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Genetics. Aug 2000; 155(4): 1773–1788.
PMCID: PMC1461186

Quantitative trait loci for life span in Drosophila melanogaster: interactions with genetic background and larval density.

Abstract

The genetic architecture of variation in adult life span was examined for a population of recombinant inbred lines, each of which had been crossed to both inbred parental strains from which the lines were derived, after emergence from both high and low larval density. QTL affecting life span were mapped within each sex and larval density treatment by linkage to highly polymorphic roo-transposable element markers, using a composite interval mapping method. We detected a total of six QTL affecting life span; the additive effects and degrees of dominance for all were highly sex- and larval environment-specific. There were significant epistatic interactions between five of the life span QTL, the effects of which also differed according to genetic background, sex, and larval density. Five additional QTL were identified that contributed to differences among lines in their sensitivity to variation in larval density. Further fine-scale mapping is necessary to determine whether candidate genes within the regions to which the QTL map are actually responsible for the observed variation in life span.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • David JR, Bocquet C, Arens MF, Fouillet P. Biological role of alcohol dehydrogenase in the tolerance of Drosophila melanogaster to aliphatic alochols: utilization of an ADH-null mutant. Biochem Genet. 1976 Dec;14(11-12):989–997. [PubMed]
  • Doerge RW, Churchill GA. Permutation tests for multiple loci affecting a quantitative character. Genetics. 1996 Jan;142(1):285–294. [PMC free article] [PubMed]
  • Duckworth WC, Bennett RG, Hamel FG. A direct inhibitory effect of insulin on a cytosolic proteolytic complex containing insulin-degrading enzyme and multicatalytic proteinase. J Biol Chem. 1994 Oct 7;269(40):24575–24580. [PubMed]
  • Dudas SP, Arking R. A coordinate upregulation of antioxidant gene activities is associated with the delayed onset of senescence in a long-lived strain of Drosophila. J Gerontol A Biol Sci Med Sci. 1995 May;50(3):B117–B127. [PubMed]
  • Friedman DB, Johnson TE. A mutation in the age-1 gene in Caenorhabditis elegans lengthens life and reduces hermaphrodite fertility. Genetics. 1988 Jan;118(1):75–86. [PMC free article] [PubMed]
  • Fry JD, Nuzhdin SV, Pasyukova EG, Mackay TF. QTL mapping of genotype-environment interaction for fitness in Drosophila melanogaster. Genet Res. 1998 Apr;71(2):133–141. [PubMed]
  • Gagny B, Rossignol M, Silar P. Cloning, sequencing, and transgenic expression of Podospora curvicolla and Sordaria macrospora eEF1A genes: relationship between cytosolic translation and longevity in filamentous fungi. Fungal Genet Biol. 1997 Dec;22(3):191–198. [PubMed]
  • Austad SN. The uses of intraspecific variation in aging research. Exp Gerontol. 1996 Jul-Aug;31(4):453–463. [PubMed]
  • Garcia JV, Fenton BW, Rosner MR. Isolation and characterization of an insulin-degrading enzyme from Drosophila melanogaster. Biochemistry. 1988 Jun 14;27(12):4237–4244. [PubMed]
  • Austad SN. Comparative aging and life histories in mammals. Exp Gerontol. 1997 Jan-Apr;32(1-2):23–38. [PubMed]
  • Gems D, Sutton AJ, Sundermeyer ML, Albert PS, King KV, Edgley ML, Larsen PL, Riddle DL. Two pleiotropic classes of daf-2 mutation affect larval arrest, adult behavior, reproduction and longevity in Caenorhabditis elegans. Genetics. 1998 Sep;150(1):129–155. [PMC free article] [PubMed]
  • Bennett RG, Hamel FG, Duckworth WC. Characterization of the insulin inhibition of the peptidolytic activities of the insulin-degrading enzyme-proteasome complex. Diabetes. 1997 Feb;46(2):197–203. [PubMed]
  • Graf JD, Ayala FJ. Genetic variation for superoxide dismutase level in Drosophila melanogaster. Biochem Genet. 1986 Apr;24(3-4):153–168. [PubMed]
  • Graves JL, Jr, Mueller LD. Population density effects on longevity. Genetica. 1993;91(1-3):99–109. [PubMed]
  • Bourguet D, Prout M, Raymond M. Dominance of insecticide resistance presents a plastic response. Genetics. 1996 May;143(1):407–416. [PMC free article] [PubMed]
  • Halushka MK, Fan JB, Bentley K, Hsie L, Shen N, Weder A, Cooper R, Lipshutz R, Chakravarti A. Patterns of single-nucleotide polymorphisms in candidate genes for blood-pressure homeostasis. Nat Genet. 1999 Jul;22(3):239–247. [PubMed]
  • Buck S, Nicholson M, Dudas S, Wells R, Force A, Baker GT, 3rd, Arking R. Larval regulation of adult longevity in a genetically-selected long-lived strain of Drosophila. Heredity (Edinb) 1993 Jul;71(Pt 1):23–32. [PubMed]
  • Hamilton WD. The moulding of senescence by natural selection. J Theor Biol. 1966 Sep;12(1):12–45. [PubMed]
  • Hari R, Burde V, Arking R. Immunological confirmation of elevated levels of CuZn superoxide dismutase protein in an artificially selected long-lived strain of Drosophila melanogaster. Exp Gerontol. 1998 May;33(3):227–237. [PubMed]
  • Cargill M, Altshuler D, Ireland J, Sklar P, Ardlie K, Patil N, Shaw N, Lane CR, Lim EP, Kalyanaraman N, et al. Characterization of single-nucleotide polymorphisms in coding regions of human genes. Nat Genet. 1999 Jul;22(3):231–238. [PubMed]
  • Heinstra PW, Geer BW. Metabolic control analysis and enzyme variation: nutritional manipulation of the flux from ethanol to lipids in Drosophila. Mol Biol Evol. 1991 Sep;8(5):703–708. [PubMed]
  • Hekimi S, Lakowski B, Barnes TM, Ewbank JJ. Molecular genetics of life span in C. elegans: how much does it teach us? Trends Genet. 1998 Jan;14(1):14–20. [PubMed]
  • Houle D. Comparing evolvability and variability of quantitative traits. Genetics. 1992 Jan;130(1):195–204. [PMC free article] [PubMed]
  • Hsin H, Kenyon C. Signals from the reproductive system regulate the lifespan of C. elegans. Nature. 1999 May 27;399(6734):362–366. [PubMed]
  • Jazwinski SM. Longevity, genes, and aging. Science. 1996 Jul 5;273(5271):54–59. [PubMed]
  • Kao CH, Zeng ZB, Teasdale RD. Multiple interval mapping for quantitative trait loci. Genetics. 1999 Jul;152(3):1203–1216. [PMC free article] [PubMed]
  • Kenyon C, Chang J, Gensch E, Rudner A, Tabtiang R. A C. elegans mutant that lives twice as long as wild type. Nature. 1993 Dec 2;366(6454):461–464. [PubMed]
  • Risch N, Merikangas K. The future of genetic studies of complex human diseases. Science. 1996 Sep 13;273(5281):1516–1517. [PubMed]
  • Kirchman PA, Kim S, Lai CY, Jazwinski SM. Interorganelle signaling is a determinant of longevity in Saccharomyces cerevisiae. Genetics. 1999 May;152(1):179–190. [PMC free article] [PubMed]
  • Lai C, Lyman RF, Long AD, Langley CH, Mackay TF. Naturally occurring variation in bristle number and DNA polymorphisms at the scabrous locus of Drosophila melanogaster. Science. 1994 Dec 9;266(5191):1697–1702. [PubMed]
  • Larsen PL, Albert PS, Riddle DL. Genes that regulate both development and longevity in Caenorhabditis elegans. Genetics. 1995 Apr;139(4):1567–1583. [PMC free article] [PubMed]
  • Shama S, Lai CY, Antoniazzi JM, Jiang JC, Jazwinski SM. Heat stress-induced life span extension in yeast. Exp Cell Res. 1998 Dec 15;245(2):379–388. [PubMed]
  • Sinclair D, Mills K, Guarente L. Aging in Saccharomyces cerevisiae. Annu Rev Microbiol. 1998;52:533–560. [PubMed]
  • Long AD, Mullaney SL, Mackay TF, Langley CH. Genetic interactions between naturally occurring alleles at quantitative trait loci and mutant alleles at candidate loci affecting bristle number in Drosophila melanogaster. Genetics. 1996 Dec;144(4):1497–1510. [PMC free article] [PubMed]
  • Long AD, Lyman RF, Langley CH, Mackay TF. Two sites in the Delta gene region contribute to naturally occurring variation in bristle number in Drosophila melanogaster. Genetics. 1998 Jun;149(2):999–1017. [PMC free article] [PubMed]
  • Taub J, Lau JF, Ma C, Hahn JH, Hoque R, Rothblatt J, Chalfie M. A cytosolic catalase is needed to extend adult lifespan in C. elegans daf-C and clk-1 mutants. Nature. 1999 May 13;399(6732):162–166. [PubMed]
  • Luckinbill LS, Riha V, Rhine S, Grudzien TA. The role of glucose-6-phosphate dehydrogenase in the evolution of longevity in Drosophila melanogaster. Heredity (Edinb) 1990 Aug;65(Pt 1):29–38. [PubMed]
  • Vaupel JW, Carey JR, Christensen K, Johnson TE, Yashin AI, Holm NV, Iachine IA, Kannisto V, Khazaeli AA, Liedo P, et al. Biodemographic trajectories of longevity. Science. 1998 May 8;280(5365):855–860. [PubMed]
  • Mackay TF, Langley CH. Molecular and phenotypic variation in the achaete-scute region of Drosophila melanogaster. Nature. 1990 Nov 1;348(6296):64–66. [PubMed]
  • Mackay TF, Fry JD. Polygenic mutation in Drosophila melanogaster: genetic interactions between selection lines and candidate quantitative trait loci. Genetics. 1996 Oct;144(2):671–688. [PMC free article] [PubMed]
  • Vieira C, Pasyukova EG, Zeng ZB, Hackett JB, Lyman RF, Mackay TF. Genotype-environment interaction for quantitative trait loci affecting life span in Drosophila melanogaster. Genetics. 2000 Jan;154(1):213–227. [PMC free article] [PubMed]
  • Martínez DE. Mortality patterns suggest lack of senescence in hydra. Exp Gerontol. 1998 May;33(3):217–225. [PubMed]
  • Wallace DC, Melov S. Radicals r'aging. Nat Genet. 1998 Jun;19(2):105–106. [PubMed]
  • Morimoto RI, Kline MP, Bimston DN, Cotto JJ. The heat-shock response: regulation and function of heat-shock proteins and molecular chaperones. Essays Biochem. 1997;32:17–29. [PubMed]
  • Nuzhdin SV, Pasyukova EG, Dilda CL, Zeng ZB, Mackay TF. Sex-specific quantitative trait loci affecting longevity in Drosophila melanogaster. Proc Natl Acad Sci U S A. 1997 Sep 2;94(18):9734–9739. [PMC free article] [PubMed]
  • Orr WC, Sohal RS. Extension of life-span by overexpression of superoxide dismutase and catalase in Drosophila melanogaster. Science. 1994 Feb 25;263(5150):1128–1130. [PubMed]
  • Zeng ZB. Precision mapping of quantitative trait loci. Genetics. 1994 Apr;136(4):1457–1468. [PMC free article] [PubMed]
  • Parkes TL, Elia AJ, Dickinson D, Hilliker AJ, Phillips JP, Boulianne GL. Extension of Drosophila lifespan by overexpression of human SOD1 in motorneurons. Nat Genet. 1998 Jun;19(2):171–174. [PubMed]
  • Zwaan BJ, Bijlsma R, Hoekstra RF. On the developmental theory of ageing. I. starvation resistance and longevity in Drosophila melanogaster in relation to pre-adult breeding conditions. Heredity (Edinb) 1991 Feb;66(Pt 1):29–39. [PubMed]
  • Pasyukova EG, Nuzhdin SV. Doc and copia instability in an isogenic Drosophila melanogaster stock. Mol Gen Genet. 1993 Aug;240(2):302–306. [PubMed]

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