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Genetics. 2002 Nov; 162(3): 1415–1422.
PMCID: PMC1462347

Variation in chiasma frequency among eight accessions of Arabidopsis thaliana.


Natural variation in meiotic recombination frequency in Arabidopsis thaliana has been assessed by analyzing chiasma frequency variation among a range of geographically and ecologically diverse accessions. Fifty pollen mother cells at metaphase I of meiosis were analyzed from each of eight accessions and fluorescence in situ hybridization was applied to enable identification of all 10 chromosome arms. There was no significant variation in mean chiasma frequency between plants within accessions, but there was significant variation between accessions. Further analysis confirmed this finding and identified two particular accessions, Cvi and Ler, as having chiasma frequencies significantly lower than those of the other accessions. The analysis also revealed that the pattern of chiasma distribution between arms and among chromosomes is not consistent over accessions. Further detailed analyses were conducted on each individual chromosome (1-5) in turn, revealing that chromosome 4, one of the acrocentric chromosomes, is the least variable while the other acrocentric chromosome (2) is the most variable. These findings indicate the existence of recombination regulatory elements in Arabidopsis and we conclude that it may be possible in the future to identify these elements and determine their mode of action. The practical implications of such developments are considerable.

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  • Wiltfang Joerg, Rupprecht Stephan, Ganslandt Oliver, Nimsky Christopher, Keßler Peter, Schultze-Mosgau Stefan, Fahlbusch Rudolf, Neukam Friedrich Wilhelm. Intraoperative Image-Guided Surgery of the Lateral and Anterior Skull Base in Patients with Tumors or Trauma. Skull Base. 2003 Feb;13(1):21–29. [PMC free article] [PubMed]
  • Alonso-Blanco C, Koornneef M. Naturally occurring variation in Arabidopsis: an underexploited resource for plant genetics. Trends Plant Sci. 2000 Jan;5(1):22–29. [PubMed]
  • Alonso-Blanco C, Peeters AJ, Koornneef M, Lister C, Dean C, van den Bosch N, Pot J, Kuiper MT. Development of an AFLP based linkage map of Ler, Col and Cvi Arabidopsis thaliana ecotypes and construction of a Ler/Cvi recombinant inbred line population. Plant J. 1998 Apr;14(2):259–271. [PubMed]
  • Baker BS, Carpenter AT, Esposito MS, Esposito RE, Sandler L. The genetic control of meiosis. Annu Rev Genet. 1976;10:53–134. [PubMed]
  • Baker SM, Plug AW, Prolla TA, Bronner CE, Harris AC, Yao X, Christie DM, Monell C, Arnheim N, Bradley A, et al. Involvement of mouse Mlh1 in DNA mismatch repair and meiotic crossing over. Nat Genet. 1996 Jul;13(3):336–342. [PubMed]
  • Barth S, Melchinger AE, Devezi-Savula B, Lübberstedt T. A high-throughput system for genome-wide measurement of genetic recombination in Arabidopsis thaliana based on transgenic markers. Funct Integr Genomics. 2000 Nov;1(3):200–206. [PubMed]
  • Caryl AP, Armstrong SJ, Jones GH, Franklin FC. A homologue of the yeast HOP1 gene is inactivated in the Arabidopsis meiotic mutant asy1. Chromosoma. 2000;109(1-2):62–71. [PubMed]
  • Copenhaver GP, Browne WE, Preuss D. Assaying genome-wide recombination and centromere functions with Arabidopsis tetrads. Proc Natl Acad Sci U S A. 1998 Jan 6;95(1):247–252. [PMC free article] [PubMed]
  • Couteau F, Belzile F, Horlow C, Grandjean O, Vezon D, Doutriaux MP. Random chromosome segregation without meiotic arrest in both male and female meiocytes of a dmc1 mutant of Arabidopsis. Plant Cell. 1999 Sep;11(9):1623–1634. [PMC free article] [PubMed]
  • Fransz P, Armstrong S, Alonso-Blanco C, Fischer TC, Torres-Ruiz RA, Jones G. Cytogenetics for the model system Arabidopsis thaliana. Plant J. 1998 Mar;13(6):867–876. [PubMed]
  • Gerlach WL, Bedbrook JR. Cloning and characterization of ribosomal RNA genes from wheat and barley. Nucleic Acids Res. 1979 Dec 11;7(7):1869–1885. [PMC free article] [PubMed]
  • Grelon M, Vezon D, Gendrot G, Pelletier G. AtSPO11-1 is necessary for efficient meiotic recombination in plants. EMBO J. 2001 Feb 1;20(3):589–600. [PMC free article] [PubMed]
  • Latos-Bielenska A, Vogel W. Frequency and distribution of chiasmata in Syrian hamster spermatocytes studied by the BrdU antibody technique. Chromosoma. 1990 Aug;99(4):267–272. [PubMed]
  • Price DJ. Variation in chiasma frequency in Cepaea nemoralis. Heredity (Edinb) 1974 Apr;32(2):211–217. [PubMed]
  • Roeder GS. Meiotic chromosomes: it takes two to tango. Genes Dev. 1997 Oct 15;11(20):2600–2621. [PubMed]
  • Sanchez Moran E, Armstrong SJ, Santos JL, Franklin FC, Jones GH. Chiasma formation in Arabidopsis thaliana accession Wassileskija and in two meiotic mutants. Chromosome Res. 2001;9(2):121–128. [PubMed]
  • Alani E, Subbiah S, Kleckner N. The yeast RAD50 gene encodes a predicted 153-kD protein containing a purine nucleotide-binding domain and two large heptad-repeat regions. Genetics. 1989 May;122(1):47–57. [PMC free article] [PubMed]
  • Sybenga J. Recombination and chiasmata: few but intriguing discrepancies. Genome. 1996 Jun;39(3):473–484. [PubMed]
  • Zickler D, Kleckner N. Meiotic chromosomes: integrating structure and function. Annu Rev Genet. 1999;33:603–754. [PubMed]

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