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Genetics. Nov 1998; 150(3): 1217–1228.
PMCID: PMC1460378

Comparative mapping between Arabidopsis thaliana and Brassica nigra indicates that Brassica genomes have evolved through extensive genome replication accompanied by chromosome fusions and frequent rearrangements.

Abstract

Chromosome organization and evolution in the Brassicaceae family was studied using comparative linkage mapping. A total of 160 mapped Arabidopsis thaliana DNA fragments identified 284 homologous loci covering 751 cM in Brassica nigra. The data support that modern diploid Brassica species are descended from a hexaploid ancestor, and that the A. thaliana genome is similar in structure and complexity to those of each of the hypothetical diploid progenitors of the proposed hexaploid. Thus, the Brassica lineage probably went through a triplication after the divergence of the lineages leading to A. thaliana and B. nigra. These duplications were also accompanied by an exceptionally high rate of chromosomal rearrangements. The average length of conserved segments between A. thaliana and B. nigra was estimated at 8 cM. This estimate corresponds to approximately 90 rearrangements since the divergence of the two species. The estimated rate of chromosomal rearrangements is higher than any previously reported data based on comparative mapping. Despite the large number of rearrangements, fine-scale comparative mapping between model plant A. thaliana and Brassica crops is likely to result in the identification of a large number of genes that affect important traits in Brassica crops.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Ahn S, Tanksley SD. Comparative linkage maps of the rice and maize genomes. Proc Natl Acad Sci U S A. 1993 Sep 1;90(17):7980–7984. [PMC free article] [PubMed]
  • Ahn S, Anderson JA, Sorrells ME, Tanksley SD. Homoeologous relationships of rice, wheat and maize chromosomes. Mol Gen Genet. 1993 Dec;241(5-6):483–490. [PubMed]
  • Barendse W, Armitage SM, Kossarek LM, Shalom A, Kirkpatrick BW, Ryan AM, Clayton D, Li L, Neibergs HL, Zhang N, et al. A genetic linkage map of the bovine genome. Nat Genet. 1994 Mar;6(3):227–235. [PubMed]
  • Bariola PA, Howard CJ, Taylor CB, Verburg MT, Jaglan VD, Green PJ. The Arabidopsis ribonuclease gene RNS1 is tightly controlled in response to phosphate limitation. Plant J. 1994 Nov;6(5):673–685. [PubMed]
  • Lagercrantz U, Lydiate DJ. RFLP mapping in Brassica nigra indicates differing recombination rates in male and female meioses. Genome. 1995 Apr;38(2):255–264. [PubMed]
  • Barnett MA, Buckle VJ, Evans EP, Porter AC, Rout D, Smith AG, Brown WR. Telomere directed fragmentation of mammalian chromosomes. Nucleic Acids Res. 1993 Jan 11;21(1):27–36. [PMC free article] [PubMed]
  • Lagercrantz U, Lydiate DJ. Comparative genome mapping in Brassica. Genetics. 1996 Dec;144(4):1903–1910. [PMC free article] [PubMed]
  • Bertoni L, Attolini C, Tessera L, Mucciolo E, Giulotto E. Telomeric and nontelomeric (TTAGGG)n sequences in gene amplification and chromosome stability. Genomics. 1994 Nov 1;24(1):53–62. [PubMed]
  • Bevan M, Bancroft I, Bent E, Love K, Goodman H, Dean C, Bergkamp R, Dirkse W, Van Staveren M, Stiekema W, et al. Analysis of 1.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis thaliana. Nature. 1998 Jan 29;391(6666):485–488. [PubMed]
  • Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newberg LA, Newburg L. MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics. 1987 Oct;1(2):174–181. [PubMed]
  • Cavell AC, Lydiate DJ, Parkin IA, Dean C, Trick M. Collinearity between a 30-centimorgan segment of Arabidopsis thaliana chromosome 4 and duplicated regions within the Brassica napus genome. Genome. 1998 Feb;41(1):62–69. [PubMed]
  • Chang C, Bowman JL, DeJohn AW, Lander ES, Meyerowitz EM. Restriction fragment length polymorphism linkage map for Arabidopsis thaliana. Proc Natl Acad Sci U S A. 1988 Sep;85(18):6856–6860. [PMC free article] [PubMed]
  • Chen Q, Armstrong K. Genomic in situ hybridization in Avena sativa. Genome. 1994 Aug;37(4):607–612. [PubMed]
  • Liu YG, Mitsukawa N, Lister C, Dean C, Whittier RF. Isolation and mapping of a new set of 129 RFLP markers in Arabidopsis thaliana using recombinant inbred lines. Plant J. 1996 Oct;10(4):733–736. [PubMed]
  • Copeland NG, Jenkins NA, Gilbert DJ, Eppig JT, Maltais LJ, Miller JC, Dietrich WF, Weaver A, Lincoln SE, Steen RG, et al. A genetic linkage map of the mouse: current applications and future prospects. Science. 1993 Oct 1;262(5130):57–66. [PubMed]
  • Ecker JR. Genome sequencing. Genes blossom from a weed. Nature. 1998 Jan 29;391(6666):438–439. [PubMed]
  • Ehrlich J, Sankoff D, Nadeau JH. Synteny conservation and chromosome rearrangements during mammalian evolution. Genetics. 1997 Sep;147(1):289–296. [PMC free article] [PubMed]
  • Ma H, Yanofsky MF, Meyerowitz EM. Molecular cloning and characterization of GPA1, a G protein alpha subunit gene from Arabidopsis thaliana. Proc Natl Acad Sci U S A. 1990 May;87(10):3821–3825. [PMC free article] [PubMed]
  • Eldridge MD, Close RL. Radiation of chromosome shuffles. Curr Opin Genet Dev. 1993 Dec;3(6):915–922. [PubMed]
  • Masterson J. Stomatal size in fossil plants: evidence for polyploidy in majority of angiosperms. Science. 1994 Apr 15;264(5157):421–424. [PubMed]
  • Matsui M, Sasamoto S, Kunieda T, Nomura N, Ishizaki R. Cloning of ara, a putative Arabidopsis thaliana gene homologous to the ras-related gene family. Gene. 1989;76(2):313–319. [PubMed]
  • Feinbaum RL, Ausubel FM. Transcriptional regulation of the Arabidopsis thaliana chalcone synthase gene. Mol Cell Biol. 1988 May;8(5):1985–1992. [PMC free article] [PubMed]
  • Gaut BS, Doebley JF. DNA sequence evidence for the segmental allotetraploid origin of maize. Proc Natl Acad Sci U S A. 1997 Jun 24;94(13):6809–6814. [PMC free article] [PubMed]
  • Graves JA. Mammals that break the rules: genetics of marsupials and monotremes. Annu Rev Genet. 1996;30:233–260. [PubMed]
  • Meyne J, Baker RJ, Hobart HH, Hsu TC, Ryder OA, Ward OG, Wiley JE, Wurster-Hill DH, Yates TL, Moyzis RK. Distribution of non-telomeric sites of the (TTAGGG)n telomeric sequence in vertebrate chromosomes. Chromosoma. 1990 Apr;99(1):3–10. [PubMed]
  • Hastie ND, Allshire RC. Human telomeres: fusion and interstitial sites. Trends Genet. 1989 Oct;5(10):326–331. [PubMed]
  • Mindrinos M, Katagiri F, Yu GL, Ausubel FM. The A. thaliana disease resistance gene RPS2 encodes a protein containing a nucleotide-binding site and leucine-rich repeats. Cell. 1994 Sep 23;78(6):1089–1099. [PubMed]
  • Helentjaris T, Weber D, Wright S. Identification of the genomic locations of duplicate nucleotide sequences in maize by analysis of restriction fragment length polymorphisms. Genetics. 1988 Feb;118(2):353–363. [PMC free article] [PubMed]
  • Janke A, Feldmaier-Fuchs G, Thomas WK, von Haeseler A, Päbo S. The marsupial mitochondrial genome and the evolution of placental mammals. Genetics. 1994 May;137(1):243–256. [PMC free article] [PubMed]
  • Moore G, Devos KM, Wang Z, Gale MD. Cereal genome evolution. Grasses, line up and form a circle. Curr Biol. 1995 Jul 1;5(7):737–739. [PubMed]
  • Jellen EN, Gill BS, Cox TS. Genomic in situ hybridization differentiates between A/D- and C-genome chromatin and detects intergenomic translocations in polyploid oat species (genus Avena). Genome. 1994 Aug;37(4):613–618. [PubMed]
  • Johansson M, Ellegren H, Andersson L. Comparative mapping reveals extensive linkage conservation--but with gene order rearrangements--between the pig and the human genomes. Genomics. 1995 Feb 10;25(3):682–690. [PubMed]
  • O'Brien SJ, Seuánez HN, Womack JE. Mammalian genome organization: an evolutionary view. Annu Rev Genet. 1988;22:323–351. [PubMed]
  • Kenton A, Parokonny AS, Gleba YY, Bennett MD. Characterization of the Nicotiana tabacum L. genome by molecular cytogenetics. Mol Gen Genet. 1993 Aug;240(2):159–169. [PubMed]
  • Osborn TC, Kole C, Parkin IA, Sharpe AG, Kuiper M, Lydiate DJ, Trick M. Comparison of flowering time genes in Brassica rapa, B. napus and Arabidopsis thaliana. Genetics. 1997 Jul;146(3):1123–1129. [PMC free article] [PubMed]
  • Konieczny A, Ausubel FM. A procedure for mapping Arabidopsis mutations using co-dominant ecotype-specific PCR-based markers. Plant J. 1993 Aug;4(2):403–410. [PubMed]
  • Parkin IA, Sharpe AG, Keith DJ, Lydiate DJ. Identification of the A and C genomes of amphidiploid Brassica napus (oilseed rape). Genome. 1995 Dec;38(6):1122–1131. [PubMed]
  • Paterson AH, Lan TH, Reischmann KP, Chang C, Lin YR, Liu SC, Burow MD, Kowalski SP, Katsar CS, DelMonte TA, et al. Toward a unified genetic map of higher plants, transcending the monocot-dicot divergence. Nat Genet. 1996 Dec;14(4):380–382. [PubMed]
  • Song K, Lu P, Tang K, Osborn TC. Rapid genome change in synthetic polyploids of Brassica and its implications for polyploid evolution. Proc Natl Acad Sci U S A. 1995 Aug 15;92(17):7719–7723. [PMC free article] [PubMed]
  • Prince JP, Pochard E, Tanksley SD. Construction of a molecular linkage map of pepper and a comparison of synteny with tomato. Genome. 1993 Jun;36(3):404–417. [PubMed]
  • Reinisch AJ, Dong JM, Brubaker CL, Stelly DM, Wendel JF, Paterson AH. A detailed RFLP map of cotton, Gossypium hirsutum x Gossypium barbadense: chromosome organization and evolution in a disomic polyploid genome. Genetics. 1994 Nov;138(3):829–847. [PMC free article] [PubMed]
  • Tanksley SD, Ganal MW, Prince JP, de Vicente MC, Bonierbale MW, Broun P, Fulton TM, Giovannoni JJ, Grandillo S, Martin GB, et al. High density molecular linkage maps of the tomato and potato genomes. Genetics. 1992 Dec;132(4):1141–1160. [PMC free article] [PubMed]
  • Richards EJ, Ausubel FM. Isolation of a higher eukaryotic telomere from Arabidopsis thaliana. Cell. 1988 Apr 8;53(1):127–136. [PubMed]
  • Tanksley SD, Bernatzky R, Lapitan NL, Prince JP. Conservation of gene repertoire but not gene order in pepper and tomato. Proc Natl Acad Sci U S A. 1988 Sep;85(17):6419–6423. [PMC free article] [PubMed]
  • Wendel JF. New World tetraploid cottons contain Old World cytoplasm. Proc Natl Acad Sci U S A. 1989 Jun;86(11):4132–4136. [PMC free article] [PubMed]
  • Whitkus R, Doebley J, Lee M. Comparative genome mapping of Sorghum and maize. Genetics. 1992 Dec;132(4):1119–1130. [PMC free article] [PubMed]
  • Shih MC, Heinrich P, Goodman HM. Cloning and chromosomal mapping of nuclear genes encoding chloroplast and cytosolic glyceraldehyde-3-phosphate-dehydrogenase from Arabidopsis thaliana. Gene. 1991 Aug 15;104(2):133–138. [PubMed]
  • Wilson AC, Sarich VM, Maxson LR. The importance of gene rearrangement in evolution: evidence from studies on rates of chromosomal, protein, and anatomical evolution. Proc Natl Acad Sci U S A. 1974 Aug;71(8):3028–3030. [PMC free article] [PubMed]
  • Slijepcevic P, Xiao Y, Dominguez I, Natarajan AT. Spontaneous and radiation-induced chromosomal breakage at interstitial telomeric sites. Chromosoma. 1996 Jun;104(8):596–604. [PubMed]
  • Yanofsky MF, Ma H, Bowman JL, Drews GN, Feldmann KA, Meyerowitz EM. The protein encoded by the Arabidopsis homeotic gene agamous resembles transcription factors. Nature. 1990 Jul 5;346(6279):35–39. [PubMed]

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