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Genetics. Apr 2001; 157(4): 1469–1480.
PMCID: PMC1461599

Isolation and characterization of WHI3, a size-control gene of Saccharomyces cerevisiae.

Abstract

WHI3 is a gene affecting size control and cell cycle in the yeast Saccharomyces cerevisiae. The whi3 mutant has small cells, while extra doses of WHI3 produce large cells, and a large excess of WHI3 produces a lethal arrest in G1 phase. WHI3 seems to be a dose-dependent inhibitor of Start. Whi3 and its partially redundant homolog Whi4 have an RNA-binding domain, and mutagenesis experiments indicate that this RNA-binding domain is essential for Whi3 function. CLN3-1 whi3 cells are extremely small, nearly sterile, and largely nonresponsive to mating factor. Fertility is restored by deletion of CLN2, suggesting that whi3 cells may have abnormally high levels of CLN2 function.

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Selected References

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  • Dirick L, Böhm T, Nasmyth K. Roles and regulation of Cln-Cdc28 kinases at the start of the cell cycle of Saccharomyces cerevisiae. EMBO J. 1995 Oct 2;14(19):4803–4813. [PMC free article] [PubMed]
  • Garfinkel DJ, Strathern JN. Ty mutagenesis in Saccharomyces cerevisiae. Methods Enzymol. 1991;194:342–361. [PubMed]
  • Garfinkel DJ, Mastrangelo MF, Sanders NJ, Shafer BK, Strathern JN. Transposon tagging using Ty elements in yeast. Genetics. 1988 Sep;120(1):95–108. [PMC free article] [PubMed]
  • Gartner A, Jovanović A, Jeoung DI, Bourlat S, Cross FR, Ammerer G. Pheromone-dependent G1 cell cycle arrest requires Far1 phosphorylation, but may not involve inhibition of Cdc28-Cln2 kinase, in vivo. Mol Cell Biol. 1998 Jul;18(7):3681–3691. [PMC free article] [PubMed]
  • Hartwell LH, Culotti J, Pringle JR, Reid BJ. Genetic control of the cell division cycle in yeast. Science. 1974 Jan 11;183(4120):46–51. [PubMed]
  • Johnston GC, Pringle JR, Hartwell LH. Coordination of growth with cell division in the yeast Saccharomyces cerevisiae. Exp Cell Res. 1977 Mar 1;105(1):79–98. [PubMed]
  • Ko HA, Moore SA. Kinetic characterization of a prestart cell division control step in yeast. Implications for the mechanism of alpha-factor-induced division arrest. J Biol Chem. 1990 Dec 15;265(35):21652–21663. [PubMed]
  • Koch C, Nasmyth K. Cell cycle regulated transcription in yeast. Curr Opin Cell Biol. 1994 Jun;6(3):451–459. [PubMed]
  • Loeb JD, Kerentseva TA, Pan T, Sepulveda-Becerra M, Liu H. Saccharomyces cerevisiae G1 cyclins are differentially involved in invasive and pseudohyphal growth independent of the filamentation mitogen-activated protein kinase pathway. Genetics. 1999 Dec;153(4):1535–1546. [PMC free article] [PubMed]
  • Mitchison JM, Nurse P. Growth in cell length in the fission yeast Schizosaccharomyces pombe. J Cell Sci. 1985 Apr;75:357–376. [PubMed]
  • Moore SA. Kinetic evidence for a critical rate of protein synthesis in the Saccharomyces cerevisiae yeast cell cycle. J Biol Chem. 1988 Jul 15;263(20):9674–9681. [PubMed]
  • Mösch HU, Fink GR. Dissection of filamentous growth by transposon mutagenesis in Saccharomyces cerevisiae. Genetics. 1997 Mar;145(3):671–684. [PMC free article] [PubMed]
  • Nash R, Tokiwa G, Anand S, Erickson K, Futcher AB. The WHI1+ gene of Saccharomyces cerevisiae tethers cell division to cell size and is a cyclin homolog. EMBO J. 1988 Dec 20;7(13):4335–4346. [PMC free article] [PubMed]
  • Neufeld TP, Edgar BA. Connections between growth and the cell cycle. Curr Opin Cell Biol. 1998 Dec;10(6):784–790. [PubMed]
  • Oehlen LJ, Cross FR. G1 cyclins CLN1 and CLN2 repress the mating factor response pathway at Start in the yeast cell cycle. Genes Dev. 1994 May 1;8(9):1058–1070. [PubMed]
  • Pardee AB. G1 events and regulation of cell proliferation. Science. 1989 Nov 3;246(4930):603–608. [PubMed]
  • Peterson EA, Evans WH. Separation of bone marrow cells by sedimentation at unit gravity. Nature. 1967 May 20;214(5090):824–825. [PubMed]
  • Abe H, Shimoda C. Autoregulated expression of Schizosaccharomyces pombe meiosis-specific transcription factor Mei4 and a genome-wide search for its target genes. Genetics. 2000 Apr;154(4):1497–1508. [PMC free article] [PubMed]
  • Polymenis M, Schmidt EV. Coupling of cell division to cell growth by translational control of the G1 cyclin CLN3 in yeast. Genes Dev. 1997 Oct 1;11(19):2522–2531. [PMC free article] [PubMed]
  • Birney E, Kumar S, Krainer AR. Analysis of the RNA-recognition motif and RS and RGG domains: conservation in metazoan pre-mRNA splicing factors. Nucleic Acids Res. 1993 Dec 25;21(25):5803–5816. [PMC free article] [PubMed]
  • Richardson HE, Wittenberg C, Cross F, Reed SI. An essential G1 function for cyclin-like proteins in yeast. Cell. 1989 Dec 22;59(6):1127–1133. [PubMed]
  • Schneider BL, Patton EE, Lanker S, Mendenhall MD, Wittenberg C, Futcher B, Tyers M. Yeast G1 cyclins are unstable in G1 phase. Nature. 1998 Sep 3;395(6697):86–89. [PubMed]
  • Carter BL, Sudbery PE. Small-sized mutants of Saccharomyces cerevisiae. Genetics. 1980 Nov;96(3):561–566. [PMC free article] [PubMed]
  • Chu S, DeRisi J, Eisen M, Mulholland J, Botstein D, Brown PO, Herskowitz I. The transcriptional program of sporulation in budding yeast. Science. 1998 Oct 23;282(5389):699–705. [PubMed]
  • Spellman PT, Sherlock G, Zhang MQ, Iyer VR, Anders K, Eisen MB, Brown PO, Botstein D, Futcher B. Comprehensive identification of cell cycle-regulated genes of the yeast Saccharomyces cerevisiae by microarray hybridization. Mol Biol Cell. 1998 Dec;9(12):3273–3297. [PMC free article] [PubMed]
  • Costanzo MC, Hogan JD, Cusick ME, Davis BP, Fancher AM, Hodges PE, Kondu P, Lengieza C, Lew-Smith JE, Lingner C, et al. The yeast proteome database (YPD) and Caenorhabditis elegans proteome database (WormPD): comprehensive resources for the organization and comparison of model organism protein information. Nucleic Acids Res. 2000 Jan 1;28(1):73–76. [PMC free article] [PubMed]
  • Stuart D, Wittenberg C. CLN3, not positive feedback, determines the timing of CLN2 transcription in cycling cells. Genes Dev. 1995 Nov 15;9(22):2780–2794. [PubMed]
  • Sudbery PE, Goodey AR, Carter BL. Genes which control cell proliferation in the yeast Saccharomyces cerevisiae. Nature. 1980 Nov 27;288(5789):401–404. [PubMed]
  • Sugimoto K, Matsumoto K, Kornberg RD, Reed SI, Wittenberg C. Dosage suppressors of the dominant G1 cyclin mutant CLN3-2: identification of a yeast gene encoding a putative RNA/ssDNA binding protein. Mol Gen Genet. 1995 Oct 25;248(6):712–718. [PubMed]
  • Tyers M, Tokiwa G, Futcher B. Comparison of the Saccharomyces cerevisiae G1 cyclins: Cln3 may be an upstream activator of Cln1, Cln2 and other cyclins. EMBO J. 1993 May;12(5):1955–1968. [PMC free article] [PubMed]
  • Thomas BJ, Rothstein R. Elevated recombination rates in transcriptionally active DNA. Cell. 1989 Feb 24;56(4):619–630. [PubMed]
  • Zetterberg A, Larsson O, Wiman KG. What is the restriction point? Curr Opin Cell Biol. 1995 Dec;7(6):835–842. [PubMed]
  • Tokiwa G, Tyers M, Volpe T, Futcher B. Inhibition of G1 cyclin activity by the Ras/cAMP pathway in yeast. Nature. 1994 Sep 22;371(6495):342–345. [PubMed]

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