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Genetics. Oct 2002; 162(2): 767–780.
PMCID: PMC1462291

Exploring the envelope. Systematic alteration in the sex-determination system of the nematode caenorhabditis elegans.

Abstract

The natural sexes of the nematode Caenorhabditis elegans are the self-fertilizing hermaphrodite (XX) and the male (XO). The underlying genetic pathway controlling sexual phenotype has been extensively investigated. Mutations in key regulatory genes have been used to create a series of stable populations in which sex is determined not by X chromosome dosage, but in a variety of other ways, many of which mimic the diverse sex-determination systems found in different animal species. Most of these artificial strains have male and female sexes. Each of seven autosomal genes can be made to adopt a role as the primary determinant of sex, and each of the five autosomes can carry the primary determinant, thereby becoming a sex chromosome. Strains with sex determination by fragment chromosomes, episomes, compound chromosomes, or environmental factors have also been constructed. The creation of these strains demonstrates the ease with which one sex-determination system can be transformed into another.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Ahmed S, Hodgkin J. MRT-2 checkpoint protein is required for germline immortality and telomere replication in C. elegans. Nature. 2000 Jan 13;403(6766):159–164. [PubMed]
  • Ahringer J, Rosenquist TA, Lawson DN, Kimble J. The Caenorhabditis elegans sex determining gene fem-3 is regulated post-transcriptionally. EMBO J. 1992 Jun;11(6):2303–2310. [PMC free article] [PubMed]
  • Akerib CC, Meyer BJ. Identification of X chromosome regions in Caenorhabditis elegans that contain sex-determination signal elements. Genetics. 1994 Dec;138(4):1105–1125. [PMC free article] [PubMed]
  • Ashworth A, Rastan S, Lovell-Badge R, Kay G. X-chromosome inactivation may explain the difference in viability of XO humans and mice. Nature. 1991 May 30;351(6325):406–408. [PubMed]
  • Bandi C, Anderson TJ, Genchi C, Blaxter ML. Phylogeny of Wolbachia in filarial nematodes. Proc Biol Sci. 1998 Dec 22;265(1413):2407–2413. [PMC free article] [PubMed]
  • Bandi C, Dunn AM, Hurst GD, Rigaud T. Inherited microorganisms, sex-specific virulence and reproductive parasitism. Trends Parasitol. 2001 Feb;17(2):88–94. [PubMed]
  • Hodgkin J, Zellan JD, Albertson DG. Identification of a candidate primary sex determination locus, fox-1, on the X chromosome of Caenorhabditis elegans. Development. 1994 Dec;120(12):3681–3689. [PubMed]
  • Horvitz HR, Brenner S, Hodgkin J, Herman RK. A uniform genetic nomenclature for the nematode Caenorhabditis elegans. Mol Gen Genet. 1979 Sep;175(2):129–133. [PubMed]
  • Beukeboom LW. Sex determination in Hymenoptera: a need for genetic and molecular studies. Bioessays. 1995 Sep;17(9):813–817. [PubMed]
  • Klass M, Wolf N, Hirsh D. Development of the male reproductive system and sexual transformation in the nematode Caenorhabditis elegans. Dev Biol. 1976 Aug;52(1):1–18. [PubMed]
  • Brenner S. The genetics of Caenorhabditis elegans. Genetics. 1974 May;77(1):71–94. [PMC free article] [PubMed]
  • Kondo K, Makovec B, Waterston RH, Hodgkin J. Genetic and molecular analysis of eight tRNA(Trp) amber suppressors in Caenorhabditis elegans. J Mol Biol. 1990 Sep 5;215(1):7–19. [PubMed]
  • Carmi I, Meyer BJ. The primary sex determination signal of Caenorhabditis elegans. Genetics. 1999 Jul;152(3):999–1015. [PMC free article] [PubMed]
  • Kuwabara PE. A novel regulatory mutation in the C. elegans sex determination gene tra-2 defines a candidate ligand/receptor interaction site. Development. 1996 Jul;122(7):2089–2098. [PubMed]
  • Cline TW, Meyer BJ. Vive la différence: males vs females in flies vs worms. Annu Rev Genet. 1996;30:637–702. [PubMed]
  • Dawes HE, Berlin DS, Lapidus DM, Nusbaum C, Davis TL, Meyer BJ. Dosage compensation proteins targeted to X chromosomes by a determinant of hermaphrodite fate. Science. 1999 Jun 11;284(5421):1800–1804. [PubMed]
  • Kuwabara PE. Developmental genetics of Caenorhabditis elegans sex determination. Curr Top Dev Biol. 1999;41:99–132. [PubMed]
  • de Bono M, Hodgkin J. Evolution of sex determination in caenorhabditis: unusually high divergence of tra-1 and its functional consequences. Genetics. 1996 Oct;144(2):587–595. [PMC free article] [PubMed]
  • Kuwabara PE, Perry MD. It ain't over till it's ova: germline sex determination in C. elegans. Bioessays. 2001 Jul;23(7):596–604. [PubMed]
  • Kuwabara PE, Okkema PG, Kimble J. tra-2 encodes a membrane protein and may mediate cell communication in the Caenorhabditis elegans sex determination pathway. Mol Biol Cell. 1992 Apr;3(4):461–473. [PMC free article] [PubMed]
  • Liu WS, Nordqvist K, Lau YF, Fredga K. Characterization of the Xp21-23 region in the wood lemming, a region involved in XY sex reversal. J Exp Zool. 2001 Nov 1;290(6):551–557. [PubMed]
  • Lum DH, Kuwabara PE, Zarkower D, Spence AM. Direct protein-protein interaction between the intracellular domain of TRA-2 and the transcription factor TRA-1A modulates feminizing activity in C. elegans. Genes Dev. 2000 Dec 15;14(24):3153–3165. [PMC free article] [PubMed]
  • Madl JE, Herman RK. Polyploids and sex determination in Caenorhabditis elegans. Genetics. 1979 Oct;93(2):393–402. [PMC free article] [PubMed]
  • Gibbs HL, Sorenson MD, Marchetti K, Brooke MD, Davies NB, Nakamura H. Genetic evidence for female host-specific races of the common cuckoo. Nature. 2000 Sep 14;407(6801):183–186. [PubMed]
  • Mehra A, Gaudet J, Heck L, Kuwabara PE, Spence AM. Negative regulation of male development in Caenorhabditis elegans by a protein-protein interaction between TRA-2A and FEM-3. Genes Dev. 1999 Jun 1;13(11):1453–1463. [PMC free article] [PubMed]
  • Goodwin Elizabeth B, Ellis Ronald E. Turning clustering loops: sex determination in Caenorhabditis elegans. Curr Biol. 2002 Feb 5;12(3):R111–R120. [PubMed]
  • Miller LM, Plenefisch JD, Casson LP, Meyer BJ. xol-1: a gene that controls the male modes of both sex determination and X chromosome dosage compensation in C. elegans. Cell. 1988 Oct 7;55(1):167–183. [PubMed]
  • Goodwin EB, Hofstra K, Hurney CA, Mango S, Kimble J. A genetic pathway for regulation of tra-2 translation. Development. 1997 Feb;124(3):749–758. [PubMed]
  • Haack H, Hodgkin J. Tests for parental imprinting in the nematode Caenorhabditis elegans. Mol Gen Genet. 1991 Sep;228(3):482–485. [PubMed]
  • O'Neil MT, Belote JM. Interspecific comparison of the transformer gene of Drosophila reveals an unusually high degree of evolutionary divergence. Genetics. 1992 May;131(1):113–128. [PMC free article] [PubMed]
  • Hodgkin J. Two types of sex determination in a nematode. Nature. 1983 Jul 21;304(5923):267–268. [PubMed]
  • Peckol EL, Troemel ER, Bargmann CI. Sensory experience and sensory activity regulate chemosensory receptor gene expression in Caenorhabditis elegans. Proc Natl Acad Sci U S A. 2001 Sep 25;98(20):11032–11038. [PMC free article] [PubMed]
  • Perry MD, Li W, Trent C, Robertson B, Fire A, Hageman JM, Wood WB. Molecular characterization of the her-1 gene suggests a direct role in cell signaling during Caenorhabditis elegans sex determination. Genes Dev. 1993 Feb;7(2):216–228. [PubMed]
  • Hodgkin J. A genetic analysis of the sex-determining gene, tra-1, in the nematode Caenorhabditis elegans. Genes Dev. 1987 Sep;1(7):731–745. [PubMed]
  • Pilgrim D, McGregor A, Jäckle P, Johnson T, Hansen D. The C. elegans sex-determining gene fem-2 encodes a putative protein phosphatase. Mol Biol Cell. 1995 Sep;6(9):1159–1171. [PMC free article] [PubMed]
  • Plenefisch JD, DeLong L, Meyer BJ. Genes that implement the hermaphrodite mode of dosage compensation in Caenorhabditis elegans. Genetics. 1989 Jan;121(1):57–76. [PMC free article] [PubMed]
  • Raymond CS, Shamu CE, Shen MM, Seifert KJ, Hirsch B, Hodgkin J, Zarkower D. Evidence for evolutionary conservation of sex-determining genes. Nature. 1998 Feb 12;391(6668):691–695. [PubMed]
  • Hodgkin J, Albertson DG. Isolation of dominant XO-feminizing mutations in Caenorhabditis elegans: new regulatory tra alleles and an X chromosome duplication with implications for primary sex determination. Genetics. 1995 Oct;141(2):527–542. [PMC free article] [PubMed]
  • Rosenbluth RE, Baillie DL. The genetic analysis of a reciprocal translocation, eT1(III; V), in Caenorhabditis elegans. Genetics. 1981 Nov-Dec;99(3-4):415–428. [PMC free article] [PubMed]
  • Schedl T, Kimble J. fog-2, a germ-line-specific sex determination gene required for hermaphrodite spermatogenesis in Caenorhabditis elegans. Genetics. 1988 May;119(1):43–61. [PMC free article] [PubMed]
  • Schierenberg E, Wood WB. Control of cell-cycle timing in early embryos of Caenorhabditis elegans. Dev Biol. 1985 Feb;107(2):337–354. [PubMed]
  • Schütt C, Nöthiger R. Structure, function and evolution of sex-determining systems in Dipteran insects. Development. 2000 Feb;127(4):667–677. [PubMed]
  • Mark Welch D, Meselson M. Evidence for the evolution of bdelloid rotifers without sexual reproduction or genetic exchange. Science. 2000 May 19;288(5469):1211–1215. [PubMed]
  • Sigurdson DC, Herman RK, Horton CA, Kari CK, Pratt SE. An X-autosome fusion chromosome of Caenorhabditis elegans. Mol Gen Genet. 1986 Feb;202(2):212–218. [PubMed]
  • Whitfield LS, Lovell-Badge R, Goodfellow PN. Rapid sequence evolution of the mammalian sex-determining gene SRY. Nature. 1993 Aug 19;364(6439):713–715. [PubMed]
  • Sokol SB, Kuwabara PE. Proteolysis in Caenorhabditis elegans sex determination: cleavage of TRA-2A by TRA-3. Genes Dev. 2000 Apr 15;14(8):901–906. [PMC free article] [PubMed]
  • Zarkower D. Establishing sexual dimorphism: conservation amidst diversity? Nat Rev Genet. 2001 Mar;2(3):175–185. [PubMed]
  • Zarkower D, Hodgkin J. Molecular analysis of the C. elegans sex-determining gene tra-1: a gene encoding two zinc finger proteins. Cell. 1992 Jul 24;70(2):237–249. [PubMed]
  • Spence AM, Coulson A, Hodgkin J. The product of fem-1, a nematode sex-determining gene, contains a motif found in cell cycle control proteins and receptors for cell-cell interactions. Cell. 1990 Mar 23;60(6):981–990. [PubMed]
  • Zarkower D, De Bono M, Aronoff R, Hodgkin J. Regulatory rearrangements and smg-sensitive alleles of the C. elegans sex-determining gene tra-1. Dev Genet. 1994;15(3):240–250. [PubMed]
  • Trent C, Wood WB, Horvitz HR. A novel dominant transformer allele of the sex-determining gene her-1 of Caenorhabditis elegans. Genetics. 1988 Sep;120(1):145–157. [PMC free article] [PubMed]

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