet Anne, Berzi Paulette, Cambisano Nadine, Ford Christine, Grisart Bernard, Johnson Dave, et al. Molecular dissection of a quantitative trait locus: a phenylalanine-to-tyrosine substitution in the transmembrane domain of the bovine growth hormone receptor is associated with a major effect on milk yield and composition. Genetics. 2003 Jan;163(1):253–266. [PMC free article] [PubMed]
  • Onyango P, Miller W, Lehoczky J, Leung CT, Birren B, Wheelan S, Dewar K, Feinberg AP. Sequence and comparative analysis of the mouse 1-megabase region orthologous to the human 11p15 imprinted domain. Genome Res. 2000 Nov;10(11):1697–1710. [PubMed]
  • Reik W, Walter J. Genomic imprinting: parental influence on the genome. Nat Rev Genet. 2001 Jan;2(1):21–32. [PubMed]
  • Darvasi A. Experimental strategies for the genetic dissection of complex traits in animal models. Nat Genet. 1998 Jan;18(1):19–24. [PubMed]
  • Riquet J, Coppieters W, Cambisano N, Arranz JJ, Berzi P, Davis SK, Grisart B, Farnir F, Karim L, Mni M, et al. Fine-mapping of quantitative trait loci by identity by descent in outbred populations: application to milk production in dairy cattle. Proc Natl Acad Sci U S A. 1999 Aug 3;96(16):9252–9257. [PMC free article] [PubMed]
  • Dunner S, Charlier C, Farnir F, Brouwers B, Canon J, Georges M. Towards interbreed IBD fine mapping of the mh locus: double-muscling in the Asturiana de los Valles breed involves the same locus as in the Belgian Blue cattle breed. Mamm Genome. 1997 Jun;8(6):430–435. [PubMed]
  • Sabeti Pardis C, Reich David E, Higgins John M, Levine Haninah Z P, Richter Daniel J, Schaffner Stephen F, Gabriel Stacey B, Platko Jill V, Patterson Nick J, McDonald Gavin J, et al. Detecting recent positive selection in the human genome from haplotype structure. Nature. 2002 Oct 24;419(6909):832–837. [PubMed]
  • Fahrenkrug SC, Rohrer GA, Freking BA, Smith TP, Osoegawa K, Shu CL, Catanese JJ, de Jong PJ. A porcine BAC library with tenfold genome coverage: a resource for physical and genetic map integration. Mamm Genome. 2001 Jun;12(6):472–474. [PubMed]
  • Smit Maria, Segers Karin, Carrascosa Laura Garcia, Shay Tracy, Baraldi Francesca, Gyapay Gabor, Snowder Gary, Georges Michel, Cockett Noelle, Charlier Carole. Mosaicism of Solid Gold supports the causality of a noncoding A-to-G transition in the determinism of the callipyge phenotype. Genetics. 2003 Jan;163(1):453–456. [PMC free article] [PubMed]
  • Flint J, Mott R. Finding the molecular basis of quantitative traits: successes and pitfalls. Nat Rev Genet. 2001 Jun;2(6):437–445. [PubMed]
  • Terwilliger JD. A powerful likelihood method for the analysis of linkage disequilibrium between trait loci and one or more polymorphic marker loci. Am J Hum Genet. 1995 Mar;56(3):777–787. [PMC free article] [PubMed]
  • Florini JR, Ewton DZ, Coolican SA. Growth hormone and the insulin-like growth factor system in myogenesis. Endocr Rev. 1996 Oct;17(5):481–517. [PubMed]

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