• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of geneticsGeneticsCurrent IssueInformation for AuthorsEditorial BoardSubscribeSubmit a Manuscript
Genetics. Sep 2000; 156(1): 327–339.
PMCID: PMC1461246

Production and characterization of maize chromosome 9 radiation hybrids derived from an oat-maize addition line.

Abstract

In maize (Zea mays L., 2n = 2x = 20), map-based cloning and genome organization studies are often complicated because of the complexity of the genome. Maize chromosome addition lines of hexaploid cultivated oat (Avena sativa L., 2n = 6x = 42), where maize chromosomes can be individually manipulated, represent unique materials for maize genome analysis. Maize chromosome addition lines are particularly suitable for the dissection of a single maize chromosome using radiation because cultivated oat is an allohexaploid in which multiple copies of the oat basic genome provide buffering to chromosomal aberrations and other mutations. Irradiation (gamma rays at 30, 40, and 50 krad) of a monosomic maize chromosome 9 addition line produced maize chromosome 9 radiation hybrids (M9RHs)-oat lines possessing different fragments of maize chromosome 9 including intergenomic translocations and modified maize addition chromosomes with internal and terminal deletions. M9RHs with 1 to 10 radiation-induced breaks per chromosome were identified. We estimated that a panel of 100 informative M9RHs (with an average of 3 breaks per chromosome) would allow mapping at the 0. 5- to 1.0-Mb level of resolution. Because mapping with maize chromosome addition lines and radiation hybrid derivatives involves assays for the presence or absence of a given marker, monomorphic markers can be quickly and efficiently mapped to a chromosome region. Radiation hybrid derivatives also represent sources of region-specific DNA for cloning of genes or DNA markers.

Full Text

The Full Text of this article is available as a PDF (999K).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Ananiev EV, Riera-Lizarazu O, Rines HW, Phillips RL. Oat-maize chromosome addition lines: a new system for mapping the maize genome. Proc Natl Acad Sci U S A. 1997 Apr 15;94(8):3524–3529. [PMC free article] [PubMed]
  • Ananiev EV, Phillips RL, Rines HW. Complex structure of knob DNA on maize chromosome 9. Retrotransposon invasion into heterochromatin. Genetics. 1998 Aug;149(4):2025–2037. [PMC free article] [PubMed]
  • Ananiev EV, Phillips RL, Rines HW. Chromosome-specific molecular organization of maize (Zea mays L.) centromeric regions. Proc Natl Acad Sci U S A. 1998 Oct 27;95(22):13073–13078. [PMC free article] [PubMed]
  • Ananiev EV, Phillips RL, Rines HW. A knob-associated tandem repeat in maize capable of forming fold-back DNA segments: are chromosome knobs megatransposons? Proc Natl Acad Sci U S A. 1998 Sep 1;95(18):10785–10790. [PMC free article] [PubMed]
  • Bass HW, Riera-Lizarazu O, Ananiev EV, Bordoli SJ, Rines HW, Phillips RL, Sedat JW, Agard DA, Cande WZ. Evidence for the coincident initiation of homolog pairing and synapsis during the telomere-clustering (bouquet) stage of meiotic prophase. J Cell Sci. 2000 Mar;113(Pt 6):1033–1042. [PubMed]
  • Bennett MD, Smith JB. Nuclear dna amounts in angiosperms. Philos Trans R Soc Lond B Biol Sci. 1976 May 27;274(933):227–274. [PubMed]
  • Bennetzen JL, Freeling M. Grasses as a single genetic system: genome composition, collinearity and compatibility. Trends Genet. 1993 Aug;9(8):259–261. [PubMed]
  • Bennetzen JL, Schrick K, Springer PS, Brown WE, SanMiguel P. Active maize genes are unmodified and flanked by diverse classes of modified, highly repetitive DNA. Genome. 1994 Aug;37(4):565–576. [PubMed]
  • Chumakov IM, Rigault P, Le Gall I, Bellanné-Chantelot C, Billault A, Guillou S, Soularue P, Guasconi G, Poullier E, Gros I, et al. A YAC contig map of the human genome. Nature. 1995 Sep 28;377(6547 Suppl):175–297. [PubMed]
  • Cox DR, Burmeister M, Price ER, Kim S, Myers RM. Radiation hybrid mapping: a somatic cell genetic method for constructing high-resolution maps of mammalian chromosomes. Science. 1990 Oct 12;250(4978):245–250. [PubMed]
  • Deloukas P, Schuler GD, Gyapay G, Beasley EM, Soderlund C, Rodriguez-Tomé P, Hui L, Matise TC, McKusick KB, Beckmann JS, et al. A physical map of 30,000 human genes. Science. 1998 Oct 23;282(5389):744–746. [PubMed]
  • Dib C, Fauré S, Fizames C, Samson D, Drouot N, Vignal A, Millasseau P, Marc S, Hazan J, Seboun E, et al. A comprehensive genetic map of the human genome based on 5,264 microsatellites. Nature. 1996 Mar 14;380(6570):152–154. [PubMed]
  • Driscoll CJ, Jensen NF. A Genetic Method for Detecting Induced Intergeneric Translocations. Genetics. 1963 Apr;48(4):459–468. [PMC free article] [PubMed]
  • Edwards KJ, Thompson H, Edwards D, de Saizieu A, Sparks C, Thompson JA, Greenland AJ, Eyers M, Schuch W. Construction and characterisation of a yeast artificial chromosome library containing three haploid maize genome equivalents. Plant Mol Biol. 1992 May;19(2):299–308. [PubMed]
  • Flavell RB. Repetitive DNA and chromosome evolution in plants. Philos Trans R Soc Lond B Biol Sci. 1986 Jan 29;312(1154):227–242. [PubMed]
  • Foote T, Roberts M, Kurata N, Sasaki T, Moore G. Detailed comparative mapping of cereal chromosome regions corresponding to the Ph1 locus in wheat. Genetics. 1997 Oct;147(2):801–807. [PMC free article] [PubMed]
  • Goss SJ, Harris H. New method for mapping genes in human chromosomes. Nature. 1975 Jun 26;255(5511):680–684. [PubMed]
  • Gyapay G, Schmitt K, Fizames C, Jones H, Vega-Czarny N, Spillett D, Muselet D, Prud'homme JF, Dib C, Auffray C, et al. A radiation hybrid map of the human genome. Hum Mol Genet. 1996 Mar;5(3):339–346. [PubMed]
  • Priat C, Hitte C, Vignaux F, Renier C, Jiang Z, Jouquand S, Chéron A, André C, Galibert F. A whole-genome radiation hybrid map of the dog genome. Genomics. 1998 Dec 15;54(3):361–378. [PubMed]
  • Pritchard CA, Casher D, Uglum E, Cox DR, Myers RM. Isolation and field-inversion gel electrophoresis analysis of DNA markers located close to the Huntington disease gene. Genomics. 1989 Apr;4(3):408–418. [PubMed]
  • Hawken RJ, Murtaugh J, Flickinger GH, Yerle M, Robic A, Milan D, Gellin J, Beattie CW, Schook LB, Alexander LJ. A first-generation porcine whole-genome radiation hybrid map. Mamm Genome. 1999 Aug;10(8):824–830. [PubMed]
  • Hong G, Qian Y, Yu S, Hu X, Zhu J, Tao W, Li W, Su C, Zhao H, Qiu L, et al. A 120 kilobase resolution contig map of the rice genome. DNA Seq. 1997;7(6):319–335. [PubMed]
  • Hudson TJ, Stein LD, Gerety SS, Ma J, Castle AB, Silva J, Slonim DK, Baptista R, Kruglyak L, Xu SH, et al. An STS-based map of the human genome. Science. 1995 Dec 22;270(5244):1945–1954. [PubMed]
  • Sachs RK, Chen AM, Brenner DJ. Review: proximity effects in the production of chromosome aberrations by ionizing radiation. Int J Radiat Biol. 1997 Jan;71(1):1–19. [PubMed]
  • SanMiguel P, Tikhonov A, Jin YK, Motchoulskaia N, Zakharov D, Melake-Berhan A, Springer PS, Edwards KJ, Lee M, Avramova Z, et al. Nested retrotransposons in the intergenic regions of the maize genome. Science. 1996 Nov 1;274(5288):765–768. [PubMed]
  • Kodama Y, Nakano M, Ohtaki K, Delongchamp R, Awa AA, Nakamura N. Estimation of minimal size of translocated chromosome segments detectable by fluorescence in situ hybridization. Int J Radiat Biol. 1997 Jan;71(1):35–39. [PubMed]
  • Schmidt R, West J, Love K, Lenehan Z, Lister C, Thompson H, Bouchez D, Dean C. Physical map and organization of Arabidopsis thaliana chromosome 4. Science. 1995 Oct 20;270(5235):480–483. [PubMed]
  • Kurata N, Umehara Y, Tanoue H, Sasaki T. Physical mapping of the rice genome with YAC clones. Plant Mol Biol. 1997 Sep;35(1-2):101–113. [PubMed]
  • Schuler GD, Boguski MS, Stewart EA, Stein LD, Gyapay G, Rice K, White RE, Rodriguez-Tomé P, Aggarwal A, Bajorek E, et al. A gene map of the human genome. Science. 1996 Oct 25;274(5287):540–546. [PubMed]
  • Ledbetter SA, Nelson DL, Warren ST, Ledbetter DH. Rapid isolation of DNA probes within specific chromosome regions by interspersed repetitive sequence polymerase chain reaction. Genomics. 1990 Mar;6(3):475–481. [PubMed]
  • Simpson PJ, Savage JR. Dose-response curves for simple and complex chromosome aberrations induced by X-rays and detected using fluorescence in situ hybridization. Int J Radiat Biol. 1996 Apr;69(4):429–436. [PubMed]
  • Stewart EA, McKusick KB, Aggarwal A, Bajorek E, Brady S, Chu A, Fang N, Hadley D, Harris M, Hussain S, et al. An STS-based radiation hybrid map of the human genome. Genome Res. 1997 May;7(5):422–433. [PubMed]
  • Vignaux F, Hitte C, Priat C, Chuat JC, Andre C, Galibert F. Construction and optimization of a dog whole-genome radiation hybrid panel. Mamm Genome. 1999 Sep;10(9):888–894. [PubMed]
  • McCarthy LC, Terrett J, Davis ME, Knights CJ, Smith AL, Critcher R, Schmitt K, Hudson J, Spurr NK, Goodfellow PN. A first-generation whole genome-radiation hybrid map spanning the mouse genome. Genome Res. 1997 Dec;7(12):1153–1161. [PMC free article] [PubMed]
  • Watanabe TK, Bihoreau MT, McCarthy LC, Kiguwa SL, Hishigaki H, Tsuji A, Browne J, Yamasaki Y, Mizoguchi-Miyakita A, Oga K, et al. A radiation hybrid map of the rat genome containing 5,255 markers. Nat Genet. 1999 May;22(1):27–36. [PubMed]
  • Yerle M, Pinton P, Robic A, Alfonso A, Palvadeau Y, Delcros C, Hawken R, Alexander L, Beattie C, Schook L, et al. Construction of a whole-genome radiation hybrid panel for high-resolution gene mapping in pigs. Cytogenet Cell Genet. 1998;82(3-4):182–188. [PubMed]
  • Yokota H, van den Engh G, Hearst JE, Sachs RK, Trask BJ. Evidence for the organization of chromatin in megabase pair-sized loops arranged along a random walk path in the human G0/G1 interphase nucleus. J Cell Biol. 1995 Sep;130(6):1239–1249. [PMC free article] [PubMed]
  • Zachgo EA, Wang ML, Dewdney J, Bouchez D, Camilleri C, Belmonte S, Huang L, Dolan M, Goodman HM. A physical map of chromosome 2 of Arabidopsis thaliana. Genome Res. 1996 Jan;6(1):19–25. [PubMed]
  • Paterson AH, Lin YR, Li Z, Schertz KF, Doebley JF, Pinson SR, Liu SC, Stansel JW, Irvine JE. Convergent domestication of cereal crops by independent mutations at corresponding genetic Loci. Science. 1995 Sep 22;269(5231):1714–1718. [PubMed]

Articles from Genetics are provided here courtesy of Genetics Society of America

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...