• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of geneticsGeneticsCurrent IssueInformation for AuthorsEditorial BoardSubscribeSubmit a Manuscript
Genetics. Mar 2001; 157(3): 1169–1177.
PMCID: PMC1461566

Regulatory mechanisms controlling expression of the DAN/TIR mannoprotein genes during anaerobic remodeling of the cell wall in Saccharomyces cerevisiae.

Abstract

The DAN/TIR genes of Saccharomyces cerevisiae encode homologous mannoproteins, some of which are essential for anaerobic growth. Expression of these genes is induced during anaerobiosis and in some cases during cold shock. We show that several heme-responsive mechanisms combine to regulate DAN/TIR gene expression. The first mechanism employs two repression factors, Mox1 and Mox2, and an activation factor, Mox4 (for mannoprotein regulation by oxygen). The genes encoding these proteins were identified by selecting for recessive mutants with altered regulation of a dan1::ura3 fusion. MOX4 is identical to UPC2, encoding a binucleate zinc cluster protein controlling expression of an anaerobic sterol transport system. Mox4/Upc2 is required for expression of all the DAN/TIR genes. It appears to act through a consensus sequence termed the AR1 site, as does Mox2. The noninducible mox4Delta allele was epistatic to the constitutive mox1 and mox2 mutations, suggesting that Mox1 and Mox2 modulate activation by Mox4 in a heme-dependent fashion. Mutations in a putative repression domain in Mox4 caused constitutive expression of the DAN/TIR genes, indicating a role for this domain in heme repression. MOX4 expression is induced both in anaerobic and cold-shocked cells, so heme may also regulate DAN/TIR expression through inhibition of expression of MOX4. Indeed, ectopic expression of MOX4 in aerobic cells resulted in partially constitutive expression of DAN1. Heme also regulates expression of some of the DAN/TIR genes through the Rox7 repressor, which also controls expression of the hypoxic gene ANB1. In addition Rox1, another heme-responsive repressor, and the global repressors Tup1 and Ssn6 are also required for full aerobic repression of these genes.

Full Text

The Full Text of this article is available as a PDF (391K).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Keng T. HAP1 and ROX1 form a regulatory pathway in the repression of HEM13 transcription in Saccharomyces cerevisiae. Mol Cell Biol. 1992 Jun;12(6):2616–2623. [PMC free article] [PubMed]
  • Kondo K, Inouye M. TIP 1, a cold shock-inducible gene of Saccharomyces cerevisiae. J Biol Chem. 1991 Sep 15;266(26):17537–17544. [PubMed]
  • Kowalski LR, Kondo K, Inouye M. Cold-shock induction of a family of TIP1-related proteins associated with the membrane in Saccharomyces cerevisiae. Mol Microbiol. 1995 Jan;15(2):341–353. [PubMed]
  • Kwast KE, Burke PV, Staahl BT, Poyton RO. Oxygen sensing in yeast: evidence for the involvement of the respiratory chain in regulating the transcription of a subset of hypoxic genes. Proc Natl Acad Sci U S A. 1999 May 11;96(10):5446–5451. [PMC free article] [PubMed]
  • Lewis TL, Keesler GA, Fenner GP, Parks LW. Pleiotropic mutations in Saccharomyces cerevisiae affecting sterol uptake and metabolism. Yeast. 1988 Jun;4(2):93–106. [PubMed]
  • Lowry CV, Lieber RH. Negative regulation of the Saccharomyces cerevisiae ANB1 gene by heme, as mediated by the ROX1 gene product. Mol Cell Biol. 1986 Dec;6(12):4145–4148. [PMC free article] [PubMed]
  • Lowry CV, Zitomer RS. Oxygen regulation of anaerobic and aerobic genes mediated by a common factor in yeast. Proc Natl Acad Sci U S A. 1984 Oct;81(19):6129–6133. [PMC free article] [PubMed]
  • Lowry CV, Zitomer RS. ROX1 encodes a heme-induced repression factor regulating ANB1 and CYC7 of Saccharomyces cerevisiae. Mol Cell Biol. 1988 Nov;8(11):4651–4658. [PMC free article] [PubMed]
  • Lowry CV, Weiss JL, Walthall DA, Zitomer RS. Modulator sequences mediate oxygen regulation of CYC1 and a neighboring gene in yeast. Proc Natl Acad Sci U S A. 1983 Jan;80(1):151–155. [PMC free article] [PubMed]
  • Lowry CV, Cerdán ME, Zitomer RS. A hypoxic consensus operator and a constitutive activation region regulate the ANB1 gene of Saccharomyces cerevisiae. Mol Cell Biol. 1990 Nov;10(11):5921–5926. [PMC free article] [PubMed]
  • Lussier M, White AM, Sheraton J, di Paolo T, Treadwell J, Southard SB, Horenstein CI, Chen-Weiner J, Ram AF, Kapteyn JC, et al. Large scale identification of genes involved in cell surface biosynthesis and architecture in Saccharomyces cerevisiae. Genetics. 1997 Oct;147(2):435–450. [PMC free article] [PubMed]
  • Choi JY, Stukey J, Hwang SY, Martin CE. Regulatory elements that control transcription activation and unsaturated fatty acid-mediated repression of the Saccharomyces cerevisiae OLE1 gene. J Biol Chem. 1996 Feb 16;271(7):3581–3589. [PubMed]
  • Madison JM, Dudley AM, Winston F. Identification and analysis of Mot3, a zinc finger protein that binds to the retrotransposon Ty long terminal repeat (delta) in Saccharomyces cerevisiae. Mol Cell Biol. 1998 Apr;18(4):1879–1890. [PMC free article] [PubMed]
  • Crowley JH, Leak FW, Jr, Shianna KV, Tove S, Parks LW. A mutation in a purported regulatory gene affects control of sterol uptake in Saccharomyces cerevisiae. J Bacteriol. 1998 Aug;180(16):4177–4183. [PMC free article] [PubMed]
  • Sertil O, Cohen BD, Davies KJ, Lowry CV. The DAN1 gene of S. cerevisiae is regulated in parallel with the hypoxic genes, but by a different mechanism. Gene. 1997 Jun 19;192(2):199–205. [PubMed]
  • Deckert J, Perini R, Balasubramanian B, Zitomer RS. Multiple elements and auto-repression regulate Rox1, a repressor of hypoxic genes in Saccharomyces cerevisiae. Genetics. 1995 Mar;139(3):1149–1158. [PMC free article] [PubMed]
  • ter Linde JJ, Liang H, Davis RW, Steensma HY, van Dijken JP, Pronk JT. Genome-wide transcriptional analysis of aerobic and anaerobic chemostat cultures of Saccharomyces cerevisiae. J Bacteriol. 1999 Dec;181(24):7409–7413. [PMC free article] [PubMed]
  • Donzeau M, Bourdineaud JP, Lauquin GJ. Regulation by low temperatures and anaerobiosis of a yeast gene specifying a putative GPI-anchored plasma membrane protein [corrected]. Mol Microbiol. 1996 Apr;20(2):449–459. [PubMed]
  • Thorsness M, Schafer W, D'Ari L, Rine J. Positive and negative transcriptional control by heme of genes encoding 3-hydroxy-3-methylglutaryl coenzyme A reductase in Saccharomyces cerevisiae. Mol Cell Biol. 1989 Dec;9(12):5702–5712. [PMC free article] [PubMed]
  • Drgon T, Sabová L, Nelson N, Kolarov J. ADP/ATP translocator is essential only for anaerobic growth of yeast Saccharomyces cerevisiae. FEBS Lett. 1991 Sep 9;289(2):159–162. [PubMed]
  • Turi TG, Loper JC. Multiple regulatory elements control expression of the gene encoding the Saccharomyces cerevisiae cytochrome P450, lanosterol 14 alpha-demethylase (ERG11). J Biol Chem. 1992 Jan 25;267(3):2046–2056. [PubMed]
  • Evangelista CC, Jr, Rodriguez Torres AM, Limbach MP, Zitomer RS. Rox3 and Rts1 function in the global stress response pathway in baker's yeast. Genetics. 1996 Apr;142(4):1083–1093. [PMC free article] [PubMed]
  • Winston F, Dollard C, Ricupero-Hovasse SL. Construction of a set of convenient Saccharomyces cerevisiae strains that are isogenic to S288C. Yeast. 1995 Jan;11(1):53–55. [PubMed]
  • Grishin AV, Rothenberg M, Downs MA, Blumer KJ. Mot3, a Zn finger transcription factor that modulates gene expression and attenuates mating pheromone signaling in Saccharomyces cerevisiae. Genetics. 1998 Jun;149(2):879–892. [PMC free article] [PubMed]
  • Zitomer RS, Lowry CV. Regulation of gene expression by oxygen in Saccharomyces cerevisiae. Microbiol Rev. 1992 Mar;56(1):1–11. [PMC free article] [PubMed]
  • Hodge MR, Kim G, Singh K, Cumsky MG. Inverse regulation of the yeast COX5 genes by oxygen and heme. Mol Cell Biol. 1989 May;9(5):1958–1964. [PMC free article] [PubMed]
  • Zitomer RS, Sellers JW, McCarter DW, Hastings GA, Wick P, Lowry CV. Elements involved in oxygen regulation of the Saccharomyces cerevisiae CYC7 gene. Mol Cell Biol. 1987 Jun;7(6):2212–2220. [PMC free article] [PubMed]
  • Keleher CA, Redd MJ, Schultz J, Carlson M, Johnson AD. Ssn6-Tup1 is a general repressor of transcription in yeast. Cell. 1992 Feb 21;68(4):709–719. [PubMed]

Articles from Genetics are provided here courtesy of Genetics Society of America

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

  • Compound
    Compound
    PubChem Compound links
  • Gene
    Gene
    Gene links
  • GEO Profiles
    GEO Profiles
    Related GEO records
  • HomoloGene
    HomoloGene
    HomoloGene links
  • MedGen
    MedGen
    Related information in MedGen
  • Pathways + GO
    Pathways + GO
    Pathways, annotations and biological systems (BioSystems) that cite the current article.
  • Protein
    Protein
    Published protein sequences
  • PubMed
    PubMed
    PubMed citations for these articles
  • Substance
    Substance
    PubChem Substance links
  • Taxonomy
    Taxonomy
    Related taxonomy entry
  • Taxonomy Tree
    Taxonomy Tree

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...