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Genetics. May 2002; 161(1): 325–332.
PMCID: PMC1462090

Comparative analysis of quantitative trait loci controlling glucosinolates, myrosinase and insect resistance in Arabidopsis thaliana.

Abstract

Evolutionary interactions among insect herbivores and plant chemical defenses have generated systems where plant compounds have opposing fitness consequences for host plants, depending on attack by various insect herbivores. This interplay complicates understanding of fitness costs and benefits of plant chemical defenses. We are studying the role of the glucosinolate-myrosinase chemical defense system in protecting Arabidopsis thaliana from specialist and generalist insect herbivory. We used two Arabidopsis recombinant inbred populations in which we had previously mapped QTL controlling variation in the glucosinolate-myrosinase system. In this study we mapped QTL controlling resistance to specialist (Plutella xylostella) and generalist (Trichoplusia ni) herbivores. We identified a number of QTL that are specific to one herbivore or the other, as well as a single QTL that controls resistance to both insects. Comparison of QTL for herbivory, glucosinolates, and myrosinase showed that T. ni herbivory is strongly deterred by higher glucosinolate levels, faster breakdown rates, and specific chemical structures. In contrast, P. xylostella herbivory is uncorrelated with variation in the glucosinolate-myrosinase system. This agrees with evolutionary theory stating that specialist insects may overcome host plant chemical defenses, whereas generalists will be sensitive to these same defenses.

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Selected References

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  • Griffiths DW, Deighton N, Birch AN, Patrian B, Baur R, Städler E. Identification of glucosinolates on the leaf surface of plants from the Cruciferae and other closely related species. Phytochemistry. 2001 Jul;57(5):693–700. [PubMed]
  • Jander G, Cui J, Nhan B, Pierce NE, Ausubel FM. The TASTY locus on chromosome 1 of Arabidopsis affects feeding of the insect herbivore Trichoplusia ni. Plant Physiol. 2001 Jun;126(2):890–898. [PMC free article] [PubMed]
  • Kliebenstein DJ, Gershenzon J, Mitchell-Olds T. Comparative quantitative trait loci mapping of aliphatic, indolic and benzylic glucosinolate production in Arabidopsis thaliana leaves and seeds. Genetics. 2001 Sep;159(1):359–370. [PMC free article] [PubMed]
  • Albright SG, Lingley LH, Seeds JW, Lincoln-Boyea B. Pitfalls of gestational age reassignment in evaluation of low maternal serum alpha-fetoprotein levels. Am J Obstet Gynecol. 1988 Aug;159(2):369–370. [PubMed]
  • Alonso-Blanco C, El-Assal SE, Coupland G, Koornneef M. Analysis of natural allelic variation at flowering time loci in the Landsberg erecta and Cape Verde Islands ecotypes of Arabidopsis thaliana. Genetics. 1998 Jun;149(2):749–764. [PMC free article] [PubMed]
  • Kliebenstein DJ, Kroymann J, Brown P, Figuth A, Pedersen D, Gershenzon J, Mitchell-Olds T. Genetic control of natural variation in Arabidopsis glucosinolate accumulation. Plant Physiol. 2001 Jun;126(2):811–825. [PMC free article] [PubMed]
  • Kliebenstein DJ, Lambrix VM, Reichelt M, Gershenzon J, Mitchell-Olds T. Gene duplication in the diversification of secondary metabolism: tandem 2-oxoglutarate-dependent dioxygenases control glucosinolate biosynthesis in Arabidopsis. Plant Cell. 2001 Mar;13(3):681–693. [PMC free article] [PubMed]
  • Alonso-Blanco C, Peeters AJ, Koornneef M, Lister C, Dean C, van den Bosch N, Pot J, Kuiper MT. Development of an AFLP based linkage map of Ler, Col and Cvi Arabidopsis thaliana ecotypes and construction of a Ler/Cvi recombinant inbred line population. Plant J. 1998 Apr;14(2):259–271. [PubMed]
  • Lambrix V, Reichelt M, Mitchell-Olds T, Kliebenstein DJ, Gershenzon J. The Arabidopsis epithiospecifier protein promotes the hydrolysis of glucosinolates to nitriles and influences Trichoplusia ni herbivory. Plant Cell. 2001 Dec;13(12):2793–2807. [PMC free article] [PubMed]
  • Mitchell-Olds T, Pedersen D. The molecular basis of quantitative genetic variation in central and secondary metabolism in Arabidopsis. Genetics. 1998 Jun;149(2):739–747. [PMC free article] [PubMed]
  • Moyes CL, Raybould AF. The role of spatial scale and intraspecific variation in secondary chemistry in host-plant location by Ceutorhynchus assimilis (Coleoptera: Curculionidae). Proc Biol Sci. 2001 Aug 7;268(1476):1567–1573. [PMC free article] [PubMed]
  • Bradburne RP, Mithen R. Glucosinolate genetics and the attraction of the aphid parasitoid Diaeretiella rapae to Brassica. Proc Biol Sci. 2000 Jan 7;267(1438):89–95. [PMC free article] [PubMed]
  • Da Costa CP, Jones CM. Cucumber Beetle Resistance and Mite Susceptibility Controlled by the Bitter Gene in Cucumis sativus L. Science. 1971 Jun 11;172(3988):1145–1146. [PubMed]
  • Stotz HU, Pittendrigh BR, Kroymann J, Weniger K, Fritsche J, Bauke A, Mitchell-Olds T. Induced plant defense responses against chewing insects. Ethylene signaling reduces resistance of Arabidopsis against Egyptian cotton worm but not diamondback moth. Plant Physiol. 2000 Nov;124(3):1007–1018. [PMC free article] [PubMed]

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