Logo of geneticsGeneticsCurrent IssueInformation for AuthorsEditorial BoardSubscribeSubmit a Manuscript
Genetics. Oct 2001; 159(2): 823–837.
PMCID: PMC1461827

Transmission genetics of chromatin from a synthetic amphidiploid to cultivated peanut (Arachis hypogaea L.). broadening the gene pool of a monophyletic polyploid species.


Polyploidy creates severe genetic bottlenecks, contributing to the genetic vulnerability of leading crops. Cultivated peanut is thought to be of monophyletic origin, harboring relatively little genetic diversity. To introduce variability from diploid wild species into tetraploid cultivated Arachis hypogaea, a synthetic amphidiploid [[A. batizocoi K9484 x (A. cardenasii GKP10017 x A. diogoi GKP10602)](4x)] was used as donor parent to generate a backcross population of 78 progeny. Three hundred seventy RFLP loci were mapped onto 23 linkage groups, spanning 2210 cM. Chromatin derived from the two A-genome diploid ancestors (A. cardenasii and A. diogoi) comprised mosaic chromosomes, reflecting crossing over in the diploid A-genome interspecific F(1) hybrid. Recombination between chromosomes in the tetraploid progeny was similar to chromosome pairing reported for A. hypogaea, with recombination generally between chromosomes of the same subgenomic affinity. Segregation distortion was observed for 25% of the markers, distributed over 20 linkage groups. Unexpectedly, 68% of the markers deviating from expected segregation showed an excess of the synthetic parent allele. Genetic consequences, relationship to species origins, and significance for comparative genetics are discussed.

Full Text

The Full Text of this article is available as a PDF (935K).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Chen ZJ, Comai L, Pikaard CS. Gene dosage and stochastic effects determine the severity and direction of uniparental ribosomal RNA gene silencing (nucleolar dominance) in Arabidopsis allopolyploids. Proc Natl Acad Sci U S A. 1998 Dec 8;95(25):14891–14896. [PMC free article] [PubMed]
  • Eshed Y, Zamir D. An introgression line population of Lycopersicon pennellii in the cultivated tomato enables the identification and fine mapping of yield-associated QTL. Genetics. 1995 Nov;141(3):1147–1162. [PMC free article] [PubMed]
  • Feldman M, Liu B, Segal G, Abbo S, Levy AA, Vega JM. Rapid elimination of low-copy DNA sequences in polyploid wheat: a possible mechanism for differentiation of homoeologous chromosomes. Genetics. 1997 Nov;147(3):1381–1387. [PMC free article] [PubMed]
  • Furner IJ, Sheikh MA, Collett CE. Gene silencing and homology-dependent gene silencing in Arabidopsis: genetic modifiers and DNA methylation. Genetics. 1998 Jun;149(2):651–662. [PMC free article] [PubMed]
  • Garcia GM, Stalker HT, Kochert G. Introgression analysis of an interspecific hybrid population in peanuts (Arachis hypogaea L.) using RFLP and RAPD markers. Genome. 1995 Feb;38(1):166–176. [PubMed]
  • Gaut BS, Doebley JF. DNA sequence evidence for the segmental allotetraploid origin of maize. Proc Natl Acad Sci U S A. 1997 Jun 24;94(13):6809–6814. [PMC free article] [PubMed]
  • Gaut BS, Le Thierry d'Ennequin M, Peek AS, Sawkins MC. Maize as a model for the evolution of plant nuclear genomes. Proc Natl Acad Sci U S A. 2000 Jun 20;97(13):7008–7015. [PMC free article] [PubMed]
  • Halward T, Stalker T, LaRue E, Kochert G. Use of single-primer DNA amplifications in genetic studies of peanut (Arachis hypogaea L.). Plant Mol Biol. 1992 Jan;18(2):315–325. [PubMed]
  • Jiang C, Wright RJ, El-Zik KM, Paterson AH. Polyploid formation created unique avenues for response to selection in Gossypium (cotton). Proc Natl Acad Sci U S A. 1998 Apr 14;95(8):4419–4424. [PMC free article] [PubMed]
  • Jiang CX, Chee PW, Draye X, Morrell PL, Smith CW, Paterson AH. Multilocus interactions restrict gene introgression in interspecific populations of polyploid Gossypium (cotton). Evolution. 2000 Jun;54(3):798–814. [PubMed]
  • Kowalski SP, Lan TH, Feldmann KA, Paterson AH. Comparative mapping of Arabidopsis thaliana and Brassica oleracea chromosomes reveals islands of conserved organization. Genetics. 1994 Oct;138(2):499–510. [PMC free article] [PubMed]
  • Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newberg LA, Newburg L. MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics. 1987 Oct;1(2):174–181. [PubMed]
  • Masterson J. Stomatal size in fossil plants: evidence for polyploidy in majority of angiosperms. Science. 1994 Apr 15;264(5157):421–424. [PubMed]
  • Paterson AH, Lan TH, Reischmann KP, Chang C, Lin YR, Liu SC, Burow MD, Kowalski SP, Katsar CS, DelMonte TA, et al. Toward a unified genetic map of higher plants, transcending the monocot-dicot divergence. Nat Genet. 1996 Dec;14(4):380–382. [PubMed]
  • Soltis DE, Soltis PS. The dynamic nature of polyploid genomes. Proc Natl Acad Sci U S A. 1995 Aug 29;92(18):8089–8091. [PMC free article] [PubMed]
  • Paterson AH, Bowers JE, Burow MD, Draye X, Elsik CG, Jiang CX, Katsar CS, Lan TH, Lin YR, Ming R, et al. Comparative genomics of plant chromosomes. Plant Cell. 2000 Sep;12(9):1523–1540. [PMC free article] [PubMed]
  • Song K, Lu P, Tang K, Osborn TC. Rapid genome change in synthetic polyploids of Brassica and its implications for polyploid evolution. Proc Natl Acad Sci U S A. 1995 Aug 15;92(17):7719–7723. [PMC free article] [PubMed]
  • Reinisch AJ, Dong JM, Brubaker CL, Stelly DM, Wendel JF, Paterson AH. A detailed RFLP map of cotton, Gossypium hirsutum x Gossypium barbadense: chromosome organization and evolution in a disomic polyploid genome. Genetics. 1994 Nov;138(3):829–847. [PMC free article] [PubMed]
  • Sears ER. Genetic control of chromosome pairing in wheat. Annu Rev Genet. 1976;10:31–51. [PubMed]

Articles from Genetics are provided here courtesy of Genetics Society of America


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...