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Genetics. Nov 2002; 162(3): 1445–1456.
PMCID: PMC1462317

Three redundant brassinosteroid early response genes encode putative bHLH transcription factors required for normal growth.

Abstract

Brassinosteroids (BRs) are a class of polyhydroxylated steroids that are important regulators of plant growth and development. We have identified three closely related basic helix-loop-helix (bHLH) transcription factors, BEE1, BEE2, and BEE3, as products of early response genes required for full BR response. Comparison of the phenotypes of plants that overexpress BEE1 with bee1 bee2 bee3 triple-knockout mutant plants suggests that BEE1, BEE2, and BEE3 are functionally redundant positive regulators of BR signaling. Expression of BEE1, BEE2, and BEE3 is also regulated by other hormones, notably abscisic acid (ABA), a known antagonist of BR signaling. Reduced ABA response in plants overexpressing BEE1 suggests that BEE proteins may function as signaling intermediates in multiple pathways.

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Selected References

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  • Choe S, Fujioka S, Noguchi T, Takatsuto S, Yoshida S, Feldmann KA. Overexpression of DWARF4 in the brassinosteroid biosynthetic pathway results in increased vegetative growth and seed yield in Arabidopsis. Plant J. 2001 Jun;26(6):573–582. [PubMed]
  • Clack T, Mathews S, Sharrock RA. The phytochrome apoprotein family in Arabidopsis is encoded by five genes: the sequences and expression of PHYD and PHYE. Plant Mol Biol. 1994 Jun;25(3):413–427. [PubMed]
  • Clouse SD, Langford M, McMorris TC. A brassinosteroid-insensitive mutant in Arabidopsis thaliana exhibits multiple defects in growth and development. Plant Physiol. 1996 Jul;111(3):671–678. [PMC free article] [PubMed]
  • Cutler S, Ghassemian M, Bonetta D, Cooney S, McCourt P. A protein farnesyl transferase involved in abscisic acid signal transduction in Arabidopsis. Science. 1996 Aug 30;273(5279):1239–1241. [PubMed]
  • Devlin PF, Patel SR, Whitelam GC. Phytochrome E influences internode elongation and flowering time in Arabidopsis. Plant Cell. 1998 Sep;10(9):1479–1487. [PMC free article] [PubMed]
  • Devlin PF, Robson PR, Patel SR, Goosey L, Sharrock RA, Whitelam GC. Phytochrome D acts in the shade-avoidance syndrome in Arabidopsis by controlling elongation growth and flowering time. Plant Physiol. 1999 Mar;119(3):909–915. [PMC free article] [PubMed]
  • Ephritikhine G, Fellner M, Vannini C, Lapous D, Barbier-Brygoo H. The sax1 dwarf mutant of Arabidopsis thaliana shows altered sensitivity of growth responses to abscisic acid, auxin, gibberellins and ethylene and is partially rescued by exogenous brassinosteroid. Plant J. 1999 May;18(3):303–314. [PubMed]
  • Fairchild CD, Schumaker MA, Quail PH. HFR1 encodes an atypical bHLH protein that acts in phytochrome A signal transduction. Genes Dev. 2000 Sep 15;14(18):2377–2391. [PMC free article] [PubMed]
  • Friedrichsen DM, Joazeiro CA, Li J, Hunter T, Chory J. Brassinosteroid-insensitive-1 is a ubiquitously expressed leucine-rich repeat receptor serine/threonine kinase. Plant Physiol. 2000 Aug;123(4):1247–1256. [PMC free article] [PubMed]
  • Ghassemian M, Nambara E, Cutler S, Kawaide H, Kamiya Y, McCourt P. Regulation of abscisic acid signaling by the ethylene response pathway in Arabidopsis. Plant Cell. 2000 Jul;12(7):1117–1126. [PMC free article] [PubMed]
  • Hajdukiewicz P, Svab Z, Maliga P. The small, versatile pPZP family of Agrobacterium binary vectors for plant transformation. Plant Mol Biol. 1994 Sep;25(6):989–994. [PubMed]
  • He Jun-Xian, Gendron Joshua M, Yang Yanli, Li Jianming, Wang Zhi-Yong. The GSK3-like kinase BIN2 phosphorylates and destabilizes BZR1, a positive regulator of the brassinosteroid signaling pathway in Arabidopsis. Proc Natl Acad Sci U S A. 2002 Jul 23;99(15):10185–10190. [PMC free article] [PubMed]
  • Heisler MG, Atkinson A, Bylstra YH, Walsh R, Smyth DR. SPATULA, a gene that controls development of carpel margin tissues in Arabidopsis, encodes a bHLH protein. Development. 2001 Apr;128(7):1089–1098. [PubMed]
  • Abe H, Yamaguchi-Shinozaki K, Urao T, Iwasaki T, Hosokawa D, Shinozaki K. Role of arabidopsis MYC and MYB homologs in drought- and abscisic acid-regulated gene expression. Plant Cell. 1997 Oct;9(10):1859–1868. [PMC free article] [PubMed]
  • Hu Y, Bao F, Li J. Promotive effect of brassinosteroids on cell division involves a distinct CycD3-induction pathway in Arabidopsis. Plant J. 2000 Dec;24(5):693–701. [PubMed]
  • Abel S, Nguyen MD, Theologis A. The PS-IAA4/5-like family of early auxin-inducible mRNAs in Arabidopsis thaliana. J Mol Biol. 1995 Aug 25;251(4):533–549. [PubMed]
  • Analysis of the genome sequence of the flowering plant Arabidopsis thaliana. Nature. 2000 Dec 14;408(6814):796–815. [PubMed]
  • Kieber JJ, Rothenberg M, Roman G, Feldmann KA, Ecker JR. CTR1, a negative regulator of the ethylene response pathway in Arabidopsis, encodes a member of the raf family of protein kinases. Cell. 1993 Feb 12;72(3):427–441. [PubMed]
  • Kuno N, Muramatsu T, Hamazato F, Furuya M. Identification by large-scale screening of phytochrome-regulated genes in etiolated seedlings of Arabidopsis using a fluorescent differential display technique. Plant Physiol. 2000 Jan;122(1):15–24. [PMC free article] [PubMed]
  • Lee MM, Schiefelbein J. Developmentally distinct MYB genes encode functionally equivalent proteins in Arabidopsis. Development. 2001 May;128(9):1539–1546. [PubMed]
  • Choe S, Noguchi T, Fujioka S, Takatsuto S, Tissier CP, Gregory BD, Ross AS, Tanaka A, Yoshida S, Tax FE, et al. The Arabidopsis dwf7/ste1 mutant is defective in the delta7 sterol C-5 desaturation step leading to brassinosteroid biosynthesis. Plant Cell. 1999 Feb;11(2):207–221. [PMC free article] [PubMed]
  • Li J, Chory J. A putative leucine-rich repeat receptor kinase involved in brassinosteroid signal transduction. Cell. 1997 Sep 5;90(5):929–938. [PubMed]
  • Li Jianming, Nam Kyoung Hee. Regulation of brassinosteroid signaling by a GSK3/SHAGGY-like kinase. Science. 2002 Feb 15;295(5558):1299–1301. [PubMed]
  • Li J, Nagpal P, Vitart V, McMorris TC, Chory J. A role for brassinosteroids in light-dependent development of Arabidopsis. Science. 1996 Apr 19;272(5260):398–401. [PubMed]
  • Li J, Lease KA, Tax FE, Walker JC. BRS1, a serine carboxypeptidase, regulates BRI1 signaling in Arabidopsis thaliana. Proc Natl Acad Sci U S A. 2001 May 8;98(10):5916–5921. [PMC free article] [PubMed]
  • Soh MS, Kim YM, Han SJ, Song PS. REP1, a basic helix-loop-helix protein, is required for a branch pathway of phytochrome A signaling in arabidopsis. Plant Cell. 2000 Nov;12(11):2061–2074. [PMC free article] [PubMed]
  • Spiegelman JI, Mindrinos MN, Fankhauser C, Richards D, Lutes J, Chory J, Oefner PJ. Cloning of the Arabidopsis RSF1 gene by using a mapping strategy based on high-density DNA arrays and denaturing high-performance liquid chromatography. Plant Cell. 2000 Dec;12(12):2485–2498. [PMC free article] [PubMed]
  • Li Jia, Wen Jiangqi, Lease Kevin A, Doke Jason T, Tax Frans E, Walker John C. BAK1, an Arabidopsis LRR receptor-like protein kinase, interacts with BRI1 and modulates brassinosteroid signaling. Cell. 2002 Jul 26;110(2):213–222. [PubMed]
  • Steber CM, McCourt P. A role for brassinosteroids in germination in Arabidopsis. Plant Physiol. 2001 Feb;125(2):763–769. [PMC free article] [PubMed]
  • Massari ME, Murre C. Helix-loop-helix proteins: regulators of transcription in eucaryotic organisms. Mol Cell Biol. 2000 Jan;20(2):429–440. [PMC free article] [PubMed]
  • Szekeres M, Németh K, Koncz-Kálmán Z, Mathur J, Kauschmann A, Altmann T, Rédei GP, Nagy F, Schell J, Koncz C. Brassinosteroids rescue the deficiency of CYP90, a cytochrome P450, controlling cell elongation and de-etiolation in Arabidopsis. Cell. 1996 Apr 19;85(2):171–182. [PubMed]
  • Mathur J, Molnár G, Fujioka S, Takatsuto S, Sakurai A, Yokota T, Adam G, Voigt B, Nagy F, Maas C, et al. Transcription of the Arabidopsis CPD gene, encoding a steroidogenic cytochrome P450, is negatively controlled by brassinosteroids. Plant J. 1998 Jun;14(5):593–602. [PubMed]
  • Thompson JD, Higgins DG, Gibson TJ. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 1994 Nov 11;22(22):4673–4680. [PMC free article] [PubMed]
  • Muñoz FJ, Labrador E, Dopico B. Brassinolides promote the expression of a new Cicer arietinum beta-tubulin gene involved in the epicotyl elongation. Plant Mol Biol. 1998 Jul;37(5):807–817. [PubMed]
  • Wang ZY, Seto H, Fujioka S, Yoshida S, Chory J. BRI1 is a critical component of a plasma-membrane receptor for plant steroids. Nature. 2001 Mar 15;410(6826):380–383. [PubMed]
  • Nam Kyoung Hee, Li Jianming. BRI1/BAK1, a receptor kinase pair mediating brassinosteroid signaling. Cell. 2002 Jul 26;110(2):203–212. [PubMed]
  • Wang Zhi Yong, Nakano Takeshi, Gendron Joshua, He Junxian, Chen Meng, Vafeados Dionne, Yang Yanli, Fujioka Shozo, Yoshida Shigeo, Asami Tadao, et al. Nuclear-localized BZR1 mediates brassinosteroid-induced growth and feedback suppression of brassinosteroid biosynthesis. Dev Cell. 2002 Apr;2(4):505–513. [PubMed]
  • Neff MM, Nguyen SM, Malancharuvil EJ, Fujioka S, Noguchi T, Seto H, Tsubuki M, Honda T, Takatsuto S, Yoshida S, et al. BAS1: A gene regulating brassinosteroid levels and light responsiveness in Arabidopsis. Proc Natl Acad Sci U S A. 1999 Dec 21;96(26):15316–15323. [PMC free article] [PubMed]
  • Xu W, Campbell P, Vargheese AK, Braam J. The Arabidopsis XET-related gene family: environmental and hormonal regulation of expression. Plant J. 1996 Jun;9(6):879–889. [PubMed]
  • Ni M, Tepperman JM, Quail PH. PIF3, a phytochrome-interacting factor necessary for normal photoinduced signal transduction, is a novel basic helix-loop-helix protein. Cell. 1998 Nov 25;95(5):657–667. [PubMed]
  • Yi HC, Joo S, Nam KH, Lee JS, Kang BG, Kim WT. Auxin and brassinosteroid differentially regulate the expression of three members of the 1-aminocyclopropane-1-carboxylate synthase gene family in mung bean (Vigna radiata L.). Plant Mol Biol. 1999 Nov;41(4):443–454. [PubMed]
  • Nicol F, His I, Jauneau A, Vernhettes S, Canut H, Höfte H. A plasma membrane-bound putative endo-1,4-beta-D-glucanase is required for normal wall assembly and cell elongation in Arabidopsis. EMBO J. 1998 Oct 1;17(19):5563–5576. [PMC free article] [PubMed]
  • Yin Yanhai, Wang Zhi Yong, Mora-Garcia Santiago, Li Jianming, Yoshida Shigeo, Asami Tadao, Chory Joanne. BES1 accumulates in the nucleus in response to brassinosteroids to regulate gene expression and promote stem elongation. Cell. 2002 Apr 19;109(2):181–191. [PubMed]
  • Noguchi T, Fujioka S, Choe S, Takatsuto S, Yoshida S, Yuan H, Feldmann KA, Tax FE. Brassinosteroid-insensitive dwarf mutants of Arabidopsis accumulate brassinosteroids. Plant Physiol. 1999 Nov;121(3):743–752. [PMC free article] [PubMed]
  • Yoshizumi T, Nagata N, Shimada H, Matsui M. An Arabidopsis cell cycle -dependent kinase-related gene, CDC2b, plays a role in regulating seedling growth in darkness. Plant Cell. 1999 Oct;11(10):1883–1896. [PMC free article] [PubMed]
  • Oh MH, Ray WK, Huber SC, Asara JM, Gage DA, Clouse SD. Recombinant brassinosteroid insensitive 1 receptor-like kinase autophosphorylates on serine and threonine residues and phosphorylates a conserved peptide motif in vitro. Plant Physiol. 2000 Oct;124(2):751–766. [PMC free article] [PubMed]
  • Zhao Y, Christensen SK, Fankhauser C, Cashman JR, Cohen JD, Weigel D, Chory J. A role for flavin monooxygenase-like enzymes in auxin biosynthesis. Science. 2001 Jan 12;291(5502):306–309. [PubMed]
  • Zurek DM, Clouse SD. Molecular cloning and characterization of a brassinosteroid-regulated gene from elongating soybean (Glycine max L.) epicotyls. Plant Physiol. 1994 Jan;104(1):161–170. [PMC free article] [PubMed]
  • Pelaz S, Ditta GS, Baumann E, Wisman E, Yanofsky MF. B and C floral organ identity functions require SEPALLATA MADS-box genes. Nature. 2000 May 11;405(6783):200–203. [PubMed]

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