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Genetics. Dec 1999; 153(4): 1909–1918.
PMCID: PMC1460846

Induction and characterization of Ph1 wheat mutants.


The cloning of genes for complex traits in polyploid plants that possess large genomes, such as hexaploid wheat, requires an efficient strategy. We present here one such strategy focusing on the homologous pairing suppressor (Ph1) locus of wheat. This locus has been shown to affect both premeiotic and meiotic processes, possibly suggesting a complex control. The strategy combined the identification of lines carrying specific deletions using multiplex PCR screening of fast-neutron irradiated wheat populations with the approach of physically mapping the region in the rice genome equivalent to the deletion to reveal its gene content. As a result, we have located the Ph1 factor controlling the euploid-like level of homologous chromosome pairing to the region between two loci (Xrgc846 and Xpsr150A). These loci are located within 400 kb of each other in the rice genome. By sequencing this region of the rice genome, it should now be possible to define the nature of this factor.

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Selected References

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  • Aragón-Alcaide L, Reader S, Beven A, Shaw P, Miller T, Moore G. Association of homologous chromosomes during floral development. Curr Biol. 1997 Nov 1;7(11):905–908. [PubMed]
  • Aragón-Alcaide L, Reader S, Miller T, Moore G. Centromeric behaviour in wheat with high and low homoeologous chromosomal pairing. Chromosoma. 1997 Oct;106(5):327–333. [PubMed]
  • Burke DT, Carle GF, Olson MV. Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vectors. Science. 1987 May 15;236(4803):806–812. [PubMed]
  • Ceoloni C, Donini P. Combining mutations for the two homoeologous pairing suppressor genes Ph1 and Ph2 in common wheat and in hybrids with alien Triticeae. Genome. 1993 Apr;36(2):377–386. [PubMed]
  • Dubcovsky J, Luo M, Dvorak J. Differentiation between homoeologous chromosomes 1A of wheat and 1Am of Triticum monococcum and its recognition by the wheat Ph1 locus. Proc Natl Acad Sci U S A. 1995 Jul 3;92(14):6645–6649. [PMC free article] [PubMed]
  • Dunford RP, Kurata N, Laurie DA, Money TA, Minobe Y, Moore G. Conservation of fine-scale DNA marker order in the genomes of rice and the Triticeae. Nucleic Acids Res. 1995 Jul 25;23(14):2724–2728. [PMC free article] [PubMed]
  • Foote T, Roberts M, Kurata N, Sasaki T, Moore G. Detailed comparative mapping of cereal chromosome regions corresponding to the Ph1 locus in wheat. Genetics. 1997 Oct;147(2):801–807. [PMC free article] [PubMed]
  • Gill KS, Gill BS, Endo TR, Mukai Y. Fine physical mapping of Ph1, a chromosome pairing regulator gene in polyploid wheat. Genetics. 1993 Aug;134(4):1231–1236. [PMC free article] [PubMed]
  • Gill KS, Gill BS, Endo TR, Boyko EV. Identification and high-density mapping of gene-rich regions in chromosome group 5 of wheat. Genetics. 1996 Jun;143(2):1001–1012. [PMC free article] [PubMed]
  • Ji LH, Langridge P. An early meiosis cDNA clone from wheat. Mol Gen Genet. 1994 Apr;243(1):17–23. [PubMed]
  • Kurata N, Nagamura Y, Yamamoto K, Harushima Y, Sue N, Wu J, Antonio BA, Shomura A, Shimizu T, Lin SY, et al. A 300 kilobase interval genetic map of rice including 883 expressed sequences. Nat Genet. 1994 Dec;8(4):365–372. [PubMed]
  • Silverman GA. Isolating vector-insert junctions from yeast artificial chromosomes. PCR Methods Appl. 1993 Dec;3(3):141–150. [PubMed]
  • Luo MC, Dubcovsky J, Dvorák J. Recognition of homeology by the wheat Ph1 locus. Genetics. 1996 Nov;144(3):1195–1203. [PMC free article] [PubMed]
  • Martínez-Pérez E, Shaw P, Reader S, Aragón-Alcaide L, Miller T, Moore G. Homologous chromosome pairing in wheat. J Cell Sci. 1999 Jun;112(Pt 11):1761–1769. [PubMed]
  • Vega JM, Feldman M. Effect of the pairing gene Ph1 and premeiotic colchicine treatment on intra- and interchromosome pairing of isochromosomes in common wheat. Genetics. 1998 Nov;150(3):1199–1208. [PMC free article] [PubMed]
  • Vos P, Hogers R, Bleeker M, Reijans M, van de Lee T, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M, et al. AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res. 1995 Nov 11;23(21):4407–4414. [PMC free article] [PubMed]

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