• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of geneticsGeneticsCurrent IssueInformation for AuthorsEditorial BoardSubscribeSubmit a Manuscript
Genetics. Sep 2002; 162(1): 113–128.
PMCID: PMC1462232

A targeted RNAi screen for genes involved in chromosome morphogenesis and nuclear organization in the Caenorhabditis elegans germline.

Abstract

We have implemented a functional genomics strategy to identify genes involved in chromosome morphogenesis and nuclear organization during meiotic prophase in the Caenorhabditis elegans germline. This approach took advantage of a gene-expression survey that used DNA microarray technology to identify genes preferentially expressed in the germline. We defined a subset of 192 germline-enriched genes whose expression profiles were similar to those of previously identified meiosis genes and designed a screen to identify genes for which inhibition by RNA interference (RNAi) elicited defects in function or development of the germline. We obtained strong germline phenotypes for 27% of the genes tested, indicating that this targeted approach greatly enriched for genes that function in the germline. In addition to genes involved in key meiotic prophase events, we identified genes involved in meiotic progression, germline proliferation, and chromosome organization and/or segregation during mitotic growth.

Full Text

The Full Text of this article is available as a PDF (204K).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Albertson DG, Sulston JE, White JG. Cell cycling and DNA replication in a mutant blocked in cell division in the nematode Caenorhabditis elegans. Dev Biol. 1978 Mar;63(1):165–178. [PubMed]
  • Bai C, Sen P, Hofmann K, Ma L, Goebl M, Harper JW, Elledge SJ. SKP1 connects cell cycle regulators to the ubiquitin proteolysis machinery through a novel motif, the F-box. Cell. 1996 Jul 26;86(2):263–274. [PubMed]
  • Beanan MJ, Strome S. Characterization of a germ-line proliferation mutation in C. elegans. Development. 1992 Nov;116(3):755–766. [PubMed]
  • Berger B, Wilson DB, Wolf E, Tonchev T, Milla M, Kim PS. Predicting coiled coils by use of pairwise residue correlations. Proc Natl Acad Sci U S A. 1995 Aug 29;92(18):8259–8263. [PMC free article] [PubMed]
  • Berry LW, Westlund B, Schedl T. Germ-line tumor formation caused by activation of glp-1, a Caenorhabditis elegans member of the Notch family of receptors. Development. 1997 Feb;124(4):925–936. [PubMed]
  • Blelloch R, Kimble J. Control of organ shape by a secreted metalloprotease in the nematode Caenorhabditis elegans. Nature. 1999 Jun 10;399(6736):586–590. [PubMed]
  • Brenner S. The genetics of Caenorhabditis elegans. Genetics. 1974 May;77(1):71–94. [PMC free article] [PubMed]
  • Genome sequence of the nematode C. elegans: a platform for investigating biology. Science. 1998 Dec 11;282(5396):2012–2018. [PubMed]
  • Chin GM, Villeneuve AM. C. elegans mre-11 is required for meiotic recombination and DNA repair but is dispensable for the meiotic G(2) DNA damage checkpoint. Genes Dev. 2001 Mar 1;15(5):522–534. [PMC free article] [PubMed]
  • Church DL, Guan KL, Lambie EJ. Three genes of the MAP kinase cascade, mek-2, mpk-1/sur-1 and let-60 ras, are required for meiotic cell cycle progression in Caenorhabditis elegans. Development. 1995 Aug;121(8):2525–2535. [PubMed]
  • Cohen-Fix O. The making and breaking of sister chromatid cohesion. Cell. 2001 Jul 27;106(2):137–140. [PubMed]
  • Dernburg AF, McDonald K, Moulder G, Barstead R, Dresser M, Villeneuve AM. Meiotic recombination in C. elegans initiates by a conserved mechanism and is dispensable for homologous chromosome synapsis. Cell. 1998 Aug 7;94(3):387–398. [PubMed]
  • Dernburg AF, Zalevsky J, Colaiácovo MP, Villeneuve AM. Transgene-mediated cosuppression in the C. elegans germ line. Genes Dev. 2000 Jul 1;14(13):1578–1583. [PMC free article] [PubMed]
  • MacQueen AJ, Villeneuve AM. Nuclear reorganization and homologous chromosome pairing during meiotic prophase require C. elegans chk-2. Genes Dev. 2001 Jul 1;15(13):1674–1687. [PMC free article] [PubMed]
  • Endow SA, Henikoff S, Soler-Niedziela L. Mediation of meiotic and early mitotic chromosome segregation in Drosophila by a protein related to kinesin. Nature. 1990 May 3;345(6270):81–83. [PubMed]
  • Maeda I, Kohara Y, Yamamoto M, Sugimoto A. Large-scale analysis of gene function in Caenorhabditis elegans by high-throughput RNAi. Curr Biol. 2001 Feb 6;11(3):171–176. [PubMed]
  • Flores-Rozas H, Kolodner RD. Links between replication, recombination and genome instability in eukaryotes. Trends Biochem Sci. 2000 Apr;25(4):196–200. [PMC free article] [PubMed]
  • Matthies HJ, McDonald HB, Goldstein LS, Theurkauf WE. Anastral meiotic spindle morphogenesis: role of the non-claret disjunctional kinesin-like protein. J Cell Biol. 1996 Jul;134(2):455–464. [PMC free article] [PubMed]
  • Francis R, Barton MK, Kimble J, Schedl T. gld-1, a tumor suppressor gene required for oocyte development in Caenorhabditis elegans. Genetics. 1995 Feb;139(2):579–606. [PMC free article] [PubMed]
  • McDonald HB, Goldstein LS. Identification and characterization of a gene encoding a kinesin-like protein in Drosophila. Cell. 1990 Jun 15;61(6):991–1000. [PubMed]
  • Fraser AG, Kamath RS, Zipperlen P, Martinez-Campos M, Sohrmann M, Ahringer J. Functional genomic analysis of C. elegans chromosome I by systematic RNA interference. Nature. 2000 Nov 16;408(6810):325–330. [PubMed]
  • Meluh PB, Rose MD. KAR3, a kinesin-related gene required for yeast nuclear fusion. Cell. 1990 Mar 23;60(6):1029–1041. [PubMed]
  • O'Donnell M, Jeruzalmi D, Kuriyan J. Clamp loader structure predicts the architecture of DNA polymerase III holoenzyme and RFC. Curr Biol. 2001 Nov 13;11(22):R935–R946. [PubMed]
  • Friedman L, Santa Anna-Arriola S, Hodgkin J, Kimble J. gon-4, a cell lineage regulator required for gonadogenesis in Caenorhabditis elegans. Dev Biol. 2000 Dec 15;228(2):350–362. [PubMed]
  • Oegema K, Desai A, Rybina S, Kirkham M, Hyman AA. Functional analysis of kinetochore assembly in Caenorhabditis elegans. J Cell Biol. 2001 Jun 11;153(6):1209–1226. [PMC free article] [PubMed]
  • Gartner A, Milstein S, Ahmed S, Hodgkin J, Hengartner MO. A conserved checkpoint pathway mediates DNA damage--induced apoptosis and cell cycle arrest in C. elegans. Mol Cell. 2000 Mar;5(3):435–443. [PubMed]
  • Pasierbek P, Jantsch M, Melcher M, Schleiffer A, Schweizer D, Loidl J. A Caenorhabditis elegans cohesion protein with functions in meiotic chromosome pairing and disjunction. Genes Dev. 2001 Jun 1;15(11):1349–1360. [PMC free article] [PubMed]
  • Gönczy P, Echeverri C, Oegema K, Coulson A, Jones SJ, Copley RR, Duperon J, Oegema J, Brehm M, Cassin E, et al. Functional genomic analysis of cell division in C. elegans using RNAi of genes on chromosome III. Nature. 2000 Nov 16;408(6810):331–336. [PubMed]
  • Piano F, Schetter AJ, Mangone M, Stein L, Kemphues KJ. RNAi analysis of genes expressed in the ovary of Caenorhabditis elegans. Curr Biol. 2000 Dec 14;10(24):1619–1622. [PubMed]
  • Grishok A, Tabara H, Mello CC. Genetic requirements for inheritance of RNAi in C. elegans. Science. 2000 Mar 31;287(5462):2494–2497. [PubMed]
  • Reinke V, Smith HE, Nance J, Wang J, Van Doren C, Begley R, Jones SJ, Davis EB, Scherer S, Ward S, et al. A global profile of germline gene expression in C. elegans. Mol Cell. 2000 Sep;6(3):605–616. [PubMed]
  • Grishok A, Pasquinelli AE, Conte D, Li N, Parrish S, Ha I, Baillie DL, Fire A, Ruvkun G, Mello CC. Genes and mechanisms related to RNA interference regulate expression of the small temporal RNAs that control C. elegans developmental timing. Cell. 2001 Jul 13;106(1):23–34. [PubMed]
  • Riggs CD. Meiotin-1: the meiosis readiness factor? Bioessays. 1997 Oct;19(10):925–931. [PubMed]
  • Roberts BT, Farr KA, Hoyt MA. The Saccharomyces cerevisiae checkpoint gene BUB1 encodes a novel protein kinase. Mol Cell Biol. 1994 Dec;14(12):8282–8291. [PMC free article] [PubMed]
  • Haber JE. The many interfaces of Mre11. Cell. 1998 Nov 25;95(5):583–586. [PubMed]
  • Hanazawa M, Mochii M, Ueno N, Kohara Y, Iino Y. Use of cDNA subtraction and RNA interference screens in combination reveals genes required for germ-line development in Caenorhabditis elegans. Proc Natl Acad Sci U S A. 2001 Jul 17;98(15):8686–8691. [PMC free article] [PubMed]
  • Scheel J, Srinivasan J, Honnert U, Henske A, Kurzchalia TV. Involvement of caveolin-1 in meiotic cell-cycle progression in Caenorhabditis elegans. Nat Cell Biol. 1999 Jun;1(2):127–129. [PubMed]
  • Hodgkin J, Horvitz HR, Brenner S. Nondisjunction Mutants of the Nematode CAENORHABDITIS ELEGANS. Genetics. 1979 Jan;91(1):67–94. [PMC free article] [PubMed]
  • Smardon A, Spoerke JM, Stacey SC, Klein ME, Mackin N, Maine EM. EGO-1 is related to RNA-directed RNA polymerase and functions in germ-line development and RNA interference in C. elegans. Curr Biol. 2000 Feb 24;10(4):169–178. [PubMed]
  • Howe M, McDonald KL, Albertson DG, Meyer BJ. HIM-10 is required for kinetochore structure and function on Caenorhabditis elegans holocentric chromosomes. J Cell Biol. 2001 Jun 11;153(6):1227–1238. [PMC free article] [PubMed]
  • Villeneuve AM. A cis-acting locus that promotes crossing over between X chromosomes in Caenorhabditis elegans. Genetics. 1994 Mar;136(3):887–902. [PMC free article] [PubMed]
  • Hubbard EJ, Greenstein D. The Caenorhabditis elegans gonad: a test tube for cell and developmental biology. Dev Dyn. 2000 May;218(1):2–22. [PubMed]
  • Villeneuve AM, Hillers KJ. Whence meiosis? Cell. 2001 Sep 21;106(6):647–650. [PubMed]
  • Jantsch-Plunger V, Gönczy P, Romano A, Schnabel H, Hamill D, Schnabel R, Hyman AA, Glotzer M. CYK-4: A Rho family gtpase activating protein (GAP) required for central spindle formation and cytokinesis. J Cell Biol. 2000 Jun 26;149(7):1391–1404. [PMC free article] [PubMed]
  • Wigge PA, Jensen ON, Holmes S, Souès S, Mann M, Kilmartin JV. Analysis of the Saccharomyces spindle pole by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. J Cell Biol. 1998 May 18;141(4):967–977. [PMC free article] [PubMed]
  • Kadyk LC, Kimble J. Genetic regulation of entry into meiosis in Caenorhabditis elegans. Development. 1998 May;125(10):1803–1813. [PubMed]
  • Zetka MC, Rose AM. Mutant rec-1 eliminates the meiotic pattern of crossing over in Caenorhabditis elegans. Genetics. 1995 Dec;141(4):1339–1349. [PMC free article] [PubMed]
  • Kelly KO, Dernburg AF, Stanfield GM, Villeneuve AM. Caenorhabditis elegans msh-5 is required for both normal and radiation-induced meiotic crossing over but not for completion of meiosis. Genetics. 2000 Oct;156(2):617–630. [PMC free article] [PubMed]
  • Zetka MC, Kawasaki I, Strome S, Müller F. Synapsis and chiasma formation in Caenorhabditis elegans require HIM-3, a meiotic chromosome core component that functions in chromosome segregation. Genes Dev. 1999 Sep 1;13(17):2258–2270. [PMC free article] [PubMed]
  • Ketting RF, Fischer SE, Bernstein E, Sijen T, Hannon GJ, Plasterk RH. Dicer functions in RNA interference and in synthesis of small RNA involved in developmental timing in C. elegans. Genes Dev. 2001 Oct 15;15(20):2654–2659. [PMC free article] [PubMed]
  • Zickler D, Kleckner N. Meiotic chromosomes: integrating structure and function. Annu Rev Genet. 1999;33:603–754. [PubMed]

Articles from Genetics are provided here courtesy of Genetics Society of America

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

  • Cited in Books
    Cited in Books
    PubMed Central articles cited in books
  • Gene
    Gene
    Gene links
  • GEO Profiles
    GEO Profiles
    Related GEO records
  • HomoloGene
    HomoloGene
    HomoloGene links
  • MedGen
    MedGen
    Related information in MedGen
  • Pathways + GO
    Pathways + GO
    Pathways, annotations and biological systems (BioSystems) that cite the current article.
  • Protein
    Protein
    Published protein sequences
  • PubMed
    PubMed
    PubMed citations for these articles
  • Substance
    Substance
    PubChem Substance links
  • Taxonomy
    Taxonomy
    Related taxonomy entry
  • Taxonomy Tree
    Taxonomy Tree

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...