• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of geneticsGeneticsCurrent IssueInformation for AuthorsEditorial BoardSubscribeSubmit a Manuscript
Genetics. Jul 2001; 158(3): 1217–1226.
PMCID: PMC1461731

Quantitative trait locus mapping in laboratory mice derived from a replicated selection experiment for open-field activity.

Abstract

Bidirectional selection in rodents has been used to derive animal models of human behavior. An important question is whether selection for behavior operates on a limited number of QTL or whether the number and individual contribution of QTL varies between selection experiments. To address this question, we mapped QTL in two large F2 intercrosses (N = 815 and 821) from the four lines derived from a replicated selection experiment for open-field activity, an animal model for susceptibility to anxiety. Our analyses indicate that selection operated on the same relatively small number of loci in both crosses. Haplotype information and the direction of effect of each QTL allele were used to confirm that the QTL mapped in the two crosses lie in the same chromosomal regions, although we were unable to determine whether QTL in the two crosses represent the same genes. We conclude that the genetic architecture of the selected strains is similar and relatively simple.

Full Text

The Full Text of this article is available as a PDF (105K).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • van Abeelen JH, Kroes HW. Albinism and mouse behaviour. Genetica. 1968;38(4):419–429. [PubMed]
  • Churchill GA, Doerge RW. Empirical threshold values for quantitative trait mapping. Genetics. 1994 Nov;138(3):963–971. [PMC free article] [PubMed]
  • Crabbe JC, Wahlsten D, Dudek BC. Genetics of mouse behavior: interactions with laboratory environment. Science. 1999 Jun 4;284(5420):1670–1672. [PubMed]
  • Darvasi A. Experimental strategies for the genetic dissection of complex traits in animal models. Nat Genet. 1998 Jan;18(1):19–24. [PubMed]
  • Darvasi A, Soller M. A simple method to calculate resolving power and confidence interval of QTL map location. Behav Genet. 1997 Mar;27(2):125–132. [PubMed]
  • DeFries JC. Pleiotropic effects of albinism on open field behaviour in mice. Nature. 1969 Jan 4;221(5175):65–66. [PubMed]
  • DeFries JC, Gervais MC, Thomas EA. Response to 30 generations of selection for open-field activity in laboratory mice. Behav Genet. 1978 Jan;8(1):3–13. [PubMed]
  • Doerge RW, Churchill GA. Permutation tests for multiple loci affecting a quantitative character. Genetics. 1996 Jan;142(1):285–294. [PMC free article] [PubMed]
  • Driscoll P, Escorihuela RM, Fernández-Teruel A, Giorgi O, Schwegler H, Steimer T, Wiersma A, Corda MG, Flint J, Koolhaas JM, et al. Genetic selection and differential stress responses. The Roman lines/strains of rats. Ann N Y Acad Sci. 1998 Jun 30;851:501–510. [PubMed]
  • Ferré P, Fernández-Teruel A, Escorihuela RM, Driscoll P, Corda MG, Giorgi O, Tobeña A. Behavior of the Roman/Verh high- and low-avoidance rat lines in anxiety tests: relationship with defecation and self-grooming. Physiol Behav. 1995 Dec;58(6):1209–1213. [PubMed]
  • Flint J, Corley R, DeFries JC, Fulker DW, Gray JA, Miller S, Collins AC. A simple genetic basis for a complex psychological trait in laboratory mice. Science. 1995 Sep 8;269(5229):1432–1435. [PubMed]
  • Jiang C, Zeng ZB. Multiple trait analysis of genetic mapping for quantitative trait loci. Genetics. 1995 Jul;140(3):1111–1127. [PMC free article] [PubMed]
  • Katz RJ, Doyle RL. The albino locus and locomotor behavior in the mouse: studies using extended test intervals. Behav Genet. 1981 Mar;11(2):167–172. [PubMed]
  • Kwon BS, Haq AK, Wakulchik M, Kestler D, Barton DE, Francke U, Lamoreux ML, Whitney JB, 3rd, Halaban R. Isolation, chromosomal mapping, and expression of the mouse tyrosinase gene. J Invest Dermatol. 1989 Nov;93(5):589–594. [PubMed]
  • Risch N, Botstein D. A manic depressive history. Nat Genet. 1996 Apr;12(4):351–353. [PubMed]
  • Lander ES, Botstein D. Mapping mendelian factors underlying quantitative traits using RFLP linkage maps. Genetics. 1989 Jan;121(1):185–199. [PMC free article] [PubMed]
  • Risch N, Merikangas K. The future of genetic studies of complex human diseases. Science. 1996 Sep 13;273(5281):1516–1517. [PubMed]
  • Thiessen DD, Lindzey G, Owen K. Behavior and allelic variations in enzyme activity and coat color at the C locus of the mouse. Behav Genet. 1970 Aug-Nov;1(3):257–267. [PubMed]
  • Turri MG, Talbot CJ, Radcliffe RA, Wehner JM, Flint J. High-resolution mapping of quantitative trait loci for emotionality in selected strains of mice. Mamm Genome. 1999 Nov;10(11):1098–1101. [PubMed]
  • Liebsch G, Linthorst AC, Neumann ID, Reul JM, Holsboer F, Landgraf R. Behavioral, physiological, and neuroendocrine stress responses and differential sensitivity to diazepam in two Wistar rat lines selectively bred for high- and low-anxiety-related behavior. Neuropsychopharmacology. 1998 Nov;19(5):381–396. [PubMed]
  • Uhl GR, Gold LH, Risch N. Genetic analyses of complex behavioral disorders. Proc Natl Acad Sci U S A. 1997 Apr 1;94(7):2785–2786. [PMC free article] [PubMed]
  • Liebsch G, Montkowski A, Holsboer F, Landgraf R. Behavioural profiles of two Wistar rat lines selectively bred for high or low anxiety-related behaviour. Behav Brain Res. 1998 Aug;94(2):301–310. [PubMed]
  • Van Etten WJ, Steen RG, Nguyen H, Castle AB, Slonim DK, Ge B, Nusbaum C, Schuler GD, Lander ES, Hudson TJ. Radiation hybrid map of the mouse genome. Nat Genet. 1999 Aug;22(4):384–387. [PubMed]
  • McCarthy LC, Bihoreau MT, Kiguwa SL, Browne J, Watanabe TK, Hishigaki H, Tsuji A, Kiel S, Webber C, Davis ME, et al. A whole-genome radiation hybrid panel and framework map of the rat genome. Mamm Genome. 2000 Sep;11(9):791–795. [PubMed]
  • Watanabe TK, Bihoreau MT, McCarthy LC, Kiguwa SL, Hishigaki H, Tsuji A, Browne J, Yamasaki Y, Mizoguchi-Miyakita A, Oga K, et al. A radiation hybrid map of the rat genome containing 5,255 markers. Nat Genet. 1999 May;22(1):27–36. [PubMed]
  • Melchinger AE, Utz HF, Schön CC. Quantitative trait locus (QTL) mapping using different testers and independent population samples in maize reveals low power of QTL detection and large bias in estimates of QTL effects. Genetics. 1998 May;149(1):383–403. [PMC free article] [PubMed]
  • Zeng ZB. Precision mapping of quantitative trait loci. Genetics. 1994 Apr;136(4):1457–1468. [PMC free article] [PubMed]

Articles from Genetics are provided here courtesy of Genetics Society of America

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...