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Nucleic Acids Res. Aug 1, 1996; 24(15): 3031–3039.
PMCID: PMC146042

Covalent attachment of synthetic DNA to self-assembled monolayer films.

Abstract

The covalent attachment of thiol-modified DNA oligomers; to self-assembled monolayer silane films on fused silica and oxidized silicon substrates is described. A heterobifunctional crosslinking molecule bearing both thiol- and amino-reactive moieties was used to tether a DNA oligomer (modified at its terminus with a thiol group) to an aminosilane film formed on silica surfaces. A variety of aminosilanes, crosslinkers and treatment conditions have been tested to identify optimal conditions for DNA immobilization using this approach. The DNA films which result have been characterized using UV spectroscopy, water contact angle measurement, radiolabeling and hybridization methods.

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Selected References

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  • Lipshutz RJ, Morris D, Chee M, Hubbell E, Kozal MJ, Shah N, Shen N, Yang R, Fodor SP. Using oligonucleotide probe arrays to access genetic diversity. Biotechniques. 1995 Sep;19(3):442–447. [PubMed]
  • Guo Z, Guilfoyle RA, Thiel AJ, Wang R, Smith LM. Direct fluorescence analysis of genetic polymorphisms by hybridization with oligonucleotide arrays on glass supports. Nucleic Acids Res. 1994 Dec 11;22(24):5456–5465. [PMC free article] [PubMed]
  • Drmanac R, Drmanac S, Strezoska Z, Paunesku T, Labat I, Zeremski M, Snoddy J, Funkhouser WK, Koop B, Hood L, et al. DNA sequence determination by hybridization: a strategy for efficient large-scale sequencing. Science. 1993 Jun 11;260(5114):1649–1652. [PubMed]
  • Southern EM, Case-Green SC, Elder JK, Johnson M, Mir KU, Wang L, Williams JC. Arrays of complementary oligonucleotides for analysing the hybridisation behaviour of nucleic acids. Nucleic Acids Res. 1994 Apr 25;22(8):1368–1373. [PMC free article] [PubMed]
  • Lee GU, Chrisey LA, Colton RJ. Direct measurement of the forces between complementary strands of DNA. Science. 1994 Nov 4;266(5186):771–773. [PubMed]
  • Lamture JB, Beattie KL, Burke BE, Eggers MD, Ehrlich DJ, Fowler R, Hollis MA, Kosicki BB, Reich RK, Smith SR, et al. Direct detection of nucleic acid hybridization on the surface of a charge coupled device. Nucleic Acids Res. 1994 Jun 11;22(11):2121–2125. [PMC free article] [PubMed]
  • Pease AC, Solas D, Sullivan EJ, Cronin MT, Holmes CP, Fodor SP. Light-generated oligonucleotide arrays for rapid DNA sequence analysis. Proc Natl Acad Sci U S A. 1994 May 24;91(11):5022–5026. [PMC free article] [PubMed]
  • Matson RS, Rampal JB, Coassin PJ. Biopolymer synthesis on polypropylene supports. I. Oligonucleotides. Anal Biochem. 1994 Mar;217(2):306–310. [PubMed]
  • Chrisey LA, O'Ferrall CE, Spargo BJ, Dulcey CS, Calvert JM. Fabrication of patterned DNA surfaces. Nucleic Acids Res. 1996 Aug 1;24(15):3040–3047. [PMC free article] [PubMed]
  • Stenger DA, Pike CJ, Hickman JJ, Cotman CW. Surface determinants of neuronal survival and growth on self-assembled monolayers in culture. Brain Res. 1993 Dec 10;630(1-2):136–147. [PubMed]
  • Vargo TG, Gardella JA, Jr, Calvert JM, Chen MS. Adhesive electroless metallization of fluoropolymeric substrates. Science. 1993 Dec 10;262(5140):1711–1712. [PubMed]
  • Maskos U, Southern EM. Oligonucleotide hybridizations on glass supports: a novel linker for oligonucleotide synthesis and hybridization properties of oligonucleotides synthesised in situ. Nucleic Acids Res. 1992 Apr 11;20(7):1679–1684. [PMC free article] [PubMed]
  • Bhatia SK, Teixeira JL, Anderson M, Shriver-Lake LC, Calvert JM, Georger JH, Hickman JJ, Dulcey CS, Schoen PE, Ligler FS. Fabrication of surfaces resistant to protein adsorption and application to two-dimensional protein patterning. Anal Biochem. 1993 Jan;208(1):197–205. [PubMed]
  • Ghosh SS, Musso GF. Covalent attachment of oligonucleotides to solid supports. Nucleic Acids Res. 1987 Jul 10;15(13):5353–5372. [PMC free article] [PubMed]

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