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Genetics. Mar 2002; 160(3): 1035–1050.
PMCID: PMC1462010

A misexpression study examining dorsal thorax formation in Drosophila melanogaster.


We studied thorax formation in Drosophila melanogaster using a misexpression screen with EP lines and thoracic Gal4 drivers that provide a genetically sensitized background. We identified 191 interacting lines showing alterations of thoracic bristles (number and/or location), thorax and scutellum malformations, lethality, or suppression of the thoracic phenotype used in the screen. We analyzed these lines and showed that known genes with different functional roles (selector, prepattern, proneural, cell cycle regulation, lineage restriction, signaling pathways, transcriptional control, and chromatin organization) are among the modifier lines. A few lines have previously been identified in thorax formation, but others, such as chromatin-remodeling complex genes, are novel. However, most of the interacting loci are uncharacterized, providing a wealth of new genetic data. We also describe one such novel line, poco pelo (ppo), where both misexpression and loss-of-function phenotypes are similar: loss of bristles and scutellum malformation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Abdelilah-Seyfried S, Chan YM, Zeng C, Justice NJ, Younger-Shepherd S, Sharp LE, Barbel S, Meadows SA, Jan LY, Jan YN. A gain-of-function screen for genes that affect the development of the Drosophila adult external sensory organ. Genetics. 2000 Jun;155(2):733–752. [PMC free article] [PubMed]
  • Adams MD, Celniker SE, Holt RA, Evans CA, Gocayne JD, Amanatides PG, Scherer SE, Li PW, Hoskins RA, Galle RF, et al. The genome sequence of Drosophila melanogaster. Science. 2000 Mar 24;287(5461):2185–2195. [PubMed]
  • Agnès F, Suzanne M, Noselli S. The Drosophila JNK pathway controls the morphogenesis of imaginal discs during metamorphosis. Development. 1999 Dec;126(23):5453–5462. [PubMed]
  • Ashburner M, Thompson P, Roote J, Lasko PF, Grau Y, el Messal M, Roth S, Simpson P. The genetics of a small autosomal region of Drosophila melanogaster containing the structural gene for alcohol dehydrogenase. VII. Characterization of the region around the snail and cactus loci. Genetics. 1990 Nov;126(3):679–694. [PMC free article] [PubMed]
  • Blair SS. Compartments and appendage development in Drosophila. Bioessays. 1995 Apr;17(4):299–309. [PubMed]
  • Calleja M, Moreno E, Pelaz S, Morata G. Visualization of gene expression in living adult Drosophila. Science. 1996 Oct 11;274(5285):252–255. [PubMed]
  • Calleja M, Herranz H, Estella C, Casal J, Lawrence P, Simpson P, Morata G. Generation of medial and lateral dorsal body domains by the pannier gene of Drosophila. Development. 2000 Sep;127(18):3971–3980. [PubMed]
  • Campbell SD, Duttaroy A, Katzen AL, Chovnick A. Cloning and characterization of the scalloped region of Drosophila melanogaster. Genetics. 1991 Feb;127(2):367–380. [PMC free article] [PubMed]
  • Campbell S, Inamdar M, Rodrigues V, Raghavan V, Palazzolo M, Chovnick A. The scalloped gene encodes a novel, evolutionarily conserved transcription factor required for sensory organ differentiation in Drosophila. Genes Dev. 1992 Mar;6(3):367–379. [PubMed]
  • Campuzano S, Modolell J. Patterning of the Drosophila nervous system: the achaete-scute gene complex. Trends Genet. 1992 Jun;8(6):202–208. [PubMed]
  • Chen X, Fischer JA. In vivo Structure/Function analysis of the Drosophila fat facets deubiquitinating enzyme gene. Genetics. 2000 Dec;156(4):1829–1836. [PMC free article] [PubMed]
  • Chen Y, Struhl G. In vivo evidence that Patched and Smoothened constitute distinct binding and transducing components of a Hedgehog receptor complex. Development. 1998 Dec;125(24):4943–4948. [PubMed]
  • Lundgren SE, Callahan CA, Thor S, Thomas JB. Control of neuronal pathway selection by the Drosophila LIM homeodomain gene apterous. Development. 1995 Jun;121(6):1769–1773. [PubMed]
  • Chou TB, Perrimon N. The autosomal FLP-DFS technique for generating germline mosaics in Drosophila melanogaster. Genetics. 1996 Dec;144(4):1673–1679. [PMC free article] [PubMed]
  • Maniatis T. A ubiquitin ligase complex essential for the NF-kappaB, Wnt/Wingless, and Hedgehog signaling pathways. Genes Dev. 1999 Mar 1;13(5):505–510. [PubMed]
  • Clifford RJ, Schüpbach T. Coordinately and differentially mutable activities of torpedo, the Drosophila melanogaster homolog of the vertebrate EGF receptor gene. Genetics. 1989 Dec;123(4):771–787. [PMC free article] [PubMed]
  • Martin-Blanco E, Pastor-Pareja JC, Garcia-Bellido A. JNK and decapentaplegic signaling control adhesiveness and cytoskeleton dynamics during thorax closure in Drosophila. Proc Natl Acad Sci U S A. 2000 Jul 5;97(14):7888–7893. [PMC free article] [PubMed]
  • Cohen B, McGuffin ME, Pfeifle C, Segal D, Cohen SM. apterous, a gene required for imaginal disc development in Drosophila encodes a member of the LIM family of developmental regulatory proteins. Genes Dev. 1992 May;6(5):715–729. [PubMed]
  • Daubresse G, Deuring R, Moore L, Papoulas O, Zakrajsek I, Waldrip WR, Scott MP, Kennison JA, Tamkun JW. The Drosophila kismet gene is related to chromatin-remodeling factors and is required for both segmentation and segment identity. Development. 1999 Mar;126(6):1175–1187. [PubMed]
  • Milán M, Campuzano S, García-Bellido A. Developmental parameters of cell death in the wing disc of Drosophila. Proc Natl Acad Sci U S A. 1997 May 27;94(11):5691–5696. [PMC free article] [PubMed]
  • Edgar BA, Lehman DA, O'Farrell PH. Transcriptional regulation of string (cdc25): a link between developmental programming and the cell cycle. Development. 1994 Nov;120(11):3131–3143. [PMC free article] [PubMed]
  • Ellis HM, Spann DR, Posakony JW. extramacrochaetae, a negative regulator of sensory organ development in Drosophila, defines a new class of helix-loop-helix proteins. Cell. 1990 Apr 6;61(1):27–38. [PubMed]
  • Mosrin-Huaman C, Canaple L, Locker D, Decoville M. DSP1 gene of Drosophila melanogaster encodes an HMG-domain protein that plays multiple roles in development. Dev Genet. 1998;23(4):324–334. [PubMed]
  • Fernandez-Funez P, Nino-Rosales ML, de Gouyon B, She WC, Luchak JM, Martinez P, Turiegano E, Benito J, Capovilla M, Skinner PJ, et al. Identification of genes that modify ataxin-1-induced neurodegeneration. Nature. 2000 Nov 2;408(6808):101–106. [PubMed]
  • Nellen D, Burke R, Struhl G, Basler K. Direct and long-range action of a DPP morphogen gradient. Cell. 1996 May 3;85(3):357–368. [PubMed]
  • Nüsslein-Volhard C, Wieschaus E. Mutations affecting segment number and polarity in Drosophila. Nature. 1980 Oct 30;287(5785):795–801. [PubMed]
  • Olivier JP, Raabe T, Henkemeyer M, Dickson B, Mbamalu G, Margolis B, Schlessinger J, Hafen E, Pawson T. A Drosophila SH2-SH3 adaptor protein implicated in coupling the sevenless tyrosine kinase to an activator of Ras guanine nucleotide exchange, Sos. Cell. 1993 Apr 9;73(1):179–191. [PubMed]
  • Gomez-Skarmeta JL, Diez del Corral R, de la Calle-Mustienes E, Ferré-Marcó D, Modolell J. Araucan and caupolican, two members of the novel iroquois complex, encode homeoproteins that control proneural and vein-forming genes. Cell. 1996 Apr 5;85(1):95–105. [PubMed]
  • Papoulas O, Beek SJ, Moseley SL, McCallum CM, Sarte M, Shearn A, Tamkun JW. The Drosophila trithorax group proteins BRM, ASH1 and ASH2 are subunits of distinct protein complexes. Development. 1998 Oct;125(20):3955–3966. [PubMed]
  • Haenlin M, Cubadda Y, Blondeau F, Heitzler P, Lutz Y, Simpson P, Ramain P. Transcriptional activity of pannier is regulated negatively by heterodimerization of the GATA DNA-binding domain with a cofactor encoded by the u-shaped gene of Drosophila. Genes Dev. 1997 Nov 15;11(22):3096–3108. [PMC free article] [PubMed]
  • Pecasse F, Beck Y, Ruiz C, Richards G. Krüppel-homolog, a stage-specific modulator of the prepupal ecdysone response, is essential for Drosophila metamorphosis. Dev Biol. 2000 May 1;221(1):53–67. [PubMed]
  • Halder G, Polaczyk P, Kraus ME, Hudson A, Kim J, Laughon A, Carroll S. The Vestigial and Scalloped proteins act together to directly regulate wing-specific gene expression in Drosophila. Genes Dev. 1998 Dec 15;12(24):3900–3909. [PMC free article] [PubMed]
  • Phillips RG, Warner NL, Whittle JR. Wingless signaling leads to an asymmetric response to decapentaplegic-dependent signaling during sense organ patterning on the notum of Drosophila melanogaster. Dev Biol. 1999 Mar 1;207(1):150–162. [PubMed]
  • Hayashi S, Hirose S, Metcalfe T, Shirras AD. Control of imaginal cell development by the escargot gene of Drosophila. Development. 1993 May;118(1):105–115. [PubMed]
  • Posakony JW. Nature versus nurture: asymmetric cell divisions in Drosophila bristle development. Cell. 1994 Feb 11;76(3):415–418. [PubMed]
  • Heitzler P, Haenlin M, Ramain P, Calleja M, Simpson P. A genetic analysis of pannier, a gene necessary for viability of dorsal tissues and bristle positioning in Drosophila. Genetics. 1996 Jul;143(3):1271–1286. [PMC free article] [PubMed]
  • Huang AM, Rubin GM. A misexpression screen identifies genes that can modulate RAS1 pathway signaling in Drosophila melanogaster. Genetics. 2000 Nov;156(3):1219–1230. [PMC free article] [PubMed]
  • Riesgo-Escovar JR, Hafen E. Common and distinct roles of DFos and DJun during Drosophila development. Science. 1997 Oct 24;278(5338):669–672. [PubMed]
  • Ito T, Bulger M, Pazin MJ, Kobayashi R, Kadonaga JT. ACF, an ISWI-containing and ATP-utilizing chromatin assembly and remodeling factor. Cell. 1997 Jul 11;90(1):145–155. [PubMed]
  • Rørth P. A modular misexpression screen in Drosophila detecting tissue-specific phenotypes. Proc Natl Acad Sci U S A. 1996 Oct 29;93(22):12418–12422. [PMC free article] [PubMed]
  • Kennison JA, Tamkun JW. Dosage-dependent modifiers of polycomb and antennapedia mutations in Drosophila. Proc Natl Acad Sci U S A. 1988 Nov;85(21):8136–8140. [PMC free article] [PubMed]
  • Rørth P, Szabo K, Bailey A, Laverty T, Rehm J, Rubin GM, Weigmann K, Milán M, Benes V, Ansorge W, et al. Systematic gain-of-function genetics in Drosophila. Development. 1998 Mar;125(6):1049–1057. [PubMed]
  • Sato M, Kojima T, Michiue T, Saigo K. Bar homeobox genes are latitudinal prepattern genes in the developing Drosophila notum whose expression is regulated by the concerted functions of decapentaplegic and wingless. Development. 1999 Apr;126(7):1457–1466. [PubMed]
  • Kingston RE, Narlikar GJ. ATP-dependent remodeling and acetylation as regulators of chromatin fluidity. Genes Dev. 1999 Sep 15;13(18):2339–2352. [PubMed]
  • Simon MA, Bowtell DD, Dodson GS, Laverty TR, Rubin GM. Ras1 and a putative guanine nucleotide exchange factor perform crucial steps in signaling by the sevenless protein tyrosine kinase. Cell. 1991 Nov 15;67(4):701–716. [PubMed]
  • Lehming N, Le Saux A, Schüller J, Ptashne M. Chromatin components as part of a putative transcriptional repressing complex. Proc Natl Acad Sci U S A. 1998 Jun 23;95(13):7322–7326. [PMC free article] [PubMed]
  • Tomoyasu Y, Nakamura M, Ueno N. Role of dpp signalling in prepattern formation of the dorsocentral mechanosensory organ in Drosophila melanogaster. Development. 1998 Nov;125(21):4215–4224. [PubMed]
  • Winick J, Abel T, Leonard MW, Michelson AM, Chardon-Loriaux I, Holmgren RA, Maniatis T, Engel JD. A GATA family transcription factor is expressed along the embryonic dorsoventral axis in Drosophila melanogaster. Development. 1993 Dec;119(4):1055–1065. [PubMed]
  • Varga-Weisz PD, Wilm M, Bonte E, Dumas K, Mann M, Becker PB. Chromatin-remodelling factor CHRAC contains the ATPases ISWI and topoisomerase II. Nature. 1997 Aug 7;388(6642):598–602. [PubMed]
  • Zeitlinger J, Bohmann D. Thorax closure in Drosophila: involvement of Fos and the JNK pathway. Development. 1999 Sep;126(17):3947–3956. [PubMed]
  • Verheyen EM, Purcell KJ, Fortini ME, Artavanis-Tsakonas S. Analysis of dominant enhancers and suppressors of activated Notch in Drosophila. Genetics. 1996 Nov;144(3):1127–1141. [PMC free article] [PubMed]

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