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Genetics. Jan 2003; 163(1): 295–309.
PMCID: PMC1462420

Cloning of the pleiotropic T locus in soybean and two recessive alleles that differentially affect structure and expression of the encoded flavonoid 3' hydroxylase.


Three loci (I, R, and T) control pigmentation of the seed coats in Glycine max and are genetically distinct from those controlling flower color. The T locus also controls color of the trichome hairs. We report the identification and isolation of a flavonoid 3' hydroxylase gene from G. max (GmF3'H) and the linkage of this gene to the T locus. This GmF3'H gene was highly expressed in early stages of seed coat development and was expressed at very low levels or not at all in other tissues. Evidence that the GmF3'H gene is linked to the T locus came from the occurrence of multiple RFLPs in lines with varying alleles of the T locus, as well as in a population of plants segregating at that locus. GmF3'H genomic and cDNA sequence analysis of color mutant lines with varying t alleles revealed a frameshift mutation in one of the alleles. In another line derived from a mutable genetic stock, the abundance of the mRNAs for GmF3'H was dramatically reduced. Isolation of the GmF3'H gene and its identification as the T locus will enable investigation of the pleiotropic effects of the T locus on cell wall integrity and its involvement in the regulation of the multiple branches of the flavonoid pathway in soybean.

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Selected References

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  • Brugliera F, Barri-Rewell G, Holton TA, Mason JG. Isolation and characterization of a flavonoid 3'-hydroxylase cDNA clone corresponding to the Ht1 locus of Petunia hybrida. Plant J. 1999 Aug;19(4):441–451. [PubMed]
  • Chapple Clint. MOLECULAR-GENETIC ANALYSIS OF PLANT CYTOCHROME P450-DEPENDENT MONOOXYGENASES. Annu Rev Plant Physiol Plant Mol Biol. 1998 Jun;49(NaN):311–343. [PubMed]
  • Feinberg AP, Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. [PubMed]
  • Holton TA, Brugliera F, Lester DR, Tanaka Y, Hyland CD, Menting JG, Lu CY, Farcy E, Stevenson TW, Cornish EC. Cloning and expression of cytochrome P450 genes controlling flower colour. Nature. 1993 Nov 18;366(6452):276–279. [PubMed]
  • Nicholas CD, Lindstrom JT, Vodkin LO. Variation of proline rich cell wall proteins in soybean lines with anthocyanin mutations. Plant Mol Biol. 1993 Jan;21(1):145–156. [PubMed]
  • Todd JJ, Vodkin LO. Pigmented Soybean (Glycine max) Seed Coats Accumulate Proanthocyanidins during Development. Plant Physiol. 1993 Jun;102(2):663–670. [PMC free article] [PubMed]
  • Todd JJ, Vodkin LO. Duplications That Suppress and Deletions That Restore Expression from a Chalcone Synthase Multigene Family. Plant Cell. 1996 Apr;8(4):687–699. [PMC free article] [PubMed]
  • Schoenbohm C, Martens S, Eder C, Forkmann G, Weisshaar B. Identification of the Arabidopsis thaliana flavonoid 3'-hydroxylase gene and functional expression of the encoded P450 enzyme. Biol Chem. 2000 Aug;381(8):749–753. [PubMed]
  • Wang CS, Todd JJ, Vodkin LO. Chalcone synthase mRNA and activity are reduced in yellow soybean seed coats with dominant I alleles. Plant Physiol. 1994 Jun;105(2):739–748. [PMC free article] [PubMed]
  • Shoemaker Randy, Keim Paul, Vodkin Lila, Retzel Ernest, Clifton Sandra W, Waterston Robert, Smoller David, Coryell Virginia, Khanna Anupama, Erpelding John, et al. A compilation of soybean ESTs: generation and analysis. Genome. 2002 Apr;45(2):329–338. [PubMed]

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