Logo of geneticsGeneticsCurrent IssueInformation for AuthorsEditorial BoardSubscribeSubmit a Manuscript
Genetics. 2002 Nov; 162(3): 1423–1434.
PMCID: PMC1462350

Cold sweetening in diploid potato: mapping quantitative trait loci and candidate genes.


A candidate gene approach has been used as a first step to identify the molecular basis of quantitative trait variation in potato. Sugar content of tubers upon cold storage was the model trait chosen because the metabolic pathways involved in starch and sugar metabolism are well known and many of the genes have been cloned. Tubers of two F(1) populations of diploid potato grown in six environments were evaluated for sugar content after cold storage. The populations were genotyped with RFLP, AFLP, and candidate gene markers. QTL analysis revealed that QTL for glucose, fructose, and sucrose content were located on all potato chromosomes. Most QTL for glucose content mapped to the same positions as QTL for fructose content. QTL explaining >10% of the variability for reducing sugars were located on linkage groups I, III, VII, VIII, IX, and XI. QTL consistent across populations and/or environments were identified. QTL were linked to genes encoding invertase, sucrose synthase 3, sucrose phosphate synthase, ADP-glucose pyrophosphorylase, sucrose transporter 1, and a putative sucrose sensor. The results suggest that allelic variants of enzymes operating in carbohydrate metabolic pathways contribute to the genetic variation in cold sweetening.

Full Text

The Full Text of this article is available as a PDF (192K).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Greiner S, Rausch T, Sonnewald U, Herbers K. Ectopic expression of a tobacco invertase inhibitor homolog prevents cold-induced sweetening of potato tubers. Nat Biotechnol. 1999 Jul;17(7):708–711. [PubMed]
  • Grube RC, Radwanski ER, Jahn M. Comparative genetics of disease resistance within the solanaceae. Genetics. 2000 Jun;155(2):873–887. [PMC free article] [PubMed]
  • Guy CL, Huber JL, Huber SC. Sucrose phosphate synthase and sucrose accumulation at low temperature. Plant Physiol. 1992 Sep;100(1):502–508. [PMC free article] [PubMed]
  • Lander ES, Schork NJ. Genetic dissection of complex traits. Science. 1994 Sep 30;265(5181):2037–2048. [PubMed]
  • Leonards-Schippers C, Gieffers W, Schäfer-Pregl R, Ritter E, Knapp SJ, Salamini F, Gebhardt C. Quantitative resistance to Phytophthora infestans in potato: a case study for QTL mapping in an allogamous plant species. Genetics. 1994 May;137(1):67–77. [PMC free article] [PubMed]
  • Meuwissen TH, Goddard ME. Fine mapping of quantitative trait loci using linkage disequilibria with closely linked marker loci. Genetics. 2000 May;155(1):421–430. [PMC free article] [PubMed]
  • Milbourne D, Meyer RC, Collins AJ, Ramsay LD, Gebhardt C, Waugh R. Isolation, characterisation and mapping of simple sequence repeat loci in potato. Mol Gen Genet. 1998 Aug;259(3):233–245. [PubMed]
  • Bonierbale MW, Plaisted RL, Tanksley SD. RFLP Maps Based on a Common Set of Clones Reveal Modes of Chromosomal Evolution in Potato and Tomato. Genetics. 1988 Dec;120(4):1095–1103. [PMC free article] [PubMed]
  • Ming R, Liu SC, Moore PH, Irvine JE, Paterson AH. QTL analysis in a complex autopolyploid: genetic control of sugar content in sugarcane. Genome Res. 2001 Dec;11(12):2075–2084. [PMC free article] [PubMed]
  • Mitchell-Olds T, Pedersen D. The molecular basis of quantitative genetic variation in central and secondary metabolism in Arabidopsis. Genetics. 1998 Jun;149(2):739–747. [PMC free article] [PubMed]
  • Müller-Röber B, Sonnewald U, Willmitzer L. Inhibition of the ADP-glucose pyrophosphorylase in transgenic potatoes leads to sugar-storing tubers and influences tuber formation and expression of tuber storage protein genes. EMBO J. 1992 Apr;11(4):1229–1238. [PMC free article] [PubMed]
  • Frary A, Nesbitt TC, Grandillo S, Knaap E, Cong B, Liu J, Meller J, Elber R, Alpert KB, Tanksley SD. fw2.2: a quantitative trait locus key to the evolution of tomato fruit size. Science. 2000 Jul 7;289(5476):85–88. [PubMed]
  • Pressey R, Shaw R. Effect of temperature on invertase, invertase inhibitor, and sugars in potato tubers. Plant Physiol. 1966 Dec;41(10):1657–1661. [PMC free article] [PubMed]
  • Ritter E, Gebhardt C, Salamini F. Estimation of recombination frequencies and construction of RFLP linkage maps in plants from crosses between heterozygous parents. Genetics. 1990 Jul;125(3):645–654. [PMC free article] [PubMed]
  • Tanksley SD. Mapping polygenes. Annu Rev Genet. 1993;27:205–233. [PubMed]
  • Van Eck HJ, Jacobs JM, Stam P, Ton J, Stiekema WJ, Jacobsen E. Multiple alleles for tuber shape in diploid potato detected by qualitative and quantitative genetic analysis using RFLPs. Genetics. 1994 May;137(1):303–309. [PMC free article] [PubMed]
  • Vos P, Hogers R, Bleeker M, Reijans M, van de Lee T, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M, et al. AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res. 1995 Nov 11;23(21):4407–4414. [PMC free article] [PubMed]
  • Zrenner R, Salanoubat M, Willmitzer L, Sonnewald U. Evidence of the crucial role of sucrose synthase for sink strength using transgenic potato plants (Solanum tuberosum L.). Plant J. 1995 Jan;7(1):97–107. [PubMed]
  • Talbot CJ, Nicod A, Cherny SS, Fulker DW, Collins AC, Flint J. High-resolution mapping of quantitative trait loci in outbred mice. Nat Genet. 1999 Mar;21(3):305–308. [PubMed]

Articles from Genetics are provided here courtesy of Genetics Society of America


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


  • MedGen
    Related information in MedGen
  • PubMed
    PubMed citations for these articles

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...