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Genetics. Feb 1999; 151(2): 803–820.
PMCID: PMC1460484

A second-generation genetic linkage map of the domestic dog, Canis familiaris.

Abstract

Purebred strains, pronounced phenotypic variation, and a high incidence of heritable disease make the domestic dog uniquely suited to complement genetic analyses in humans and mice. A comprehensive genetic linkage map would afford many opportunities in dogs, ranging from the positional cloning of disease genes to the dissection of quantitative differences in size, shape, and behavior. Here we report a canine linkage map with the number of mapped loci expanded to 276 and 10-cM coverage extended to 75-90% of the genome. Most of the 38 canine autosomes are likely represented in the collection of 39 autosomal linkage groups. Eight markers were sufficiently informative to detect linkage at distances of 10-13 cM, yet remained unlinked to any other marker. Taken together, the results suggested a genome size of about 27 M. As in other species, the genetic length varied between sexes, with the female autosomal distance being approximately 1.4-fold greater than that of male meioses. Fifteen markers anchored well-described genes on the map, thereby serving as landmarks for comparative mapping in dogs. We discuss the utility of the current map and outline steps necessary for future map improvement.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Acland GM, Ray K, Mellersh CS, Gu W, Langston AA, Rine J, Ostrander EA, Aguirre GD. Linkage analysis and comparative mapping of canine progressive rod-cone degeneration (prcd) establishes potential locus homology with retinitis pigmentosa (RP17) in humans. Proc Natl Acad Sci U S A. 1998 Mar 17;95(6):3048–3053. [PMC free article] [PubMed]
  • Bell GI, Karam JH, Rutter WJ. Polymorphic DNA region adjacent to the 5' end of the human insulin gene. Proc Natl Acad Sci U S A. 1981 Sep;78(9):5759–5763. [PMC free article] [PubMed]
  • Bihoreau MT, Gauguier D, Kato N, Hyne G, Lindpaintner K, Rapp JP, James MR, Lathrop GM. A linkage map of the rat genome derived from three F2 crosses. Genome Res. 1997 May;7(5):434–440. [PubMed]
  • Botstein D, White RL, Skolnick M, Davis RW. Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am J Hum Genet. 1980 May;32(3):314–331. [PMC free article] [PubMed]
  • Mellersh CS, Langston AA, Acland GM, Fleming MA, Ray K, Wiegand NA, Francisco LV, Gibbs M, Aguirre GD, Ostrander EA. A linkage map of the canine genome. Genomics. 1997 Dec 15;46(3):326–336. [PubMed]
  • Broman KW, Murray JC, Sheffield VC, White RL, Weber JL. Comprehensive human genetic maps: individual and sex-specific variation in recombination. Am J Hum Genet. 1998 Sep;63(3):861–869. [PMC free article] [PubMed]
  • Buetow KH. Influence of aberrant observations on high-resolution linkage analysis outcomes. Am J Hum Genet. 1991 Nov;49(5):985–994. [PMC free article] [PubMed]
  • Nadeau JH, Grant PL, Mankala S, Reiner AH, Richardson JE, Eppig JT. A Rosetta stone of mammalian genetics. Nature. 1995 Jan 26;373(6512):363–365. [PubMed]
  • Chakravarti A, Lasher LK, Reefer JE. A maximum likelihood method for estimating genome length using genetic linkage data. Genetics. 1991 May;128(1):175–182. [PMC free article] [PubMed]
  • de Gortari MJ, Freking BA, Cuthbertson RP, Kappes SM, Keele JW, Stone RT, Leymaster KA, Dodds KG, Crawford AM, Beattie CW. A second-generation linkage map of the sheep genome. Mamm Genome. 1998 Mar;9(3):204–209. [PubMed]
  • Deschênes SM, Puck JM, Dutra AS, Somberg RL, Felsburg PJ, Henthorn PS. Comparative mapping of canine and human proximal Xq and genetic analysis of canine X-linked severe combined immunodeficiency. Genomics. 1994 Sep 1;23(1):62–68. [PubMed]
  • Ostrander EA, Sprague GF, Jr, Rine J. Identification and characterization of dinucleotide repeat (CA)n markers for genetic mapping in dog. Genomics. 1993 Apr;16(1):207–213. [PubMed]
  • Dib C, Fauré S, Fizames C, Samson D, Drouot N, Vignal A, Millasseau P, Marc S, Hazan J, Seboun E, et al. A comprehensive genetic map of the human genome based on 5,264 microsatellites. Nature. 1996 Mar 14;380(6570):152–154. [PubMed]
  • Robic A, Milan D, Woloszyn N, Riquet J, Yerle M, Nagel M, Bonnet M, Pinton P, Dalens M, Gellin J. Contribution to the physically anchored linkage map of the pig. Anim Genet. 1997 Apr;28(2):94–102. [PubMed]
  • Dietrich WF, Miller J, Steen R, Merchant MA, Damron-Boles D, Husain Z, Dredge R, Daly MJ, Ingalls KA, O'Connor TJ. A comprehensive genetic map of the mouse genome. Nature. 1996 Mar 14;380(6570):149–152. [PubMed]
  • Selden JR, Moorhead PS, Oehlert ML, Patterson DF. The Giemsa banding pattern of the canine karyotype. Cytogenet Cell Genet. 1975;15(6):380–387. [PubMed]
  • Francisco LV, Langston AA, Mellersh CS, Neal CL, Ostrander EA. A class of highly polymorphic tetranucleotide repeats for canine genetic mapping. Mamm Genome. 1996 May;7(5):359–362. [PubMed]
  • Shibuya H, Collins BK, Huang TH, Johnson GS. A polymorphic (AGGAAT)n tandem repeat in an intron of the canine von Willebrand factor gene. Anim Genet. 1994 Apr;25(2):122–122. [PubMed]
  • Francisco LV, Langston AA, Mellersh CS, Neal CL, Ostrander EA. A class of highly polymorphic tetranucleotide repeats for canine genetic mapping. Mamm Genome. 1996 May;7(5):359–362. [PubMed]
  • Shuber AP, Grondin VJ, Klinger KW. A simplified procedure for developing multiplex PCRs. Genome Res. 1995 Dec;5(5):488–493. [PubMed]
  • Fredholm M, Winterø AK. Variation of short tandem repeats within and between species belonging to the Canidae family. Mamm Genome. 1995 Jan;6(1):11–18. [PubMed]
  • Slonim D, Kruglyak L, Stein L, Lander E. Building human genome maps with radiation hybrids. J Comput Biol. 1997 Winter;4(4):487–504. [PubMed]
  • Gyapay G, Schmitt K, Fizames C, Jones H, Vega-Czarny N, Spillett D, Muselet D, Prud'homme JF, Dib C, Auffray C, et al. A radiation hybrid map of the human genome. Hum Mol Genet. 1996 Mar;5(3):339–346. [PubMed]
  • Stanford JL, Just JJ, Gibbs M, Wicklund KG, Neal CL, Blumenstein BA, Ostrander EA. Polymorphic repeats in the androgen receptor gene: molecular markers of prostate cancer risk. Cancer Res. 1997 Mar 15;57(6):1194–1198. [PubMed]
  • Stewart EA, McKusick KB, Aggarwal A, Bajorek E, Brady S, Chu A, Fang N, Hadley D, Harris M, Hussain S, et al. An STS-based radiation hybrid map of the human genome. Genome Res. 1997 May;7(5):422–433. [PubMed]
  • Holmes NG, Mellersh CS, Humphreys SJ, Binns MM, Holliman A, Curtis R, Sampson J. Isolation and characterization of microsatellites from the canine genome. Anim Genet. 1993 Aug;24(4):289–292. [PubMed]
  • Switoński M, Reimann N, Bosma AA, Long S, Bartnitzke S, Pieńkowska A, Moreno-Milan MM, Fischer P. Report on the progress of standardization of the G-banded canine (Canis familiaris) karyotype. Committee for the Standardized Karyotype of the Dog (Canis familiaris). Chromosome Res. 1996 Jun;4(4):306–309. [PubMed]
  • Holmes NG, Strange NJ, Binns MM, Mellersh CS, Sampson J. Three polymorphic canine microsatellites. Anim Genet. 1994 Jun;25(3):200–200. [PubMed]
  • Thomas R, Holmes NG, Fischer PE, Dickens HF, Breen M, Sampson J, Binns MM. Eight canine microsatellites. Anim Genet. 1997 Apr;28(2):153–154. [PubMed]
  • Holmes NG, Dickens HF, Parker HL, Binns MM, Mellersh CS, Sampson J. Eighteen canine microsatellites. Anim Genet. 1995 Apr;26(2):132–133. [PubMed]
  • Wang DG, Fan JB, Siao CJ, Berno A, Young P, Sapolsky R, Ghandour G, Perkins N, Winchester E, Spencer J, et al. Large-scale identification, mapping, and genotyping of single-nucleotide polymorphisms in the human genome. Science. 1998 May 15;280(5366):1077–1082. [PubMed]
  • Hung T, Mak K, Fong K. A specificity enhancer for polymerase chain reaction. Nucleic Acids Res. 1990 Aug 25;18(16):4953–4953. [PMC free article] [PubMed]
  • Kappes SM, Keele JW, Stone RT, McGraw RA, Sonstegard TS, Smith TP, Lopez-Corrales NL, Beattie CW. A second-generation linkage map of the bovine genome. Genome Res. 1997 Mar;7(3):235–249. [PubMed]
  • Werner P, Raducha MG, Prociuk U, Henthorn PS, Patterson DF. Physical and linkage mapping of human chromosome 17 loci to dog chromosomes 9 and 5. Genomics. 1997 May 15;42(1):74–82. [PubMed]
  • Lincoln SE, Lander ES. Systematic detection of errors in genetic linkage data. Genomics. 1992 Nov;14(3):604–610. [PubMed]
  • Lingaas F, Sorensen A, Juneja RK, Johansson S, Fredholm M, Wintero AK, Sampson J, Mellersh C, Curzon A, Holmes NG, et al. Towards construction of a canine linkage map: establishment of 16 linkage groups. Mamm Genome. 1997 Mar;8(3):218–221. [PubMed]
  • Yuzbasiyan-Gurkan V, Blanton SH, Cao Y, Ferguson P, Li J, Venta PJ, Brewer GJ. Linkage of a microsatellite marker to the canine copper toxicosis locus in Bedlington terriers. Am J Vet Res. 1997 Jan;58(1):23–27. [PubMed]
  • Marklund L, Johansson Moller M, Høyheim B, Davies W, Fredholm M, Juneja RK, Mariani P, Coppieters W, Ellegren H, Andersson L. A comprehensive linkage map of the pig based on a wild pig-Large White intercross. Anim Genet. 1996 Aug;27(4):255–269. [PubMed]

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