• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of geneticsGeneticsCurrent IssueInformation for AuthorsEditorial BoardSubscribeSubmit a Manuscript
Genetics. Jul 1998; 149(3): 1363–1376.
PMCID: PMC1460245

The Y chromosomal fertility factor Threads in Drosophila hydei harbors a functional gene encoding an axonemal dynein beta heavy chain protein.

Abstract

To understand the contradiction between megabase-sized lampbrush loops and putative protein encoding genes both associated with the loci of Y chromosomal fertility genes of Drosophila on the molecular level, we used PCR-mediated cloning to identify and isolate the cDNA sequence of the Y chromosomal Drosophila hydei gene DhDhc7(Y). Alignment of the sequences of the putative protein DhDhc7(Y) and the outer arm dynein beta heavy chain protein DYH2 of Tripneustes gratilla shows homology over the entire length of the protein chains. Therefore the proteins can be assumed to fulfill orthologous functions within the sperm tail axonemes of both species. Functional dynein beta heavy chain molecules, however, are necessary for the assembly and attachment of outer dynein arms within the sperm tail axoneme. Localization of DhDhc7(Y) to the fertility factor Threads, comprising at least 5.1 Mb of transcriptionally active repetitive DNA, results from an infertile Threads- mutant where large clusters of Threads specifically transcribed satellites and parts of DhDhc7(Y) encoding sequences are missing simultaneously. Consequently, the complete lack of the outer dynein arms in Threads- males most probably causes sperm immotility and hence infertility of the fly. Moreover, preliminary sequence analysis and several other features support the hypothesis that DhDhc7(Y) on the lampbrush loops Threads in D. hydei and Dhc-Yh3 on the lampbrush loops kl-5 in Drosophila melanogaster on the heterochromatic Y chromosome of both species might indeed code for orthologous dynein beta heavy chain proteins.

Full Text

The Full Text of this article is available as a PDF (357K).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Beck H. New compound (1) chromosomes and the production of large quantities of X/O males in Drosophila hydei. Genet Res. 1975 Dec;26(3):313–317. [PubMed]
  • Bonaccorsi S, Lohe A. Fine mapping of satellite DNA sequences along the Y chromosome of Drosophila melanogaster: relationships between satellite sequences and fertility factors. Genetics. 1991 Sep;129(1):177–189. [PMC free article] [PubMed]
  • Bonaccorsi S, Pimpinelli S, Gatti M. Cytological dissection of sex chromosome heterochromatin of Drosophila hydei. Chromosoma. 1981;84(3):391–403. [PubMed]
  • Bonaccorsi S, Pisano C, Puoti F, Gatti M. Y chromosome loops in Drosophila melanogaster. Genetics. 1988 Dec;120(4):1015–1034. [PMC free article] [PubMed]
  • Bonaccorsi S, Gatti M, Pisano C, Lohe A. Transcription of a satellite DNA on two Y chromosome loops of Drosophila melanogaster. Chromosoma. 1990 Aug;99(4):260–266. [PubMed]
  • Hess O. Complementation of genetic activity in translocated fragmentsof the Y chromosome in Drosophila hydei. Genetics. 1967 Jun;56(2):283–295. [PMC free article] [PubMed]
  • Bridges CB. Non-Disjunction as Proof of the Chromosome Theory of Heredity (Concluded). Genetics. 1916 Mar;1(2):107–163. [PMC free article] [PubMed]
  • Brosseau GE. Genetic Analysis of the Male Fertility Factors on the Y Chromosome of Drosophila Melanogaster. Genetics. 1960 Mar;45(3):257–274. [PMC free article] [PubMed]
  • Huijser P, Kirchhoff C, Lankenau DH, Hennig W. Retrotransposon-like sequences are expressed in Y chromosomal lampbrush loops of Drosophila hydei. J Mol Biol. 1988 Oct 5;203(3):689–697. [PubMed]
  • Devlin RH, Bingham B, Wakimoto BT. The organization and expression of the light gene, a heterochromatic gene of Drosophila melanogaster. Genetics. 1990 May;125(1):129–140. [PMC free article] [PubMed]
  • Judd BH, Shen MW, Kaufman TC. The anatomy and function of a segment of the X chromosome of Drosophila melanogaster. Genetics. 1972 May;71(1):139–156. [PMC free article] [PubMed]
  • Devlin RH, Holm DG, Morin KR, Honda BM. Identifying a single-copy DNA sequence associated with the expression of a heterochromatic gene, the light locus of Drosophila melanogaster. Genome. 1990 Jun;33(3):405–415. [PubMed]
  • Kawasaki ES, Clark SS, Coyne MY, Smith SD, Champlin R, Witte ON, McCormick FP. Diagnosis of chronic myeloid and acute lymphocytic leukemias by detection of leukemia-specific mRNA sequences amplified in vitro. Proc Natl Acad Sci U S A. 1988 Aug;85(15):5698–5702. [PMC free article] [PubMed]
  • Diaz MO, Barsacchi-Pilone G, Mahon KA, Gall JG. Transcripts from both strands of a satellite DNA occur on lampbrush chromosome loops of the newt Notophthalmus. Cell. 1981 Jun;24(3):649–659. [PubMed]
  • Kennison JA. The Genetic and Cytological Organization of the Y Chromosome of DROSOPHILA MELANOGASTER. Genetics. 1981 Jul;98(3):529–548. [PMC free article] [PubMed]
  • Dutcher SK. Flagellar assembly in two hundred and fifty easy-to-follow steps. Trends Genet. 1995 Oct;11(10):398–404. [PubMed]
  • Kennison JA. Analysis of Y-Linked Mutations to Male Sterility in DROSOPHILA MELANOGASTER. Genetics. 1983 Feb;103(2):219–234. [PMC free article] [PubMed]
  • Koonce MP, Grissom PM, McIntosh JR. Dynein from Dictyostelium: primary structure comparisons between a cytoplasmic motor enzyme and flagellar dynein. J Cell Biol. 1992 Dec;119(6):1597–1604. [PMC free article] [PubMed]
  • Gatti M, Pimpinelli S. Functional elements in Drosophila melanogaster heterochromatin. Annu Rev Genet. 1992;26:239–275. [PubMed]
  • Kurek R, Trapitz P, Bünemann H. Strukturdifferenzierungen in Y-chromosom von Drosophila hydei: the unique morphology of the Y chromosomal lampbrush loops Threads results from 'coaxial shells' formed by different satellite-specific subregions within megabase-sized transcripts. Chromosome Res. 1996 Feb;4(2):87–102. [PubMed]
  • Gepner J, Hays TS. A fertility region on the Y chromosome of Drosophila melanogaster encodes a dynein microtubule motor. Proc Natl Acad Sci U S A. 1993 Dec 1;90(23):11132–11136. [PMC free article] [PubMed]
  • Lankenau DH, Huijser P, Hennig W. Characterization of the long terminal repeats of micropia elements microdissected from the Y-chromosomal lampbrush loops "threads" of Drosophila hydei. J Mol Biol. 1989 Oct 5;209(3):493–497. [PubMed]
  • Gibbons IR, Gibbons BH, Mocz G, Asai DJ. Multiple nucleotide-binding sites in the sequence of dynein beta heavy chain. Nature. 1991 Aug 15;352(6336):640–643. [PubMed]
  • Lankenau S, Corces VG, Lankenau DH. The Drosophila micropia retrotransposon encodes a testis-specific antisense RNA complementary to reverse transcriptase. Mol Cell Biol. 1994 Mar;14(3):1764–1775. [PMC free article] [PubMed]
  • Le MH, Duricka D, Karpen GH. Islands of complex DNA are widespread in Drosophila centric heterochromatin. Genetics. 1995 Sep;141(1):283–303. [PMC free article] [PubMed]
  • Goldstein LS, Hardy RW, Lindsley DL. Structural genes on the Y chromosome of Drosophila melanogaster. Proc Natl Acad Sci U S A. 1982 Dec;79(23):7405–7409. [PMC free article] [PubMed]
  • Mitchell DR, Brown KS. Sequence analysis of the Chlamydomonas alpha and beta dynein heavy chain genes. J Cell Sci. 1994 Mar;107(Pt 3):635–644. [PubMed]
  • Hackstein JH. Spermatogenesis in Drosophila. Results Probl Cell Differ. 1987;15:63–116. [PubMed]
  • Rasmusson K, Serr M, Gepner J, Gibbons I, Hays TS. A family of dynein genes in Drosophila melanogaster. Mol Biol Cell. 1994 Jan;5(1):45–55. [PMC free article] [PubMed]
  • Hackstein JH, Leoncini O, Beck H, Peelen G, Hennig W. Genetic fine structure of the Y chromosome of Drosophila hydei. Genetics. 1982 Jun;101(2):257–277. [PMC free article] [PubMed]
  • Rudenko GN, Rommens CM, Nijkamp HJ, Hille J. Supported PCR: an efficient procedure to amplify sequences flanking a known DNA segment. Plant Mol Biol. 1993 Feb;21(4):723–728. [PubMed]
  • Hackstein JH, Hochstenbach R. The elusive fertility genes of Drosophila: the ultimate haven for selfish genetic elements. Trends Genet. 1995 May;11(5):195–200. [PubMed]
  • Scott MP, Weiner AJ, Hazelrigg TI, Polisky BA, Pirrotta V, Scalenghe F, Kaufman TC. The molecular organization of the Antennapedia locus of Drosophila. Cell. 1983 Dec;35(3 Pt 2):763–776. [PubMed]
  • Trapitz P, Wlaschek M, Bünemann H. Structure and function of Y chromosomal DNA. II. Analysis of lampbrush loop associated transcripts in nuclei of primary spermatocytes of Drosophila hydei by in situ hybridization using asymmetric RNA probes of four different families of repetitive DNA. Chromosoma. 1988;96(2):159–170. [PubMed]
  • Hennig W, Brand RC, Hackstein J, Hochstenbach R, Kremer H, Lankenau DH, Lankenau S, Miedema K, Pötgens A. Y chromosomal fertility genes of Drosophila: a new type of eukaryotic genes. Genome. 1989;31(2):561–571. [PubMed]
  • Trapitz P, Glätzer KH, Bünemann H. Towards a physical map of the fertility genes on the heterochromatic Y chromosome of Drosophila hydei: families of repetitive sequences transcribed on the lampbrush loops Nooses and Threads are organized in extended clusters of several hundred kilobases. Mol Gen Genet. 1992 Nov;235(2-3):221–234. [PubMed]
  • Williamson JH. Allelic complementation between mutants in the fertility factyors of the Y chromosome in Drosophila melanogaster. Mol Gen Genet. 1972;119(1):43–47. [PubMed]

Articles from Genetics are provided here courtesy of Genetics Society of America

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

  • Gene
    Gene
    Gene links
  • GEO Profiles
    GEO Profiles
    Related GEO records
  • HomoloGene
    HomoloGene
    HomoloGene links
  • MedGen
    MedGen
    Related information in MedGen
  • Nucleotide
    Nucleotide
    Published Nucleotide sequences
  • Pathways + GO
    Pathways + GO
    Pathways, annotations and biological systems (BioSystems) that cite the current article.
  • Protein
    Protein
    Published protein sequences
  • PubMed
    PubMed
    PubMed citations for these articles
  • Taxonomy
    Taxonomy
    Related taxonomy entry
  • Taxonomy Tree
    Taxonomy Tree

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...