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Genetics. 2000 Mar; 154(3): 1213–1229.
PMCID: PMC1460985

A genetic screen for zygotic embryonic lethal mutations affecting cuticular morphology in the wasp Nasonia vitripennis.


We have screened for zygotic embryonic lethal mutations affecting cuticular morphology in Nasonia vitripennis (Hymenoptera; Chalcidoidea). Our broad goal was to investigate the use of Nasonia for genetically surveying conservation and change in regulatory gene systems, as a means to understand the diversity of developmental strategies that have arisen during the course of evolution. Specifically, we aim to compare anteroposterior patterning gene functions in two long germ band insects, Nasonia and Drosophila. In Nasonia, unfertilized eggs develop as haploid males while fertilized eggs develop as diploid females, so the entire genome can be screened for recessive zygotic mutations by examining the progeny of F1 females. We describe 74 of >100 lines with embryonic cuticular mutant phenotypes, including representatives of coordinate, gap, pair-rule, segment polarity, homeotic, and Polycomb group functions, as well as mutants with novel phenotypes not directly comparable to those of known Drosophila genes. We conclude that Nasonia is a tractable experimental organism for comparative developmental genetic study. The mutants isolated here have begun to outline the extent of conservation and change in the genetic programs controlling embryonic patterning in Nasonia and Drosophila.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Kelsh R, Weinzierl RO, White RA, Akam M. Homeotic gene expression in the locust Schistocerca: an antibody that detects conserved epitopes in Ultrabithorax and abdominal-A proteins. Dev Genet. 1994;15(1):19–31. [PubMed]
  • Kennerdell JR, Carthew RW. Use of dsRNA-mediated genetic interference to demonstrate that frizzled and frizzled 2 act in the wingless pathway. Cell. 1998 Dec 23;95(7):1017–1026. [PubMed]
  • Lehmann R, Nüsslein-Volhard C. hunchback, a gene required for segmentation of an anterior and posterior region of the Drosophila embryo. Dev Biol. 1987 Feb;119(2):402–417. [PubMed]
  • Macdonald PM, Struhl G. A molecular gradient in early Drosophila embryos and its role in specifying the body pattern. Nature. 1986 Dec 11;324(6097):537–545. [PubMed]
  • Berghammer A, Bucher G, Maderspacher F, Klingler M. A system to efficiently maintain embryonic lethal mutations in the flour beetle Tribolium castaneum. Dev Genes Evol. 1999 Jun;209(6):382–389. [PubMed]
  • Macdonald PM, Ingham P, Struhl G. Isolation, structure, and expression of even-skipped: a second pair-rule gene of Drosophila containing a homeo box. Cell. 1986 Dec 5;47(5):721–734. [PubMed]
  • Beukeboom LW. Sex determination in Hymenoptera: a need for genetic and molecular studies. Bioessays. 1995 Sep;17(9):813–817. [PubMed]
  • Maderspacher F, Bucher G, Klingler M. Pair-rule and gap gene mutants in the flour beetle Tribolium castaneum. Dev Genes Evol. 1998 Dec;208(10):558–568. [PubMed]
  • Brown SJ, Hilgenfeld RB, Denell RE. The beetle Tribolium castaneum has a fushi tarazu homolog expressed in stripes during segmentation. Proc Natl Acad Sci U S A. 1994 Dec 20;91(26):12922–12926. [PMC free article] [PubMed]
  • Mazur P, Cole KW, Hall JW, Schreuders PD, Mahowald AP. Cryobiological preservation of Drosophila embryos. Science. 1992 Dec 18;258(5090):1932–1935. [PubMed]
  • Brown SJ, Mahaffey JP, Lorenzen MD, Denell RE, Mahaffey JW. Using RNAi to investigate orthologous homeotic gene function during development of distantly related insects. Evol Dev. 1999 Jul-Aug;1(1):11–15. [PubMed]
  • Murtha MT, Leckman JF, Ruddle FH. Detection of homeobox genes in development and evolution. Proc Natl Acad Sci U S A. 1991 Dec 1;88(23):10711–10715. [PMC free article] [PubMed]
  • Nüsslein-Volhard C, Wieschaus E. Mutations affecting segment number and polarity in Drosophila. Nature. 1980 Oct 30;287(5785):795–801. [PubMed]
  • Denell RE, Frederick RD. Homoeosis in Drosophila: a description of the Polycomb lethal syndrome. Dev Biol. 1983 May;97(1):34–47. [PubMed]
  • Patel NH, Martin-Blanco E, Coleman KG, Poole SJ, Ellis MC, Kornberg TB, Goodman CS. Expression of engrailed proteins in arthropods, annelids, and chordates. Cell. 1989 Sep 8;58(5):955–968. [PubMed]
  • Perrimon N, Engstrom L, Mahowald AP. Developmental genetics of the 2E-F region of the Drosophila X chromosome: a region rich in "developmentally important" genes. Genetics. 1984 Nov;108(3):559–572. [PMC free article] [PubMed]
  • DiNardo S, Kuner JM, Theis J, O'Farrell PH. Development of embryonic pattern in D. melanogaster as revealed by accumulation of the nuclear engrailed protein. Cell. 1985 Nov;43(1):59–69. [PMC free article] [PubMed]
  • Petschek JP, Perrimon N, Mahowald AP. Region-specific defects in l(1)giant embryos of Drosophila melanogaster. Dev Biol. 1987 Jan;119(1):175–189. [PubMed]
  • Dobson SL, Tanouye MA. Evidence for a genomic imprinting sex determination mechanism in Nasonia vitripennis (Hymenoptera; Chalcidoidea). Genetics. 1998 May;149(1):233–242. [PMC free article] [PubMed]
  • Pultz MA, Pitt JN, Alto NM. Extensive zygotic control of the anteroposterior axis in the wasp Nasonia vitripennis. Development. 1999 Feb;126(4):701–710. [PubMed]
  • Eizinger A, Jungblut B, Sommer RJ. Evolutionary change in the functional specificity of genes. Trends Genet. 1999 May;15(5):197–202. [PubMed]
  • Rogers BT, Kaufman TC. Structure of the insect head as revealed by the EN protein pattern in developing embryos. Development. 1996 Nov;122(11):3419–3432. [PubMed]
  • Gadau J, Page RE, Jr, Werren JH. Mapping of hybrid incompatibility loci in Nasonia. Genetics. 1999 Dec;153(4):1731–1741. [PMC free article] [PubMed]
  • Grbić M, Strand MR. Shifts in the life history of parasitic wasps correlate with pronounced alterations in early development. Proc Natl Acad Sci U S A. 1998 Feb 3;95(3):1097–1101. [PMC free article] [PubMed]
  • Grbic M, Nagy LM, Carroll SB, Strand M. Polyembryonic development: insect pattern formation in a cellularized environment. Development. 1996 Mar;122(3):795–804. [PubMed]
  • Grbić M, Nagy LM, Strand MR. Development of polyembryonic insects: a major departure from typical insect embryogenesis. Dev Genes Evol. 1998 Apr;208(2):69–81. [PubMed]
  • Schüpbach T, Wieschaus E. Female sterile mutations on the second chromosome of Drosophila melanogaster. I. Maternal effect mutations. Genetics. 1989 Jan;121(1):101–117. [PMC free article] [PubMed]
  • Hunter CP. Genetics: a touch of elegance with RNAi. Curr Biol. 1999 Jun 17;9(12):R440–R442. [PubMed]
  • Ingham PW, Ish-Horowicz D, Howard KR. Correlative changes in homoeotic and segmentation gene expression in Krüppel mutant embryos of Drosophila. EMBO J. 1986 Jul;5(7):1659–1665. [PMC free article] [PubMed]
  • Shippy TD, Brown SJ, Denell RE. Molecular characterization of the Tribolium abdominal-A ortholog and implications for the products of the Drosophila gene. Dev Genes Evol. 1998 Jan;207(7):446–452. [PubMed]
  • Simpson-Brose M, Treisman J, Desplan C. Synergy between the hunchback and bicoid morphogens is required for anterior patterning in Drosophila. Cell. 1994 Sep 9;78(5):855–865. [PubMed]
  • Wedeen C, Harding K, Levine M. Spatial regulation of Antennapedia and bithorax gene expression by the Polycomb locus in Drosophila. Cell. 1986 Mar 14;44(5):739–748. [PubMed]
  • Sommer RJ, Retzlaff M, Goerlich K, Sander K, Tautz D. Evolutionary conservation pattern of zinc-finger domains of Drosophila segmentation genes. Proc Natl Acad Sci U S A. 1992 Nov 15;89(22):10782–10786. [PMC free article] [PubMed]
  • Stauber M, Jäckle H, Schmidt-Ott U. The anterior determinant bicoid of Drosophila is a derived Hox class 3 gene. Proc Natl Acad Sci U S A. 1999 Mar 30;96(7):3786–3789. [PMC free article] [PubMed]
  • Strand MR, Grbic M. The development and evolution of polyembryonic insects. Curr Top Dev Biol. 1997;35:121–159. [PubMed]
  • Stuart JJ, Brown SJ, Beeman RW, Denell RE. A deficiency of the homeotic complex of the beetle Tribolium. Nature. 1991 Mar 7;350(6313):72–74. [PubMed]

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