• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of geneticsGeneticsCurrent IssueInformation for AuthorsEditorial BoardSubscribeSubmit a Manuscript
Genetics. Nov 2002; 162(3): 1275–1282.
PMCID: PMC1462318

Mapping unexplored genomes: a genetic linkage map of the Hawaiian cricket Laupala.

Abstract

As with many organisms of evolutionary interest, the Hawaiian cricket Laupala genome is not well characterized genetically. Mapping such an unexplored genome therefore presents challenges not often faced in model genetic organisms and not well covered in the literature. We discuss the evolutionary merits of Laupala as a model for speciation studies involving prezygotic change, our choice of marker system for detecting genetic variation, and the initial genetic expectations pertaining to the construction of any unknown genomic map in general and to the Laupala linkage map construction in particular. We used the technique of amplified fragment length polymorphism (AFLP) to develop a linkage map of Laupala. We utilized both EcoRI/MseI- and EcoRI/PstI-digested genomic DNA to generate AFLP bands and identified 309 markers that segregated among F(2) interspecific hybrid individuals. The map is composed of 231 markers distributed over 11 and 7 species-specific autosomal groups together with a number of putative X chromosome linkage groups. The integration of codominant markers enabled the identification of five homologous linkage groups corresponding to five of the seven autosomal chromosomal pairs found in Laupala.

Full Text

The Full Text of this article is available as a PDF (132K).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Antolin MF, Bosio CF, Cotton J, Sweeney W, Strand MR, Black WC., 4th Intensive linkage mapping in a wasp (Bracon hebetor) and a mosquito (Aedes aegypti) with single-strand conformation polymorphism analysis of random amplified polymorphic DNA markers. Genetics. 1996 Aug;143(4):1727–1738. [PMC free article] [PubMed]
  • Barton NH, Turelli M. Evolutionary quantitative genetics: how little do we know? Annu Rev Genet. 1989;23:337–370. [PubMed]
  • Beeman RW, Brown SJ. RAPD-based genetic linkage maps of Tribolium castaneum. Genetics. 1999 Sep;153(1):333–338. [PMC free article] [PubMed]
  • Coyne JA, Orr HA. The evolutionary genetics of speciation. Philos Trans R Soc Lond B Biol Sci. 1998 Feb 28;353(1366):287–305. [PMC free article] [PubMed]
  • Gadau J, Page RE, Jr, Werren JH. Mapping of hybrid incompatibility loci in Nasonia. Genetics. 1999 Dec;153(4):1731–1741. [PMC free article] [PubMed]
  • Hawthorne DJ. AFLP-based genetic linkage map of the Colorado potato beetle Leptinotarsa decemlineata: sex chromosomes and a pyrethroid-resistance candidate gene. Genetics. 2001 Jun;158(2):695–700. [PMC free article] [PubMed]
  • Rundle HD, Nagel L, Wenrick Boughman J, Schluter D. Natural selection and parallel speciation in sympatric sticklebacks. Science. 2000 Jan 14;287(5451):306–308. [PubMed]
  • Hawthorne DJ, Via S. Genetic linkage of ecological specialization and reproductive isolation in pea aphids. Nature. 2001 Aug 30;412(6850):904–907. [PubMed]
  • Servedio MR. Reinforcement and the genetics of nonrandom mating. Evolution. 2000 Feb;54(1):21–29. [PubMed]
  • Hunt GJ, Page RE., Jr Linkage map of the honey bee, Apis mellifera, based on RAPD markers. Genetics. 1995 Mar;139(3):1371–1382. [PMC free article] [PubMed]
  • Jiang C, Zeng ZB. Mapping quantitative trait loci with dominant and missing markers in various crosses from two inbred lines. Genetica. 1997;101(1):47–58. [PubMed]
  • Kim SC, Rieseberg LH. Genetic architecture of species differences in annual sunflowers: implications for adaptive trait introgression. Genetics. 1999 Oct;153(2):965–977. [PMC free article] [PubMed]
  • Shaw KL. Interspecific genetics of mate recognition: inheritance of female acoustic preference in Hawaiian crickets. Evolution. 2000 Aug;54(4):1303–1312. [PubMed]
  • Kocher TD, Lee WJ, Sobolewska H, Penman D, McAndrew B. A genetic linkage map of a cichlid fish, the tilapia (Oreochromis niloticus). Genetics. 1998 Mar;148(3):1225–1232. [PMC free article] [PubMed]
  • Shaw Kerry L, Parsons Yvonne M. Divergence of mate recognition behavior and its consequences for genetic architectures of speciation. Am Nat. 2002 Mar;159 (Suppl 3):S61–S75. [PubMed]
  • Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newberg LA, Newburg L. MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics. 1987 Oct;1(2):174–181. [PubMed]
  • Laurent V, Wajnberg E, Mangin B, Schiex T, Gaspin C, Vanlerberghe-Masutti F. A composite genetic map of the parasitoid wasp Trichogramma brassicae based on RAPD markers. Genetics. 1998 Sep;150(1):275–282. [PMC free article] [PubMed]
  • Mackill DJ, Zhang Z, Redoña ED, Colowit PM. Level of polymorphism and genetic mapping of AFLP markers in rice. Genome. 1996 Oct;39(5):969–977. [PubMed]
  • Vos P, Hogers R, Bleeker M, Reijans M, van de Lee T, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M, et al. AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res. 1995 Nov 11;23(21):4407–4414. [PMC free article] [PubMed]
  • Naruse K, Fukamachi S, Mitani H, Kondo M, Matsuoka T, Kondo S, Hanamura N, Morita Y, Hasegawa K, Nishigaki R, et al. A detailed linkage map of medaka, Oryzias latipes: comparative genomics and genome evolution. Genetics. 2000 Apr;154(4):1773–1784. [PMC free article] [PubMed]
  • Parsons YM, Shaw KL. Species boundaries and genetic diversity among Hawaiian crickets of the genus Laupala identified using amplified fragment length polymorphism. Mol Ecol. 2001 Jul;10(7):1765–1772. [PubMed]
  • Wu CI, Palopoli MF. Genetics of postmating reproductive isolation in animals. Annu Rev Genet. 1994;28:283–308. [PubMed]
  • Xie C, Gessler DD, Xu S. Combining different line crosses for mapping quantitative trait loci using the identical by descent-based variance component method. Genetics. 1998 Jun;149(2):1139–1146. [PMC free article] [PubMed]
  • Petrov DA, Sangster TA, Johnston JS, Hartl DL, Shaw KL. Evidence for DNA loss as a determinant of genome size. Science. 2000 Feb 11;287(5455):1060–1062. [PubMed]
  • Yasukochi Y. A dense genetic map of the silkworm, Bombyx mori, covering all chromosomes based on 1018 molecular markers. Genetics. 1998 Dec;150(4):1513–1525. [PMC free article] [PubMed]

Articles from Genetics are provided here courtesy of Genetics Society of America

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...